Effects of Magnetic Infrared Laser on Dinitrofluorobenzene-induced Contact Dermatitis in Mice

[Purpose] The purpose of the present study was to investigate the effect of a magnetic infrared laser (MIL) on the 2, 4-dinitrofluorobenzene (DNFB)-induced contact dermatitis, type I allergic mouse model. [Subjects] Male ICR mice. [Methods] Contact dermatitis was induced by sensitization with dinitr...

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Published inJournal of Physical Therapy Science Vol. 23; no. 3; pp. 413 - 417
Main Authors Cheon, Woo-Hyun, Park, Kyung-Won, Back, Young-Doo, Lee, Hyeung-Sik, Ku, Sae-Kwang, Chung, In-Kwon, Chang, Jeong Hyun, Kim, Cheol-Young, Choi, Seong-Hun
Format Journal Article
LanguageEnglish
Published The Society of Physical Therapy Science 2011
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Online AccessGet full text
ISSN0915-5287
2187-5626
2187-5626
DOI10.1589/jpts.23.413

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Abstract [Purpose] The purpose of the present study was to investigate the effect of a magnetic infrared laser (MIL) on the 2, 4-dinitrofluorobenzene (DNFB)-induced contact dermatitis, type I allergic mouse model. [Subjects] Male ICR mice. [Methods] Contact dermatitis was induced by sensitization with dinitrophenyl-derivatized ovalbumin (DNP-OVA) and DNFB challenge as antigen. MIL radiation doses of 1.33, 2.66 and 6.65 J/cm2 were given, once a day for 7 continuous days, to DNP-OVA sensitized mice and compared with 15 mg/kg dexamethasone (intraperitoneal treatment). The edematous changes and scratching behavior were measured together with ear histopathological observations. [Results] Immediately after DNFB challenge to the ear or paw of DNP-OVA sensitized mice, increases of ear and paw thicknesses and weights were detected as well as anterior ear skin (dermis to epidermis) thickness and paw scratching behavior increases. However, these DNFB-induced increases of edematous changes and scratching behavior were significantly and dose-dependently decreased by MIL irradiation. [Conclusion] The results obtained in this study suggest that MIL radiation has relatively favorable effects on allergic dermatitis, and MIL therapy may represent an alternative approach for the treatment of allergic cutaneous diseases.
AbstractList [Purpose] The purpose of the present study was to investigate the effect of a magnetic infrared laser (MIL) on the 2, 4-dinitrofluorobenzene (DNFB)-induced contact dermatitis, type I allergic mouse model. [Subjects] Male ICR mice. [Methods] Contact dermatitis was induced by sensitization with dinitrophenyl-derivatized ovalbumin (DNP-OVA) and DNFB challenge as antigen. MIL radiation doses of 1.33, 2.66 and 6.65 J/cm2 were given, once a day for 7 continuous days, to DNP-OVA sensitized mice and compared with 15 mg/kg dexamethasone (intraperitoneal treatment). The edematous changes and scratching behavior were measured together with ear histopathological observations. [Results] Immediately after DNFB challenge to the ear or paw of DNP-OVA sensitized mice, increases of ear and paw thicknesses and weights were detected as well as anterior ear skin (dermis to epidermis) thickness and paw scratching behavior increases. However, these DNFB-induced increases of edematous changes and scratching behavior were significantly and dose-dependently decreased by MIL irradiation. [Conclusion] The results obtained in this study suggest that MIL radiation has relatively favorable effects on allergic dermatitis, and MIL therapy may represent an alternative approach for the treatment of allergic cutaneous diseases.
「Abstract」. [Purpose] The purpose of the present study was to investigate the effect of a magnetic infrared laser (MIL) on the 2, 4-dinitrofluorobenzene (DNFB)-induced contact dermatitis, type I allergic mouse model. [Subjects] Male ICR mice. [Methods] Contact dermatitis was induced by sensitization with dinitrophenyl-derivatized ovalbumin (DNP-OVA) and DNFB challenge as antigen. MIL radiation doses of 1.33, 2.66 and 6.65 J/cm2 were given, once a day for 7 continuous days, to DNP-OVA sensitized mice and compared with 15 mg/kg dexamethasone (intraperitoneal treatment). The edematous changes and scratching behavior were measured together with ear histopathological observations. [Results] Immediately after DNFB challenge to the ear or paw of DNP-OVA sensitized mice, increases of ear and paw thicknesses and weights were detected as well as anterior ear skin (dermis to epidermis) thickness and paw scratching behavior increases. However, these DNFB-induced increases of edematous changes and scratching behavior were significantly and dose-dependently decreased by MIL irradiation. [Conclusion] The results obtained in this study suggest that MIL radiation has relatively favorable effects on allergic dermatitis, and MIL therapy may represent an alternative approach for the treatment of allergic cutaneous diseases.
Purpose: The purpose of the present study was to investigate the effect of a magnetic infrared laser (MIL) on the 2, 4-dinitrofluorobenzene (DNFB)-induced contact dermatitis, type I allergic mouse model. Subjects: Male ICR mice. Methods: Contact dermatitis was induced by sensitization with dinitrophenyl-derivatized ovalbumin (DNP-OVA) and DNFB challenge as antigen. MIL radiation doses of 1.33, 2.66 and 6.65 J/cm2 were given, once a day for 7 continuous days, to DNP-OVA sensitized mice and compared with 15 mg/kg dexamethasone (intraperitoneal treatment). The edematous changes and scratching behavior were measured together with ear histopathological observations. Results: Immediately after DNFB challenge to the ear or paw of DNP-OVA sensitized mice, increases of ear and paw thicknesses and weights were detected as well as anterior ear skin (dermis to epidermis) thickness and paw scratching behavior increases. However , these DNFB- induced increases of edematous changes and scratching behavior were significantly and dose-dependently decreased by MIL irradiation. Conclusion: The results obtained in this study suggest that MIL radiation has relatively favorable effects on allergic dermatitis, and MIL therapy may represent an alternative approach for the treatment of allergic cutaneous diseases.
Author Ku, Sae-Kwang
Park, Kyung-Won
Cheon, Woo-Hyun
Chang, Jeong Hyun
Choi, Seong-Hun
Lee, Hyeung-Sik
Back, Young-Doo
Chung, In-Kwon
Kim, Cheol-Young
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  fullname: Back, Young-Doo
  organization: Laboratory of Clinical Laboratory Science, Oriental Hospital of Daegu Haany University
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  fullname: Lee, Hyeung-Sik
  organization: Department of Clinical Laboratory Science, College of Health and Therapy, Daegu Haany University
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  fullname: Ku, Sae-Kwang
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  fullname: Chung, In-Kwon
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  fullname: Choi, Seong-Hun
  organization: Department of Anatomy and Histology, College of Oriental Medicine, Daegu Haany University
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Cites_doi 10.1016/S0091-6749(97)70003-4
10.1002/ijc.11002
10.1254/jphs.91.263
10.1589/jpts.20.255
10.1084/jem.98.6.533
10.1248/bpb.22.26
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10.1134/S0006350907050090
10.1016/S0031-9406(10)61694-X
10.4062/biomolther.2008.16.2.137
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References_xml – reference: 21) Rossetti G, Laffitte E, Eigenmann PA, et al.: Treatment of atopic dermatitis: practical approach. Rev Med Suisse, 2005, 1: 501-504.
– reference: 10) Shichinohe K, Shimizu M, Kurokawa K: Effect of M-711 on experimental skin reactions induced by chemical mediators in rats. J Vet Med Sci, 1996, 58: 419-423.
– reference: 4) Nagai H, Matsuo A, Hiyama H, et al.: Immunoglobulin E production in mice by means of contact sensitization with a simple chemical, hapten. J Allergy Clin Immunol, 1997, 100: S39-S44.
– reference: 8) Ganir EM, Capulong MC, Tahara K, et al.: Treatment of atopic dermatitis in children: the importance of skin care and environmental control. Acta Paediatr. Jpn, 1996, 38: 702-704.
– reference: 14) Ziskin MC, Mcdiamid T, Michlovitz SI: Therapeutic ultrasound, In: Thermal Agents in Rehabilitation, Philadelphia: F.A. Davis Co, 1990, pp 134-169.
– reference: 17) Oasevich IA, Shargorodski AG: Low-intensity infrared laser radiation in the diagnosis and combined treatment of acute nonspecific lymphadenitis of the face and neck in children. Stomatologiia. Mosk, 1999, 78: 28-30.
– reference: 1) Wang HW, Tedla N, Lloyd AR, et al.: Mast cell activation and migration to lymph nodes during induction of an immune response in mice. J Clin Invest, 1998, 102: 1617-1626.
– reference: 28) Rudik DV, Tikhomirova EI: A study of the functional activity of macrophages of peritoneal exudate of mice exposed to low-intensity laser radiation in vitro and in vivo. Biofizika, 2007, 52: 931-937.
– reference: 29) Ramprasath VR, Shanthi P, Sachdanandam P: Immunomodulatory and anti-inflammatory effects of Semecarpus anacardium LINN. Nut milk extract in experimental inflammatory conditions. Biol Pharm Bull, 2006, 29: 693-700.
– reference: 24) Watanabe C, Satoh T, Tahara E, et al.: Inhibitory mechanisms of glycoprotein fraction derived from Miscanthus sinensis for the immediate phase response of an IgE-mediated cutaneous reaction. Biol Pharm Bull, 1999, 22: 26-30.
– reference: 13) Bouwhuijsen F: Terapia de onda corta pulsátil y continua, Delft: Enraf-Nonius B.V., 1986, p 35.
– reference: 12) Gersh MR: Transcutaneous electrical nerve stimulation for management of pain, In: Electrotherapy in Rehabilitation, Philadelphia: F.A. Davis Co, 1990, pp 149-196.
– reference: 6) Matsuda H, Tomohiro N, Ido Y, et al.: Anti-allergic effects of cnidii monnieri fructus (dried fruits of Cnidium monnieri) and its major component, osthol. Biol Pharm Bull, 2002, 25: 809-812.
– reference: 26) Mikaelian NP, Stupin IV, Il'ina EI: Correction of metabolic disorders by magnetic-laser irradiation (experimental study). Khirurgiia. Mosk, 1990, 10: 86-89.
– reference: 3) Nagai H, Hiyama H, Matsuo A, et al.: FK-506 and cyclosporin A potentiate the IgE antibody production by contact sensitization with hapten in mice. J Pharmacol Exp Ther, 1997, 283: 321-327.
– reference: 11) Ohtsuka E, Kawai S, Nojima H, et al.: Inhibitory effect of azelastine on allergic itch-associated response in mice sensitized with mosquito salivary glands extract. J Pharmacol Sci, 2003, 91: 263-266.
– reference: 19) Cho KH, Kim HD, Lee BW, et al.: Effects of magnetic infrared laser on xylene-induced acute inflammation in mice. J Phys Ther Sci, 2008, 20: 255-259.
– reference: 22) Park JH, Lee SN, Ku SK: Effect of Picrorrhiza Rhizoma on dinitrofluorobenzene-induced contact dermatitis (Type I allergy). Biomol & Therap, 2008, 16: 237-242.
– reference: 2) Perez A, Narayan S, Sansom J: Occupational contact dermatitis from 2,4-dinitrofluorobenzene. Contact Dermatitis, 2004, 51: 314.
– reference: 7) Gupta AK, Chow M: Prednicarbate (Dermatop): profile of a corticosteroid. J Cutan Med Surg, 2004, 8: 244-247.
– reference: 18) Koudinova NV, Pinthus JH, Brandis A, et al.: Photodynamic therapy with Pd-Bacteriopheophorbide (TOOKAD): successful in vivo treatment of human prostatic small cell carcinoma xenografts. Int J Cancer, 2003, 104: 782-789.
– reference: 20) Skinner R: Role of topical therapies in the management of cutaneous disease. J Cutan Med Surg, 2004, 8: 22-31.
– reference: 16) Makarenkov VV, Shargorodski AG: The prevention of inflammatory complications in mandibular fractures by using infrared laser and magnetic-laser radiation Stomatologiia (Mosk), 1998, 77: 20-22.
– reference: 5) Ueda Y, Sone T, Inagaki N, et al.: Effects of prednisolone on the cutaneous reaction and skin barrier function in mice treated with a hapten. Biol Pharm Bull, 2003, 26: 618-621.
– reference: 25) Lee HS, Ku SK: Effects of Picrorrhiza Rhizoma on acute inflammation in mice. Biomol & Therap, 2008, 16: 137-140.
– reference: 23) Eisen HN, Belmam S: Studies of hypersensitivity to low molecular weight substances II. Reactions of some allergenic substituted dinitrobenzenes with cysteine or cystine of skin proteins. J Exp Med, 1953, 98: 533-549.
– reference: 9) Matsumoto K, Mizukoshi K, Oyobikawa M, et al.: Objective evaluation of the efficacy of daily topical applications of cosmetics bases using the hairless mouse model of atopic dermatitis. Skin Res Technol, 2005, 11: 209-217.
– reference: 15) Kitchen SS, Partridge CH: A review of low intensity laser therapy: Parts I and II: Background, physiological effects and harzard, Physiotherapy, 1991, 77: 161-168.
– reference: 27) Chen WR, Carubelli R, Liu H, et al.: Detection of anti-tumor immunity induced by laser immunotherapy. Mol Biotechnol, 2003, 25: 45-52.
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Snippet [Purpose] The purpose of the present study was to investigate the effect of a magnetic infrared laser (MIL) on the 2, 4-dinitrofluorobenzene (DNFB)-induced...
「Abstract」. [Purpose] The purpose of the present study was to investigate the effect of a magnetic infrared laser (MIL) on the 2, 4-dinitrofluorobenzene...
Purpose: The purpose of the present study was to investigate the effect of a magnetic infrared laser (MIL) on the 2, 4-dinitrofluorobenzene (DNFB)-induced...
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SubjectTerms 2,4-dinitrofluorobenzene
Contact dermatitis
Magnetic infrared laser
Title Effects of Magnetic Infrared Laser on Dinitrofluorobenzene-induced Contact Dermatitis in Mice
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