Endophytic Trichoderma citrinoviride isolated from mountain-cultivated ginseng (Panax ginseng) has great potential as a biocontrol agent against ginseng pathogens

• Published in: Journal of ginseng research Vol. 43; no. 3; pp. 408 - 420
• Main Authors: Park, Young-Hwan, Chandra Mishra, Ratnesh, Yoon, Sunkyung, Kim, Hoki, Park, Changho, Seo, Sang-Tae, Bae, Hanhong
• Format: Journal Article
• Language: English
• Published: Korea (South) Elsevier B.V 01.07.2019; 고려인삼학회; Elsevier
• Subjects: Biocontrol agent; biological control; biological control agents; biosynthesis; Botrytis cinerea; elicitors; endophytes; Formulation; Fungal endophyte; Ginsenoside; ginsenosides; Iliyonectria radicicola; medicinal plants; metabolites; Mountain-cultivated ginseng; mycoparasites; Panax ginseng; Trichoderma citrinoviride; 기타의약학; Mountain-cultivated ginseng; Biocontrol agent; Fungal endophyte; Formulation; Ginsenoside
• ISSN: 1226-8453; 2093-4947
• DOI: 10.1016/j.jgr.2018.03.002

Ginseng (Panax ginseng Meyer) is an invaluable medicinal plant containing various bioactive metabolites (e.g., ginsenosides). Owing to its long cultivation period, ginseng is vulnerable to various biotic constraints. Biological control using endophytes is an important alternative to chemical control. In this study, endophytic Trichoderma citrinoviride PG87, isolated from mountain-cultivated ginseng, was evaluated for biocontrol activity against six major ginseng pathogens. T. citrinoviride exhibited antagonistic activity with mycoparasitism against all ginseng pathogens, with high endo-1,4-β-D-glucanase activity. T. citrinoviride inoculation significantly reduced the disease symptoms caused by Botrytis cinerea and Cylindrocarpon destructans and induced ginsenoside biosynthesis in ginseng plants. T. citrinoviride was formulated as dustable powder and granules. The formulated agents also exhibited significant biocontrol activity and induced ginsenosides production in the controlled environment and mountain area. Our results revealed that T. citrinoviride has great potential as a biological control agent and elicitor of ginsenoside production.