处理的牙本质基质对骨髓间充质干细胞成骨分化影响的研究
目的采用体外研究的方法评价处理的牙本质基质（TDM）对骨髓间充质干细胞（BMSCs）增殖及成骨分化的影响。方法将获取的牙本质颗粒进行梯度脱矿处理,制备TDM浸提液。分离培养人BMSCs后,将BMSCs培养于TDM浸提液中,CCK-8法检测细胞的增殖情况,培养7 d后提取细胞总蛋白采用Western blot检测成骨相关蛋白：Ⅰ型胶原蛋白（ColⅠ）、Runt相关转录因子-2（Runx2）的表达情况。结果 TDM浸提液培养后,与空白对照组及羟磷灰石/β-磷酸三钙组相比,细胞增殖明显;培养7 d后,TDM组的ColⅠ、Runx2蛋白的表达量明显增高。结论TDM可以促进BMSCs的增殖及成骨向分化,...
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| Published in | 华西口腔医学杂志 Vol. 34; no. 3; pp. 281 - 285 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
昆明医科大学口腔医学院，昆明 650031
2016
口腔再生医学国家地方联合工程实验室 四川大学，成都 610041%昆明医科大学口腔医学院,昆明,650031%口腔再生医学国家地方联合工程实验室 四川大学,成都,610041 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1000-1182 |
| DOI | 10.7518/hxkq.2016.03.013 |
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| Abstract | 目的采用体外研究的方法评价处理的牙本质基质（TDM）对骨髓间充质干细胞（BMSCs）增殖及成骨分化的影响。方法将获取的牙本质颗粒进行梯度脱矿处理,制备TDM浸提液。分离培养人BMSCs后,将BMSCs培养于TDM浸提液中,CCK-8法检测细胞的增殖情况,培养7 d后提取细胞总蛋白采用Western blot检测成骨相关蛋白：Ⅰ型胶原蛋白（ColⅠ）、Runt相关转录因子-2（Runx2）的表达情况。结果 TDM浸提液培养后,与空白对照组及羟磷灰石/β-磷酸三钙组相比,细胞增殖明显;培养7 d后,TDM组的ColⅠ、Runx2蛋白的表达量明显增高。结论TDM可以促进BMSCs的增殖及成骨向分化,提示其应用于骨组织工程的可行性。 |
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| AbstractList | Q 254; 目的：采用体外研究的方法评价处理的牙本质基质（TDM）对骨髓间充质干细胞（BMSCs）增殖及成骨分化的影响。方法将获取的牙本质颗粒进行梯度脱矿处理，制备TDM浸提液。分离培养人BMSCs后，将BMSCs培养于TDM浸提液中，CCK-8法检测细胞的增殖情况，培养7?d后提取细胞总蛋白采用Western?blot检测成骨相关蛋白：Ⅰ型胶原蛋白（ColⅠ）、Runt相关转录因子-2（Runx2）的表达情况。结果 TDM浸提液培养后，与空白对照组及羟磷灰石/β-磷酸三钙组相比，细胞增殖明显；培养7?d后，TDM组的ColⅠ、Runx2蛋白的表达量明显增高。结论 TDM可以促进BMSCs的增殖及成骨向分化，提示其应用于骨组织工程的可行性。 目的采用体外研究的方法评价处理的牙本质基质（TDM）对骨髓间充质干细胞（BMSCs）增殖及成骨分化的影响。方法将获取的牙本质颗粒进行梯度脱矿处理,制备TDM浸提液。分离培养人BMSCs后,将BMSCs培养于TDM浸提液中,CCK-8法检测细胞的增殖情况,培养7 d后提取细胞总蛋白采用Western blot检测成骨相关蛋白：Ⅰ型胶原蛋白（ColⅠ）、Runt相关转录因子-2（Runx2）的表达情况。结果 TDM浸提液培养后,与空白对照组及羟磷灰石/β-磷酸三钙组相比,细胞增殖明显;培养7 d后,TDM组的ColⅠ、Runx2蛋白的表达量明显增高。结论TDM可以促进BMSCs的增殖及成骨向分化,提示其应用于骨组织工程的可行性。 |
| Abstract_FL | Objective The effect of treated dentin matrix (TDM) to the proliferation and osteogenesis differentiation of bone marrow mesenchymal stem cells (BMSCs) is evaluated in vitro. Methods TDM leaching solution was prepared by dentine particles suffering from gradient demineralization. Human BMSCs were isolated and cultivated, and subsequently cultivated in the TDM leaching solution. The proliferation of BMSCs was detected by CCK-8. The osteogenesis-related proteins, including collagen typeⅠ(ColⅠ) and runt-related transcription factor-2 (Runx2), were extracted and detected by Western blot after a 7-day culture. Results Compared with the control group and hydroxyapatite (HA)/ β-tricalcium phosphate (β–TCP) group, the proliferation of BMSCs cultivated in TDM leaching solution was significantly improved. The expression of ColⅠ and Runx2 obviously increased after the 7-day cultivation in TDM leaching solution. Conclusion TDM can promote the proliferation and osteogenesis differentiation of BMSCs, implying the feasibility of the application in bone tissue engineering. |
| Author | 杨禾丰 胡瑜 孙晶晶 郭维华 田卫东 李松 |
| AuthorAffiliation | 昆明医科大学口腔医学院,昆明650031 口腔再生医学国家地方联合工程实验室四川大学,成都610041 |
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| Author_FL | Li Song Hu Yu Guo Weihua Sun Jingjing Tian Weidong Yang Hefeng |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DOI | 10.7518/hxkq.2016.03.013 |
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| DocumentTitleAlternate | Treated dentin matrix enhances proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells |
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| EndPage | 285 |
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| Keywords | 增殖 differentiation proliferation dentine 骨髓间充质干细胞 分化 bone marrow mesenchymal stem cells 牙本质 |
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| Notes | 51-1169/R Yang Hefeng,Hu Yu,Sun Jingjing,Guo Weihua,Tian Weidong,Li Song （1. The Affiliated Stomatology Hospital of Kunming Medical University, Kunming 650031, China; 2. National Engineering Laboratory for Oral Regenerative Medicine, Sichuan University, Chengdu 610041, China） bone marrow mesenchymal stem cells; proliferation; differentiation; dentine Objective The effect of treated dentin matrix（TDM） to the proliferation and osteogenesis differentiation of bone marrow mesenchymal stem cells（BMSCs） is evaluated in vitro. Methods TDM leaching solution was prepared by dentine particles suffering from gradient demineralization. Human BMSCs were isolated and cultivated, and subsequently cultivated in the TDM leaching solution. The proliferation of BMSCs was detected by CCK-8. The osteogenesis-related proteins, including collagen typeⅠ（ColⅠ） and runt-related transcription factor-2（Runx2）, were extracted and detected by Western blot after a 7-day culture. Results Compared with the control group and hydroxyapatite（HA）/ |
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| PublicationTitle | 华西口腔医学杂志 |
| PublicationTitleAlternate | West China Journal of Stomatology |
| PublicationYear | 2016 |
| Publisher | 昆明医科大学口腔医学院，昆明 650031 口腔再生医学国家地方联合工程实验室 四川大学，成都 610041%昆明医科大学口腔医学院,昆明,650031%口腔再生医学国家地方联合工程实验室 四川大学,成都,610041 |
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| Snippet | 目的采用体外研究的方法评价处理的牙本质基质（TDM）对骨髓间充质干细胞（BMSCs）增殖及成骨分化的影响。方法将获取的牙本质颗粒进行梯度脱矿处理,制备TDM浸提液。分离培养... Q 254; 目的：采用体外研究的方法评价处理的牙本质基质（TDM）对骨髓间充质干细胞（BMSCs）增殖及成骨分化的影响。方法将获取的牙本质颗粒进行梯度脱矿处理，制备TDM浸提... |
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| SubjectTerms | 分化 增殖 牙本质 骨髓间充质干细胞 |
| Title | 处理的牙本质基质对骨髓间充质干细胞成骨分化影响的研究 |
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