葎草花粉致敏蛋白多克隆抗体的制备及其在筛选致敏蛋白组分的应用
目的 制备、鉴定兔抗葎草花粉蛋白多克隆抗体，并初步应用于葎草花粉致敏蛋白组分的筛选。方法 用葎草花粉变应原浸液免疫兔，获得高效价抗葎草花粉蛋白抗血清，应用琼脂免疫双扩散、ELISA法以及Western blotting鉴定产生的抗体。同时对葎草花粉过敏性哮喘患者血清进行Western blotting检测。结果 兔抗血清效价经ELISA检测，效价〉1：10000，免疫双扩散结果表明抗血清只与葎草花粉变应原形成特异性IgG沉淀线，Western blotting检测兔抗血清IgG可以特异性识别葎草花粉8种蛋白组分，分子质量分别为100、88、76、69、65、55、49、38ku，患者血清IgG...
Saved in:
| Published in | Xi'an jiao tong da xue xue bao. Journal of Xi'an Jiaotong University (medical sciences). Yi xue ban Vol. 28; no. 2; pp. 161 - 163 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
西安交通大学医学院第二附属医院呼吸科,陕西西安,710004
2007
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 1671-8259 |
| DOI | 10.3969/j.issn.1671-8259.2007.02.012 |
Cover
| Abstract | 目的 制备、鉴定兔抗葎草花粉蛋白多克隆抗体，并初步应用于葎草花粉致敏蛋白组分的筛选。方法 用葎草花粉变应原浸液免疫兔，获得高效价抗葎草花粉蛋白抗血清，应用琼脂免疫双扩散、ELISA法以及Western blotting鉴定产生的抗体。同时对葎草花粉过敏性哮喘患者血清进行Western blotting检测。结果 兔抗血清效价经ELISA检测，效价〉1：10000，免疫双扩散结果表明抗血清只与葎草花粉变应原形成特异性IgG沉淀线，Western blotting检测兔抗血清IgG可以特异性识别葎草花粉8种蛋白组分，分子质量分别为100、88、76、69、65、55、49、38ku，患者血清IgG可以识别5种蛋白组分，分子质量分别为100、76、55、49、38ku。结论 制备的兔抗血清具有较高效价和较好的特异性，应用该血清筛选出的葎草花粉致敏蛋白组分与患者血清筛选的组分相似，因此可进一步应用于初筛cDNA表达文库。 |
|---|---|
| AbstractList | 目的 制备、鉴定兔抗葎草花粉蛋白多克隆抗体，并初步应用于葎草花粉致敏蛋白组分的筛选。方法 用葎草花粉变应原浸液免疫兔，获得高效价抗葎草花粉蛋白抗血清，应用琼脂免疫双扩散、ELISA法以及Western blotting鉴定产生的抗体。同时对葎草花粉过敏性哮喘患者血清进行Western blotting检测。结果 兔抗血清效价经ELISA检测，效价〉1：10000，免疫双扩散结果表明抗血清只与葎草花粉变应原形成特异性IgG沉淀线，Western blotting检测兔抗血清IgG可以特异性识别葎草花粉8种蛋白组分，分子质量分别为100、88、76、69、65、55、49、38ku，患者血清IgG可以识别5种蛋白组分，分子质量分别为100、76、55、49、38ku。结论 制备的兔抗血清具有较高效价和较好的特异性，应用该血清筛选出的葎草花粉致敏蛋白组分与患者血清筛选的组分相似，因此可进一步应用于初筛cDNA表达文库。 R562.2; 目的 制备、鉴定兔抗葎草花粉蛋白多克隆抗体,并初步应用于葎草花粉致敏蛋白组分的筛选.方法 用葎草花粉变应原浸液免疫兔,获得高效价抗葎草花粉蛋白抗血清,应用琼脂免疫双扩散、ELISA法以及Western blotting鉴定产生的抗体.同时对葎草花粉过敏性哮喘患者血清进行Western blotting检测.结果 兔抗血清效价经ELISA检测,效价＞1∶10000,免疫双扩散结果表明抗血清只与葎草花粉变应原形成特异性IgG沉淀线,Western blotting检测兔抗血清IgG可以特异性识别葎草花粉8种蛋白组分,分子质量分别为100、88、76、69、65、55、49、38ku,患者血清IgG可以识别5种蛋白组分,分子质量分别为100、76、55、49、38ku.结论 制备的兔抗血清具有较高效价和较好的特异性,应用该血清筛选出的葎草花粉致敏蛋白组分与患者血清筛选的组分相似,因此可进一步应用于初筛cDNA表达文库. |
| Author | 李雅莉 孙秀珍 高燕华 刘昀 张洁 冯向莉 |
| AuthorAffiliation | 西安交通大学医学院第二附属医院呼吸科,陕西西安710004 |
| AuthorAffiliation_xml | – name: 西安交通大学医学院第二附属医院呼吸科,陕西西安,710004 |
| Author_FL | Feng Xiangli Sun Xiuzhen Gao Yanhua Li Yali Liu Yun Zhang Jie |
| Author_FL_xml | – sequence: 1 fullname: Li Yali – sequence: 2 fullname: Sun Xiuzhen – sequence: 3 fullname: Gao Yanhua – sequence: 4 fullname: Liu Yun – sequence: 5 fullname: Zhang Jie – sequence: 6 fullname: Feng Xiangli |
| Author_xml | – sequence: 1 fullname: 李雅莉 孙秀珍 高燕华 刘昀 张洁 冯向莉 |
| BookMark | eNpV0DtPwlAUB_A7YCIiX8K4OLTeR7ltR0N8JSQuTC6kvQ8skBJtjLg5CEiJEiPGCI4MLA4aw6Dh23hp-RaWYEycTvLP75yTc9ZAyq_7AoBNBHViU3u7ontB4OuImkizcM7WMYSmDrEOEU6B9F--CrJB4LlJalGbUCMNjuP7u_i2E4dv0Xsnbn_MHnvxsBs9T9VooJrd-aA1C5--pw_R4FrdTNSorXqhak7Uyzh6Hc6v_rVEX4lpLeRnP-qP18GKdGqByP7WDCju7RbzB1rhaP8wv1PQGEVY49S0XGRIzm2MicQ5TgmTDEJkCdfhUNjc4jkoBcXENZhpusSUAmLOGRYGZSQDtpZjLxxfOn65VKmfn_nJwlLDuazyRsNdPAPi5OjEbiwtO6n75VMv0a7DqtKriRI2iG1QbJIfXbWEUA |
| ClassificationCodes | R562.2 |
| ContentType | Journal Article |
| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| Copyright_xml | – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| DBID | 2RA 92L CQIGP W91 ~WA 2B. 4A8 92I 93N PSX TCJ |
| DOI | 10.3969/j.issn.1671-8259.2007.02.012 |
| DatabaseName | 维普_期刊 中文科技期刊数据库-CALIS站点 维普中文期刊数据库 中文科技期刊数据库-医药卫生 中文科技期刊数据库- 镜像站点 Wanfang Data Journals - Hong Kong WANFANG Data Centre Wanfang Data Journals 万方数据期刊 - 香港版 China Online Journals (COJ) China Online Journals (COJ) |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| DocumentTitleAlternate | Preparation of anti-Humulus pollen protein polyclonal antibody and its application in determining the composition of Humulus pollen allergic protein |
| DocumentTitle_FL | Preparation of anti-Humulus pollen protein polyclonal antibody and its application in determining the composition of Humulus pollen allergic protein |
| EndPage | 163 |
| ExternalDocumentID | xaykdxxb200702012 24394627 |
| GrantInformation_xml | – fundername: 国家自然科学基金 funderid: (30371336) |
| GroupedDBID | --- -05 2RA 5XA 5XF 7X7 8FI 8FJ 92L ABDBF ABUWG AFKRA ALMA_UNASSIGNED_HOLDINGS BBAFP BENPR BPHCQ BVBZV BVXVI CCEZO CIEJG CQIGP EOJEC FYUFA OBODZ PQEST PQQKQ PQUKI PROAC U1G U5O UKHRP W91 ~WA 2B. 4A8 92I 93N ACUHS ALIPV CCPQU HMCUK PSX TCJ |
| ID | FETCH-LOGICAL-c612-d678b14fdd9223f25d63cfc0018ebad0e9d8d50fe623b4c77b37fe02ddc2e46c3 |
| ISSN | 1671-8259 |
| IngestDate | Thu May 29 03:54:44 EDT 2025 Fri Nov 25 07:20:43 EST 2022 |
| IsPeerReviewed | false |
| IsScholarly | true |
| Issue | 2 |
| Keywords | 葎草花粉 多克隆抗体 Western blotting |
| Language | Chinese |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c612-d678b14fdd9223f25d63cfc0018ebad0e9d8d50fe623b4c77b37fe02ddc2e46c3 |
| Notes | Humulus pollen R562.2 polyclonal antibody Western blotting Humulus pollen; polyclonal antibody; Western blotting 61-1399/R |
| PageCount | 3 |
| ParticipantIDs | wanfang_journals_xaykdxxb200702012 chongqing_backfile_24394627 |
| PublicationCentury | 2000 |
| PublicationDate | 2007 |
| PublicationDateYYYYMMDD | 2007-01-01 |
| PublicationDate_xml | – year: 2007 text: 2007 |
| PublicationDecade | 2000 |
| PublicationTitle | Xi'an jiao tong da xue xue bao. Journal of Xi'an Jiaotong University (medical sciences). Yi xue ban |
| PublicationTitleAlternate | Journal of Xi‘an Jiaotong University:Medical Sciences |
| PublicationTitle_FL | JOURNAL OF XI'AN JIAOTONG UNIVERSITY(MEDICAL SCIENCES) |
| PublicationYear | 2007 |
| Publisher | 西安交通大学医学院第二附属医院呼吸科,陕西西安,710004 |
| Publisher_xml | – name: 西安交通大学医学院第二附属医院呼吸科,陕西西安,710004 |
| SSID | ssib012869364 ssib038076391 ssib001051245 ssib022315977 ssib001104403 ssib002263349 ssib036440245 ssib002039773 ssib006705401 ssib012416230 ssj0001537995 ssib051375640 ssib052002713 |
| Score | 1.7230428 |
| Snippet | 目的 制备、鉴定兔抗葎草花粉蛋白多克隆抗体，并初步应用于葎草花粉致敏蛋白组分的筛选。方法 用葎草花粉变应原浸液免疫兔，获得高效价抗葎草花粉蛋白抗血清，应用琼脂免疫... R562.2; 目的 制备、鉴定兔抗葎草花粉蛋白多克隆抗体,并初步应用于葎草花粉致敏蛋白组分的筛选.方法 用葎草花粉变应原浸液免疫兔,获得高效价抗葎草花粉蛋白抗血清,应用琼脂... |
| SourceID | wanfang chongqing |
| SourceType | Aggregation Database Publisher |
| StartPage | 161 |
| SubjectTerms | blotting Western 多克隆抗体 葎草花粉 |
| Title | 葎草花粉致敏蛋白多克隆抗体的制备及其在筛选致敏蛋白组分的应用 |
| URI | http://lib.cqvip.com/qk/90540A/20072/24394627.html https://d.wanfangdata.com.cn/periodical/xaykdxxb200702012 |
| Volume | 28 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| journalDatabaseRights | – providerCode: PRVPQU databaseName: East & South Asia Database issn: 1671-8259 databaseCode: BVBZV dateStart: 20020101 customDbUrl: isFulltext: true dateEnd: 20210131 titleUrlDefault: https://search.proquest.com/eastsouthasia omitProxy: false ssIdentifier: ssj0001537995 providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Central issn: 1671-8259 databaseCode: BENPR dateStart: 20020101 customDbUrl: http://www.proquest.com/pqcentral?accountid=15518 isFulltext: true dateEnd: 20210131 titleUrlDefault: https://www.proquest.com/central omitProxy: true ssIdentifier: ssj0001537995 providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Health & Medical Collection issn: 1671-8259 databaseCode: 7X7 dateStart: 20020101 customDbUrl: isFulltext: true dateEnd: 20210131 titleUrlDefault: https://search.proquest.com/healthcomplete omitProxy: true ssIdentifier: ssj0001537995 providerName: ProQuest |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnR1Na9RANJQK4kUUFWtVinSOW5PJZDJzTHazFEFPFYqXsskkLRS2qC3UnjzY1rZoESti67GHXjwo0oPSf-N2t__C9ybT7LQVqcISJjPva94keR-bvHGc0aDltRijRU3h62qsYKwmCp7WVJErWSiv8BWmBh4-4uOP2YPJYHJgsG29tbQwn45lS3_8ruR_VhX6YF3xK9l_WNmKKHRAG9YXjrDCcDzXGpNEEOkRkWBDNIiQuhGR2CNJSGJ63ANtRhJOZEBEU2PFRMQIIyWJGyQJSMSIjLAhAj0k8VRwxAKCMiQJQ0jpaywYYhpYkJgbdOCSaPrimE45JOskEogVNZAvUBbuuQUDgLjPC-WxuMcgGNM9DFjYfjaSigCgqWVLNDuG8CBnKUA5WTiNtNggW8Sthp41UEiaJK4jaxSgjsL0YUIki0MlTJVS0RNq6GWRekKBnmtyAkRTgUmiXkIUCMmB7hp9EEmimEitOlAUqAhlQLo2FeCPIByP5W5VVRInrG64_1fH6TlLlDmiWuw6_ko6AFYOmYXxiEws9AorwOsVlIUNl8TCTB866TEFGVjSnhGb1rFQVLmXtDGmPPRqgpqK78baUmE9VahlOr2yKL_xwrzS7J028L7kUht4ZDBWMTClSOmYa97JP1lCfbH1YlYtLqYIBeERbkp-gWL-DuxfONl3vyHWAHfXKl_o4YbsljvrYrhku7_c9630BQ8xAKrsJdDyILpw--eCS7-fngDP3LPLP8IQcy3-uBcEBBAVvcDzw4D3azNhxTQaGne8rIrgY31HzDEda-aiM2rUdv9vSsPKMjNz7emn4C_rzxfbRas9bXnaE1ecyyZEHonK591VZ2Bp5przpPfube_NWm_9a_fbWm_1--GHzd7ORvfTQWd3u7O8cbS9crj-8dfB--72q87r_c7uamdzvbO83_m81_2yc_TyBEr3J8CsIOSPre7W3nVnoplM1MdrZl-YWgbxWE2Bf516rFBKggYLGijuZ0WG24vmaUu5uVRCBW6Rg-5TloVh6odF7lKlMpoznvk3nMH2XDu_6YwEYJcw5kyxXn-LMum2BE0DnjIlwIplQ85wpRYIK7JZLJY3RbGaAKfhkHPPKGrKGIXnU2cutVvnARp2LpX_GWFq97YzOP9sIb8Dwc58eldfob8Bu7kA4Q |
| linkProvider | ProQuest |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E8%91%8E%E8%8D%89%E8%8A%B1%E7%B2%89%E8%87%B4%E6%95%8F%E8%9B%8B%E7%99%BD%E5%A4%9A%E5%85%8B%E9%9A%86%E6%8A%97%E4%BD%93%E7%9A%84%E5%88%B6%E5%A4%87%E5%8F%8A%E5%85%B6%E5%9C%A8%E7%AD%9B%E9%80%89%E8%87%B4%E6%95%8F%E8%9B%8B%E7%99%BD%E7%BB%84%E5%88%86%E7%9A%84%E5%BA%94%E7%94%A8&rft.jtitle=%E8%A5%BF%E5%AE%89%E4%BA%A4%E9%80%9A%E5%A4%A7%E5%AD%A6%E5%AD%A6%E6%8A%A5%EF%BC%88%E5%8C%BB%E5%AD%A6%E7%89%88%EF%BC%89&rft.au=%E6%9D%8E%E9%9B%85%E8%8E%89&rft.au=%E5%AD%99%E7%A7%80%E7%8F%8D&rft.au=%E9%AB%98%E7%87%95%E5%8D%8E&rft.au=%E5%88%98%E6%98%80&rft.date=2007&rft.pub=%E8%A5%BF%E5%AE%89%E4%BA%A4%E9%80%9A%E5%A4%A7%E5%AD%A6%E5%8C%BB%E5%AD%A6%E9%99%A2%E7%AC%AC%E4%BA%8C%E9%99%84%E5%B1%9E%E5%8C%BB%E9%99%A2%E5%91%BC%E5%90%B8%E7%A7%91%2C%E9%99%95%E8%A5%BF%E8%A5%BF%E5%AE%89%2C710004&rft.issn=1671-8259&rft.volume=28&rft.issue=2&rft.spage=161&rft.epage=163&rft_id=info:doi/10.3969%2Fj.issn.1671-8259.2007.02.012&rft.externalDocID=xaykdxxb200702012 |
| thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fimage.cqvip.com%2Fvip1000%2Fqk%2F90540A%2F90540A.jpg http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Fxaykdxxb%2Fxaykdxxb.jpg |