去甲肾上腺素对胰腺癌细胞株MiaPaCa-2侵袭能力的影响

目的探讨经典神经递质去甲肾上腺素（norepinephrine,NE）对人胰腺癌细胞株MiaPaCa-2侵袭能力的影响及其作用机制。方法取人胰腺癌细胞株MiaPaCa-2为研究对象,实验分为空白对照组、0.1μmol/L NE干预组、1μmol/L NE干预组和10μmol/L NE干预组。干预48 h后,采用Transwell侵袭实验检测各组中MiaPaCa-2细胞侵袭能力的变化;RT-PCR法检测细胞中MMP-2、MMP-9和VEGF mRNA的表达;免疫细胞化学法检测细胞中MMP-2、MMP-9和VEGF蛋白的表达。结果1μmol/L NE干预组和10μmol/L NE干预组MiaPaC...

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Published inXi'an jiao tong da xue xue bao. Journal of Xi'an Jiaotong University (medical sciences). Yi xue ban Vol. 31; no. 3; pp. 265 - 268
Main Author 郭坤 沙焕臣 马清涌 王连才 胡恒通
Format Journal Article
LanguageChinese
Published 西安交通大学医学院第一附属医院肝胆外科,陕西西安,710061 2010
Subjects
去甲肾上腺素
神经递质
细胞侵袭
胰腺癌
胰腺癌
去甲肾上腺素
细胞侵袭
神经递质
Online AccessGet full text
ISSN1671-8259

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Abstract 目的探讨经典神经递质去甲肾上腺素（norepinephrine,NE）对人胰腺癌细胞株MiaPaCa-2侵袭能力的影响及其作用机制。方法取人胰腺癌细胞株MiaPaCa-2为研究对象,实验分为空白对照组、0.1μmol/L NE干预组、1μmol/L NE干预组和10μmol/L NE干预组。干预48 h后,采用Transwell侵袭实验检测各组中MiaPaCa-2细胞侵袭能力的变化;RT-PCR法检测细胞中MMP-2、MMP-9和VEGF mRNA的表达;免疫细胞化学法检测细胞中MMP-2、MMP-9和VEGF蛋白的表达。结果1μmol/L NE干预组和10μmol/L NE干预组MiaPaCa-2细胞的侵袭能力均高于空白对照组（P〈0.01）;1μmol/L NE干预组和10μmol/L NE干预组MiaPaCa-2细胞中MMP-2、MMP-9和VEGF mRNA和蛋白表达水平均高于空白对照组（P〈0.05）。结论NE可以通过上调MMP-2、MMP-9和VEGF的表达从而促进胰腺癌细胞发生远处侵袭转移。
AbstractList 目的探讨经典神经递质去甲肾上腺素（norepinephrine,NE）对人胰腺癌细胞株MiaPaCa-2侵袭能力的影响及其作用机制。方法取人胰腺癌细胞株MiaPaCa-2为研究对象,实验分为空白对照组、0.1μmol/L NE干预组、1μmol/L NE干预组和10μmol/L NE干预组。干预48 h后,采用Transwell侵袭实验检测各组中MiaPaCa-2细胞侵袭能力的变化;RT-PCR法检测细胞中MMP-2、MMP-9和VEGF mRNA的表达;免疫细胞化学法检测细胞中MMP-2、MMP-9和VEGF蛋白的表达。结果1μmol/L NE干预组和10μmol/L NE干预组MiaPaCa-2细胞的侵袭能力均高于空白对照组（P〈0.01）;1μmol/L NE干预组和10μmol/L NE干预组MiaPaCa-2细胞中MMP-2、MMP-9和VEGF mRNA和蛋白表达水平均高于空白对照组（P〈0.05）。结论NE可以通过上调MMP-2、MMP-9和VEGF的表达从而促进胰腺癌细胞发生远处侵袭转移。
R735.9; 目的 探讨经典神经递质去甲肾上腺素(norepinephrine, NE)对人胰腺癌细胞株MiaPaCa-2侵袭能力的影响及其作用机制.方法 取人胰腺癌细胞株MiaPaCa-2为研究对象,实验分为空白对照组、0.1μmol/L NE干预组、1μmol/L NE干预组和10μmol/L NE干预组.干预48h后,采用Transwell侵袭实验检测各组中MiaPaCa-2细胞侵袭能力的变化;RT-PCR法检测细胞中MMP-2、MMP-9和VEGF mRNA的表达;免疫细胞化学法检测细胞中MMP-2、MMP-9和VEGF蛋白的表达.结果 1μmol/L NE干预组和10μmol/L NE干预组MiaPaCa-2细胞的侵袭能力均高于空白对照组(P<0.01);1μmol/L NE干预组和10μmol/L NE干预组MiaPaCa-2细胞中MMP-2、MMP-9和VEGF mRNA和蛋白表达水平均高于空白对照组(P<0.05).结论 NE可以通过上调MMP-2、MMP-9和VEGF的表达从而促进胰腺癌细胞发生远处侵袭转移.
Author 郭坤 沙焕臣 马清涌 王连才 胡恒通
AuthorAffiliation 西安交通大学医学院第一附属医院肝胆外科,陕西西安710061
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DocumentTitleAlternate Effect of norepinephrine on the invasiveness of pancreatic cancer cell line MiaPaCa-2
DocumentTitle_FL Effect of norepinephrine on the invasiveness of pancreatic cancer cell line MiaPaCa-2
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Keywords 胰腺癌
去甲肾上腺素
细胞侵袭
神经递质
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pancreatic cancer
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PublicationTitle_FL JOURNAL OF XI'AN JIAOTONG UNIVERSITY(MEDICAL SCIENCES)
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Snippet 目的探讨经典神经递质去甲肾上腺素（norepinephrine,NE）对人胰腺癌细胞株MiaPaCa-2侵袭能力的影响及其作用机制。方法取人胰腺癌细胞株MiaPaCa-2为研究对象,实验分为空白对...
R735.9; 目的 探讨经典神经递质去甲肾上腺素(norepinephrine, NE)对人胰腺癌细胞株MiaPaCa-2侵袭能力的影响及其作用机制.方法 取人胰腺癌细胞株MiaPaCa-2为研究对象,实验...
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StartPage 265
SubjectTerms 去甲肾上腺素
神经递质
细胞侵袭
胰腺癌
Title 去甲肾上腺素对胰腺癌细胞株MiaPaCa-2侵袭能力的影响
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