Lipid transfer inhibitor protein (apolipoprotein F) concentration in normolipidemic and hyperlipidemic subjects

• Published in: Journal of lipid research Vol. 49; no. 1; pp. 127 - 135
• Main Authors: Morton, Richard E., Gnizak, Hannah M., Greene, Diane J., Cho, Kyung-Hyun, Paromov, Victor M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.2008; American Society for Biochemistry and Molecular Biology; Elsevier
• Subjects: Aged; Apolipoproteins - blood; Cholesterol Ester Transfer Proteins - blood; Cholesterol, HDL - blood; cholesteryl ester transfer protein; cholesteryl ester transfer protein inhibition; enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; Female; glycoprotein; Humans; Hypercholesterolemia - blood; Hypertriglyceridemia - blood; Male; Middle Aged; Recombinant Proteins; Triglycerides - blood; cholesteryl ester transfer protein inhibition; glycoprotein; cholesteryl ester transfer protein; enzyme-linked immunosorbent assay
• ISSN: 0022-2275; 1539-7262; 1539-7262
• DOI: 10.1194/jlr.M700258-JLR200

Lipid transfer inhibitor protein (LTIP) is an important regulator of cholesteryl ester transfer protein function. We report the development of an immunoassay for LTIP and its use to quantify LTIP in plasma of varying lipid contents. A rabbit antibody against bacterially produced recombinant LTIP detected two LTIP isoforms in plasma differing in carbohydrate content. This antibody was used in a competitive, enzyme-linked immunoassay that uses partially purified LTIP bound to microtiter plates. To optimize LTIP immunoreactivity, plasma samples required preincubation in 1% Tween-20 and 0.5% Nonidet P-40. In normolipidemic plasma, LTIP averaged 83.5 μg/ml. LTIP was 31% higher in males than in females. LTIP was positively associated with HDL cholesterol in normolipidemic males but not in females. In hypertriglyceridemic males, LTIP was only 56% of control values, whereas in hypertriglyceridemic females, LTIP tended to increase. Additionally, in males with normal cholesterol and triglyceride (TG) ⩽ 200 mg/dl, LTIP varied inversely with plasma TG. Overall, we have confirmed the negative association between plasma TG levels and LTIP previously suggested by a small data set, but now we demonstrate that this effect is seen only in males. The mechanisms underlying this gender-specific response to TG, and why LTIP and HDL levels correlate in males but not in females, remain to be determined.