高速逆流色谱分离制备川西獐牙菜中两种苷类化合物

采用高速逆流色谱从川西獐牙菜中分离制备了两种高纯度苷类化合物。以正丁醇-氯仿-甲醇-水（3.4∶8∶5∶6,v/v）为溶剂系统,主机转速为800 rpm,流速：0～210 min,1.5 mL/min;210～360 min,2.5 mL/min,检测波长254 nm的条件下进行分离制备,在360 min内从100 mg样品中一步分离制备得到1-O-樱草糖-3,7,8-三甲氧基口山酮（I,11 mg）和异荭草苷（II,24 mg）。经HPLC检测,两个化合物的纯度均在99%以上,结构由UV、1H和13C NMR鉴定。...

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Bibliographic Details
Published in天然产物研究与开发 Vol. 24; no. 6; pp. 800 - 803
Main Author 陈涛 贾静 王萍 尤进茂 刘永军 李玉林
Format Journal Article
LanguageChinese
Published 中国科学院研究生院,北京100049%中国科学院西北高原生物研究所,西宁,810001 2012
中国科学院西北高原生物研究所,西宁810001
Subjects
1-O-樱草糖-3,7,8-三甲氧基口山酮
川西獐牙菜
异荭草苷
高速逆流色谱
川西獐牙菜
1-O-樱草糖-3,7,8-三甲氧基(口山)酮
异荭草苷
高速逆流色谱
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ISSN1001-6880
DOI10.3969/j.issn.1001-6880.2012.06.020

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Summary:采用高速逆流色谱从川西獐牙菜中分离制备了两种高纯度苷类化合物。以正丁醇-氯仿-甲醇-水（3.4∶8∶5∶6,v/v）为溶剂系统,主机转速为800 rpm,流速：0～210 min,1.5 mL/min;210～360 min,2.5 mL/min,检测波长254 nm的条件下进行分离制备,在360 min内从100 mg样品中一步分离制备得到1-O-樱草糖-3,7,8-三甲氧基口山酮（I,11 mg）和异荭草苷（II,24 mg）。经HPLC检测,两个化合物的纯度均在99%以上,结构由UV、1H和13C NMR鉴定。
Bibliography:Swertia mussotti； HSCCC； 1-O-primever-osyl-3,7,8-trimethoxyxanthone； isoorientin
A high performance method for isolation and purification of two glycosides from Swertia mussotti was successfully established by utilizing high-speed counter-current chromatography（HSCCC）.Separation was performed with a two-phase solvent system composed of n-butanol-chloroform-methanol-water（3.4 ∶8 ∶5 ∶6,v/v）.1-O-primeverosyl-3,7,8-trimethoxyxanthone（11 mg） and isoorientin（24 mg） were isolated and purified in one-step elution from 100 mg crude sample under the conditions of a flow rate 1.5 mL/min in the initial 210 min followed by 2.5 mL/min,800 r/min and the detection wavelength of 254 nm.HPLC analysis showed that the purity of two compounds was over 99%.Their chemical structures were confirmed by UV,1H-and 13C NMR.
51-1335/Q
CHEN Tao1,2,JIA Jing1,2,WANG Ping1,2,YOU Jin-mao1,LIU Yong-jun1,LI Yu-lin1＊ 1Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810001,China; 2Graduate School of the Chinese Academy of S
ISSN:1001-6880
DOI:10.3969/j.issn.1001-6880.2012.06.020