中国扁蓿豆遗传多样性的ISSR分析

利用ISSR标记,对来源于中国7个省（市）的44个扁蓿豆居群的亲缘关系进行了分析。从80条ISSR引物中筛选出多态性强、重复性好的16条引物,对44个扁蓿豆居群基因组DNA进行扩增,共扩增出133条谱带,平均每个引物扩增出8.31条带,其中多态性带115条,多态性位点百分率为87.08%。扁蓿豆种质间遗传相似系数变化范围在0.436～0.908之间。利用UPGMA聚类分析,44个扁蓿豆居群被划分为8个类群,与地理来源有较高的相关性。...

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Published in植物遗传资源学报 Vol. 13; no. 1; pp. 48 - 51
Main Author 李志勇 李鸿雁 石凤翎 王小丽 赵成贵 师文贵 蔡丽艳
Format Journal Article
LanguageChinese
Published 中国农业科学院草原研究所/农业部沙尔沁牧草资源重点野外科学观测试验站,呼和浩特,010010%内蒙古农业大学生态环境学院,呼和浩特,010019%中国农业科学院郑州果树研究所,郑州,450009%内蒙古乌兰察布市凉城县畜牧局,凉城,013700 2012
Subjects
ISSR
亲缘关系
扁蓿豆
种质资源
扁蓿豆
亲缘关系
ISSR
种质资源
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ISSN1672-1810
DOI10.3969/j.issn.1672-1810.2012.01.008

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Summary:利用ISSR标记,对来源于中国7个省（市）的44个扁蓿豆居群的亲缘关系进行了分析。从80条ISSR引物中筛选出多态性强、重复性好的16条引物,对44个扁蓿豆居群基因组DNA进行扩增,共扩增出133条谱带,平均每个引物扩增出8.31条带,其中多态性带115条,多态性位点百分率为87.08%。扁蓿豆种质间遗传相似系数变化范围在0.436～0.908之间。利用UPGMA聚类分析,44个扁蓿豆居群被划分为8个类群,与地理来源有较高的相关性。
Bibliography:The genetic relationship of M.ruthenica populations from seven provinces of China were analyzed by ISSR marker.16 primers with strong polymorphism and good repeatability were screened from 80 ISSR primers.133 bands were amplified from 44 populations M.ruthenica genomic DNA,with an average of 8.31 bands in each primer,of which（115 bands）87.08% were polymorphic.The genetic dissimilarity coefficients of M.ruthenica germplasm ranged from 0.436 to 0.908.44 populations M.ruthenica germplasm were divided into eight groups with the higher correlation of geography resource by UPGMA cluster analysis.
Medicago ruthenica; Germplasm; Genetic relationship; ISSR
11-4996/S
LI Zhi-yong,LI Hong-yan,SHI Feng-ling,WANG Xiao-li,ZHAO Cheng-gui, SHI Wen-gui,CAI Li-yan（1Grassland Research Institute,Chinese Academy of Agriculture Science/SharaQinKey Wild Scientific Monitoring Station for Forage Resources of Ministry of Agriculture,Hohhot 010010;2College of Ecology and Environmental, Inner Mongolia Agriculture University,Huhhot 010019;3Z
ISSN:1672-1810
DOI:10.3969/j.issn.1672-1810.2012.01.008