茄子小孢子培养技术研究

【目的】开展茄子小孢子培养技术研究，为茄子单倍体育种在生产实践上应用提供技术支持。【方法】以6个不同基因型茄子栽培品种为试材进行小孢子培养，探究基因型、花药预处理、花药壁组织及供体植株生长环境对茄子小孢子培养的影响。【结果】6个茄子栽培品种均能诱导出愈伤组织，但诱导频率差异明显，其中，37号和128号品种的小孢子愈伤组织诱导频率极显著高于其他品种（P＜0.01，下同）；6个茄子品种中仅37号品种能形成再生植株，其中5株为单倍体植株，8株为双单倍体植株。茄子小孢子培养必需进行高温（36℃）热激处理，最佳处理时间6d；低温（4℃）预处理不能启动茄子小孢子脱分化形成愈伤组织，但能极大促进愈伤组织形成...

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Bibliographic Details
Published in南方农业学报 Vol. 48; no. 9; pp. 1641 - 1647
Main Author 范适;刘富中;陈钰辉;宋燕;刘志敏;张野;连勇
Format Journal Article
LanguageChinese
Published 湖南环境生物职业技术学院园林学院,湖南衡阳421005 2017
中国农业科学院蔬菜花卉研究所,北京100081%中国农业科学院蔬菜花卉研究所,北京,100081%湖南农业大学园林园艺学院,长沙,410128%广西农业科学院农业资源与环境研究所,南宁,530007
Subjects
茄子;小孢子培养;基因型;花药预处理;花药壁组织;供体植株生长环境
基因型
donor plant development environment
anther pretreatment
供体植株生长环境
茄子
花药预处理
anther wall tissue
小孢子培养
eggplant
microspore culture
花药壁组织
genotype
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ISSN2095-1191
DOI10.3969/j.issn.2095-1191.2017.09.17

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Summary:【目的】开展茄子小孢子培养技术研究，为茄子单倍体育种在生产实践上应用提供技术支持。【方法】以6个不同基因型茄子栽培品种为试材进行小孢子培养，探究基因型、花药预处理、花药壁组织及供体植株生长环境对茄子小孢子培养的影响。【结果】6个茄子栽培品种均能诱导出愈伤组织，但诱导频率差异明显，其中，37号和128号品种的小孢子愈伤组织诱导频率极显著高于其他品种（P＜0.01，下同）；6个茄子品种中仅37号品种能形成再生植株，其中5株为单倍体植株，8株为双单倍体植株。茄子小孢子培养必需进行高温（36℃）热激处理，最佳处理时间6d；低温（4℃）预处理不能启动茄子小孢子脱分化形成愈伤组织，但能极大促进愈伤组织形成；花药预处理以低温（4℃）处理6d+高温（36℃）处理6d的效果最佳，愈伤组织的诱导频率达60.0个/花药；花药壁组织与小孢子共培养能促进愈伤组织形成，最佳处理时间8~12d。供体植株在露地环境条件下小孢子愈伤组织诱导频率极显著高于温室环境条件。【结论】对露地环境条件下种植的37号和128号茄子的花药进行低温（4℃）处理6d+高温（36℃）处理6d，可获得最佳的小孢子愈伤组织诱导效果。
Bibliography:45-1381/S
eggplant;microspore culture;genotype;anther pretreatment;anther wall tissue;donor plant development environment
Objective】In this study,the technology of microspore culture of eggplant was studied to provide technical support for the application of haploid breeding of eggplant.【Method】Six eggplant varieties with various genotypes were used as experimental materials to conduct microspore culture.The effects of factors such as genotype,anther pretreatment,anther wall tissue and donor plant development environment on microspore culture were studied.【Result】Callus could be induced from the six cultivated varieties.But callus induction frequency was very different.Callus induction frequencies of varieties No.37and No.128were extremely significantly higher than those of other varieties(P<0.01,the same as below).Regenerated plants were obtained from variety No.37only,with five haploid plants and eight double haploid plants.High temperature(36℃)heat shock treatment was essential to microspore culture of eg
ISSN:2095-1191
DOI:10.3969/j.issn.2095-1191.2017.09.17