老芒麦和垂穗披碱草rDNA-ITS序列分析
分析了老芒麦和垂穗披碱草rDNA-ITS序列,为这两个种的鉴别提供分子指纹图谱,为系统发育提供分子生物学依据。结果表明,22份材料的ITS、ITS1、5.8S、ITS2序列长度均相同,依次为604bp、222bp、164bp、218bp,GC含量依次为62.25%～63.08%,62.16%～63.06%,59.76%,64.22%～65.60%。除5.8S外,碱基位点都有不同程度的变化,一些变异位点有明显的种性变异规律,可作为老芒麦和垂穗披碱草种质DNA指纹特异鉴别位点。7个ITS序列的同源性98.7%～99.8%,遗传分歧0.2～1.3,具有保守性,为非近期分化类群。系统发育分析表明不同来...
Saved in:
| Published in | 植物遗传资源学报 Vol. 13; no. 4; pp. 609 - 613 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
中国农业科学院研究生院,北京100081
2012
中国农业科学院草原研究所,呼和浩特010010 农业部草原资源与生态重点开放实验室,呼和浩特010010%中国农业科学院草原研究所,呼和浩特010010 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1672-1810 |
| DOI | 10.3969/j.issn.1672-1810.2012.04.016 |
Cover
| Abstract | 分析了老芒麦和垂穗披碱草rDNA-ITS序列,为这两个种的鉴别提供分子指纹图谱,为系统发育提供分子生物学依据。结果表明,22份材料的ITS、ITS1、5.8S、ITS2序列长度均相同,依次为604bp、222bp、164bp、218bp,GC含量依次为62.25%～63.08%,62.16%～63.06%,59.76%,64.22%～65.60%。除5.8S外,碱基位点都有不同程度的变化,一些变异位点有明显的种性变异规律,可作为老芒麦和垂穗披碱草种质DNA指纹特异鉴别位点。7个ITS序列的同源性98.7%～99.8%,遗传分歧0.2～1.3,具有保守性,为非近期分化类群。系统发育分析表明不同来源地的同种材料,差异主要表现在DNA变化快慢及碱基替换数,但相同的种归为一类,进一步反映了种内的遗传稳定性。 |
|---|---|
| AbstractList | S763.15; 分析了老芒麦和垂穗披碱草rDNA-ITS序列,为这两个种的鉴别提供分子指纹图谱,为系统发育提供分子生物学依据.结果表明,22份材料的ITS、ITS1、5.8S、ITS2序列长度均相同,依次为604bp、222bp、164bp、218bp,GC含量依次为62.25％～63.08％,62.16％～63.06％,59.76％,64.22％ ～ 65.60％.除5.8S外,碱基位点都有不同程度的变化,一些变异位点有明显的种性变异规律,可作为老芒麦和垂穗披碱草种质DNA指纹特异鉴别位点.7个ITS序列的同源性98.7％ ～99.8％,遗传分歧0.2～1.3,具有保守性,为非近期分化类群.系统发育分析表明不同来源地的同种材料,差异主要表现在DNA变化快慢及碱基替换数,但相同的种归为一类,进一步反映了种内的遗传稳定性. 分析了老芒麦和垂穗披碱草rDNA-ITS序列,为这两个种的鉴别提供分子指纹图谱,为系统发育提供分子生物学依据。结果表明,22份材料的ITS、ITS1、5.8S、ITS2序列长度均相同,依次为604bp、222bp、164bp、218bp,GC含量依次为62.25%～63.08%,62.16%～63.06%,59.76%,64.22%～65.60%。除5.8S外,碱基位点都有不同程度的变化,一些变异位点有明显的种性变异规律,可作为老芒麦和垂穗披碱草种质DNA指纹特异鉴别位点。7个ITS序列的同源性98.7%～99.8%,遗传分歧0.2～1.3,具有保守性,为非近期分化类群。系统发育分析表明不同来源地的同种材料,差异主要表现在DNA变化快慢及碱基替换数,但相同的种归为一类,进一步反映了种内的遗传稳定性。 |
| Author | 德英 穆怀彬 王照兰 赵来喜 刘新亮 |
| AuthorAffiliation | 中国农业科学院草原研究所,呼和浩特010010 农业部草原资源与生态重点开放实验室,呼和浩特010010 中国农业科学院研究生院,北京100081 |
| AuthorAffiliation_xml | – name: 中国农业科学院草原研究所,呼和浩特010010;农业部草原资源与生态重点开放实验室,呼和浩特010010%中国农业科学院草原研究所,呼和浩特010010;中国农业科学院研究生院,北京100081 |
| Author_FL | WANG Zhao-lan LIU Xin-liang DE Ying ZHAO Lai-xi MU Huai-bin |
| Author_FL_xml | – sequence: 1 fullname: DE Ying – sequence: 2 fullname: MU Huai-bin – sequence: 3 fullname: WANG Zhao-lan – sequence: 4 fullname: ZHAO Lai-xi – sequence: 5 fullname: LIU Xin-liang |
| Author_xml | – sequence: 1 fullname: 德英 穆怀彬 王照兰 赵来喜 刘新亮 |
| BookMark | eNo9jz1Lw1AYhe9QwVr7IwQXh6TvvTf3TTKW-lUoOpg93HzVFL3RBKntZB2kFkXp0q2DgyBOrv0_aeO_MFJxOvDwcA5ni1RUokJCdino3Ea70dPjLFM6RZNp1CopA8p0MHSgWCHVf75J6lkWewCMIbeQV0mjuBsVk-n34j2fPuXz-9XHbDn5XL19Fc-P6f5JU2s7Z_niJR_P8vHDcv66TTYieZGF9b-sEefwwGkda53To3ar2dF8BNR8iYYho3JHCJOLkKEd-VJ4MpAe0iAIDRMC5jEWgQmWsEzgNKSBhWhSjsLnNbK3ru1LFUnVdXvJTarKQXfYH_jDwa33-xCM8l_p7qxd_zxR3eu4tK_S-FKmA9dgKIALzn8ASZ9hQw |
| ClassificationCodes | S763.15 |
| ContentType | Journal Article |
| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| Copyright_xml | – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| DBID | 2RA 92L CQIGP W94 WU4 ~WA 2B. 4A8 92I 93N PSX TCJ |
| DOI | 10.3969/j.issn.1672-1810.2012.04.016 |
| DatabaseName | 中文科技期刊数据库 中文科技期刊数据库-CALIS站点 中文科技期刊数据库-7.0平台 中文科技期刊数据库-自然科学 中文科技期刊数据库-自然科学-生物科学 中文科技期刊数据库- 镜像站点 Wanfang Data Journals - Hong Kong WANFANG Data Centre Wanfang Data Journals 万方数据期刊 - 香港版 China Online Journals (COJ) China Online Journals (COJ) |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| DocumentTitleAlternate | rDNA-ITS sequence in Elymus sibiricus and E. nutans |
| DocumentTitle_FL | rDNA-ITS sequence in Elymus sibiricus and E. nutans |
| EndPage | 613 |
| ExternalDocumentID | zwyczyxb201204016 42650353 |
| GrantInformation_xml | – fundername: 中央级公益性科研院所基本科研业务费专项资金资助项目; "十二五"国家科技支撑课题 funderid: (2008-Z-1); (2011BAD17B01) |
| GroupedDBID | -04 2B. 2B~ 2C. 2RA 92E 92I 92L ACGFS ALMA_UNASSIGNED_HOLDINGS CCEZO CHDYS CQIGP CW9 FIJ IPNFZ RIG TCJ TGP U1G U5N W94 WU4 ~WA 4A8 93N ABJNI PSX |
| ID | FETCH-LOGICAL-c606-ca644af00255735e269fca5badab61dde470d2b22f0708587031e1d86671365c3 |
| ISSN | 1672-1810 |
| IngestDate | Thu May 29 03:55:42 EDT 2025 Wed Feb 14 10:45:42 EST 2024 |
| IsPeerReviewed | false |
| IsScholarly | false |
| Issue | 4 |
| Keywords | 系统发育 老芒麦 垂穗披碱草 ITS序列 DNA指纹鉴别 |
| Language | Chinese |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c606-ca644af00255735e269fca5badab61dde470d2b22f0708587031e1d86671365c3 |
| Notes | DE Ying1,2,MU Huai-bin1,2,WANG Zhao-lan1,2,ZHAO Lai-xi1,2,LIU Xin-liang1,3（1 Grassland Research Institute of Chinese Academy of Agricultural Sciences,Hohhot 010010;2Key Laboratory of Grassland Resources and Ecology,Ministry of Agriculture,Hohhot 010010;3Graduate School of Chinese Academy of Agricultural Sciences,Beijing 100081） Elymus sibiricus； E.nutans； ITS sequence； DNA fingerprint identification； Phylogeny Analysis of ITS sequences of Elymus sibiricus and E.nutans will provide useful information for germplasm DNA fingerprint identification and phylogeny.The results showed that the lengths of ITS,ITS1,5.8S and ITS2 in 22 accessions tested were identical,which were 604bp,222bp,164bp,and 218bp,and GC contents were 62.25%-63.08%,62.16%-63.06%,59.76%,and 64.22%-65.60%,respectively.Except for 5.8S,base sites varied to some extent.Some variable sites were species-specific,which can be used as DNA molecular markers of E.sibiricus and E.nutans.The identity of the seven ITS sequences ranged from 98.7% to 99.8%,and th |
| PageCount | 5 |
| ParticipantIDs | wanfang_journals_zwyczyxb201204016 chongqing_primary_42650353 |
| PublicationCentury | 2000 |
| PublicationDate | 2012 |
| PublicationDateYYYYMMDD | 2012-01-01 |
| PublicationDate_xml | – year: 2012 text: 2012 |
| PublicationDecade | 2010 |
| PublicationTitle | 植物遗传资源学报 |
| PublicationTitleAlternate | Journal of Plant Genetic Resources |
| PublicationTitle_FL | Journal of Plant Genetic Resources |
| PublicationYear | 2012 |
| Publisher | 中国农业科学院研究生院,北京100081 中国农业科学院草原研究所,呼和浩特010010 农业部草原资源与生态重点开放实验室,呼和浩特010010%中国农业科学院草原研究所,呼和浩特010010 |
| Publisher_xml | – name: 农业部草原资源与生态重点开放实验室,呼和浩特010010%中国农业科学院草原研究所,呼和浩特010010 – name: 中国农业科学院研究生院,北京100081 – name: 中国农业科学院草原研究所,呼和浩特010010 |
| SSID | ssib002263863 ssib051373152 ssib001101275 ssj0002507871 |
| Score | 1.5605164 |
| Snippet | 分析了老芒麦和垂穗披碱草rDNA-ITS序列,为这两个种的鉴别提供分子指纹图谱,为系统发育提供分子生物学依据。结果表明,22份材料的ITS、ITS1、5.8S、ITS2序列长度均相同,依次... S763.15; 分析了老芒麦和垂穗披碱草rDNA-ITS序列,为这两个种的鉴别提供分子指纹图谱,为系统发育提供分子生物学依据.结果表明,22份材料的ITS、ITS1、5.8S、ITS2序列长度均相... |
| SourceID | wanfang chongqing |
| SourceType | Aggregation Database Publisher |
| StartPage | 609 |
| SubjectTerms | DNA指纹鉴别 ITS序列 垂穗披碱草 系统发育 老芒麦 |
| Title | 老芒麦和垂穗披碱草rDNA-ITS序列分析 |
| URI | http://lib.cqvip.com/qk/84467A/201204/42650353.html https://d.wanfangdata.com.cn/periodical/zwyczyxb201204016 |
| Volume | 13 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| journalDatabaseRights | – providerCode: PRVALS databaseName: IngentaConnect Open Access Journals issn: 1672-1810 databaseCode: FIJ dateStart: 20100115 customDbUrl: isFulltext: true dateEnd: 20150115 titleUrlDefault: http://www.ingentaconnect.com/content/title?j_type=online&j_startat=Aa&j_endat=Af&j_pagesize=200&j_page=1 omitProxy: true ssIdentifier: ssj0002507871 providerName: Ingenta |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Pb9MwFLfKkBAXBALExh9NaD5N2RI7_ndM2kzbDrtQpN2qOE22UzdGJ1hPwAGNCQTaZbcdOCAhTlz3NfgM3cq34D0n7cKEJkBqo1f7veef81z72bKfCZlTzGqj_NzLrLQwQeHc00z5XiFCDAhoJXxxt8WaXH4arq6L9UbjR23X0m7fLmSDP54r-R-rQhrYFU_J_oNlJ0ohAWiwLzzBwvD8KxvTRONOBR04IqKG0cTQOKKRpInAn7rpiIRqyFI0MtQomkhkjmKXwmhcireoNjuttchbaT9BIVCjl5DQ2gk5QkuUBn3l7ZtjtxYToxB1gEptsBwAArhQMKRxk0Y-FhKDkhCZQbnxUWfUcmBLRGJsfld-QmPlkMUAcb6CXwLASjvxGMSbLk8nyIgEKC3hQrY_XxULHwQO7MK9EaBRrqyddnmAyndoAUlSXw0JakujgVTMA3_F_61v57U2HNY6aumb2pgvS76Lwwk30rjhBAtYmBSAGwKZC5AbXIji7fwC8HmEzwW_Qq4yBe4Pxh5dWT33UTGiWn1OyKALPI-BJgKueCDYZKEQfFToVN3CwRjANTJXoVu8DBuGC9nc6m08AyfInUnrFWlvo-Y-tW-SG9W8ZzYqG_Et0hhs3iaLo1evRweHP0--DA_fD4_fnH09Oj34dvb5--jDu3FTHJ58HO4fDfffnh5_ukPaS0m7uexVN3h4GUyMvSwFbzst3LxVcZEzaYosFTbtplYGMLCGyu8yy1gBA48WGu9SyIOullLh7suM3yVTva1efo_MWpUW3TQ3mnEbhkbCrBg05aKQRgW5FdNkZlLXznYZqKUztsQ0eVxVvlP9e593Bi_2ssHeS4vvCwayQM5cpuE-uY6M5drbAzLV39nNH4I32rePnHV_AfIWYD0 |
| linkProvider | Ingenta |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E8%80%81%E8%8A%92%E9%BA%A6%E5%92%8C%E5%9E%82%E7%A9%97%E6%8A%AB%E7%A2%B1%E8%8D%89rDNA-ITS%E5%BA%8F%E5%88%97%E5%88%86%E6%9E%90&rft.jtitle=%E6%A4%8D%E7%89%A9%E9%81%97%E4%BC%A0%E8%B5%84%E6%BA%90%E5%AD%A6%E6%8A%A5&rft.au=%E5%BE%B7%E8%8B%B1+%E7%A9%86%E6%80%80%E5%BD%AC+%E7%8E%8B%E7%85%A7%E5%85%B0+%E8%B5%B5%E6%9D%A5%E5%96%9C+%E5%88%98%E6%96%B0%E4%BA%AE&rft.date=2012&rft.issn=1672-1810&rft.volume=13&rft.issue=4&rft.spage=609&rft.epage=613&rft_id=info:doi/10.3969%2Fj.issn.1672-1810.2012.04.016&rft.externalDocID=42650353 |
| thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fimage.cqvip.com%2Fvip1000%2Fqk%2F84467A%2F84467A.jpg http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Fzwyczyxb%2Fzwyczyxb.jpg |