Increased concentrations of soluble B7-H3 and interleukin 36 in bronchoalveolar lavage fluid of Children with Mycoplasma pneumoniae pneumonia

• Published in: BMC infectious diseases Vol. 16; no. 1; p. 212
• Main Authors: Chen, Zhengrong, Zhao, Xin, Zhang, Xinxing, Zhang, Guangbo, Sun, Huiming, Jiang, Wujun, Wang, Yuqing, Zhu, Canhong, Ji, Wei, Yan, Yongdong
• Format: Journal Article
• Language: English
• Published: London BioMed Central 17.05.2016; BioMed Central Ltd
• Subjects: Adolescent; B cells; B7 Antigens - blood; Bacterial and fungal diseases; Bacterial pneumonia; Biomarkers - blood; Bronchoalveolar Lavage Fluid - chemistry; Bronchoalveolar Lavage Fluid - microbiology; Child; Child, Preschool; Diagnosis; Enzyme-Linked Immunosorbent Assay; Female; Health aspects; Humans; Infant; Infectious Diseases; Interleukin-1 - blood; Interleukins; Internal Medicine; Male; Medical Microbiology; Medicine; Medicine & Public Health; Mycoplasma pneumoniae - genetics; Mycoplasma pneumoniae - isolation & purification; Parasitology; Pneumonia; Pneumonia, Mycoplasma - blood; Pneumonia, Mycoplasma - cerebrospinal fluid; Pneumonia, Mycoplasma - diagnosis; Pneumonia, Mycoplasma - microbiology; Research Article; Tropical Medicine; United Kingdom; B7-H3; Bronchoalveolar lavage fluid; Interleukin 36; Children; pneumonia; Mycoplasma pneumoniae pneumonia
• ISSN: 1471-2334; 1471-2334
• DOI: 10.1186/s12879-016-1555-6

Background The purpose of this study is to explore the correlations of interleukin 36 (IL-36) and Soluble B7-H3 (sB7-H3) levels in bronchoalveolar lavage fluid (BALF) with clinical characteristics and laboratory findings. Methods A total of 35 children with M. pneumnoiae pneumonia (MPP) and 15 control subjects were enrolled. BALF concentrations of sB7-H3 and IL-36 were detected using enzyme-linked immunosorbent assays and clinical profiles of children with MPP were obtained. Results Children with MPP had significantly higher levels of sB7-H3 and IL-36 compared to control subjects (both P  < 0.05). Meanwhile, children with pleural effusion had significantly higher levels of sB7-H3 and IL-36 compared to children without pleural effusion (both P  < 0.05). BALF concentration of sB7-H3 was strongly associated with concentration of IL-36 ( r  = 0.796, P  < 0.0001) and sB7-H3 was correlated with duration of fever ( r  = 0.427, P  = 0.11) and length of stay ( r  = 0.345, P  = 0.043). Both concentrations of sB7-H3 and IL-36 were significantly decreased in convalescent phase after treatment (both P  < 0.05). Conclusion Both soluble B7-H3 and IL-36 may play an important role in pathogenesis of M. pneumoniae infection and sB7-H3 could be useful as a prognostic predictor or biomarker of MPP.