番茄抗叶霉病基因Cf-10和Cf-16的遗传分析及SSR标记
以Cf-10和Cf-16基因对东北三省分离鉴定的8个番茄叶霉病病原茵生理小种的抗性进行了评价，表明Cf-10和Cf-16基因对分化出的生理小种具有良好的抗性。分别对07881x08HN30（含Cf-10）和07880x08HN34（含Cf-16）构建的F1、F2代分离群体进行了遗传分析，并对F2群体进行了SSR标记。遗传分析表明，08HN30和08HN34的抗性均由一对显性单基因控制，从341对SSR引物中筛选出2个与Cf-10基因连锁的标记LEtaa001和LEaat003，遗传距离分别为9．7和22．9cM；1个与Cf-16基因连锁的标记Toml44—145，遗传距离为10．4cM。上述研...
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| Published in | 东北农业大学学报 Vol. 43; no. 1; pp. 88 - 92 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
湖北省农业科学院经济作物研究所,武汉,430064%东北农业大学园艺学院,哈尔滨,150030
2012
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1005-9369 |
| DOI | 10.3969/j.issn.1005-9369.2012.01.015 |
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| Abstract | 以Cf-10和Cf-16基因对东北三省分离鉴定的8个番茄叶霉病病原茵生理小种的抗性进行了评价，表明Cf-10和Cf-16基因对分化出的生理小种具有良好的抗性。分别对07881x08HN30（含Cf-10）和07880x08HN34（含Cf-16）构建的F1、F2代分离群体进行了遗传分析，并对F2群体进行了SSR标记。遗传分析表明，08HN30和08HN34的抗性均由一对显性单基因控制，从341对SSR引物中筛选出2个与Cf-10基因连锁的标记LEtaa001和LEaat003，遗传距离分别为9．7和22．9cM；1个与Cf-16基因连锁的标记Toml44—145，遗传距离为10．4cM。上述研究结果为抗源基因在番茄抗叶霉病育种中的应用及进一步精细定位Cf-10和Cf-16基因奠定了基础。 |
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| AbstractList | S641.2%S436.412; 以Cf-10和Cf-16基因对东北三省分离鉴定的8个番茄叶霉病病原菌生理小种的抗性进行了评价,表明Cf-10和Cf-16基因对分化出的生理小种具有良好的抗性.分别对07881 ×08HN30(含Cf-10)和07880×08HN34(含Cf-16)构建的F1、F2代分离群体进行了遗传分析,并对F2群体进行了SSR标记.遗传分析表明,08HN30和08HN34的抗性均由一对显性单基因控制,从341对SSR引物中筛选出2个与Cf-10基因连锁的标记LEtaa001和LEaat003,遗传距离分别为9.7和22.9 cM；1个与Cf-16基因连锁的标记Tom144-145,遗传距离为10.4 cM.上述研究结果为抗源基因在番茄抗叶霉病育种中的应用及进一步精细定位Cf-10和Cf-16基因奠定了基础. 以Cf-10和Cf-16基因对东北三省分离鉴定的8个番茄叶霉病病原茵生理小种的抗性进行了评价，表明Cf-10和Cf-16基因对分化出的生理小种具有良好的抗性。分别对07881x08HN30（含Cf-10）和07880x08HN34（含Cf-16）构建的F1、F2代分离群体进行了遗传分析，并对F2群体进行了SSR标记。遗传分析表明，08HN30和08HN34的抗性均由一对显性单基因控制，从341对SSR引物中筛选出2个与Cf-10基因连锁的标记LEtaa001和LEaat003，遗传距离分别为9．7和22．9cM；1个与Cf-16基因连锁的标记Toml44—145，遗传距离为10．4cM。上述研究结果为抗源基因在番茄抗叶霉病育种中的应用及进一步精细定位Cf-10和Cf-16基因奠定了基础。 |
| Author | 李宁 许向阳 姜景彬 李景富 |
| AuthorAffiliation | 湖北省农业科学院经济作物研究所,武汉430064 东北农业大学园艺学院,哈尔滨150030 |
| AuthorAffiliation_xml | – name: 湖北省农业科学院经济作物研究所,武汉,430064%东北农业大学园艺学院,哈尔滨,150030 |
| Author_FL | JIANG Jingbin XU Xiangyang LI Ning LI Jingfu |
| Author_FL_xml | – sequence: 1 fullname: LI Ning – sequence: 2 fullname: XU Xiangyang – sequence: 3 fullname: JIANG Jingbin – sequence: 4 fullname: LI Jingfu |
| Author_xml | – sequence: 1 fullname: 李宁 许向阳 姜景彬 李景富 |
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| ClassificationCodes | S641.2%S436.412 |
| ContentType | Journal Article |
| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DOI | 10.3969/j.issn.1005-9369.2012.01.015 |
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| DocumentTitleAlternate | Genetic analysis and SSR markers of leaf mold resistance gene Cf-10 and 0f-16 in tomato |
| DocumentTitle_FL | Genetic analysis and SSR markers of leaf mold resistance gene Cf-10 and Cf-16 in tomato |
| EndPage | 92 |
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| Keywords | Cf-16 番茄 Cf-10 SSR标记 叶霉病 |
| Language | Chinese |
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| Notes | LI Ning, XU Xiangyang, JIANG Jingbin, LI Jingfu （1. Cash Crop Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, China; 2. College of Horticulture, Northeast Agricultural University, Harbin 150030, China） tomato; Cf-10; Cf-16; leaf mould disease; SSR marker 23-1391/S Tomato Cf-10 and Cf-16 gene were resistant to eight prevalent physiological races of C. fulvum present in three northeastern provinces of China by virulence tests. FI and F2 populations were developed from 07881× 08HN30 and 07880 × 08HN34. The inheritance analysis indicated that leaf mould resistance gene Cf-10 and Cf-16 were single dominant genes, respectively. Total 341 pair of SSR primers were used to identify molecular markers linked to Cf-10 and Cf-16 gene, two polymorphic markers LEtaa001and LEaat003 were identified to be linked to Cf-10 in 08HN30 with the genetic distances of 9.7 and 22.9 cM, respectively, while a SSR marker Tom144-145 located on chromosome 11 were linked to Cf-16 resistant gene with the genetic distanc |
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| PublicationTitle | 东北农业大学学报 |
| PublicationTitleAlternate | Journal of Northeast Agricultural University |
| PublicationTitle_FL | Journal of Northeast Agricultural University |
| PublicationYear | 2012 |
| Publisher | 湖北省农业科学院经济作物研究所,武汉,430064%东北农业大学园艺学院,哈尔滨,150030 |
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| Snippet | 以Cf-10和Cf-16基因对东北三省分离鉴定的8个番茄叶霉病病原茵生理小种的抗性进行了评价，表明Cf-10和Cf-16基因对分化出的生理小种具有良好的抗性。分别对07881x08HN30（... S641.2%S436.412; 以Cf-10和Cf-16基因对东北三省分离鉴定的8个番茄叶霉病病原菌生理小种的抗性进行了评价,表明Cf-10和Cf-16基因对分化出的生理小种具有良好的抗性.分别... |
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| SubjectTerms | cf-10 Cf-16 sSR标记 叶霉病 番茄 |
| Title | 番茄抗叶霉病基因Cf-10和Cf-16的遗传分析及SSR标记 |
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