Dendritic Cells in Subcutaneous and Epicardial Adipose Tissue of Subjects with Type 2 Diabetes, Obesity, and Coronary Artery Disease

• Published in: Mediators of inflammation Vol. 2019; no. 2019; pp. 1 - 7
• Main Authors: Kotulák, Tomáš, Netuka, Ivan, Haluzík, Martin, Kopecký, Petr, Pořízka, Michal, Lipš, Michal, Kratochvílová, Helena, Lacinová, Zdeňka, Kloučková, Jana, Cinkajzlová, Anna, Mráz, Miloš, Lindner, Jaroslav
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2019; Hindawi; John Wiley & Sons, Inc; Wiley
• Subjects: Adipocytes; Adipose tissue; Adipose Tissue - immunology; Adipose Tissue - metabolism; Adipose tissues; Aged; Antigen-presenting cells; Blood glucose; Body fat; Cardiovascular disease; CD11c antigen; CD123 antigen; Coronary artery; Coronary Artery Disease - immunology; Coronary Artery Disease - metabolism; Coronary heart disease; Coronary vessels; Cytokines; Dendritic cells; Dendritic Cells - metabolism; Diabetes; Diabetes mellitus; Diabetes mellitus (non-insulin dependent); Diabetes Mellitus, Type 2 - immunology; Diabetes Mellitus, Type 2 - metabolism; Ethylenediaminetetraacetic acid; Female; Flow cytometry; Heart diseases; Heart surgery; Hemoglobin; Histocompatibility antigen HLA; Humans; Immune response; Immunology; Inflammation; Insulin resistance; Laboratories; Male; Metabolism; Middle Aged; Multiple regression analysis; Obesity; Obesity - immunology; Obesity - metabolism; Pericardium - immunology; Pericardium - metabolism; Peripheral blood; Subcutaneous Fat - immunology; Subcutaneous Fat - metabolism; Type 2 diabetes; Czech Republic; United States > US
• ISSN: 0962-9351; 1466-1861; 1466-1861
• DOI: 10.1155/2019/5481725

Dendritic cells (DCs) are professional antigen-presenting cells contributing to regulation of lymphocyte immune response. DCs are divided into two subtypes: CD11c-positive conventional or myeloid (cDCs) and CD123-positive plasmacytoid (pDCs) DCs. The aim of the study was to assess DCs (HLA-DR+ lineage-) and their subtypes by flow cytometry in peripheral blood and subcutaneous (SAT) and epicardial (EAT) adipose tissue in subjects with (T2DM, n=12) and without (non-T2DM, n=17) type 2 diabetes mellitus undergoing elective cardiac surgery. Subjects with T2DM had higher fasting glycemia (8.6±0.7 vs. 5.8±0.2 mmol/l, p<0.001) and glycated hemoglobin (52.0±3.4 vs. 36.9±1.0 mmol/mol, p<0.001) and tended to have more pronounced inflammation (hsCRP: 9.8±3.1 vs. 5.1±1.9 mg/ml, p=0.177) compared with subjects without T2DM. T2DM was associated with reduced total DCs in SAT (1.57±0.65 vs. 4.45±1.56% for T2DM vs. non-T2DM, p=0.041) with a similar, albeit insignificant, trend in EAT (0.996±0.33 vs. 2.46±0.78% for T2DM vs. non-T2DM, p=0.171). When analyzing DC subsets, no difference in cDCs was seen between any of the studied groups or adipose tissue pools. In contrast, pDCs were increased in both SAT (13.5±2.0 vs. 4.6±1.9% of DC cells, p=0.005) and EAT (29.1±8.7 vs. 8.4±2.4% of DC, p=0.045) of T2DM relative to non-T2DM subjects as well as in EAT of the T2DM group compared with corresponding SAT (29.1±8.7 vs. 13.5±2.0% of DC, p=0.020). Neither obesity nor coronary artery disease (CAD) significantly influenced the number of total, cDC, or pDC in SAT or EAT according to multiple regression analysis. In summary, T2DM decreased the amount of total dendritic cells in subcutaneous adipose tissue and increased plasmacytoid dendritic cells in subcutaneous and even more in epicardial adipose tissue. These findings suggest a potential role of pDCs in the development of T2DM-associated adipose tissue low-grade inflammation.