Bioluminescent-based imaging and quantification of glucose uptake in vivo

Glucose is a major source of energy for most living organisms, and its aberrant uptake is linked to many pathological conditions. However, our understanding of disease-associated glucose flux is limited owing to the lack of robust tools. To date, positron-emission tomography imaging remains the gold...

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Published inNature methods Vol. 16; no. 6; pp. 526 - 532
Main Authors Maric, Tamara, Mikhaylov, Georgy, Khodakivskyi, Pavlo, Bazhin, Arkadiy, Sinisi, Riccardo, Bonhoure, Nicolas, Yevtodiyenko, Aleksey, Jones, Anthony, Muhunthan, Vishaka, Abdelhady, Gihad, Shackelford, David, Goun, Elena
Format Journal Article
LanguageEnglish
Published New York Nature Publishing Group US 01.06.2019
Nature Publishing Group
Subjects
631/1647/245/2222
631/67/2321
631/67/2327
631/92/96
Absorption
Animals
Bioinformatics
Biological Microscopy
Biological Techniques
Biological Transport
Bioluminescence
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Disease control
Drug development
Drug metabolism
Emission
Female
Fluorodeoxyglucose F18 - metabolism
Glucose
Glucose - metabolism
Glucose transport
Glucose Transporter Type 1 - physiology
Humans
In vivo methods and tests
Life Sciences
Luciferases - metabolism
Medical imaging
Medical research
Medicine, Experimental
Metabolism
Metabolites
Mice, Knockout
Mice, Nude
Molecular probes
Neoplasms, Experimental - diagnostic imaging
Neoplasms, Experimental - metabolism
Neoplasms, Experimental - pathology
Positron emission tomography
Positron-Emission Tomography - methods
Proteomics
Radiopharmaceuticals - metabolism
Reagents
Testing
Tomography
Tracers
Tumor Cells, Cultured
Online AccessGet full text
ISSN1548-7091
1548-7105
1548-7105
DOI10.1038/s41592-019-0421-z

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Abstract Glucose is a major source of energy for most living organisms, and its aberrant uptake is linked to many pathological conditions. However, our understanding of disease-associated glucose flux is limited owing to the lack of robust tools. To date, positron-emission tomography imaging remains the gold standard for measuring glucose uptake, and no optical tools exist for non-invasive longitudinal imaging of this important metabolite in in vivo settings. Here, we report the development of a bioluminescent glucose-uptake probe for real-time, non-invasive longitudinal imaging of glucose absorption both in vitro and in vivo. In addition, we demonstrate that the sensitivity of our method is comparable with that of commonly used 18 F-FDG-positron-emission-tomography tracers and validate the bioluminescent glucose-uptake probe as a tool for the identification of new glucose transport inhibitors. The new imaging reagent enables a wide range of applications in the fields of metabolism and drug development. A bioluminescent glucose-uptake probe enables accurate, real-time, non-invasive longitudinal imaging of d -glucose absorption both in vitro and in vivo.
AbstractList Glucose is a major source of energy for most living organisms and its aberrant uptake is linked to many pathological conditions. However, our understanding of disease-associated glucose flux is limited due to the lack of robust tools. To date, positron emission tomography (PET) imaging remains the gold standard for measuring glucose uptake, and no optical tools exist for non-invasive longitudinal imaging of this important metabolite in in vivo settings. Here we report the development of a novel bioluminescent glucose uptake probe (BiGluc) for real-time, non-invasive longitudinal imaging of glucose absorption both in vitro and in vivo . In addition, we demonstrate that the sensitivity of our method is comparable with commonly used 18 F-FDG-PET tracers and validate BiGluc as a tool for the identification of novel glucose transport inhibitors. The new imaging reagent enables a wide range of applications in the field of metabolism and drug development.
Glucose is a major source of energy for most living organisms, and its aberrant uptake is linked to many pathological conditions. However, our understanding of disease-associated glucose flux is limited owing to the lack of robust tools. To date, positron-emission tomography imaging remains the gold standard for measuring glucose uptake, and no optical tools exist for non-invasive longitudinal imaging of this important metabolite in in vivo settings. Here, we report the development of a bioluminescent glucose-uptake probe for real-time, non-invasive longitudinal imaging of glucose absorption both in vitro and in vivo. In addition, we demonstrate that the sensitivity of our method is comparable with that of commonly used 18F-FDG-positron-emission-tomography tracers and validate the bioluminescent glucose-uptake probe as a tool for the identification of new glucose transport inhibitors. The new imaging reagent enables a wide range of applications in the fields of metabolism and drug development.A bioluminescent glucose-uptake probe enables accurate, real-time, non-invasive longitudinal imaging of d-glucose absorption both in vitro and in vivo.
Glucose is a major source of energy for most living organisms, and its aberrant uptake is linked to many pathological conditions. However, our understanding of disease-associated glucose flux is limited owing to the lack of robust tools. To date, positron-emission tomography imaging remains the gold standard for measuring glucose uptake, and no optical tools exist for non-invasive longitudinal imaging of this important metabolite in in vivo settings. Here, we report the development of a bioluminescent glucose-uptake probe for real-time, non-invasive longitudinal imaging of glucose absorption both in vitro and in vivo. In addition, we demonstrate that the sensitivity of our method is comparable with that of commonly used F-FDG-positron-emission-tomography tracers and validate the bioluminescent glucose-uptake probe as a tool for the identification of new glucose transport inhibitors. The new imaging reagent enables a wide range of applications in the fields of metabolism and drug development.
Glucose is a major source of energy for most living organisms, and its aberrant uptake is linked to many pathological conditions. However, our understanding of disease-associated glucose flux is limited owing to the lack of robust tools. To date, positron-emission tomography imaging remains the gold standard for measuring glucose uptake, and no optical tools exist for non-invasive longitudinal imaging of this important metabolite in in vivo settings. Here, we report the development of a bioluminescent glucose-uptake probe for real-time, non-invasive longitudinal imaging of glucose absorption both in vitro and in vivo. In addition, we demonstrate that the sensitivity of our method is comparable with that of commonly used .sup.18F-FDG-positron-emission-tomography tracers and validate the bioluminescent glucose-uptake probe as a tool for the identification of new glucose transport inhibitors. The new imaging reagent enables a wide range of applications in the fields of metabolism and drug development. A bioluminescent glucose-uptake probe enables accurate, real-time, non-invasive longitudinal imaging of d-glucose absorption both in vitro and in vivo.
Glucose is a major source of energy for most living organisms, and its aberrant uptake is linked to many pathological conditions. However, our understanding of disease-associated glucose flux is limited owing to the lack of robust tools. To date, positron-emission tomography imaging remains the gold standard for measuring glucose uptake, and no optical tools exist for non-invasive longitudinal imaging of this important metabolite in in vivo settings. Here, we report the development of a bioluminescent glucose-uptake probe for real-time, non-invasive longitudinal imaging of glucose absorption both in vitro and in vivo. In addition, we demonstrate that the sensitivity of our method is comparable with that of commonly used 18F-FDG-positron-emission-tomography tracers and validate the bioluminescent glucose-uptake probe as a tool for the identification of new glucose transport inhibitors. The new imaging reagent enables a wide range of applications in the fields of metabolism and drug development.Glucose is a major source of energy for most living organisms, and its aberrant uptake is linked to many pathological conditions. However, our understanding of disease-associated glucose flux is limited owing to the lack of robust tools. To date, positron-emission tomography imaging remains the gold standard for measuring glucose uptake, and no optical tools exist for non-invasive longitudinal imaging of this important metabolite in in vivo settings. Here, we report the development of a bioluminescent glucose-uptake probe for real-time, non-invasive longitudinal imaging of glucose absorption both in vitro and in vivo. In addition, we demonstrate that the sensitivity of our method is comparable with that of commonly used 18F-FDG-positron-emission-tomography tracers and validate the bioluminescent glucose-uptake probe as a tool for the identification of new glucose transport inhibitors. The new imaging reagent enables a wide range of applications in the fields of metabolism and drug development.
Glucose is a major source of energy for most living organisms, and its aberrant uptake is linked to many pathological conditions. However, our understanding of disease-associated glucose flux is limited owing to the lack of robust tools. To date, positron-emission tomography imaging remains the gold standard for measuring glucose uptake, and no optical tools exist for non-invasive longitudinal imaging of this important metabolite in in vivo settings. Here, we report the development of a bioluminescent glucose-uptake probe for real-time, non-invasive longitudinal imaging of glucose absorption both in vitro and in vivo. In addition, we demonstrate that the sensitivity of our method is comparable with that of commonly used .sup.18F-FDG-positron-emission-tomography tracers and validate the bioluminescent glucose-uptake probe as a tool for the identification of new glucose transport inhibitors. The new imaging reagent enables a wide range of applications in the fields of metabolism and drug development.
Glucose is a major source of energy for most living organisms, and its aberrant uptake is linked to many pathological conditions. However, our understanding of disease-associated glucose flux is limited owing to the lack of robust tools. To date, positron-emission tomography imaging remains the gold standard for measuring glucose uptake, and no optical tools exist for non-invasive longitudinal imaging of this important metabolite in in vivo settings. Here, we report the development of a bioluminescent glucose-uptake probe for real-time, non-invasive longitudinal imaging of glucose absorption both in vitro and in vivo. In addition, we demonstrate that the sensitivity of our method is comparable with that of commonly used 18 F-FDG-positron-emission-tomography tracers and validate the bioluminescent glucose-uptake probe as a tool for the identification of new glucose transport inhibitors. The new imaging reagent enables a wide range of applications in the fields of metabolism and drug development. A bioluminescent glucose-uptake probe enables accurate, real-time, non-invasive longitudinal imaging of d -glucose absorption both in vitro and in vivo.
Audience Academic
Author Jones, Anthony
Bonhoure, Nicolas
Mikhaylov, Georgy
Sinisi, Riccardo
Goun, Elena
Abdelhady, Gihad
Khodakivskyi, Pavlo
Yevtodiyenko, Aleksey
Shackelford, David
Muhunthan, Vishaka
Bazhin, Arkadiy
Maric, Tamara
AuthorAffiliation 3 Department of Pulmonary and Critical Care Medicine, David Geffen School of Medicine, University of California, Los Angeles, California, USA
1 Institute of Chemical Sciences and Engineering (ISIC), Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland
2 Nestlé Institute of Health Sciences SA, EPFL Innovation Park, Bâtiments G/H, Lausanne, Switzerland
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– name: 1 Institute of Chemical Sciences and Engineering (ISIC), Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland
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  organization: Department of Pulmonary and Critical Care Medicine, David Geffen School of Medicine, University of California
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  surname: Muhunthan
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  orcidid: 0000-0002-4214-0656
  surname: Goun
  fullname: Goun, Elena
  email: elena.goun@epfl.ch
  organization: Institute of Chemical Sciences and Engineering (ISIC), Swiss Federal Institute of Technology (EPFL)
BackLink https://www.ncbi.nlm.nih.gov/pubmed/31086341$$D View this record in MEDLINE/PubMed
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ContentType Journal Article
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Snippet Glucose is a major source of energy for most living organisms, and its aberrant uptake is linked to many pathological conditions. However, our understanding of...
Glucose is a major source of energy for most living organisms and its aberrant uptake is linked to many pathological conditions. However, our understanding of...
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SubjectTerms 631/1647/245/2222
631/67/2321
631/67/2327
631/92/96
Absorption
Animals
Bioinformatics
Biological Microscopy
Biological Techniques
Biological Transport
Bioluminescence
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Disease control
Drug development
Drug metabolism
Emission
Female
Fluorodeoxyglucose F18 - metabolism
Glucose
Glucose - metabolism
Glucose transport
Glucose Transporter Type 1 - physiology
Humans
In vivo methods and tests
Life Sciences
Luciferases - metabolism
Medical imaging
Medical research
Medicine, Experimental
Metabolism
Metabolites
Mice, Knockout
Mice, Nude
Molecular probes
Neoplasms, Experimental - diagnostic imaging
Neoplasms, Experimental - metabolism
Neoplasms, Experimental - pathology
Positron emission tomography
Positron-Emission Tomography - methods
Proteomics
Radiopharmaceuticals - metabolism
Reagents
Testing
Tomography
Tracers
Tumor Cells, Cultured
Title Bioluminescent-based imaging and quantification of glucose uptake in vivo
URI https://link.springer.com/article/10.1038/s41592-019-0421-z
https://www.ncbi.nlm.nih.gov/pubmed/31086341
https://www.proquest.com/docview/2232682214
https://www.proquest.com/docview/2232014513
https://pubmed.ncbi.nlm.nih.gov/PMC6546603
Volume 16
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