Parvimonas micra can translocate from the subgingival sulcus of the human oral cavity to colorectal adenocarcinoma

• Published in: Molecular oncology Vol. 18; no. 5; pp. 1143 - 1173
• Main Authors: Conde‐Pérez, Kelly, Buetas, Elena, Aja‐Macaya, Pablo, Martin‐De Arribas, Elsa, Iglesias‐Corrás, Iago, Trigo‐Tasende, Noelia, Nasser‐Ali, Mohammed, Estévez, Lara S., Rumbo‐Feal, Soraya, Otero‐Alén, Begoña, Noguera, Jose F., Concha, Ángel, Pardiñas‐López, Simón, Carda‐Diéguez, Miguel, Gómez‐Randulfe, Igor, Martínez‐Lago, Nieves, Ladra, Susana, Aparicio, Luis A., Bou, Germán, Mira, Alex, Vallejo, Juan A., Poza, Margarita
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01.05.2024; John Wiley and Sons Inc; Wiley
• Subjects: Adenocarcinoma; Adenocarcinoma - microbiology; Adenocarcinoma - pathology; Aged; Antibiotics; Bacteria; Biomarkers; Cancer therapies; Colorectal cancer; Colorectal carcinoma; Colorectal Neoplasms - microbiology; Colorectal Neoplasms - pathology; Comparative analysis; CRISPR; Development and progression; DNA methylation; Feces; Feces - microbiology; Female; Firmicutes; Genomes; Genomics; Gingiva; Gingiva - microbiology; Gingiva - pathology; Gum disease; Hospitals; Humans; Immune system; Immunology; Inflammation; Male; Medical prognosis; metabarcoding; Metastasis; metatranscriptomics; microbiome; Microbiomes; Microbiota; Middle Aged; Mouth - microbiology; Oral cavity; Parvimonas micra; Pathogens; Peptostreptococcus - genetics; Peptostreptococcus - isolation & purification; periodontal disease; Phreatodrobia micra; RNA; RNA sequencing; RNA, Ribosomal, 16S - genetics; Saliva; Saliva - microbiology; Scientific equipment and supplies industry; Tumors; United States; United Kingdom; Spain; United States > US; Parvimonas micra; metabarcoding; colorectal cancer; metatranscriptomics; microbiome; periodontal disease
• ISSN: 1574-7891; 1878-0261; 1878-0261
• DOI: 10.1002/1878-0261.13506

Oral and intestinal samples from a cohort of 93 colorectal cancer (CRC) patients and 30 healthy controls (non‐CRC) were collected for microbiome analysis. Saliva (28 non‐CRC and 94 CRC), feces (30 non‐CRC and 97 CRC), subgingival fluid (20 CRC), and tumor tissue samples (20 CRC) were used for 16S metabarcoding and/or RNA sequencing (RNAseq) approaches. A differential analysis of the abundance, performed with the ANCOM‐BC package, adjusting the P‐values by the Holm‐Bonferroni method, revealed that Parvimonas was significantly over‐represented in feces from CRC patients (P‐value < 0.001) compared to healthy controls. A total of 11 Parvimonas micra isolates were obtained from the oral cavity and adenocarcinoma of CRC patients. Genome analysis identified a pair of isolates from the same patient that shared 99.2% identity, demonstrating that P. micra can translocate from the subgingival cavity to the gut. The data suggest that P. micra could migrate in a synergistic consortium with other periodontal bacteria. Metatranscriptomics confirmed that oral bacteria were more active in tumor than in non‐neoplastic tissues. We suggest that P. micra could be considered as a CRC biomarker detected in non‐invasive samples such as feces. Parvimonas micra can translocate from the oral cavity to the intestinal lumen, through the circulatory or gastrointestinal system, where the anaerobic bacteria undergoes some genome and transcriptomic changes needed for the adaptation to its new niche. P. micra can be considered as an excellent CRC biomarker detected in non‐invasive samples.