干预胰岛素样生长因子Ⅰ型受体活化联合抗癌药物抑制肝癌细胞增殖的协同作用分析
目的探讨干预胰岛素样生长因子Ⅰ型受体（IGF-ⅠR）基因转录,对抑制肝癌细胞增殖药物的协同作用。方法以p GPU6/GFP/Neo-IGF-ⅠR-shRNA高效质粒,转染IGF-ⅠR高表达的HBV阳性肝癌细胞PLC/PRF/5和HBV阴性Bel-7404,设空白对照组、阴性对照组和IGF-ⅠR-shRNA干预组;以荧光定量逆转录PCR和Western Blot分析RNA和蛋白表达;以细胞计数试剂盒分析细胞增殖;以流式细胞术、Annexin-V-PE/7-ADD分析细胞周期与凋亡。计量资料组间比较采用t检验,计数资料组间比较采用Fisher确切概率法。结果 IGF-ⅠR shRNA转染肝癌细胞P...
Saved in:
| Published in | 临床肝胆病杂志 Vol. 32; no. 8; pp. 1543 - 1548 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
南通大学附属医院临床医学研究中心,江苏南通,226001
2016
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 1001-5256 |
| DOI | 10.3969/j.issn.1001-5256.2016.08.022 |
Cover
| Abstract | 目的探讨干预胰岛素样生长因子Ⅰ型受体（IGF-ⅠR）基因转录,对抑制肝癌细胞增殖药物的协同作用。方法以p GPU6/GFP/Neo-IGF-ⅠR-shRNA高效质粒,转染IGF-ⅠR高表达的HBV阳性肝癌细胞PLC/PRF/5和HBV阴性Bel-7404,设空白对照组、阴性对照组和IGF-ⅠR-shRNA干预组;以荧光定量逆转录PCR和Western Blot分析RNA和蛋白表达;以细胞计数试剂盒分析细胞增殖;以流式细胞术、Annexin-V-PE/7-ADD分析细胞周期与凋亡。计量资料组间比较采用t检验,计数资料组间比较采用Fisher确切概率法。结果 IGF-ⅠR shRNA转染肝癌细胞PLC/PRF/5效率为71%、肝癌细胞Bel-7404为90%,2种肝癌细胞IGF-ⅠR在mRNA和蛋白表达水平上同步减少;干预组细胞较阴性对照组均明显抑制,2组间肝癌细胞Bel-7404 72h时抑制率比较差异有统计学意义[（61.5±1.7）%vs（11.2±0.9）%,t=5.493,P〈0.05],2组间肝癌细胞PLC/PRF/5 72 h时抑制率比较差异有统计学意义[（63.9±3.9）%vs（9.5±1.1）%,t=19.244,P〈0.001]。干预组肝癌细胞Bel-7404凋亡率显著高于空白对照组（35.96%vs 12.16%,P〈0.001）和阴性对照组（35.96%vs 9.43%,P〈0.001）,干预组肝癌细胞PLC/PRF/5凋亡率显著高于空白对照组（44.84%vs 6.62%,P〈0.001）和阴性对照组（44.84%vs 4.02%,P〈0.001）;干预组肝癌细胞Bel-7404和PLC/PRF/5 G0/G1期百分率均明显高于阴性对照组[（59.0±1.3）%vs（48.4±0.8）%,t=12.032,P〈0.001;（65.4±0.5）%vs（53.5±0.7）%,t=22.789,P〈0.001）];干预组肝癌细胞Bel-7404和PLC/PRF/5周期蛋白cyclin D1表达均明显低于阴性对照组[（59.6±4.7）%vs（90.0±3.4）%,t=7.389,P〈0.05;（39.9±0.5）%vs（90.2±14.6）%,t=4.876,P〈0.05）];肝癌细胞Bel-7404和PLC/PRF/5干预组与阴性对照组在索拉非尼浓度分别为0、2.5、5、10、20 nmol/ |
|---|---|
| AbstractList | R735.7; 目的 探讨干预胰岛素样生长因子Ⅰ型受体(IGF-ⅠR)基因转录,对抑制肝癌细胞增殖药物的协同作用.方法 以pGPU6/GFP/Neo-IGF-Ⅰ R-shRNA高效质粒,转染IGF-Ⅰ R高表达的HBV阳性肝癌细胞PLC/PRF/5和HBV阴性Bel-7404,设空白对照组、阴性对照组和IGF-Ⅰ R-shRNA干预组;以荧光定量逆转录PCR和Western Blot分析RNA和蛋白表达;以细胞计数试剂盒分析细胞增殖;以流式细胞术、Annexin-V-PE/7-ADD分析细胞周期与凋亡.计量资料组间比较采用t检验,计数资料组间比较采用Fisher确切概率法.结果 IGF-Ⅰ R shRNA转染肝癌细胞PLC/PRF/5效率为71％、肝癌细胞Bel-7404为90％,2种肝癌细胞IGF-Ⅰ R在mRNA和蛋白表达水平上同步减少;干预组细胞较阴性对照组均明显抑制,2组间肝癌细胞Bel-7404 72h时抑制率比较差异有统计学意义[(61.5±1.7)％vs(11.2±0.9)％,t=5.493,P＜0.05],2组间肝癌细胞PLC/PRF/5 72 h时抑制率比较差异有统计学意义[(63.9±3.9)％vs(9.5±1.1)％,t=19.244,P＜0.001].干预组肝癌细胞Bel-7404凋亡率显著高于空白对照组(35.96％ vs 12.16％,P＜O.001)和阴性对照组(35.96％vs 9.43％,P＜0.001),干预组肝癌细胞PLC/PRF/5凋亡率显著高于空白对照组(44.84％ vs 6.62％,P＜O.001)和阴性对照组(44.84％ vs 4.02％,P＜0.001);干预组肝癌细胞Bel-7404和PLC/PRF/5 Go/G1期百分率均明显高于阴性对照组[(59.0±1.3)％vs (48.4±0.8)％,t=12.032,P＜0.001;(65.4±0.5)％vs (53.5±0.7)％,t=22.789,P＜ 0.001)];干预组肝癌细胞Bel-7404和PLC/PRF/5周期蛋白cyclinD1表达均明显低于阴性对照组[(59.6±4.7)％vs(90.0±3.4)％,t=7.389,P＜0.05;(39.9±0.5)％ vs (90.2±14.6)％,t=4.876,P＜0.05)];肝癌细胞Bel-7404和PLC/PRF/5干预组与阴性对照组在索拉非尼浓度分 目的探讨干预胰岛素样生长因子Ⅰ型受体（IGF-ⅠR）基因转录,对抑制肝癌细胞增殖药物的协同作用。方法以p GPU6/GFP/Neo-IGF-ⅠR-shRNA高效质粒,转染IGF-ⅠR高表达的HBV阳性肝癌细胞PLC/PRF/5和HBV阴性Bel-7404,设空白对照组、阴性对照组和IGF-ⅠR-shRNA干预组;以荧光定量逆转录PCR和Western Blot分析RNA和蛋白表达;以细胞计数试剂盒分析细胞增殖;以流式细胞术、Annexin-V-PE/7-ADD分析细胞周期与凋亡。计量资料组间比较采用t检验,计数资料组间比较采用Fisher确切概率法。结果 IGF-ⅠR shRNA转染肝癌细胞PLC/PRF/5效率为71%、肝癌细胞Bel-7404为90%,2种肝癌细胞IGF-ⅠR在mRNA和蛋白表达水平上同步减少;干预组细胞较阴性对照组均明显抑制,2组间肝癌细胞Bel-7404 72h时抑制率比较差异有统计学意义[（61.5±1.7）%vs（11.2±0.9）%,t=5.493,P〈0.05],2组间肝癌细胞PLC/PRF/5 72 h时抑制率比较差异有统计学意义[（63.9±3.9）%vs（9.5±1.1）%,t=19.244,P〈0.001]。干预组肝癌细胞Bel-7404凋亡率显著高于空白对照组（35.96%vs 12.16%,P〈0.001）和阴性对照组（35.96%vs 9.43%,P〈0.001）,干预组肝癌细胞PLC/PRF/5凋亡率显著高于空白对照组（44.84%vs 6.62%,P〈0.001）和阴性对照组（44.84%vs 4.02%,P〈0.001）;干预组肝癌细胞Bel-7404和PLC/PRF/5 G0/G1期百分率均明显高于阴性对照组[（59.0±1.3）%vs（48.4±0.8）%,t=12.032,P〈0.001;（65.4±0.5）%vs（53.5±0.7）%,t=22.789,P〈0.001）];干预组肝癌细胞Bel-7404和PLC/PRF/5周期蛋白cyclin D1表达均明显低于阴性对照组[（59.6±4.7）%vs（90.0±3.4）%,t=7.389,P〈0.05;（39.9±0.5）%vs（90.2±14.6）%,t=4.876,P〈0.05）];肝癌细胞Bel-7404和PLC/PRF/5干预组与阴性对照组在索拉非尼浓度分别为0、2.5、5、10、20 nmol/ |
| Author | 蔡胤 姚敏 王理 董志珍 顾娟娟 严晓娣 邱历伟 姚登福 |
| AuthorAffiliation | 南通大学附属医院临床医学研究中心,江苏南通226001 |
| AuthorAffiliation_xml | – name: 南通大学附属医院临床医学研究中心,江苏南通,226001 |
| Author_xml | – sequence: 1 fullname: 蔡胤 姚敏 王理 董志珍 顾娟娟 严晓娣 邱历伟 姚登福 |
| BookMark | eNo9kF9LwlAAxe-DQWZ-ieihF9fdvbt322NI_0DoxXfZ3GYTm-WIyLfSxEzRyJSyTHyqh_4gUoRI3yW82_wWLYyeDhx-nHM4CyBgZS0dgGUeclim8mqaM23b4ngI-QhBhHII8pSDEgcRCoDgvz8PwrZtqpDwmMoCoUGQZZ-Dab_oFd7YoOMOe07vw20-TK-_WKfHnhvfZz3WvWD19mR85QxHrNryTpqsUXYqbfem6tVe3fMnp3LJyu_e6b3vuKOSV-iyftd5abm3RVars0Z1Mr5zm4-sXHK6jUUwZygZWw__aQjEN9bj0a1IbGdzO7oWiyQpRBFVhpK_UsIqErGsE0XUkSFoBtZUDDUsKhLCmGBMoS4axNAohZIsqJgIsqpCBHEIrMxijxTLUKxUIp09zFl-YSKTTGlqPv97EJT8e3x0aYYmd7NW6sD04f2cuafkjhOUyqJEBIrxD1UFkWE |
| ClassificationCodes | R735.7 |
| ContentType | Journal Article |
| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| Copyright_xml | – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| DBID | 2RA 92L CQIGP W91 ~WA 2B. 4A8 92I 93N PSX TCJ |
| DOI | 10.3969/j.issn.1001-5256.2016.08.022 |
| DatabaseName | 维普期刊资源整合服务平台 中文科技期刊数据库-CALIS站点 中文科技期刊数据库-7.0平台 中文科技期刊数据库-医药卫生 中文科技期刊数据库- 镜像站点 Wanfang Data Journals - Hong Kong WANFANG Data Centre Wanfang Data Journals 万方数据期刊 - 香港版 China Online Journals (COJ) China Online Journals (COJ) |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine |
| DocumentTitleAlternate | Synergistic effect of interventing insulin-like growth factor-Ⅰ receptor activation combined with anti-cancer drugs in inhibiting the proliferation of hepatocellular carcinoma cells |
| DocumentTitle_FL | Synergistic effect of interventing insulin-like growth factor-I receptor activation combined with anti-cancer drugs in inhibiting the proliferation of hepatocellular carcinoma cells |
| EndPage | 1548 |
| ExternalDocumentID | lcgdbzz201608022 669785463 |
| GrantInformation_xml | – fundername: 国家国际科技合作项目; 南通市科技项目 funderid: (2013DFA32150); (HS2014078) |
| GroupedDBID | -05 2B. 2C~ 2RA 5XA 5XF 92F 92I 92L ABDBF ACGFS ALMA_UNASSIGNED_HOLDINGS CCEZO CIEJG CQIGP CW9 EAD EAP EOJEC ESX GROUPED_DOAJ IPNFZ OBODZ OK1 RIG RNS TCJ TGQ U1G U5O W91 ~WA 4A8 93N ABJNI ACUHS PSX |
| ID | FETCH-LOGICAL-c602-b908b0583b2739e5a7e2f4df3db30d37a823353360e7f5fd660894b3549bb0203 |
| ISSN | 1001-5256 |
| IngestDate | Thu May 29 03:59:50 EDT 2025 Wed Feb 14 10:23:55 EST 2024 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 8 |
| Keywords | 索拉非尼 sorafenib 受体,IGFI型 奥沙利铂 receptor,IGF type 1 carcinoma,hepatocellular 癌,肝细胞 oxaliplation |
| Language | Chinese |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c602-b908b0583b2739e5a7e2f4df3db30d37a823353360e7f5fd660894b3549bb0203 |
| Notes | carcinoma; hepatocellular; receptor; IGF type 1; sorafenib; oxaliplation CAI Yin, YAO Min, WANG Li, et al. （ Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, China） Objective To investigate the intervention of gene transcription of insulin- like growth factor- Ⅰ receptor（ IGF- ⅠR） and its synergistic effect with anti- cancer drugs in inhibiting the proliferation of hepatocellular carcinoma（ HCC） cells. Methods The HBV-positive HCC PLC / PRF /5 and HBV- negative Bel- 7404 cells were transfected with the efficient plasmid p GPU6 / GFP / Neo- IGF- ⅠR-shRNA. Fluorescent quantitative RT- PCR and Western blot were used to measure mRNA and protein expression,the Cell Counting Kit-8 was used to analyze cell proliferation,and flow cytometry and Annexin- V- PE /7- ADD were used to analyze cell cycle and apoptosis.The t- test was used for comparison of continuous data between groups,the Fisher＇s exact test was used for comparison of categorical data between groups. |
| PageCount | 6 |
| ParticipantIDs | wanfang_journals_lcgdbzz201608022 chongqing_primary_669785463 |
| PublicationCentury | 2000 |
| PublicationDate | 2016 |
| PublicationDateYYYYMMDD | 2016-01-01 |
| PublicationDate_xml | – year: 2016 text: 2016 |
| PublicationDecade | 2010 |
| PublicationTitle | 临床肝胆病杂志 |
| PublicationTitleAlternate | Chinese Journal of Clinical Hepatology |
| PublicationTitle_FL | Journal of Clinical Hepatology |
| PublicationYear | 2016 |
| Publisher | 南通大学附属医院临床医学研究中心,江苏南通,226001 |
| Publisher_xml | – name: 南通大学附属医院临床医学研究中心,江苏南通,226001 |
| SSID | ssib051369456 ssj0041983 ssib001103858 ssib053243598 ssib002264326 ssib038074677 |
| Score | 2.0449865 |
| Snippet | 目的探讨干预胰岛素样生长因子Ⅰ型受体（IGF-ⅠR）基因转录,对抑制肝癌细胞增殖药物的协同作用。方法以p GPU6/GFP/Neo-IGF-ⅠR-shRNA高效质粒,转染IGF-ⅠR高表达的HBV阳性肝癌... R735.7; 目的 探讨干预胰岛素样生长因子Ⅰ型受体(IGF-ⅠR)基因转录,对抑制肝癌细胞增殖药物的协同作用.方法 以pGPU6/GFP/Neo-IGF-Ⅰ R-shRNA高效质粒,转染IGF-Ⅰ R高表达的HBV... |
| SourceID | wanfang chongqing |
| SourceType | Aggregation Database Publisher |
| StartPage | 1543 |
| SubjectTerms | 受体,IGFⅠ型 奥沙利铂 癌,肝细胞 索拉非尼 |
| Title | 干预胰岛素样生长因子Ⅰ型受体活化联合抗癌药物抑制肝癌细胞增殖的协同作用分析 |
| URI | http://lib.cqvip.com/qk/90100X/201608/669785463.html https://d.wanfangdata.com.cn/periodical/lcgdbzz201608022 |
| Volume | 32 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| journalDatabaseRights | – providerCode: PRVEBS databaseName: EBSCOhost Academic Search Ultimate issn: 1001-5256 databaseCode: ABDBF dateStart: 20130401 customDbUrl: https://search.ebscohost.com/login.aspx?authtype=ip,shib&custid=s3936755&profile=ehost&defaultdb=asn isFulltext: true dateEnd: 99991231 titleUrlDefault: https://search.ebscohost.com/direct.asp?db=asn omitProxy: true ssIdentifier: ssj0041983 providerName: EBSCOhost – providerCode: PRVHPJ databaseName: ROAD: Directory of Open Access Scholarly Resources issn: 1001-5256 databaseCode: M~E dateStart: 19850101 customDbUrl: isFulltext: true dateEnd: 99991231 titleUrlDefault: https://road.issn.org omitProxy: true ssIdentifier: ssib053243598 providerName: ISSN International Centre |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnR1NTxNBdIMlIV6MnxFRgwlzLO7H7OzMcbfdDTHBU024kW63CwdT_IBLbwoSRAg1IkTR2nDSgx8hRGMI8b8Yti3_wvdmt-1CiEEvm2Xmfc17Q997m3lvFGVEtULmW0Y5S0WRZakpzCwPaBH-LFEaBODjNKwdHr_Lxu7ROxPmRF-mkDq1NDfrj5aqJ9aV_I9VYQzsilWy_2DZLlEYgHewLzzBwvA8lY2JaxJHEEcnriC2TjglLifcII4qp3QiHOJaxKHEhhGGT8fCEUGJ8BBLAJiHwABpSyw7T4Qqz0CYyYhwCXfwhXtEADolDsAYSBAoO_FUjggmuWtIHLFUwjnCcFtiAVOBYAiTJ7aHIxzEFh0YTdLhxInpgPD5FJaFjDhLFggioai6fIF1uZI7ANtSCSay4F5HjFxH5lyydpsnvJAgQyLxbaKdMF3Cc1wdqtFG8XoiSQF4zM5CLSGFPM66Upmi69oRFrlpCZJNezMgvRQXkVGzvRmgGSvcksKzI9Q0YhtdPhLWg7Wmv93ERaXy_yxRBAIKeaTFlnwpso4tbTO5CUSiNUdLNAsqQ7vKKdDyCerownTpWLhd0PYWGhWtKLFgNpYXFKDn5KbR5ObjuMjYSMeExFumMUROeUw8k2fqcXf4jkvtfbKe6xzJjv0jBOxGKtbCfPkkP24IJqQfRxajXRZ4EpPJjrtxLfuxTun3S1OBX60ikKwgP6P06_iVLqP0207e8XppgqYe6eGExeSpNAbvYKCpNo-mZjBBez2nTEhCsPFlJ-KjmuBxIU8i54Aykizi9t-WgO1cpmcqUw8hSJU1g5WwWJlKhbeF88q5JC8dtuMfmQtKX3X6ojIwnpy8uaTMRD93DrcX2vPfop2t1m6j2fjRWv9w-PpXtNWIPtd-P2tE9RfR2ubB_qvm7l60stF-sh7VlprLm603K-3Vr63nn5rLL6Ol7-2n72GktbfYnq9H2_Xml43W24VodS2qrRzsv2utf4yWFpv12mWl4LmF3Fg2uawlW2IQVPlC5aAZbviQD4myWbTKekiD0Ah8Qw0Mq8h1w4DUkqllKzRDcAIqF9Q3TCp8H09DXFEylZlK-aoyzDgmJuUSxD8BDUNWpFoQUgqRCAePw9RBZairtskHcU-eScaExfFqj0HlVqLIyeSX-vHk8Y1x7RQwQ8pZfI-_tl5XMrOP5so3IP-Y9W8m2-kPAhDxCw |
| linkProvider | EBSCOhost |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E5%B9%B2%E9%A2%84%E8%83%B0%E5%B2%9B%E7%B4%A0%E6%A0%B7%E7%94%9F%E9%95%BF%E5%9B%A0%E5%AD%90%E2%85%A0%E5%9E%8B%E5%8F%97%E4%BD%93%E6%B4%BB%E5%8C%96%E8%81%94%E5%90%88%E6%8A%97%E7%99%8C%E8%8D%AF%E7%89%A9%E6%8A%91%E5%88%B6%E8%82%9D%E7%99%8C%E7%BB%86%E8%83%9E%E5%A2%9E%E6%AE%96%E7%9A%84%E5%8D%8F%E5%90%8C%E4%BD%9C%E7%94%A8%E5%88%86%E6%9E%90&rft.jtitle=%E4%B8%B4%E5%BA%8A%E8%82%9D%E8%83%86%E7%97%85%E6%9D%82%E5%BF%97&rft.au=%E8%94%A1%E8%83%A4&rft.au=%E5%A7%9A%E6%95%8F&rft.au=%E7%8E%8B%E7%90%86&rft.au=%E8%91%A3%E5%BF%97%E7%8F%8D&rft.date=2016&rft.pub=%E5%8D%97%E9%80%9A%E5%A4%A7%E5%AD%A6%E9%99%84%E5%B1%9E%E5%8C%BB%E9%99%A2%E4%B8%B4%E5%BA%8A%E5%8C%BB%E5%AD%A6%E7%A0%94%E7%A9%B6%E4%B8%AD%E5%BF%83%2C%E6%B1%9F%E8%8B%8F%E5%8D%97%E9%80%9A%2C226001&rft.issn=1001-5256&rft.volume=32&rft.issue=8&rft.spage=1543&rft.epage=1548&rft_id=info:doi/10.3969%2Fj.issn.1001-5256.2016.08.022&rft.externalDocID=lcgdbzz201608022 |
| thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fimage.cqvip.com%2Fvip1000%2Fqk%2F90100X%2F90100X.jpg http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Flcgdbzz%2Flcgdbzz.jpg |