一株嗜酸氧化亚铁硫杆菌的鉴定及浸矿性能

从西藏甲玛某多金属矿的酸性矿坑水中分离获得一株浸矿细菌(命名为XZ).该菌为革兰氏阴性菌,短杆状,菌体长约1—1.5μm,直径约0.5μm,两端钝圆,能够氧化Fe2+和单质硫,最适生长pH为2.0,最适生长温度为30℃,经过对其生长特性进行研究及16SrRNA基因测序鉴定其为一株嗜酸氧化亚铁硫杆菌.利用XZ菌对西藏某低品位铜矿角岩矿进行了摇瓶浸出试验研究,结果表明,在接种量体积分数为10%,矿浆质量分数为10%,矿石粒度为-45μm占100%,培养温度30℃,振荡强度160r/min的浸出条件下,经过15d,Cu2+的浸出率达72.15%....

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Bibliographic Details
Published in东北大学学报(自然科学版) Vol. 37; no. 1; pp. 89 - 93
Main Author 卢涛 魏德洲 张瑞洋 卓建英
Format Journal Article
LanguageChinese
Published 东北大学资源与土木工程学院,辽宁沈阳,110819%辽宁省冶金地质勘查局四○三队,辽宁鞍山,114018 2016
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ISSN1005-3026
DOI10.3969/j.issn.1005-3026.2016.01.019

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Summary:从西藏甲玛某多金属矿的酸性矿坑水中分离获得一株浸矿细菌(命名为XZ).该菌为革兰氏阴性菌,短杆状,菌体长约1—1.5μm,直径约0.5μm,两端钝圆,能够氧化Fe2+和单质硫,最适生长pH为2.0,最适生长温度为30℃,经过对其生长特性进行研究及16SrRNA基因测序鉴定其为一株嗜酸氧化亚铁硫杆菌.利用XZ菌对西藏某低品位铜矿角岩矿进行了摇瓶浸出试验研究,结果表明,在接种量体积分数为10%,矿浆质量分数为10%,矿石粒度为-45μm占100%,培养温度30℃,振荡强度160r/min的浸出条件下,经过15d,Cu2+的浸出率达72.15%.
Bibliography:LU Tao, WEI De-zhou , ZHANG Rui-yang , ZHUO Jian-ying( 1. School of Resources & Civil Engineering, Northeastern University, Shenyang 110819, China; 2. Team 403 in Liaoning Provincial Bureau of Metallurgical & Geological Prospecting, Anshan 114018, China. Corresponding author: LU Tao, E-mail: It_heaven008@ 163. com)
21-1344/T
Acidithiobacillus ferrooxidans ; strain identification; 16S rRNA; bioleaching ; poorcopper ore
A bioleaching bacterium was isolated from an acidic mine drainage of Jiama polymetallic mine in Tibet and was named as XZ. The strain XZ, with round ends, is gram- negative and short-rod shaped in 1 - 1.5 μm × 0. 5 μm. Ferrous iron and elemental sulfur could be oxidized by strain XZ. The optimum growth pH is 2.0 and the optimum growth temperature is 30℃, It was identified as Acidithiobacillus ferrooxidans by means of growth characteristic research and 16 S rRNA sequencing. The strain XZ was used to leach a poor copper hornstone ore took from Tibet. Shaking flask leaching tests were carded out with
ISSN:1005-3026
DOI:10.3969/j.issn.1005-3026.2016.01.019