芦苇秸秆厌氧联产氢气甲烷过程中细菌群落演替规律

基于PCR-DGGE技术研究芦苇秸秆氢气-甲烷厌氧联产过程中,细菌微生物群落结构特征和演替规律。结果表明,厌氧联产过程中细菌群落结构分布存在明显的阶段性差异。产氢阶段初期,细菌群落相似性较小,随着厌氧联产的进行,细菌种类逐渐增多并在产氢高峰期保持稳定,群落相似性较高,戴斯系数（Cs）为83.6%（第12、24小时,泳道H3和H4）。产甲烷高峰期Cs值达到87.4%（第210、258小时,泳道M6,M7）,群落结构稳定,产甲烷末期Cs值降低至51.5%（第210、432小时,泳道M6,M9）。序列分析表明,Enterobacter aerogenes产气肠杆菌是具有高效产氢潜力的兼性厌氧细菌,S...

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Published in农业工程学报 Vol. 32; no. 4; pp. 199 - 204
Main Author 贾璇 任连海 李鸣晓 席北斗 祝超伟 赵由才
Format Journal Article
LanguageChinese
Published 北京工商大学环境科学与工程系,北京,100048%中国环境科学研究院环境基准与风险评估国家重点实验室,北京,100012%同济大学污染控制与资源化研究国家重点实验室,上海,200092 2016
Subjects
氢气
甲烷
秸秆
细菌
群落演替
芦苇
秸秆
芦苇
bacteria
群落演替
reed
methane
细菌
甲烷
hydrogen
straw
氢气
community succession
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ISSN1002-6819
DOI10.11975/j.issn.1002-6819.2016.04.028

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Summary:基于PCR-DGGE技术研究芦苇秸秆氢气-甲烷厌氧联产过程中,细菌微生物群落结构特征和演替规律。结果表明,厌氧联产过程中细菌群落结构分布存在明显的阶段性差异。产氢阶段初期,细菌群落相似性较小,随着厌氧联产的进行,细菌种类逐渐增多并在产氢高峰期保持稳定,群落相似性较高,戴斯系数（Cs）为83.6%（第12、24小时,泳道H3和H4）。产甲烷高峰期Cs值达到87.4%（第210、258小时,泳道M6,M7）,群落结构稳定,产甲烷末期Cs值降低至51.5%（第210、432小时,泳道M6,M9）。序列分析表明,Enterobacter aerogenes产气肠杆菌是具有高效产氢潜力的兼性厌氧细菌,Sedimentibacter产氢产乙酸菌是产氢阶段的优势微生物。Clostridium thermocellum嗜热纤维素菌是厌氧联产过程的优势微生物,具有降解纤维素功能,对芦苇秸秆的能源化利用起到重要作用。
Bibliography:11-2047/S
The two-stage coproduction of hydrogen and methane using cellulosic biomass, such as reed straw, is a promising technology for achieving energy saving and emission reduction and developing a circular economy. The enhancement of hydrogen and methane coproduction from reed straw under enzyme pretreatment was evaluated during anaerobic fermentation. The effects of cellulase pretreatment on biogas production performance and intermediate metabolites’ characteristics were investigated in this study. In addition, the combination of polymerase chain reaction （PCR） amplification of 16S rRNA genes with denaturing gradient gel electrophoresis （DGGE） analysis was used to study the composition and succession of bacterial community in fermentative biohydrogen with methanogenesis integration system. The results showed that the maximum accumulative biogas production and hydrogen proportion were 42.5 mL/g and 52.1% respectively in hydrogenogenic stage. And the maximum accumulative biogas production of 137.5 mL/g was
ISSN:1002-6819
DOI:10.11975/j.issn.1002-6819.2016.04.028