南海区卵形鲳鲹遗传多样性的研究

为研究南海区4个不同地理位置卵形鲳鲹种群（QZ、ZJ、BH、HN）的遗传多样性.利用35对可扩增出特异性条带的引物对卵形鲳鲹4个不同地理种群进行遗传多样性分析,筛选出10对扩增效果比较好的引物,其中7对引物具有多态性.7个微卫星多态位点中,一共扩增出21个等位基因;多态信息含量为0.2896～ 0.6914,且处于中度到高度多态,表明这些引物可作为卵形鲳鲹遗传多样性分析的有效微卫星引物;4个群体的平均期望杂合度在0.4811～0.6135,北海群体的平均期望杂合度小于0.5,表明北海卵形鲳鲹群体的遗传多样性较低.用Popgene32软件计算出4个种群卵形鲳鲹的标准遗传距离和相似性系数,并构建聚...

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Bibliographic Details
Published in西南农业学报 Vol. 27; no. 4; pp. 1786 - 1790
Main Author 赵永贞 陈秀荔 李咏梅 彭敏 杨春玲 韦嫔媛 彭金霞 陈晓汉
Format Journal Article
LanguageChinese
Published 广西水产遗传育种与健康养殖重点实验室,广西壮族自治区水产科学研究院,广西南宁530021 2014
Subjects
卵形鲳鲹
微卫星分子标记
遗传多样性
卵形鲳鲹
Genetic diversity
Trachinotus ovatus
遗传多样性
Microsatelite marker
微卫星分子标记
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ISSN1001-4829

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Summary:为研究南海区4个不同地理位置卵形鲳鲹种群（QZ、ZJ、BH、HN）的遗传多样性.利用35对可扩增出特异性条带的引物对卵形鲳鲹4个不同地理种群进行遗传多样性分析,筛选出10对扩增效果比较好的引物,其中7对引物具有多态性.7个微卫星多态位点中,一共扩增出21个等位基因;多态信息含量为0.2896～ 0.6914,且处于中度到高度多态,表明这些引物可作为卵形鲳鲹遗传多样性分析的有效微卫星引物;4个群体的平均期望杂合度在0.4811～0.6135,北海群体的平均期望杂合度小于0.5,表明北海卵形鲳鲹群体的遗传多样性较低.用Popgene32软件计算出4个种群卵形鲳鲹的标准遗传距离和相似性系数,并构建聚类树,发现湛江卵形鲳鲹群体和海南群体遗传相似性系数最大,遗传关系最近.
Bibliography:51-1213/S
ZHAO Yong-zhen, CHEN Xiu-li, LI Yong-mei , PENG Min, YANG Chun-ling,WEI Pin-yuan, PENG Jin-xia,CHEN Xiao-han （ Guangxi Academy of Fishery Sciences, Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Nanning 530021, China）
The genetic diversity of 4 different Trachinotus ovatus groups in Nanhai district （ QZ, ZJ, BH,HN） was analyzed by using the selected micro satellite sequences. 35 pairs of primers were amplified successfully and every primer could produce a single, bright band, which were used to analyze the genetic diversity of 4 different Trach/notus ovatus groups in Nanhai district. The result that the amplification effect of 10 pairs of polymorphic primers was batter, in which 7 pairs of primers expressed polymorphic, we amplified 21 alleles in 4 groups. In 4 dif- ferent Trachinotus ovatus groups, the number of the effective allele was 1 -3. 8413, on the average of 2.40; the information content （PIC） was 0. 2896 -0. 6914, the result indicated that these molecular
ISSN:1001-4829