一种快速高效提取甘蔗及其近缘属基因组DNA的方法
利用珠磨器和不锈钢珠，以甘蔗及其近缘属割手密、斑茅和河八王为材料提取基因组DNA，并对得到的DNA进行电泳检测、含量测定和SSR分析。结果表明，该方法能高效快速提取基因组DNA，所提取DNA中蛋白质、多糖类物质等去除较彻底，DNA纯度较高，完整性好；SSR分析条带清晰，多态性较丰富，能扩增出特异性谱带。该方法无需液氮研磨，简单、快速、高效，经济成本低、PCR扩增效果好，适用于大规模的样品检测，可用于甘蔗育种遗传多样性分析、种质鉴定及指纹图谱构建等。...
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| Published in | Xinan nongye xuebao Vol. 23; no. 2; pp. 515 - 518 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
广西甘蔗研究所,广西,南宁,530007%广西大学农学院,广西,南宁,530004
2010
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1001-4829 |
| DOI | 10.3969/j.issn.1001-4829.2010.02.048 |
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| Abstract | 利用珠磨器和不锈钢珠，以甘蔗及其近缘属割手密、斑茅和河八王为材料提取基因组DNA，并对得到的DNA进行电泳检测、含量测定和SSR分析。结果表明，该方法能高效快速提取基因组DNA，所提取DNA中蛋白质、多糖类物质等去除较彻底，DNA纯度较高，完整性好；SSR分析条带清晰，多态性较丰富，能扩增出特异性谱带。该方法无需液氮研磨，简单、快速、高效，经济成本低、PCR扩增效果好，适用于大规模的样品检测，可用于甘蔗育种遗传多样性分析、种质鉴定及指纹图谱构建等。 |
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| AbstractList | 利用珠磨器和不锈钢珠，以甘蔗及其近缘属割手密、斑茅和河八王为材料提取基因组DNA，并对得到的DNA进行电泳检测、含量测定和SSR分析。结果表明，该方法能高效快速提取基因组DNA，所提取DNA中蛋白质、多糖类物质等去除较彻底，DNA纯度较高，完整性好；SSR分析条带清晰，多态性较丰富，能扩增出特异性谱带。该方法无需液氮研磨，简单、快速、高效，经济成本低、PCR扩增效果好，适用于大规模的样品检测，可用于甘蔗育种遗传多样性分析、种质鉴定及指纹图谱构建等。 S566.1; 利用珠磨器和不锈钢珠,以甘蔗及其近缘属割手密、斑茅和河八王为材料提取基因组DNA,并对得到的DNA进行电泳检测、含量测定和SSR分析.结果表明,该方法能高效快速提取基因组DNA,所提取DNA中蛋白质、多糖类物质等去除较彻底,DNA纯度较高,完整性好;SSR分析条带清晰,多态性较丰富,能扩增出特异性谱带.该方法无需液氮研磨,简单、快速、高效,经济成本低、PCR扩增效果好,适用于大规模的样品检测,可用于甘蔗育种遗传多样性分析、种质鉴定及指纹图谱构建等. |
| Author | 刘昔辉 宋焕忠 张革民 张荣华 方位宽 区惠平 陆建勋 梁强 唐红琴 |
| AuthorAffiliation | 广西甘蔗研究所,广西南宁530007 广西大学农学院,广西南宁530004 |
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| Author_FL | OU Hui-ping LIANG Qiang TANG Hong-qin ZHANG Rong-hua LU Jian-xun ZHANG Ge-min FANG Wei-kuan LIU Xi-hui SONG Huan-zhong |
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| ClassificationCodes | S566.1 |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| PublicationYear | 2010 |
| Publisher | 广西甘蔗研究所,广西,南宁,530007%广西大学农学院,广西,南宁,530004 |
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| Title | 一种快速高效提取甘蔗及其近缘属基因组DNA的方法 |
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