红壤区油茶根际解磷细菌的筛选、鉴定及其解磷能力

研究油茶根际解无机磷细菌的解磷能力对提高红壤丘陵区土壤磷素利用效率具有重要的指导意义.本研究从油茶根际分离筛选出97株具有解无机磷能力的菌株,采用NBRI-BPB培养基进行复筛获得5株解磷能力较强的解无机磷细菌,并测定其在NBRIP培养基中有效磷含量,采用形态特征、生理生化、Biolog系统和16SrDNA序列分析鉴定细菌种类,确定WB38为耳假单胞菌（Pseudomonas auricularis）,WB39（WB75）为杓兰果胶杆菌（Pectobacterium cpripedii）,WB53（WB68）为路德维希肠杆菌（Enterobacter ludwigii）.研究了解磷菌株WB38...

Full description

Saved in:
Bibliographic Details
Published in林业科学研究 Vol. 28; no. 3; pp. 409 - 416
Main Author 王舒 张林平 张扬 郝菲菲 肖金香 胡冬南
Format Journal Article
LanguageChinese
Published 江西农业大学园林与艺术学院,江西南昌,330045 2015
Subjects
Biolog系统
油茶
解磷细菌
解磷能力
油茶
Biolog system
Camellia oleifera
16S rDNA
phosphate-solubilizing bacteria
解磷能力
phosphate-solubilizing capability
解磷细菌
Biolog系统
16SrDNA
Online AccessGet full text
ISSN1001-1498

Cover

More Information
Summary:研究油茶根际解无机磷细菌的解磷能力对提高红壤丘陵区土壤磷素利用效率具有重要的指导意义.本研究从油茶根际分离筛选出97株具有解无机磷能力的菌株,采用NBRI-BPB培养基进行复筛获得5株解磷能力较强的解无机磷细菌,并测定其在NBRIP培养基中有效磷含量,采用形态特征、生理生化、Biolog系统和16SrDNA序列分析鉴定细菌种类,确定WB38为耳假单胞菌（Pseudomonas auricularis）,WB39（WB75）为杓兰果胶杆菌（Pectobacterium cpripedii）,WB53（WB68）为路德维希肠杆菌（Enterobacter ludwigii）.研究了解磷菌株WB38在不同条件对解磷能力的影响,结果表明：不同因子对该菌株解磷能力均有显著影响,其中温度对其解磷能力影响最大;该菌株培养的最适碳源和氮源分别为葡萄糖和NH4NO3;解磷细菌WB38解磷的最佳培养条件为A2B1C1D1,即温度28℃,初始pH值6.5,接种量1％,溶氧量25 mL.
Bibliography:WANG Shu, ZHANG Lin-ping, ZHANG Yang, HAO Fei-fei, XIAO Jin-xiang, ttU Dong-nan （College of Landscape and Art,Jiangxi Agricultural University, Nanchang 330045, Jiangxi, China）
11-1221/S
Camellia oleifera ; phosphate-solubilizing bacteria ; phosphate-solubilizing capability ; 16S rDNA ; Biolog system
The phosphate solubilizing capability of phosphate solubilizing bacteria （PSB） in rhizosphere of Camellia oleifera Abel apparently affects the Phosphorus use efficiency in red soil. 97 phosphate-solubilizing bacterial strains were isolated from the Camellia oleifera rhizosphere soil of Hu＇ nan Province, the high effective PSB were further screened using NBRI-BPB medium, and the molybdenum-anti-spectrophotometric method was applied to determine the phosphate-dissolving ability of the high effective PSB after four days＇ fermentation in NBRIP medium. The bac- teria were identified by their morphological, physiological and biochemical characteristics with Biolog system and 16S rDNA gene sequence analysis. The high effect
ISSN:1001-1498