刺玫叶总皂苷富集工艺条件优化

以吸附率和解吸率为考查指标,采用静态吸附-解吸、动态吸附-解吸试验,确定了D-101型大孔树脂富集刺玫叶总皂苷的工艺条件。结果发现,最佳富集条件为：上柱液浓度为原提取液稀释5倍、吸附速率为2 BV/h、上柱液p H值为7~9,解吸液为95%乙醇、洗脱速率为2 BV/h、洗脱液用量为7 BV。在此条件下,其平均吸附率为81.33%,平均解吸率为86.31%,纯化后刺玫叶干浸膏中总皂苷含量由原来的16.32%提高到48.13%,树脂富集倍数约为3倍。结果表明,D-101型大孔树脂对刺玫叶总皂苷的富集具有一定的效果。...

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Published in广东农业科学 Vol. 42; no. 8; pp. 77 - 82
Main Author 王晓林 杜晓宇 钟方丽 徐欣
Format Journal Article
LanguageChinese
Published 吉林化工学院化学与制药工程学院,吉林吉林,132022 2015
Subjects
刺玫叶
大孔树脂
富集
总皂苷
总皂苷
富集
大孔树脂
刺玫叶
Online AccessGet full text
ISSN1004-874X

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Summary:以吸附率和解吸率为考查指标,采用静态吸附-解吸、动态吸附-解吸试验,确定了D-101型大孔树脂富集刺玫叶总皂苷的工艺条件。结果发现,最佳富集条件为：上柱液浓度为原提取液稀释5倍、吸附速率为2 BV/h、上柱液p H值为7~9,解吸液为95%乙醇、洗脱速率为2 BV/h、洗脱液用量为7 BV。在此条件下,其平均吸附率为81.33%,平均解吸率为86.31%,纯化后刺玫叶干浸膏中总皂苷含量由原来的16.32%提高到48.13%,树脂富集倍数约为3倍。结果表明,D-101型大孔树脂对刺玫叶总皂苷的富集具有一定的效果。
Bibliography:44-1267/S
To investigate the enrichment technology of total saponins （TS） in leaves of Rosa davurica Pall. with macroporous resin, by both static and dynamic absorption-desorption experiments, the optimum purification conditions with D-101 macroporous resin were determined using absorption and desorption ratio of TS as evaluating indicators. The results showed that the optinal enrichment canditions were as follows： the TS content of the sample, adsorption rate, pH value of sample, content of ethanol eluant, desorption rate and volume of eluant were one-fifth content of the raw extracts solution, 2 BV/h, 7 to 9, 70%, 2 BV/h, and 7 BV respectively. The average absorption and desorption rates were 81.33% and 86.31% under the optimum conditions. After purification, the TS content in extracts was from 16.32% to 48.13%, which offered 3 times of enrichment with resins. The results indicated that purification of TS in leaves of R. davurica Pall. with D-101 macroporous resin was effective.
WANG Xiao-lin, DU Xiao-yu, ZHO
ISSN:1004-874X