磷脂酰肌醇蛋白多糖-3对肝癌细胞增殖和凋亡的影响
目的探讨shRNA干预磷脂酰肌醇蛋白多糖（GPC）-3基因转录对肝癌细胞增殖和凋亡的影响。方法将GPC-3-shRNA插入pGPU6／GFP／Neo质粒，转染人肝癌tlepG2细胞，以Western Blot和荧光定量PCR分别分析GPC-3蛋白和mRNA表达；以nTr法分析HepG2细胞增殖；以流式细胞术、Annexin—V—PE／7-AAD及细胞凋亡-DNA ladder等分析细胞周期及细胞凋亡率。结果shRNA1转染HepG2细胞，GPC-3mRNA沉默效率为89．3％，与GPC-3蛋白下调一致；以shRNA转染HepG2细胞，增殖抑制率为71．1％；细胞周期阻滞在G1期，细胞凋亡率达6...
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| Published in | 临床肝胆病杂志 Vol. 29; no. 11; pp. 863 - 866 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
南通大学附属医院,临床医学研究中心,江苏,南通,226001
2013
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1001-5256 |
| DOI | 10.3969/j.issn.1001-5256.2013.11.015 |
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| Abstract | 目的探讨shRNA干预磷脂酰肌醇蛋白多糖（GPC）-3基因转录对肝癌细胞增殖和凋亡的影响。方法将GPC-3-shRNA插入pGPU6／GFP／Neo质粒，转染人肝癌tlepG2细胞，以Western Blot和荧光定量PCR分别分析GPC-3蛋白和mRNA表达；以nTr法分析HepG2细胞增殖；以流式细胞术、Annexin—V—PE／7-AAD及细胞凋亡-DNA ladder等分析细胞周期及细胞凋亡率。结果shRNA1转染HepG2细胞，GPC-3mRNA沉默效率为89．3％，与GPC-3蛋白下调一致；以shRNA转染HepG2细胞，增殖抑制率为71．1％；细胞周期阻滞在G1期，细胞凋亡率达65．6％。结论资料显示shRNA干预GPC-3基因转录，可显著抑制肝癌细胞增殖，促进肝癌细胞凋亡。 |
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| AbstractList | 目的探讨shRNA干预磷脂酰肌醇蛋白多糖（GPC）-3基因转录对肝癌细胞增殖和凋亡的影响。方法将GPC-3-shRNA插入pGPU6／GFP／Neo质粒，转染人肝癌tlepG2细胞，以Western Blot和荧光定量PCR分别分析GPC-3蛋白和mRNA表达；以nTr法分析HepG2细胞增殖；以流式细胞术、Annexin—V—PE／7-AAD及细胞凋亡-DNA ladder等分析细胞周期及细胞凋亡率。结果shRNA1转染HepG2细胞，GPC-3mRNA沉默效率为89．3％，与GPC-3蛋白下调一致；以shRNA转染HepG2细胞，增殖抑制率为71．1％；细胞周期阻滞在G1期，细胞凋亡率达65．6％。结论资料显示shRNA干预GPC-3基因转录，可显著抑制肝癌细胞增殖，促进肝癌细胞凋亡。 R735.7; 目的 探讨shRNA干预磷脂酰肌醇蛋白多糖(GPC)-3基因转录对肝癌细胞增殖和凋亡的影响.方法 将GPC-3-shRNA插入pGPU6/GFP/Neo质粒,转染人肝癌HepG2细胞,以Western Blot和荧光定量PCR分别分析GPC-3蛋白和mRNA表达;以MTT法分析HepG2细胞增殖;以流式细胞术、Annexin-V-PE/7-AAD及细胞凋亡-DNA ladder等分析细胞周期及细胞凋亡率.结果 shRNA1转染HepG2细胞,GPC-3 mRNA沉默效率为89.3%,与GPC-3蛋白下调一致;以shRNA转染HepG2细胞,增殖抑制率为71.1%;细胞周期阻滞在G1期,细胞凋亡率达65.6%.结论 资料显示shRNA干预GPC-3基因转录,可显著抑制肝癌细胞增殖,促进肝癌细胞凋亡. |
| Author | 邰伯军 姚敏 顾星 时运 蔚丹丹 陈洁 郑文杰 姚登福 陆少林 |
| AuthorAffiliation | 南通大学附属医院临床医学研究中心,江苏南通226001 |
| AuthorAffiliation_xml | – name: 南通大学附属医院,临床医学研究中心,江苏,南通,226001 |
| Author_xml | – sequence: 1 fullname: 邰伯军 姚敏 顾星 时运 蔚丹丹 陈洁 郑文杰 姚登福 陆少林 |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DOI | 10.3969/j.issn.1001-5256.2013.11.015 |
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| DocumentTitleAlternate | Effects of silencing glypican -3 gene transcription on proliferation and apoptosis of hepatoma cells |
| DocumentTitle_FL | Effects of silencing glypican-3 gene transcription on proliferation and apoptosis of hepatoma cells |
| EndPage | 866 |
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| Issue | 11 |
| Keywords | 细胞凋亡 基因沉默 癌,肝细胞 磷脂酰肌醇蛋白聚糖类 细胞增殖 |
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| Notes | TAI Bojun, YAO Min, GU Xing, et al. （ Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nantong 226001, China） Objective To investigate the effects of silencing glypican -3 （GPC -3） gene transcription by shRNA on the proliferation and apoptosis of hepatoma cells. Methods GPC - 3 - shRNA was inserted into pGPU6/GFP/Neo vector, and HepG2 cells were transfected with the vector. The mRNA and protein expression of GPC -3 was measured by fluorescence quantitative PCR and Western blot; the proliferation of HepG2 cells was evaluated by MTT assay; the cell cycle and apoptosis of HepG2 cells were analyzed by flow cytometry, Annexin -V -PE/7 -AAD staining, and apoptotic DNA ladder kit. Results After the HepG2 cells were transfected with GPC -3 -shRNA, the GPC -3 mRNA silencing rate was up to 89.3%, in accordance with the down - regulation of GPC - 3 protein ; the proliferation inhibition rate of HepG2 cells was as high as 71.1% ; the HepG2 cells were arrested in GI phase, and the apoptosis |
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| PublicationTitle | 临床肝胆病杂志 |
| PublicationTitleAlternate | Chinese Journal of Clinical Hepatology |
| PublicationTitle_FL | JOURNAL OF CLINICAL HEPATOLOGY |
| PublicationYear | 2013 |
| Publisher | 南通大学附属医院,临床医学研究中心,江苏,南通,226001 |
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| Snippet | 目的探讨shRNA干预磷脂酰肌醇蛋白多糖（GPC）-3基因转录对肝癌细胞增殖和凋亡的影响。方法将GPC-3-shRNA插入pGPU6／GFP／Neo质粒，转染人肝癌tlepG2细胞，以Western Blot和... R735.7; 目的 探讨shRNA干预磷脂酰肌醇蛋白多糖(GPC)-3基因转录对肝癌细胞增殖和凋亡的影响.方法 将GPC-3-shRNA插入pGPU6/GFP/Neo质粒,转染人肝癌HepG2细胞,以Western... |
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| SubjectTerms | 基因沉默 癌 磷脂酰肌醇蛋白聚糖类 细胞凋亡 细胞增殖 肝细胞 |
| Title | 磷脂酰肌醇蛋白多糖-3对肝癌细胞增殖和凋亡的影响 |
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