利用间歇浸没式生物反应器进行甘蔗脱毒苗快繁研究

利用新型间歇浸没式生物反应系统（TIBs）在培养基配方、接种数量、蔗糖浓度及品种差异等方面对甘蔗脱毒组培苗快繁进行研究。结果表明：①PP333及6-BA＋PP333均能促进组培苗分化,增殖率较高且生长整齐,可进入下一阶段培养;②GA3浓度为1.0mg·L^-1伸长培养效果最好,平均苗高9.87cm;③每升培养液接种量为20株时的增殖率最高;④培养基蔗糖浓度以20g·L^-1较为适宜。利用该系统对ROC16和ROC22两个甘蔗品种的脱毒苗经过3个阶段的培养,ROC16增殖率达40倍,每瓶得苗800株苗左右;ROC22增殖率为30倍,每瓶苗的数量达600株。...

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Published inXinan nongye xuebao Vol. 22; no. 4; pp. 1038 - 1041
Main Author 刘丽敏 谭裕模 李松 唐红琴 刘红坚 余坤兴 淡明 李翔 戴友铭
Format Journal Article
LanguageChinese
Published 广西甘蔗研究所,广西,南宁,530007%中国农科院甘蔗研究中心,广西,南宁,530007 2009
Subjects
培养基
甘蔗
组培快繁
脱毒苗
间歇浸没式生物反应系统（TIBs）
甘蔗
脱毒苗
培养基
组培快繁
间歇浸没式生物反应系统(TIBs)
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ISSN1001-4829
DOI10.3969/j.issn.1001-4829.2009.04.035

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Abstract 利用新型间歇浸没式生物反应系统（TIBs）在培养基配方、接种数量、蔗糖浓度及品种差异等方面对甘蔗脱毒组培苗快繁进行研究。结果表明：①PP333及6-BA＋PP333均能促进组培苗分化,增殖率较高且生长整齐,可进入下一阶段培养;②GA3浓度为1.0mg·L^-1伸长培养效果最好,平均苗高9.87cm;③每升培养液接种量为20株时的增殖率最高;④培养基蔗糖浓度以20g·L^-1较为适宜。利用该系统对ROC16和ROC22两个甘蔗品种的脱毒苗经过3个阶段的培养,ROC16增殖率达40倍,每瓶得苗800株苗左右;ROC22增殖率为30倍,每瓶苗的数量达600株。
AbstractList 利用新型间歇浸没式生物反应系统（TIBs）在培养基配方、接种数量、蔗糖浓度及品种差异等方面对甘蔗脱毒组培苗快繁进行研究。结果表明：①PP333及6-BA＋PP333均能促进组培苗分化,增殖率较高且生长整齐,可进入下一阶段培养;②GA3浓度为1.0mg·L^-1伸长培养效果最好,平均苗高9.87cm;③每升培养液接种量为20株时的增殖率最高;④培养基蔗糖浓度以20g·L^-1较为适宜。利用该系统对ROC16和ROC22两个甘蔗品种的脱毒苗经过3个阶段的培养,ROC16增殖率达40倍,每瓶得苗800株苗左右;ROC22增殖率为30倍,每瓶苗的数量达600株。
利用新型间歇浸没式生物反应系统(TIBs)在培养基配方、接种数量、蔗糖浓度及品种差异等方面对甘蔗脱毒组培苗快繁进行研究.结果表明:①PP333及6-BA+PP333均能促进组培苗分化,增殖率较高且生长整齐,可进入下一阶段培养;②GA3浓度为1.0 mg·L-1伸长培养效果最好,平均苗高9.87 cm;③每升培养液接种量为20株时的增殖率最高;④培养基蔗糖浓度以20 g·L-1较为适宜.利用该系统对ROC16和ROC22两个甘蔗品种的脱毒苗经过3个阶段的培养,ROC16增殖率达40倍,每瓶得苗800株苗左右;ROC22增殖率为30倍,每瓶苗的数量达600株.
Author 刘丽敏 谭裕模 李松 唐红琴 刘红坚 余坤兴 淡明 李翔 戴友铭
AuthorAffiliation 广西甘蔗研究所,广西南宁530007 中国农科院甘蔗研究中心,广西南宁530007
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Author_FL LIU Li-min
TANG Hong-qin
DAN Ming
LI Xiang
DAI You-ming
LIU Hong-jian
TAN Yu-mo
YU Kun-xing
LI Song
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Keywords 甘蔗
脱毒苗
培养基
组培快繁
间歇浸没式生物反应系统(TIBs)
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Snippet 利用新型间歇浸没式生物反应系统（TIBs）在培养基配方、接种数量、蔗糖浓度及品种差异等方面对甘蔗脱毒组培苗快繁进行研究。结果表明：①PP333及6-BA＋PP333均能促进组培苗分...
利用新型间歇浸没式生物反应系统(TIBs)在培养基配方、接种数量、蔗糖浓度及品种差异等方面对甘蔗脱毒组培苗快繁进行研究.结果表明:①PP333及6-BA+PP333均能促进组培苗分化,增...
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SubjectTerms 培养基
甘蔗
组培快繁
脱毒苗
间歇浸没式生物反应系统（TIBs）
Title 利用间歇浸没式生物反应器进行甘蔗脱毒苗快繁研究
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