Modular Protein Expression Toolbox (MoPET), a standardized assembly system for defined expression constructs and expression optimization libraries - Weber, Ernst; Birkenfeld, Jörg; Franz, Jürgen; Gritzan, Uwe; Linden, Lars; Trautwein, Mark (PloS one, 2017)
Keywords: Adaptation; Adaptations; Algorithms; Amino acids; Assaying; Assembly; Attitude control; Backbone; Biochemistry; Biological activity; Biological products; Biology; Biology and Life Sciences; Biotin; Biotinylation; Cellular proteins; Cloning; Cloning vectors; Cloning, Molecular - methods; Coding; Combinatorial analysis; Constrictions; Deoxyribonucleic acid; Design; DNA; DNA shuffling; E coli; Effectors; Endonuclease; Engineering; Fragmentation; Gene Library; Genes; Genetic Vectors - genetics; HEK293 Cells; Homologous recombination; Homology; Humans; Medical screening; Methods; Modules; Molecular biology; Nucleic acids; Nucleotide sequence; Nucleotides; Optimization; Peptides; Physical Sciences; Polyadenylation; Polypeptides; Promoters; Properties; Protein engineering; Protein Engineering - methods; Protein expression; Protein synthesis; Proteins; Proteomics; Pyridine nucleotide reductase; Recombinant DNA; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Research and Analysis Methods; Reviews; Signal peptides; Standardization; Terminal protein; Tracking; Transcription factors; Transfection; Viruses; Germany