Breadth of CD8 T-cell mediated inhibition of replication of diverse HIV-1 transmitted-founder isolates correlates with the breadth of recognition within a comprehensive HIV-1 Gag, Nef, Env and Pol potential T-cell epitope (PTE) peptide set

Full characterisation of functional HIV-1-specific T-cell responses, including identification of recognised epitopes linked with functional antiviral responses, would aid development of effective vaccines but is hampered by HIV-1 sequence diversity. Typical approaches to identify T-cell epitopes uti...

Full description

Saved in:
Bibliographic Details
Published inPloS one Vol. 16; no. 11; p. e0260118
Main Authors Hayes, Peter, Fernandez, Natalia, Ochsenbauer, Christina, Dalel, Jama, Hare, Jonathan, King, Deborah, Black, Lucas, Streatfield, Claire, Kakarla, Vanaja, Macharia, Gladys, Makinde, Julia, Price, Matt, Hunter, Eric, Gilmour, Jill
Format Journal Article
LanguageEnglish
Published United States Public Library of Science 17.11.2021
Public Library of Science (PLoS)
Subjects
Adult
Amino acids
Analysis
Antibodies
Antigenic determinants
Antiretroviral drugs
Antiviral drugs
Biology and Life Sciences
CD3 antigen
CD4 antigen
CD8 antigen
CD8-Positive T-Lymphocytes - immunology
Diagnosis
Enzyme-linked immunosorbent assay
Epitopes
Epitopes, T-Lymphocyte - immunology
Female
gag Gene Products, Human Immunodeficiency Virus - immunology
Gag protein
Health aspects
HIV
HIV (Viruses)
HIV Infections - immunology
HIV Infections - virology
HIV-1 - immunology
Human immunodeficiency virus
Humans
Immunology
Infections
Laboratories
Lymphocytes T
Male
Medicine and Health Sciences
nef Gene Products, Human Immunodeficiency Virus - immunology
Peptides
Peptides - immunology
Peripheral blood mononuclear cells
Properties
Recognition
Replication
Research and Analysis Methods
Supervision
T cells
Vaccines
Virus Replication - drug effects
γ-Interferon
United States
New York
San Francisco California
California
United Kingdom > UK
United States > US
Online AccessGet full text
ISSN1932-6203
1932-6203
DOI10.1371/journal.pone.0260118

Cover

Abstract Full characterisation of functional HIV-1-specific T-cell responses, including identification of recognised epitopes linked with functional antiviral responses, would aid development of effective vaccines but is hampered by HIV-1 sequence diversity. Typical approaches to identify T-cell epitopes utilising extensive peptide sets require subjects’ cell numbers that exceed feasible sample volumes. To address this, CD8 T-cells were polyclonally expanded from PBMC from 13 anti-retroviral naïve subjects living with HIV using CD3/CD4 bi-specific antibody. Assessment of recognition of individual peptides within a set of 1408 HIV-1 Gag, Nef, Pol and Env potential T-cell epitope peptides was achieved by sequential IFNγ ELISpot assays using peptides pooled in 3-D matrices followed by confirmation with single peptides. A Renilla reniformis luciferase viral inhibition assay assessed CD8 T-cell-mediated inhibition of replication of a cross-clade panel of 10 HIV-1 isolates, including 9 transmitted-founder isolates. Polyclonal expansion from one frozen PBMC vial provided sufficient CD8 T-cells for both ELISpot steps in 12 of 13 subjects. A median of 33 peptides in 16 epitope regions were recognised including peptides located in previously characterised HIV-1 epitope-rich regions. There was no significant difference between ELISpot magnitudes for in vitro expanded CD8 T-cells and CD8 T-cells directly isolated from PBMCs. CD8 T-cells from all subjects inhibited a median of 7 HIV-1 isolates (range 4 to 10). The breadth of CD8 T-cell mediated HIV-1 inhibition was significantly positively correlated with CD8 T-cell breadth of peptide recognition. Polyclonal CD8 T-cell expansion allowed identification of HIV-1 isolates inhibited and peptides recognised within a large peptide set spanning the major HIV-1 proteins. This approach overcomes limitations associated with obtaining sufficient cell numbers to fully characterise HIV-1-specific CD8 T-cell responses by different functional readouts within the context of extreme HIV-1 diversity. Such an approach will have useful applications in clinical development for HIV-1 and other diseases.
AbstractList Full characterisation of functional HIV-1-specific T-cell responses, including identification of recognised epitopes linked with functional antiviral responses, would aid development of effective vaccines but is hampered by HIV-1 sequence diversity. Typical approaches to identify T-cell epitopes utilising extensive peptide sets require subjects’ cell numbers that exceed feasible sample volumes. To address this, CD8 T-cells were polyclonally expanded from PBMC from 13 anti-retroviral naïve subjects living with HIV using CD3/CD4 bi-specific antibody. Assessment of recognition of individual peptides within a set of 1408 HIV-1 Gag, Nef, Pol and Env potential T-cell epitope peptides was achieved by sequential IFNγ ELISpot assays using peptides pooled in 3-D matrices followed by confirmation with single peptides. A Renilla reniformis luciferase viral inhibition assay assessed CD8 T-cell-mediated inhibition of replication of a cross-clade panel of 10 HIV-1 isolates, including 9 transmitted-founder isolates. Polyclonal expansion from one frozen PBMC vial provided sufficient CD8 T-cells for both ELISpot steps in 12 of 13 subjects. A median of 33 peptides in 16 epitope regions were recognised including peptides located in previously characterised HIV-1 epitope-rich regions. There was no significant difference between ELISpot magnitudes for in vitro expanded CD8 T-cells and CD8 T-cells directly isolated from PBMCs. CD8 T-cells from all subjects inhibited a median of 7 HIV-1 isolates (range 4 to 10). The breadth of CD8 T-cell mediated HIV-1 inhibition was significantly positively correlated with CD8 T-cell breadth of peptide recognition. Polyclonal CD8 T-cell expansion allowed identification of HIV-1 isolates inhibited and peptides recognised within a large peptide set spanning the major HIV-1 proteins. This approach overcomes limitations associated with obtaining sufficient cell numbers to fully characterise HIV-1-specific CD8 T-cell responses by different functional readouts within the context of extreme HIV-1 diversity. Such an approach will have useful applications in clinical development for HIV-1 and other diseases.
Full characterisation of functional HIV-1-specific T-cell responses, including identification of recognised epitopes linked with functional antiviral responses, would aid development of effective vaccines but is hampered by HIV-1 sequence diversity. Typical approaches to identify T-cell epitopes utilising extensive peptide sets require subjects’ cell numbers that exceed feasible sample volumes. To address this, CD8 T-cells were polyclonally expanded from PBMC from 13 anti-retroviral naïve subjects living with HIV using CD3/CD4 bi-specific antibody. Assessment of recognition of individual peptides within a set of 1408 HIV-1 Gag, Nef, Pol and Env potential T-cell epitope peptides was achieved by sequential IFNγ ELISpot assays using peptides pooled in 3-D matrices followed by confirmation with single peptides. A Renilla reniformis luciferase viral inhibition assay assessed CD8 T-cell-mediated inhibition of replication of a cross-clade panel of 10 HIV-1 isolates, including 9 transmitted-founder isolates. Polyclonal expansion from one frozen PBMC vial provided sufficient CD8 T-cells for both ELISpot steps in 12 of 13 subjects. A median of 33 peptides in 16 epitope regions were recognised including peptides located in previously characterised HIV-1 epitope-rich regions. There was no significant difference between ELISpot magnitudes for in vitro expanded CD8 T-cells and CD8 T-cells directly isolated from PBMCs. CD8 T-cells from all subjects inhibited a median of 7 HIV-1 isolates (range 4 to 10). The breadth of CD8 T-cell mediated HIV-1 inhibition was significantly positively correlated with CD8 T-cell breadth of peptide recognition. Polyclonal CD8 T-cell expansion allowed identification of HIV-1 isolates inhibited and peptides recognised within a large peptide set spanning the major HIV-1 proteins. This approach overcomes limitations associated with obtaining sufficient cell numbers to fully characterise HIV-1-specific CD8 T-cell responses by different functional readouts within the context of extreme HIV-1 diversity. Such an approach will have useful applications in clinical development for HIV-1 and other diseases.
Full characterisation of functional HIV-1-specific T-cell responses, including identification of recognised epitopes linked with functional antiviral responses, would aid development of effective vaccines but is hampered by HIV-1 sequence diversity. Typical approaches to identify T-cell epitopes utilising extensive peptide sets require subjects' cell numbers that exceed feasible sample volumes. To address this, CD8 T-cells were polyclonally expanded from PBMC from 13 anti-retroviral naïve subjects living with HIV using CD3/CD4 bi-specific antibody. Assessment of recognition of individual peptides within a set of 1408 HIV-1 Gag, Nef, Pol and Env potential T-cell epitope peptides was achieved by sequential IFNγ ELISpot assays using peptides pooled in 3-D matrices followed by confirmation with single peptides. A Renilla reniformis luciferase viral inhibition assay assessed CD8 T-cell-mediated inhibition of replication of a cross-clade panel of 10 HIV-1 isolates, including 9 transmitted-founder isolates. Polyclonal expansion from one frozen PBMC vial provided sufficient CD8 T-cells for both ELISpot steps in 12 of 13 subjects. A median of 33 peptides in 16 epitope regions were recognised including peptides located in previously characterised HIV-1 epitope-rich regions. There was no significant difference between ELISpot magnitudes for in vitro expanded CD8 T-cells and CD8 T-cells directly isolated from PBMCs. CD8 T-cells from all subjects inhibited a median of 7 HIV-1 isolates (range 4 to 10). The breadth of CD8 T-cell mediated HIV-1 inhibition was significantly positively correlated with CD8 T-cell breadth of peptide recognition. Polyclonal CD8 T-cell expansion allowed identification of HIV-1 isolates inhibited and peptides recognised within a large peptide set spanning the major HIV-1 proteins. This approach overcomes limitations associated with obtaining sufficient cell numbers to fully characterise HIV-1-specific CD8 T-cell responses by different functional readouts within the context of extreme HIV-1 diversity. Such an approach will have useful applications in clinical development for HIV-1 and other diseases.Full characterisation of functional HIV-1-specific T-cell responses, including identification of recognised epitopes linked with functional antiviral responses, would aid development of effective vaccines but is hampered by HIV-1 sequence diversity. Typical approaches to identify T-cell epitopes utilising extensive peptide sets require subjects' cell numbers that exceed feasible sample volumes. To address this, CD8 T-cells were polyclonally expanded from PBMC from 13 anti-retroviral naïve subjects living with HIV using CD3/CD4 bi-specific antibody. Assessment of recognition of individual peptides within a set of 1408 HIV-1 Gag, Nef, Pol and Env potential T-cell epitope peptides was achieved by sequential IFNγ ELISpot assays using peptides pooled in 3-D matrices followed by confirmation with single peptides. A Renilla reniformis luciferase viral inhibition assay assessed CD8 T-cell-mediated inhibition of replication of a cross-clade panel of 10 HIV-1 isolates, including 9 transmitted-founder isolates. Polyclonal expansion from one frozen PBMC vial provided sufficient CD8 T-cells for both ELISpot steps in 12 of 13 subjects. A median of 33 peptides in 16 epitope regions were recognised including peptides located in previously characterised HIV-1 epitope-rich regions. There was no significant difference between ELISpot magnitudes for in vitro expanded CD8 T-cells and CD8 T-cells directly isolated from PBMCs. CD8 T-cells from all subjects inhibited a median of 7 HIV-1 isolates (range 4 to 10). The breadth of CD8 T-cell mediated HIV-1 inhibition was significantly positively correlated with CD8 T-cell breadth of peptide recognition. Polyclonal CD8 T-cell expansion allowed identification of HIV-1 isolates inhibited and peptides recognised within a large peptide set spanning the major HIV-1 proteins. This approach overcomes limitations associated with obtaining sufficient cell numbers to fully characterise HIV-1-specific CD8 T-cell responses by different functional readouts within the context of extreme HIV-1 diversity. Such an approach will have useful applications in clinical development for HIV-1 and other diseases.
Full characterisation of functional HIV-1-specific T-cell responses, including identification of recognised epitopes linked with functional antiviral responses, would aid development of effective vaccines but is hampered by HIV-1 sequence diversity. Typical approaches to identify T-cell epitopes utilising extensive peptide sets require subjects' cell numbers that exceed feasible sample volumes. To address this, CD8 T-cells were polyclonally expanded from PBMC from 13 anti-retroviral naïve subjects living with HIV using CD3/CD4 bi-specific antibody. Assessment of recognition of individual peptides within a set of 1408 HIV-1 Gag, Nef, Pol and Env potential T-cell epitope peptides was achieved by sequential IFN[gamma] ELISpot assays using peptides pooled in 3-D matrices followed by confirmation with single peptides. A Renilla reniformis luciferase viral inhibition assay assessed CD8 T-cell-mediated inhibition of replication of a cross-clade panel of 10 HIV-1 isolates, including 9 transmitted-founder isolates. Polyclonal expansion from one frozen PBMC vial provided sufficient CD8 T-cells for both ELISpot steps in 12 of 13 subjects. A median of 33 peptides in 16 epitope regions were recognised including peptides located in previously characterised HIV-1 epitope-rich regions. There was no significant difference between ELISpot magnitudes for in vitro expanded CD8 T-cells and CD8 T-cells directly isolated from PBMCs. CD8 T-cells from all subjects inhibited a median of 7 HIV-1 isolates (range 4 to 10). The breadth of CD8 T-cell mediated HIV-1 inhibition was significantly positively correlated with CD8 T-cell breadth of peptide recognition. Polyclonal CD8 T-cell expansion allowed identification of HIV-1 isolates inhibited and peptides recognised within a large peptide set spanning the major HIV-1 proteins. This approach overcomes limitations associated with obtaining sufficient cell numbers to fully characterise HIV-1-specific CD8 T-cell responses by different functional readouts within the context of extreme HIV-1 diversity. Such an approach will have useful applications in clinical development for HIV-1 and other diseases.
Audience Academic
Author Fernandez, Natalia
Macharia, Gladys
Kakarla, Vanaja
Makinde, Julia
Hare, Jonathan
Dalel, Jama
Ochsenbauer, Christina
King, Deborah
Hayes, Peter
Streatfield, Claire
Price, Matt
Gilmour, Jill
Hunter, Eric
Black, Lucas
AuthorAffiliation 4 Department of Epidemiology and Biostatistics, University of California at San Francisco, San Francisco, California, United States of America
2 University of Alabama, Birmingham, Alabama, United States of America
3 IAVI, New York, New York, United States of America
1 IAVI Human Immunology Laboratory, Imperial College, London, United Kingdom
Cornell University Joan and Sanford I Weill Medical College, UNITED STATES
5 Emory Vaccine Center, Atlanta, Georgia, United States of America
AuthorAffiliation_xml – name: 5 Emory Vaccine Center, Atlanta, Georgia, United States of America
– name: Cornell University Joan and Sanford I Weill Medical College, UNITED STATES
– name: 3 IAVI, New York, New York, United States of America
– name: 1 IAVI Human Immunology Laboratory, Imperial College, London, United Kingdom
– name: 2 University of Alabama, Birmingham, Alabama, United States of America
– name: 4 Department of Epidemiology and Biostatistics, University of California at San Francisco, San Francisco, California, United States of America
Author_xml – sequence: 1
  givenname: Peter
  orcidid: 0000-0002-1251-934X
  surname: Hayes
  fullname: Hayes, Peter
– sequence: 2
  givenname: Natalia
  surname: Fernandez
  fullname: Fernandez, Natalia
– sequence: 3
  givenname: Christina
  orcidid: 0000-0003-1166-5879
  surname: Ochsenbauer
  fullname: Ochsenbauer, Christina
– sequence: 4
  givenname: Jama
  surname: Dalel
  fullname: Dalel, Jama
– sequence: 5
  givenname: Jonathan
  surname: Hare
  fullname: Hare, Jonathan
– sequence: 6
  givenname: Deborah
  surname: King
  fullname: King, Deborah
– sequence: 7
  givenname: Lucas
  orcidid: 0000-0002-4463-6911
  surname: Black
  fullname: Black, Lucas
– sequence: 8
  givenname: Claire
  surname: Streatfield
  fullname: Streatfield, Claire
– sequence: 9
  givenname: Vanaja
  surname: Kakarla
  fullname: Kakarla, Vanaja
– sequence: 10
  givenname: Gladys
  orcidid: 0000-0002-5605-5604
  surname: Macharia
  fullname: Macharia, Gladys
– sequence: 11
  givenname: Julia
  surname: Makinde
  fullname: Makinde, Julia
– sequence: 12
  givenname: Matt
  surname: Price
  fullname: Price, Matt
– sequence: 13
  givenname: Eric
  surname: Hunter
  fullname: Hunter, Eric
– sequence: 14
  givenname: Jill
  surname: Gilmour
  fullname: Gilmour, Jill
BackLink https://www.ncbi.nlm.nih.gov/pubmed/34788349$$D View this record in MEDLINE/PubMed
BookMark eNp9U81u1DAQjlAR_YE3QGCJS5GaxYkdx-GAVEppK1XQQ-FqOc5411XWDra3iKfmFXB2s9BWFcoh9vj7mRl79rMd6yxk2csCzwpSF-9u3Mpb2c-GFJ7hkuGi4E-yvaIhZc5KTHburHez_RBuMK4IZ-xZtktozTmhzV72-6MH2cUFchqdfOLoOlfQ92gJnZEROmTswrQmGmdHhIehN0put525BR8AnV98zwsUvbRhaWKi5dqtbAcemeD6pBOQct7DZvnTJLu4ANT-s_ag3NxufMZzY5FMnOXgYQE2JJ_J5EzOj9AX0Efo1N4iaTt05Xo0uAg2Gtlv84fBRDcAOry6Pn2LBhii6QAFiM-zp1r2AV5M_4Ps2-fT65Pz_PLr2cXJ8WWuqobEnEpdyYa1hDe4K6CteMNKUmCqQUnVcSBVS0nXYKhprbGqGGdNA5Ro6LCuMTnIXm90h94FMd1VEGXV8ArXrKwT4mKD6Jy8EYM3S-l_CSeNWAecnwvpo1E9CK6VrDivqNYVpRRz1UpWlqTiFJdFqZPWh8lt1aarU6kZXvb3RO-fWLMQc3creMoHFzwJHE4C3v1YQYhiacLYSWnBrdZ5N5g1jI55v3kAfby6CTWXqQBjtUu-ahQVx4yXvGY1G7s0ewSVvg6WRqWHrU2K3yO8ulvo3wq3DzoB3m8AyrsQPGihTFw_2KRselFgMU7PNmcxTo-YpieR6QPyVv-_tD-oWSKI
CitedBy_id crossref_primary_10_1016_j_drudis_2022_103458
crossref_primary_10_1016_j_ebiom_2024_104987
crossref_primary_10_1016_j_lanmic_2024_100956
crossref_primary_10_1097_COH_0000000000000815
crossref_primary_10_3390_vaccines11020472
crossref_primary_10_1097_COH_0000000000000765
crossref_primary_10_3389_fimmu_2022_1029029
Cites_doi 10.1093/ije/dyaa100
10.1038/s41591-020-0858-8
10.1093/bmb/58.1.19
10.1126/science.1070441
10.1128/JVI.00110-09
10.1128/JVI.77.3.2081-2092.2003
10.3389/fimmu.2021.634832
10.1111/imr.12484
10.1371/journal.pone.0064405
10.1097/QAD.0000000000001122
10.1128/JVI.77.13.7330-7340.2003
10.1016/j.virol.2010.08.028
10.1016/j.immuni.2010.09.011
10.1016/0090-1229(91)90139-2
10.1016/j.vaccine.2006.06.009
10.1084/jem.20090365
10.1016/j.jim.2021.112970
10.1093/molbev/msz312
10.1182/blood-2005-12-4818
10.1371/journal.ppat.1001028
10.1016/j.chom.2012.09.008
10.1038/s41577-020-0274-9
10.1128/JVI.02564-06
10.1016/S0022-1759(02)00423-4
10.1084/jem.20090378
10.1371/journal.pone.0014330
10.1007/s00239-001-2304-y
10.1371/journal.pone.0090378
10.2172/1458915
10.1002/cyto.a.20646
10.1089/aid.2015.0074
10.1128/JVI.00138-10
10.1371/journal.pone.0030963
10.1128/CVI.00326-08
10.1016/j.jim.2013.11.021
10.1016/j.ebiom.2020.102682
10.1093/molbev/msy096
10.1128/JVI.06157-11
10.1128/JVI.00484-18
10.1126/science.299.5612.1515c
10.1086/650492
10.1371/journal.pone.0147812
ContentType Journal Article
Copyright COPYRIGHT 2021 Public Library of Science
2021 Hayes et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
2021 Hayes et al 2021 Hayes et al
Copyright_xml – notice: COPYRIGHT 2021 Public Library of Science
– notice: 2021 Hayes et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
– notice: 2021 Hayes et al 2021 Hayes et al
CorporateAuthor The IAVI protocol C investigators
IAVI protocol C investigators
CorporateAuthor_xml – name: The IAVI protocol C investigators
– name: IAVI protocol C investigators
DBID AAYXX
CITATION
CGR
CUY
CVF
ECM
EIF
NPM
3V.
7QG
7QL
7QO
7RV
7SN
7SS
7T5
7TG
7TM
7U9
7X2
7X7
7XB
88E
8AO
8C1
8FD
8FE
8FG
8FH
8FI
8FJ
8FK
ABJCF
ABUWG
AEUYN
AFKRA
ARAPS
ATCPS
AZQEC
BBNVY
BENPR
BGLVJ
BHPHI
C1K
CCPQU
D1I
DWQXO
FR3
FYUFA
GHDGH
GNUQQ
H94
HCIFZ
K9.
KB.
KB0
KL.
L6V
LK8
M0K
M0S
M1P
M7N
M7P
M7S
NAPCQ
P5Z
P62
P64
PATMY
PDBOC
PHGZM
PHGZT
PIMPY
PJZUB
PKEHL
PPXIY
PQEST
PQGLB
PQQKQ
PQUKI
PRINS
PTHSS
PYCSY
RC3
7X8
5PM
DOA
DOI 10.1371/journal.pone.0260118
DatabaseName CrossRef
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
ProQuest Central (Corporate)
Animal Behavior Abstracts
Bacteriology Abstracts (Microbiology B)
Biotechnology Research Abstracts
ProQuest Nursing and Allied Health Journals - PSU access expires 11/30/25.
Ecology Abstracts
Entomology Abstracts (Full archive)
Immunology Abstracts
Meteorological & Geoastrophysical Abstracts
Nucleic Acids Abstracts
Virology and AIDS Abstracts
Agricultural Science Collection
Health & Medical Collection
ProQuest Central (purchase pre-March 2016)
Medical Database (Alumni Edition)
ProQuest Pharma Collection
Public Health Database
Technology Research Database
ProQuest SciTech Collection
ProQuest Technology Collection
ProQuest Natural Science Collection
Hospital Premium Collection
Hospital Premium Collection (Alumni Edition)
ProQuest Central (Alumni) (purchase pre-March 2016)
Materials Science & Engineering Collection
ProQuest Central (Alumni)
ProQuest One Sustainability
ProQuest Central UK/Ireland
Advanced Technologies & Aerospace Collection
Agricultural & Environmental Science Collection
ProQuest Central Essentials
Biological Science Collection
ProQuest Central
Technology collection
Natural Science Collection
Environmental Sciences and Pollution Management
ProQuest One Community College
ProQuest Materials Science Collection
ProQuest Central
Engineering Research Database
Health Research Premium Collection
Health Research Premium Collection (Alumni)
ProQuest Central Student
AIDS and Cancer Research Abstracts
SciTech Premium Collection
ProQuest Health & Medical Complete (Alumni)
Materials Science Database
Nursing & Allied Health Database (Alumni Edition)
Meteorological & Geoastrophysical Abstracts - Academic
ProQuest Engineering Collection
Biological Sciences
Agriculture Science Database
ProQuest Health & Medical Collection
Medical Database
Algology Mycology and Protozoology Abstracts (Microbiology C)
Biological Science Database
Engineering Database
Nursing & Allied Health Premium
Advanced Technologies & Aerospace Database
ProQuest Advanced Technologies & Aerospace Collection
Biotechnology and BioEngineering Abstracts
Environmental Science Database
Materials Science Collection
ProQuest One Academic
ProQuest One Academic (New)
Publicly Available Content Database
ProQuest Health & Medical Research Collection
ProQuest One Academic Middle East (New)
ProQuest One Health & Nursing
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Applied & Life Sciences
ProQuest One Academic
ProQuest One Academic UKI Edition
ProQuest Central China
Engineering Collection
Environmental Science Collection
Genetics Abstracts
MEDLINE - Academic
PubMed Central (Full Participant titles)
DOAJ Open Access Full Text
DatabaseTitle CrossRef
MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
Agricultural Science Database
Publicly Available Content Database
ProQuest Central Student
ProQuest Advanced Technologies & Aerospace Collection
ProQuest Central Essentials
Nucleic Acids Abstracts
SciTech Premium Collection
ProQuest Central China
Environmental Sciences and Pollution Management
ProQuest One Applied & Life Sciences
ProQuest One Sustainability
Health Research Premium Collection
Meteorological & Geoastrophysical Abstracts
Natural Science Collection
Health & Medical Research Collection
Biological Science Collection
ProQuest Central (New)
ProQuest Medical Library (Alumni)
Engineering Collection
Advanced Technologies & Aerospace Collection
Engineering Database
Virology and AIDS Abstracts
ProQuest Biological Science Collection
ProQuest One Academic Eastern Edition
Agricultural Science Collection
ProQuest Hospital Collection
ProQuest Technology Collection
Health Research Premium Collection (Alumni)
Biological Science Database
Ecology Abstracts
ProQuest Hospital Collection (Alumni)
Biotechnology and BioEngineering Abstracts
Environmental Science Collection
Entomology Abstracts
Nursing & Allied Health Premium
ProQuest Health & Medical Complete
ProQuest One Academic UKI Edition
Environmental Science Database
ProQuest Nursing & Allied Health Source (Alumni)
Engineering Research Database
ProQuest One Academic
Meteorological & Geoastrophysical Abstracts - Academic
ProQuest One Academic (New)
Technology Collection
Technology Research Database
ProQuest One Academic Middle East (New)
Materials Science Collection
ProQuest Health & Medical Complete (Alumni)
ProQuest Central (Alumni Edition)
ProQuest One Community College
ProQuest One Health & Nursing
ProQuest Natural Science Collection
ProQuest Pharma Collection
ProQuest Central
ProQuest Health & Medical Research Collection
Genetics Abstracts
ProQuest Engineering Collection
Biotechnology Research Abstracts
Health and Medicine Complete (Alumni Edition)
ProQuest Central Korea
Bacteriology Abstracts (Microbiology B)
Algology Mycology and Protozoology Abstracts (Microbiology C)
Agricultural & Environmental Science Collection
AIDS and Cancer Research Abstracts
Materials Science Database
ProQuest Materials Science Collection
ProQuest Public Health
ProQuest Nursing & Allied Health Source
ProQuest SciTech Collection
Advanced Technologies & Aerospace Database
ProQuest Medical Library
Animal Behavior Abstracts
Materials Science & Engineering Collection
Immunology Abstracts
ProQuest Central (Alumni)
MEDLINE - Academic
DatabaseTitleList

MEDLINE - Academic

Agricultural Science Database

CrossRef
MEDLINE
Database_xml – sequence: 1
  dbid: DOA
  name: DOAJ Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
– sequence: 2
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 3
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
– sequence: 4
  dbid: 8FG
  name: ProQuest Technology Collection
  url: https://search.proquest.com/technologycollection1
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Sciences (General)
DocumentTitleAlternate CD8 T-cell mediated inhibition of HIV-1 replication correlates with HIV-1 epitope recognition
EISSN 1932-6203
ExternalDocumentID 2598507627
oai_doaj_org_article_8fca58854ff544408cba62235840212f
PMC8598018
A682876760
34788349
10_1371_journal_pone_0260118
Genre Research Support, U.S. Gov't, Non-P.H.S
Journal Article
GeographicLocations United States
New York
San Francisco California
California
United Kingdom--UK
United States--US
GeographicLocations_xml – name: United States
– name: New York
– name: San Francisco California
– name: California
– name: United Kingdom--UK
– name: United States--US
GrantInformation_xml – fundername: ;
– fundername: ;
  grantid: AID-OAA-A-16-00032
GroupedDBID ---
123
29O
2WC
53G
5VS
7RV
7X2
7X7
7XC
88E
8AO
8C1
8CJ
8FE
8FG
8FH
8FI
8FJ
A8Z
AAFWJ
AAUCC
AAWOE
AAYXX
ABDBF
ABIVO
ABJCF
ABUWG
ACGFO
ACIHN
ACIWK
ACPRK
ACUHS
ADBBV
AEAQA
AENEX
AEUYN
AFKRA
AFPKN
AFRAH
AHMBA
ALIPV
ALMA_UNASSIGNED_HOLDINGS
AOIJS
APEBS
ARAPS
ATCPS
BAWUL
BBNVY
BCNDV
BENPR
BGLVJ
BHPHI
BKEYQ
BPHCQ
BVXVI
BWKFM
CCPQU
CITATION
CS3
D1I
D1J
D1K
DIK
DU5
E3Z
EAP
EAS
EBD
EMOBN
ESX
EX3
F5P
FPL
FYUFA
GROUPED_DOAJ
GX1
HCIFZ
HH5
HMCUK
HYE
IAO
IEA
IGS
IHR
IHW
INH
INR
IOV
IPY
ISE
ISR
ITC
K6-
KB.
KQ8
L6V
LK5
LK8
M0K
M1P
M48
M7P
M7R
M7S
M~E
NAPCQ
O5R
O5S
OK1
OVT
P2P
P62
PATMY
PDBOC
PHGZM
PHGZT
PIMPY
PQQKQ
PROAC
PSQYO
PTHSS
PV9
PYCSY
RNS
RPM
RZL
SV3
TR2
UKHRP
WOQ
WOW
~02
~KM
3V.
ADRAZ
BBORY
CGR
CUY
CVF
ECM
EIF
IPNFZ
NPM
RIG
PMFND
7QG
7QL
7QO
7SN
7SS
7T5
7TG
7TM
7U9
7XB
8FD
8FK
AZQEC
C1K
DWQXO
ESTFP
FR3
GNUQQ
H94
K9.
KL.
M7N
P64
PJZUB
PKEHL
PPXIY
PQEST
PQGLB
PQUKI
PRINS
PUEGO
RC3
7X8
5PM
-
02
AAPBV
ABPTK
ADACO
BBAFP
KM
ID FETCH-LOGICAL-c593t-4af5a96b3890d1eb589623104fecacd8e35b43d90e747f0c568699e43fed0f703
IEDL.DBID M48
ISSN 1932-6203
IngestDate Sun Dec 05 00:11:30 EST 2021
Wed Aug 27 01:07:53 EDT 2025
Thu Aug 21 14:14:15 EDT 2025
Fri Sep 05 14:44:57 EDT 2025
Sun Sep 07 05:31:10 EDT 2025
Tue Jun 17 21:17:36 EDT 2025
Tue Jun 10 20:16:26 EDT 2025
Wed Feb 19 02:09:16 EST 2025
Tue Jul 01 01:49:55 EDT 2025
Thu Apr 24 23:00:44 EDT 2025
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 11
Language English
License This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Creative Commons Attribution License
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c593t-4af5a96b3890d1eb589623104fecacd8e35b43d90e747f0c568699e43fed0f703
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
Membership of the IAVI protocol C investigators is listed in the Acknowledgments.
Competing Interests: The authors have declared that no competing interests exist.
ORCID 0000-0003-1166-5879
0000-0002-4463-6911
0000-0002-5605-5604
0000-0002-1251-934X
OpenAccessLink http://journals.scholarsportal.info/openUrl.xqy?doi=10.1371/journal.pone.0260118
PMID 34788349
PQID 2598507627
PQPubID 1436336
ParticipantIDs plos_journals_2598507627
doaj_primary_oai_doaj_org_article_8fca58854ff544408cba62235840212f
pubmedcentral_primary_oai_pubmedcentral_nih_gov_8598018
proquest_miscellaneous_2599069647
proquest_journals_2598507627
gale_infotracmisc_A682876760
gale_infotracacademiconefile_A682876760
pubmed_primary_34788349
crossref_citationtrail_10_1371_journal_pone_0260118
crossref_primary_10_1371_journal_pone_0260118
ProviderPackageCode CITATION
AAYXX
PublicationCentury 2000
PublicationDate 2021-11-17
PublicationDateYYYYMMDD 2021-11-17
PublicationDate_xml – month: 11
  year: 2021
  text: 2021-11-17
  day: 17
PublicationDecade 2020
PublicationPlace United States
PublicationPlace_xml – name: United States
– name: San Francisco
– name: San Francisco, CA USA
PublicationTitle PloS one
PublicationTitleAlternate PLoS One
PublicationYear 2021
Publisher Public Library of Science
Public Library of Science (PLoS)
Publisher_xml – name: Public Library of Science
– name: Public Library of Science (PLoS)
References U Malhotra (pone.0260118.ref012) 2007; 81
MJ McElrath (pone.0260118.ref001) 2010; 33
SA Freel (pone.0260118.ref040) 2010; 84
PJ Hayes (pone.0260118.ref019) 2016; 30
M Price (pone.0260118.ref026) 2021; 50
PS Arunachalam (pone.0260118.ref003) 2020; 26
J Kopycinski (pone.0260118.ref018) 2014; 9
MR Betts (pone.0260118.ref005) 2006; 107
N Goonetilleke (pone.0260118.ref006) 2009; 206
M Roederer (pone.0260118.ref013) 2003; 274
LM Walker (pone.0260118.ref042) 2010; 6
DC Nickle (pone.0260118.ref009) 2003; 299
MJ Gartner (pone.0260118.ref039) 2020; 53
MO Alberti (pone.0260118.ref022) 2015; 31
pone.0260118.ref029
JF Salazar-Gonzalez (pone.0260118.ref025) 2009; 206
JT Wong (pone.0260118.ref016) 1991; 58
MM Addo (pone.0260118.ref036) 2003; 77
C Ochsenbauer (pone.0260118.ref021) 2012; 86
MW Dimmic (pone.0260118.ref034) 2002; 55
C Michelo (pone.0260118.ref024) 2021; 491
LE McCoy (pone.0260118.ref043) 2017; 275
DK Gill (pone.0260118.ref028) 2010; 5
P Kunwar (pone.0260118.ref037) 2013; 8
A Fiore-Gartland (pone.0260118.ref015) 2016; 11
DR Burton (pone.0260118.ref002) 2012; 12
B Gaschen (pone.0260118.ref008) 2002; 296
S Kumar (pone.0260118.ref032) 2018; 35
MD Simek (pone.0260118.ref041) 2009; 83
DR Collins (pone.0260118.ref004) 2020; 20
J Makinde (pone.0260118.ref030) 2021; 12
M Altfeld (pone.0260118.ref010) 2003; 77
MA Naarding (pone.0260118.ref020) 2014; 409
pone.0260118.ref038
ML Precopio (pone.0260118.ref014) 2008; 73
A Spentzou (pone.0260118.ref017) 2010; 201
B Korber (pone.0260118.ref007) 2001; 58
J Prevost (pone.0260118.ref023) 2018; 92
G Stecher (pone.0260118.ref033) 2020; 37
F Li (pone.0260118.ref011) 2006; 24
TG Edmonds (pone.0260118.ref031) 2010; 408
MJ Boaz (pone.0260118.ref027) 2009; 16
DA Ozaki (pone.0260118.ref035) 2012; 7
References_xml – volume: 50
  start-page: 29
  year: 2021
  ident: pone.0260118.ref026
  article-title: Cohort Profile: IAVI’s HIV epidemiology and early infection cohort studies in Africa to support vaccine discovery
  publication-title: Int J Epidemiol
  doi: 10.1093/ije/dyaa100
– volume: 26
  start-page: 932
  year: 2020
  ident: pone.0260118.ref003
  article-title: T cell-inducing vaccine durably prevents mucosal SHIV infection even with lower neutralizing antibody titers
  publication-title: Nat Med
  doi: 10.1038/s41591-020-0858-8
– volume: 58
  start-page: 19
  year: 2001
  ident: pone.0260118.ref007
  article-title: Evolutionary and immunological implications of contemporary HIV-1 variation
  publication-title: Br Med Bull
  doi: 10.1093/bmb/58.1.19
– volume: 296
  start-page: 2354
  year: 2002
  ident: pone.0260118.ref008
  article-title: Diversity considerations in HIV-1 vaccine selection
  publication-title: Science
  doi: 10.1126/science.1070441
– volume: 83
  start-page: 7337
  year: 2009
  ident: pone.0260118.ref041
  article-title: Human immunodeficiency virus type 1 elite neutralizers: individuals with broad and potent neutralizing activity identified by using a high-throughput neutralization assay together with an analytical selection algorithm
  publication-title: Journal of Virology
  doi: 10.1128/JVI.00110-09
– volume: 77
  start-page: 2081
  year: 2003
  ident: pone.0260118.ref036
  article-title: Comprehensive epitope analysis of human immunodeficiency virus type 1 (HIV-1)-specific T-cell responses directed against the entire expressed HIV-1 genome demonstrate broadly directed responses, but no correlation to viral load
  publication-title: J Virol
  doi: 10.1128/JVI.77.3.2081-2092.2003
– volume: 12
  start-page: 634832
  year: 2021
  ident: pone.0260118.ref030
  article-title: A novel sample selection approach to aid the identification of factors that correlate with the control of HIV-1 infection
  publication-title: Front. Immunol
  doi: 10.3389/fimmu.2021.634832
– volume: 275
  start-page: 11
  year: 2017
  ident: pone.0260118.ref043
  article-title: Identification and specificity of broadly neutralizing antibodies against HIV
  publication-title: Immunol. Rev
  doi: 10.1111/imr.12484
– volume: 8
  start-page: e64405
  issue: 5
  year: 2013
  ident: pone.0260118.ref037
  article-title: Superior control of HIV-1 replication by CD8+ T cells targeting conserved epitopes: Implications for HIV vaccine design
  publication-title: PLoS ONE
  doi: 10.1371/journal.pone.0064405
– volume: 30
  start-page: 1703
  year: 2016
  ident: pone.0260118.ref019
  article-title: Adenovirus-based HIV-1 vaccine candidates tested in efficacy trials elicit CD8+ T cells with limited breadth of HIV-1 inhibition
  publication-title: Aids
  doi: 10.1097/QAD.0000000000001122
– volume: 77
  start-page: 7330
  year: 2003
  ident: pone.0260118.ref010
  article-title: Enhanced detection of human immunodeficiency virus type 1-specific T-cell responses to highly variable regions by using peptides based on autologous virus sequences
  publication-title: J Virol
  doi: 10.1128/JVI.77.13.7330-7340.2003
– volume: 408
  start-page: 1
  year: 2010
  ident: pone.0260118.ref031
  article-title: Replication competent molecular clones of HIV-1 expressing Renilla luciferase facilitate the analysis of antibody inhibition in PBMC
  publication-title: Virology
  doi: 10.1016/j.virol.2010.08.028
– volume: 33
  start-page: 542
  year: 2010
  ident: pone.0260118.ref001
  article-title: Induction of immunity to human immunodeficiency virus type-1 by vaccination
  publication-title: Immunity
  doi: 10.1016/j.immuni.2010.09.011
– volume: 58
  start-page: 236
  year: 1991
  ident: pone.0260118.ref016
  article-title: Selective reduction and proliferation of the CD4+ and CD8+ T cell subsets with bispecific monoclonal antibodies: evidence for inter-T cell-mediated cytolysis
  publication-title: Clin Immunol Immunopathol
  doi: 10.1016/0090-1229(91)90139-2
– volume: 24
  start-page: 6893
  year: 2006
  ident: pone.0260118.ref011
  article-title: Peptide selection for human immunodeficiency virus type 1 CTL-based vaccine evaluation
  publication-title: Vaccine
  doi: 10.1016/j.vaccine.2006.06.009
– volume: 206
  start-page: 1253
  year: 2009
  ident: pone.0260118.ref006
  article-title: The first T cell response to transmitted/founder virus contributes to the control of acute viremia in HIV-1 infection
  publication-title: J Exp Med
  doi: 10.1084/jem.20090365
– volume: 491
  start-page: 112970
  year: 2021
  ident: pone.0260118.ref024
  article-title: Comprehensive epitope mapping using polyclonally expanded human CD8 T cells and a two-step ELISpot assay for testing large peptide libraries
  publication-title: J Immunol Methods
  doi: 10.1016/j.jim.2021.112970
– volume: 37
  start-page: 1237
  year: 2020
  ident: pone.0260118.ref033
  article-title: Molecular Evolutionary Genetics Analysis (MEGA) for macOS
  publication-title: Molecular Biology and Evolution
  doi: 10.1093/molbev/msz312
– ident: pone.0260118.ref029
– volume: 107
  start-page: 4781
  year: 2006
  ident: pone.0260118.ref005
  article-title: HIV nonprogressors preferentially maintain highly functional HIV-specific CD8+ T cells
  publication-title: Blood
  doi: 10.1182/blood-2005-12-4818
– volume: 6
  start-page: e1001028
  year: 2010
  ident: pone.0260118.ref042
  article-title: A limited number of antibody specificities mediate broad and potent serum neutralization in selected HIV-1 infected individuals
  publication-title: PLoS Pathog
  doi: 10.1371/journal.ppat.1001028
– volume: 12
  start-page: 396
  year: 2012
  ident: pone.0260118.ref002
  article-title: A Blueprint for HIV Vaccine Discovery
  publication-title: Cell Host Microbe
  doi: 10.1016/j.chom.2012.09.008
– volume: 20
  start-page: 471
  year: 2020
  ident: pone.0260118.ref004
  article-title: CD8(+) T cells in HIV control, cure and prevention
  publication-title: Nat Rev Immunol
  doi: 10.1038/s41577-020-0274-9
– volume: 81
  start-page: 5225
  year: 2007
  ident: pone.0260118.ref012
  article-title: Enhanced detection of human immunodeficiency virus type 1 (HIV-1) Nef-specific T cells recognizing multiple variants in early HIV-1 infection
  publication-title: J Virol
  doi: 10.1128/JVI.02564-06
– volume: 274
  start-page: 221
  year: 2003
  ident: pone.0260118.ref013
  article-title: Optimized determination of T cell epitope responses
  publication-title: J Immunol Methods
  doi: 10.1016/S0022-1759(02)00423-4
– volume: 206
  start-page: 1273
  year: 2009
  ident: pone.0260118.ref025
  article-title: Genetic identity, biological phenotype and evolutionary pathways of transmitted/founder viruses in acute and early HIV-1 infection
  publication-title: J Exp Med
  doi: 10.1084/jem.20090378
– volume: 5
  start-page: e14330
  year: 2010
  ident: pone.0260118.ref028
  article-title: Equivalence of ELISpot assays demonstrated between major HIV network laboratories
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0014330
– volume: 55
  start-page: 65
  year: 2002
  ident: pone.0260118.ref034
  article-title: rtREV: an amino acid substitution matrix for inference of retrovirus and reverse transcriptase phylogeny
  publication-title: J. Molecular Evolution
  doi: 10.1007/s00239-001-2304-y
– volume: 9
  start-page: e90378
  year: 2014
  ident: pone.0260118.ref018
  article-title: Broad HIV epitope specificity and viral inhibition induced by multigenic HIV-1 adenovirus subtype 35 vector vaccine in healthy uninfected adults
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0090378
– ident: pone.0260118.ref038
  doi: 10.2172/1458915
– volume: 73
  start-page: 1071
  year: 2008
  ident: pone.0260118.ref014
  article-title: Optimizing peptide matrices for identifying T-cell antigens
  publication-title: Cytometry A
  doi: 10.1002/cyto.a.20646
– volume: 31
  start-page: 1278
  year: 2015
  ident: pone.0260118.ref022
  article-title: Optimized Replicating Renilla Luciferase Reporter HIV-1 Utilizing Novel Internal Ribosome Entry Site Elements for Native Nef Expression and Function
  publication-title: AIDS Res Hum Retroviruses
  doi: 10.1089/aid.2015.0074
– volume: 84
  start-page: 4998
  year: 2010
  ident: pone.0260118.ref040
  article-title: Phenotypic and functional profile of HIV-inhibitory CD8 T cells elicited by natural infection and heterologous prime/boost vaccination
  publication-title: J Virol
  doi: 10.1128/JVI.00138-10
– volume: 7
  start-page: e30963
  year: 2012
  ident: pone.0260118.ref035
  article-title: International technology transfer of a GCLP-compliant HIV-1 neutralizing antibody assay for human clinical trials
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0030963
– volume: 16
  start-page: 147
  year: 2009
  ident: pone.0260118.ref027
  article-title: Concordant proficiency in measurement of T-cell immunity in human immunodeficiency virus vaccine clinical trials by peripheral blood mononuclear cell and enzyme-linked immunospot assays in laboratories from three continents
  publication-title: Clin Vaccine Immunol
  doi: 10.1128/CVI.00326-08
– volume: 409
  start-page: 161
  year: 2014
  ident: pone.0260118.ref020
  article-title: Development of a luciferase based viral inhibition assay to evaluate vaccine induced CD8 T-cell responses
  publication-title: J Immunol Methods
  doi: 10.1016/j.jim.2013.11.021
– volume: 53
  start-page: 102682
  year: 2020
  ident: pone.0260118.ref039
  article-title: Understanding the mechanisms driving the spread of subtype C HIV-1
  publication-title: EBioMedicine
  doi: 10.1016/j.ebiom.2020.102682
– volume: 35
  start-page: 1547
  year: 2018
  ident: pone.0260118.ref032
  article-title: MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms
  publication-title: Molecular Biology and Evolution
  doi: 10.1093/molbev/msy096
– volume: 86
  start-page: 2715
  year: 2012
  ident: pone.0260118.ref021
  article-title: Generation of transmitted/founder HIV-1 infectious molecular clones and characterization of their replication capacity in CD4 T lymphocytes and monocyte-derived macrophages
  publication-title: J Virol
  doi: 10.1128/JVI.06157-11
– volume: 92
  start-page: e00484
  year: 2018
  ident: pone.0260118.ref023
  article-title: Incomplete downregulation of CD4 expression affects HIV-1 Env conformation and antibody-dependent cellular cytotoxicity responses
  publication-title: J Virol
  doi: 10.1128/JVI.00484-18
– volume: 299
  start-page: 1515
  year: 2003
  ident: pone.0260118.ref009
  article-title: Consensus and ancestral state HIV vaccines
  publication-title: Science
  doi: 10.1126/science.299.5612.1515c
– volume: 201
  start-page: 720
  year: 2010
  ident: pone.0260118.ref017
  article-title: Viral inhibition assay: a CD8 T cell neutralization assay for use in clinical trials of HIV-1 vaccine candidates
  publication-title: J Infect Dis
  doi: 10.1086/650492
– volume: 11
  start-page: e0147812
  year: 2016
  ident: pone.0260118.ref015
  article-title: Pooled-peptide epitope mapping strategies are efficient and highly sensitive: an evaluation of methods for identifying human T cell epitope specificities in large-scale HIV vaccine efficacy trials
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0147812
SSID ssj0053866
Score 2.4168699
Snippet Full characterisation of functional HIV-1-specific T-cell responses, including identification of recognised epitopes linked with functional antiviral...
SourceID plos
doaj
pubmedcentral
proquest
gale
pubmed
crossref
SourceType Open Website
Open Access Repository
Aggregation Database
Index Database
Enrichment Source
StartPage e0260118
SubjectTerms Adult
Amino acids
Analysis
Antibodies
Antigenic determinants
Antiretroviral drugs
Antiviral drugs
Biology and Life Sciences
CD3 antigen
CD4 antigen
CD8 antigen
CD8-Positive T-Lymphocytes - immunology
Diagnosis
Enzyme-linked immunosorbent assay
Epitopes
Epitopes, T-Lymphocyte - immunology
Female
gag Gene Products, Human Immunodeficiency Virus - immunology
Gag protein
Health aspects
HIV
HIV (Viruses)
HIV Infections - immunology
HIV Infections - virology
HIV-1 - immunology
Human immunodeficiency virus
Humans
Immunology
Infections
Laboratories
Lymphocytes T
Male
Medicine and Health Sciences
nef Gene Products, Human Immunodeficiency Virus - immunology
Peptides
Peptides - immunology
Peripheral blood mononuclear cells
Properties
Recognition
Replication
Research and Analysis Methods
Supervision
T cells
Vaccines
Virus Replication - drug effects
γ-Interferon
SummonAdditionalLinks – databaseName: DOAJ Open Access Full Text
  dbid: DOA
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9QwELbQnrggyquBggYJiVZqWu_GcZxjW7YsSFQ9bFFvkeMHG2lJot2Uv81fYCbJhl1UqRduSfyM_Xk8Tr6ZYewDt9Yby0WoY89Dkcs01Khlh95PcqMcYsSQ7fC3Kzm7EV9v49utUF_ECevcA3cDd6q80bFSsfA-FhQe2eRaTsjAU5B3ck_Sl6d8c5jqZDCuYil7Q7koGZ_283JSV6U7ab1oUZCPrY2o9dc_SOVRvazW96mc_zInt7aiy6fsSa9DwlnX9z32yJXP2F6_Stdw2LuSPnrOfp-jTmibBVQeLj4pmIf0oR5acxFUNaEoF0XekrYox8oNP7Pp1raUDQezL9_DMTS0qf0sGiwWeorF5FZQIHBJVwVDMT66S_qwC6hVQv636YGlhBVTelGCBiKzr9yiI9D3jXzWP47hyvljmJa_QJcWrqsl1FVDnCZ85b7_rkY5VDs4vJ5Pj6AmYo51sHbNC3ZzOZ1fzMI-xENo4jRqQqF9rFOZo9rE7djlsUolaZzCO6ONVS6KcxHZlDs89nhuYqlkmjoReWe5R2n1ko1KnNR9BkJ6jyd9ro2QQjusKE2sQ5T4JM69zQMWbeY7M73_cwrDsczan3oJnoO66csIJVmPkoCFQ6m68__xQP5zgtKQl7x3tw8Q01mP6ewhTAfsIwExIxmDXcSR6EwlsB3y1pWdSQpTIBPJA3awkxNlg9lJ3icob3q6znAZKjwByEmCJTfwvj_5_ZBMlRIVr3TVXZsn5ZIMmAP2qlsNw9tGFIwhEmnAkp11sjMcuyllsWidmitsmo_V6_8xfm_Y4wlRj4itmRywUbO6c29Rd2zyd62Y-APz63A_
  priority: 102
  providerName: Directory of Open Access Journals
– databaseName: ProQuest Central
  dbid: BENPR
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwhV1bb9MwFLZG98ILYtyWMZCRkNikZUsb20keEFpHR0GiqtCG9hY5vqyRShLajL_NX-CcxMlWNMFbU18TH38-tr9zDiFvA62t0gHzJbeBzzKR-BK0bN_aUaZiAzKi0Hb460xML9mXK361RWadLQzSKjtMbIBalwrPyE-gghh0FzGKPlQ_fYwahberXQgN6UIr6PeNi7EHZBsgmQcDsj2ezObfOmyG2S2EM6ALo-GJG6_jqizMceNdC4N_3FmgGj_-PVoPqmW5vk8V_ZtReWeJOn9MHjndkp62wrBDtkzxhOy42bumB87F9OFT8nsMuqKuF7S09OxjTC98PMCnjRkJqKA0LxZ51pC5MMfK9Jfc-KgbKoeh08_f_SGtcbH7kddQzLcYo8msaA4CjTosVRj7o_2JB74UtE2a3Tbds5egYkzPCyopktxXZtES610jn-T1EZ0Ze0QnxS8qC03n5ZJWZY1cJ3hl139TAT5Vhh7MLyaHtELCjjZ0bepn5PJ8cnE29V3oB1_xJKx9Ji2XichAnQr00GQ8TgRqoswaJZWOTcgzFuokMLAdsoHiIhZJYlhojQ4soNhzMihgUHcJZcLaBIBNKiaYNFBREmkD-1ob8czqzCNhN96pcn7RMTzHMm0u-yLYH7XDl6KUpE5KPOL3parWL8h_8o9RlPq86NW7-aNcXacOJNLYKsnjmDNrOcNQ4CqTYoTGzAw98VuPvENBTBF7oIvwJVoTCmgHvXilpwLDF4hIBB7Z38gJmKE2kndRlLuertPb2QUlO_G-P_lNn4yVIkWvMOVNkycJBBo2e-RFOxv6tw0xSEPIEo9EG_Nk43NsphT5onF2HkPTwTDe-3e3XpKHIyQbIT8z2ieDenVjXoG2WGevHQT8AWKkb2I
  priority: 102
  providerName: ProQuest
Title Breadth of CD8 T-cell mediated inhibition of replication of diverse HIV-1 transmitted-founder isolates correlates with the breadth of recognition within a comprehensive HIV-1 Gag, Nef, Env and Pol potential T-cell epitope (PTE) peptide set
URI https://www.ncbi.nlm.nih.gov/pubmed/34788349
https://www.proquest.com/docview/2598507627
https://www.proquest.com/docview/2599069647
https://pubmed.ncbi.nlm.nih.gov/PMC8598018
https://doaj.org/article/8fca58854ff544408cba62235840212f
http://dx.doi.org/10.1371/journal.pone.0260118
Volume 16
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3db9MwELe27oUXxPhax6iMhMQmLVPSOI7zgNBa2hUkqgq1qG9R4o-1UklCmyH4q_kXuHPSQFERL7xEbfwZ--58tn93R8hLVykjlcucJDCuw1IeOQlo2Y4x3VQKDTQi0Xb4w5iPZuz9PJgfkG3M1noAN3u3dhhParZeXX378v0NMPxrG7Uh9LaFroo801fWR5YnDsmRvTFCMB9r7hWAuzmvDej-VnJngbJ-_Btp3SpW-WafKvonovK3JWr4gNyvdUt6XRHDMTnQ2UNyXHPvhp7XLqYvHpEfPdAVVbmguaH9t4JOHTzAp9aMBFRQuswWy9SCuTDHWjeX3PhXWSiHpqN3nxyPlrjYfV6WUMwxGKNJr-kSCBp1WCox9kf1Ew98KWibNP3VdINegooxfZnRhCLIfa0XFbC-buQmub2kY20u6SD7SpNM0Um-okVeItYJPrnuvy5APhWank-mgwtaIGBHabrR5WMyGw6m_ZFTh35wZBD5pcMSEyQRT0GdcpWn00BEHDVRZrRMpBLaD1Lmq8jVsB0yrgy44FGkmW-0cg1IsSeklcGknhDKuDERCLZEMs4SDRVFodKwrzVhkBqVtom_ne9Y1n7RMTzHKraXfSHsj6rpi5FK4ppK2sRpShWVX5B_5O8hKTV50au3fZGvb-NaSMTCyCQQImDGBAxDgcs04V00Zmboid-0ySskxBi5AboII1GZUEA76MUrvuYYvoCH3G2Ts52cIDPkTvIJkvK2p5sY2FPAzoB3Qyi5Je_9yS-aZKwUIXqZzu9snsjlaNjcJk8rbmi-1scgDT6L2iTc4ZOd4dhNyZYL6-xcQNOuJ07_x_g9I_e6CElCFGd4Rlrl-k4_B52yTDvkMJyH8BR9D5_Dmw456g3Gk48de0rTsWLkJ3XJgDQ
linkProvider Scholars Portal
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1bb9MwFLZG9wAviHFbYYCRQGzSsqWN4yQPE9qlo2VbVaEO7S04vqyVRhLaDMSf4y_xFzgncbIVTfC0t6a-Jj4-Pra_cz5C3rhKGalc5gjfuA5LeOQIsLIdY7qJDDXIiETf4ZMh75-yj2f-2RL5VfvCIKyy1omlolaZxDPybaggBNuFd4P3-TcHWaPwdrWm0BCWWkHtlCHGrGPHkf75A7Zw853BAYz32273sDfe7zuWZcCRfuQVDhPGFxFPYOV2VUcnfhhxNHqY0VJIFWrPT5inIleD5W1c6fOQR5FmntHKNTBhoN47ZJnhAUqLLO_1hqNP9VoA2oRz67DnBZ1tKx9beZbqrTKaF5KNXFsQS96AZnVo5RfZ_CbT928E57Ul8fABuW9tWbpbCd8KWdLpQ7JitcWcrtuQ1huPyO89sE1VMaGZofsHIR07eGFAS7cVMHnpNJ1MkxI8hjlmurlUx0dVQkc07Q8-Ox1a4OL6dVpAMccgJ5Se0SlMILSZqUSukeonHjBTsG5pctV0g5aCijF9mlJBEVQ_05MKyG8b-SDON-lQm03aS79TkSo6yi5onhWIrYJXtv3XOejDXNP10bi3QXMECClN57p4TE5vRQiekFYKg7pKKOPGRKBIhWScCQ0VRYHSsI82gZ8YlbSJV493LG0cdqQDuYjLy8UA9mPV8MUoJbGVkjZxmlJ5FYfkP_n3UJSavBhFvPwjm53HVinFoZHCD0OfGeMzpB6XieBddJ5mGPnftMk7FMQYdR10Eb5E5bIB7WDUsHiXI10CD7jbJmsLOUFHyYXkVRTluqfz-Go2Q8lavG9Oft0kY6UICUx1dlnmiVyOjtRt8rSaDc3bekgK4bGoTYKFebLwORZT0umkDK4eQtNuJ3z27269Inf745Pj-HgwPHpO7nUR6ITY0GCNtIrZpX4BlmqRvLTqgJIvt62B_gAOKaxJ
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1fb9MwELdGJyFeEOPfCgOMBGKTli1tHCd5mNC6tWwMqgp1aG_B8Z-1UklCm4H4inwZvgJ3iZOtaIKnvTW1Yzvx-e4c_-5-hLxylTJSucwRvnEdlvDIEeBlO8Z0ExlqkBGJscMfh_zolL0_889WyK86FgZhlbVOLBW1yiR-I9-FBkLwXThs1Y2FRYwOB2_zbw4ySOFJa02nISzNgtor043ZII8T_fMHbOcWe8eHMPevu91Bf3xw5FjGAUf6kVc4TBhfRDwBK-6qjk78MOLoADGjpZAq1J6fME9FrgYv3LjS5yGPIs08o5VrYPFAu7fIagBWHzaCq73-cPSptgugWTi3wXte0Nm1srKTZ6neKTN7IfHIFeNYcgg0lqKVz7LFdW7w32jOK-ZxcI_ctX4t3a8EcY2s6PQ-WbOaY0E3bXrrrQfkdw_8VFVMaGbowWFIxw4eHtAyhAXcXzpNJ9OkBJJhjbluDtjxUpUwEk2Pjj87HVqgof06LeA2xyA_lJ7TKSwm9J-pRN6R6id-bKbg6dLksusGOQUNY_k0pYIiwH6uJxWo33byTpxv06E227SffqciVXSUzWieFYizgke249c56MZc083RuL9FcwQLKU0XunhITm9ECB6RVgqTuk4o48ZEoFSFZJwJDQ1FgdKwpzaBnxiVtIlXz3csbU52pAaZxeVBYwB7s2r6YpSS2EpJmzjNXXmVk-Q_9XsoSk1dzChe_pHNz2OroOLQSOGHoc-M8RnSkMtE8C4GUjNkATBt8gYFMUa9B0OEN1GFb0A_mEEs3udIncAD7rbJxlJN0FdyqXgdRbke6SK-XNlwZy3e1xe_bIqxUYQHpjq7KOtELseg6jZ5XK2G5mk9JIjwWNQmwdI6WXodyyXpdFImWg-ha7cTPvn3sF6Q26CJ4g_Hw5On5E4XMU8IEw02SKuYX-hn4LQWyXOrDSj5ctMK6A89d7CN
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Breadth+of+CD8+T-cell+mediated+inhibition+of+replication+of+diverse+HIV-1+transmitted-founder+isolates+correlates+with+the+breadth+of+recognition+within+a+comprehensive+HIV-1+Gag%2C+Nef%2C+Env+and+Pol+potential+T-cell+epitope+%28PTE%29+peptide+set&rft.jtitle=PloS+one&rft.au=Peter+Hayes&rft.au=Natalia+Fernandez&rft.au=Christina+Ochsenbauer&rft.au=Jama+Dalel&rft.date=2021-11-17&rft.pub=Public+Library+of+Science+%28PLoS%29&rft.eissn=1932-6203&rft.volume=16&rft.issue=11&rft.spage=e0260118&rft_id=info:doi/10.1371%2Fjournal.pone.0260118&rft.externalDBID=DOA&rft.externalDocID=oai_doaj_org_article_8fca58854ff544408cba62235840212f
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1932-6203&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1932-6203&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1932-6203&client=summon