芒果采后病害拮抗菌的筛选、鉴定及其生防效果研究

采用平板对峙法从芒果叶片与果实分离筛选芒果采后主要病害病原菌（胶孢炭疽病菌Colletotrichum gloeosporioides Penz.,芒果蒂腐病菌Botryodiplodia theobromae Pat.）的拮抗菌,对抑菌效果好的拮抗菌进行采后病害防治试验,并采用形态及培养特征观察、生理生化测定结合16S DNA序列分析进行鉴定。发现菌株A1稀释10倍的LB菌液对芒果采后炭疽病防效为80.05%、对蒂腐病的防效为74.18%,平均防效达到77.12%,防效明显。菌株C13对芒果采后炭疽病和蒂腐病防效次之,防效分别为71.73%和54.24%,平均防效达到62.99%。经鉴定A1...

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Bibliographic Details
Published in西南农业学报 Vol. 29; no. 2; pp. 385 - 389
Main Author 赵超 胡美姣 李敏 高兆银
Format Journal Article
LanguageChinese
Published 海南大学环境与植物保护学院,海南海口,570228%中国热带农业科学院环境与植物保护研究所,海南海口,571101 2016
Subjects
拮抗菌
芒果
采后病害
Antsgonists
Mango
Postharvest
diseases
芒果
采后病害
拮抗菌
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ISSN1001-4829
DOI10.16213/j.cnki.acjas.2016.02.032

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Summary:采用平板对峙法从芒果叶片与果实分离筛选芒果采后主要病害病原菌（胶孢炭疽病菌Colletotrichum gloeosporioides Penz.,芒果蒂腐病菌Botryodiplodia theobromae Pat.）的拮抗菌,对抑菌效果好的拮抗菌进行采后病害防治试验,并采用形态及培养特征观察、生理生化测定结合16S DNA序列分析进行鉴定。发现菌株A1稀释10倍的LB菌液对芒果采后炭疽病防效为80.05%、对蒂腐病的防效为74.18%,平均防效达到77.12%,防效明显。菌株C13对芒果采后炭疽病和蒂腐病防效次之,防效分别为71.73%和54.24%,平均防效达到62.99%。经鉴定A1为荧光假单胞菌Pseudomonas fluorescens Migula,C13为枯草芽孢杆菌Bacillus subtilis strain。
Bibliography:Mango; Poatharvest diseases; Antagonists
51-1213/S
Leaves and fruits of mango plant were chosen as initial inoculums to isolate antagonists on the main pathogenic fungi Colletotrichum gloeosporioides Penz. and Botryodiplodia theobromae Pat. of mange fruit. Biocontrol effects were evaluated by the method of confronting cul- tures. The high antagonistic activity stains were selected to control postharvest diseases and to determine its classification status by morpholo- gy and culture feature observation, and physiological and biochemical characteristics determination and 16S rDNA sequences assay. The re- sults showed that strain A1 had a high control effect,followed by strain C13. The disease control effect of 10 times dilution oral LB medium on anthracnose and on stem-end rot was 80.05 % and 74.14 % respectively, and average inhibitory rate reached 77.12 % against the 2 pathogens. The disease control effect of strain C13 on anthracnose and on stem-end rot was 71.73 % and 54.24 % respectively, and average inhibit
ISSN:1001-4829
DOI:10.16213/j.cnki.acjas.2016.02.032