甘蔗品种RSD感病性检测及RSD病原菌分离培养

为了解不同甘蔗品种的甘蔗宿根矮化病（RSD）感病情况,采用PCR检测方法,对13个甘蔗品种进行RSD感病率检测.结果显示,在所检测的13个甘蔗品种中,不同品种感病率有所差异,其中所检测的2个CP系列感病率最低.同时,利用MSC培养基从感染RSD的甘蔗蔗汁中分离培养到一种生长缓慢、菌落颜色为乳白色的杆状细菌.根据菌落形态、显微镜观察,以及特异引物和16S rDNA扩增多种方法鉴定,最终确认所分离到的杆状细菌即为甘蔗宿根矮化病病原菌（Lei sonia xyli subsp.xyli）....

Full description

Saved in:
Bibliographic Details
Published in广东农业科学 Vol. 42; no. 9; pp. 62 - 67
Main Author 王丽 张剑亮 王继华 曹干
Format Journal Article
LanguageChinese
Published 广东省农科院作物研究所/广东省农作物遗传改良重点实验室,广东广州,510640 2015
Subjects
分离培养
感病率
甘蔗宿根矮化病（RSD）
感病率
分离培养
甘蔗宿根矮化病(RSD)
Online AccessGet full text
ISSN1004-874X

Cover

Abstract 为了解不同甘蔗品种的甘蔗宿根矮化病（RSD）感病情况,采用PCR检测方法,对13个甘蔗品种进行RSD感病率检测.结果显示,在所检测的13个甘蔗品种中,不同品种感病率有所差异,其中所检测的2个CP系列感病率最低.同时,利用MSC培养基从感染RSD的甘蔗蔗汁中分离培养到一种生长缓慢、菌落颜色为乳白色的杆状细菌.根据菌落形态、显微镜观察,以及特异引物和16S rDNA扩增多种方法鉴定,最终确认所分离到的杆状细菌即为甘蔗宿根矮化病病原菌（Lei sonia xyli subsp.xyli）.
AbstractList 为了解不同甘蔗品种的甘蔗宿根矮化病（RSD）感病情况,采用PCR检测方法,对13个甘蔗品种进行RSD感病率检测.结果显示,在所检测的13个甘蔗品种中,不同品种感病率有所差异,其中所检测的2个CP系列感病率最低.同时,利用MSC培养基从感染RSD的甘蔗蔗汁中分离培养到一种生长缓慢、菌落颜色为乳白色的杆状细菌.根据菌落形态、显微镜观察,以及特异引物和16S rDNA扩增多种方法鉴定,最终确认所分离到的杆状细菌即为甘蔗宿根矮化病病原菌（Lei sonia xyli subsp.xyli）.
S432.4+4; 为了解不同甘蔗品种的甘蔗宿根矮化病(RSD)感病情况,采用PCR检测方法,对13个甘蔗品种进行RSD感病率检测.结果显示,在所检测的13个甘蔗品种中,不同品种感病率有所差异,其中所检测的2个CP系列感病率最低.同时,利用MSC培养基从感染RSD的甘蔗蔗汁中分离培养到一种生长缓慢、菌落颜色为乳白色的杆状细菌.根据菌落形态、显微镜观察,以及特异引物和16S rDNA扩增多种方法鉴定,最终确认所分离到的杆状细菌即为甘蔗宿根矮化病病原菌(Lei sonia xyli subsp.xyli).
Author 王丽 张剑亮 王继华 曹干
AuthorAffiliation 广东省农科院作物研究所/广东省农作物遗传改良重点实验室,广东广州510640
AuthorAffiliation_xml – name: 广东省农科院作物研究所/广东省农作物遗传改良重点实验室,广东广州,510640
Author_FL CAO Gan
WANG Ji-hua
ZHANG Jian-liang
WANG Li
Author_FL_xml – sequence: 1
  fullname: WANG Li
– sequence: 2
  fullname: ZHANG Jian-liang
– sequence: 3
  fullname: WANG Ji-hua
– sequence: 4
  fullname: CAO Gan
Author_xml – sequence: 1
  fullname: 王丽 张剑亮 王继华 曹干
BookMark eNrjYmDJy89LZWHgNDQwMNG1MDeJ4GDgLS7OTDIwMDQ0MDQyMORksHw-ZcaLKdOfTm58vrw3KNjlWcv859NbnzUsf7a44dnW7qf9XUBBoMjTvvkv-nuedrQ9X7b76fydT1t38zCwpiXmFKfyQmluhhA31xBnD10ff3dPZ0cf3WRTSyNdcwMTiyRTwyQLo9Rkk2RDw-TE1GQjwyQgsjQxMEgySktONTJMTk4ysEg1TjZPMU41szAyNTdPskg1MLdMMjI15mZQhxhbnpiXlpiXHp-VX1qUB7QwPj0lrzK7AugLUwNLoG-AKpUgKpMz8vPSCzOBaguKMnMTiyrjzcxMDUwtTSwsjAHJsWCO
ClassificationCodes S432.4+4
ContentType Journal Article
Copyright Copyright © Wanfang Data Co. Ltd. All Rights Reserved.
Copyright_xml – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved.
DBID 2RA
92L
CQIGP
W95
~WA
2B.
4A8
92I
93N
PSX
TCJ
DatabaseName 维普_期刊
中文科技期刊数据库-CALIS站点
中文科技期刊数据库-7.0平台
中文科技期刊数据库-农业科学
中文科技期刊数据库- 镜像站点
Wanfang Data Journals - Hong Kong
WANFANG Data Centre
Wanfang Data Journals
万方数据期刊 - 香港版
China Online Journals (COJ)
China Online Journals (COJ)
DatabaseTitleList

DeliveryMethod fulltext_linktorsrc
DocumentTitleAlternate Identification of ratoon stunting disease （RSD） of different sugarcane varieties and isolation of RSD pathogen
DocumentTitle_FL Identification of ratoon stunting disease (RSD) of different sugarcane varieties and isolation of RSD pathogen
EndPage 67
ExternalDocumentID gdnykx201509012
665059488
GrantInformation_xml – fundername: 广州市科技计划项目; 广东省农业科学院院长基金
  funderid: (2013J4100066); (201208)
GroupedDBID -04
2B.
2B~
2RA
5XA
5XE
92G
92I
92L
ABDBF
ACGFS
ALMA_UNASSIGNED_HOLDINGS
CCEZO
CHDYS
CQIGP
CW9
GROUPED_DOAJ
TCJ
TGD
U1G
U5N
W95
~WA
4A8
93N
ABJNI
PSX
ID FETCH-LOGICAL-c592-7048b51b82ec4c11caec21b21b9400b2fce21ccb08e3c7d3e682577b8e079b253
ISSN 1004-874X
IngestDate Thu May 29 03:55:16 EDT 2025
Wed Feb 14 10:29:17 EST 2024
IsPeerReviewed true
IsScholarly true
Issue 9
Keywords 感病率
分离培养
甘蔗宿根矮化病(RSD)
Language Chinese
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-c592-7048b51b82ec4c11caec21b21b9400b2fce21ccb08e3c7d3e682577b8e079b253
Notes 44-1267/S
sugarcane ratoon stunting disease （RSD）; infective rate; isolation
In order to understand the infective rate of ratoon stunting disease （RSD）of different sugarcane varieties, 13 sugarcane varieties were identified by PCR. The results showed that the infective rates of different sugarcane varieties were different, and two CP sugarcane varieties had the lowest infective rates. And a kind of coryneform bacterium with slow growth and milky white colony was isolated from RSD infected sugarcane juice by MSC culture. By colonial morphology, microscopic examination, specific primers and identification of 16S rDNA, the coryneform bacterium was identified as Leifsonia xyli subsp, xyli, the pathogen of sugarcane RSD.
WANG Li, ZHANG Jian-liang, WANG Ji-hua, CAO Gan （ Crop Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Crops Genetics and Improvement, Guangzhou 510640, China）
PageCount 6
ParticipantIDs wanfang_journals_gdnykx201509012
chongqing_primary_665059488
PublicationCentury 2000
PublicationDate 2015
PublicationDateYYYYMMDD 2015-01-01
PublicationDate_xml – year: 2015
  text: 2015
PublicationDecade 2010
PublicationTitle 广东农业科学
PublicationTitleAlternate Guangdong Agricultural Sciences
PublicationTitle_FL Guangdong Agricultural Sciences
PublicationYear 2015
Publisher 广东省农科院作物研究所/广东省农作物遗传改良重点实验室,广东广州,510640
Publisher_xml – name: 广东省农科院作物研究所/广东省农作物遗传改良重点实验室,广东广州,510640
SSID ssib001101201
ssib017478013
ssib051371967
ssj0000993252
Score 2.006713
Snippet 为了解不同甘蔗品种的甘蔗宿根矮化病（RSD）感病情况,采用PCR检测方法,对13个甘蔗品种进行RSD感病率检测.结果显示,在所检测的13个甘蔗品种中,不同品种感病率有所差异,其中...
S432.4+4; 为了解不同甘蔗品种的甘蔗宿根矮化病(RSD)感病情况,采用PCR检测方法,对13个甘蔗品种进行RSD感病率检测.结果显示,在所检测的13个甘蔗品种中,不同品种感病率有所...
SourceID wanfang
chongqing
SourceType Aggregation Database
Publisher
StartPage 62
SubjectTerms 分离培养
感病率
甘蔗宿根矮化病（RSD）
Title 甘蔗品种RSD感病性检测及RSD病原菌分离培养
URI http://lib.cqvip.com/qk/93806X/201509/665059488.html
https://d.wanfangdata.com.cn/periodical/gdnykx201509012
Volume 42
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
journalDatabaseRights – providerCode: PRVAON
  databaseName: DOAJ Directory of Open Access Journals
  issn: 1004-874X
  databaseCode: DOA
  dateStart: 20090101
  customDbUrl:
  isFulltext: true
  dateEnd: 99991231
  titleUrlDefault: https://www.doaj.org/
  omitProxy: true
  ssIdentifier: ssj0000993252
  providerName: Directory of Open Access Journals
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LaxRBEG6WnLyIEsUYlT3Yp2VkXj3dfZzZmSUIetAVclu2e3s2IEx8bEBzipAE8ZWDgXjbg2B-gQfJ75nd_AyreibDICIqLEPxVXVNTddsbXVvdzUhd0Nl65wzh3GeO2GOm5WFxxwYPIfKVyyXGuc7HjyMNp6E9zfZZqdz2Fq1tDNT9_Tub_eV_I9XAQO_4i7Zf_BsoxQAoMG_cAUPw_WvfEwzTmVIpaCZsASnGaMygPQQWTGnIn30OKVZRAVwB1YeQGYRFwWAiAOkgUgYFQlqEAMqYtuwkQcwsxqE5fYtAnRkbwRtbUMQSKRlMUDaiS-CwEpAQ0gTsLbSEFmiQuLaZukhK05B7cXrgBy4P1pnZZO0ZxX2aexaPdK2Al5M46zXEudoWWz7RaQA9vBBZVKbiRb57YmPatNnHaVx9Yzg1dLOizAe-q3XVbZich3tq1_36uyPX0psR5CmYtka3DMOCZvbGpnbrBJroLlNGPTwyIHWqJR5AYc4xpupPUi6A59V_7PXhmLljq3tYvoc8hG7PazIx8W0lckMr5DL9RCkG1fv01XS2d1aJXJ5_OX8-KT8_GZ5-hFcv9ifL08OFnuni697i-_vy6N3AAJSfpqfH30o3x4uv52V8x_lwdk1Mhxkw_6GUx-r4WgmYTgFMVsxTwnf6FB7nh4b7XsKPhLiufJzbXxPa-UKE2g-CUwkIKxzJYzLpfJZcJ2sFNuFuUG6LDdR4ObcuDbtzpU2HBJIzsdR7uUTd42sN089elZVTxk1nb1GunU_jOrv1MvRdFK8fvoKve1Courf_KOCdXIJJasJsVtkZfZix9yGFHGm7lgH_gSlzEvu
linkProvider Directory of Open Access Journals
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E7%94%98%E8%94%97%E5%93%81%E7%A7%8DRSD%E6%84%9F%E7%97%85%E6%80%A7%E6%A3%80%E6%B5%8B%E5%8F%8ARSD%E7%97%85%E5%8E%9F%E8%8F%8C%E5%88%86%E7%A6%BB%E5%9F%B9%E5%85%BB&rft.jtitle=%E5%B9%BF%E4%B8%9C%E5%86%9C%E4%B8%9A%E7%A7%91%E5%AD%A6&rft.au=%E7%8E%8B%E4%B8%BD+%E5%BC%A0%E5%89%91%E4%BA%AE+%E7%8E%8B%E7%BB%A7%E5%8D%8E+%E6%9B%B9%E5%B9%B2&rft.date=2015&rft.issn=1004-874X&rft.volume=42&rft.issue=9&rft.spage=62&rft.epage=67&rft.externalDocID=665059488
thumbnail_s http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fimage.cqvip.com%2Fvip1000%2Fqk%2F93806X%2F93806X.jpg
http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Fgdnykx%2Fgdnykx.jpg