紫果西番莲炭疽病病原菌鉴定及生物学特性研究
【目的】对紫果西番莲叶片炭疽病病原X-03进行分离鉴定,并对其生物学特性和杀菌剂对X-03的抑制作用进行研究。【方法】采用形态观察与分子生物学相结合的方法。【结果】X-03为刺盘孢属（Colletotrichum sp.）真菌,它的最适生长温度为15~20℃,最适生长p H为p H 8~9,当温度高于50℃时X-03菌株的孢子不能萌发。X-03生长最适培养基为PDA,最适碳源和氮源分别为葡萄糖和Na NO3。供试的杀菌剂中以福美双对X-03的抑制效果最好。【结论】研究可为紫果西番莲炭疽病的防治提供理论依据。...
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| Published in | 西南农业学报 Vol. 30; no. 6; pp. 1358 - 1363 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
楚雄师范学院化学与生命科学系,云南楚雄675000
2017
云南省高校应用生物学重点实验室,云南楚雄675000%楚雄师范学院化学与生命科学系,云南楚雄,675000 省部共建云南生物资源保护与利用国家重点实验室,云南昆明650091 云南省高校应用生物学重点实验室,云南楚雄675000%省部共建云南生物资源保护与利用国家重点实验室,云南昆明,650091%楚雄师范学院化学与生命科学系,云南楚雄675000 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1001-4829 |
| DOI | 10.16213/j.cnki.scjas.2017.6.021 |
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| Abstract | 【目的】对紫果西番莲叶片炭疽病病原X-03进行分离鉴定,并对其生物学特性和杀菌剂对X-03的抑制作用进行研究。【方法】采用形态观察与分子生物学相结合的方法。【结果】X-03为刺盘孢属（Colletotrichum sp.）真菌,它的最适生长温度为15~20℃,最适生长p H为p H 8~9,当温度高于50℃时X-03菌株的孢子不能萌发。X-03生长最适培养基为PDA,最适碳源和氮源分别为葡萄糖和Na NO3。供试的杀菌剂中以福美双对X-03的抑制效果最好。【结论】研究可为紫果西番莲炭疽病的防治提供理论依据。 |
|---|---|
| AbstractList | S667.9; [目的]对紫果西番莲叶片炭疽病病原X-03进行分离鉴定,并对其生物学特性和杀菌剂对X-03的抑制作用进行研究.[方法]采用形态观察与分子生物学相结合的方法.[结果]X-03为刺盘孢属(Colletotrichum sp)真菌,它的最适生长温度为l5～20℃,最适生长pH为pH 8～9,当温度高于50℃时X-03菌株的孢子不能萌发.X-03生长最适培养基为PDA,最适碳源和氮源分别为葡萄糖和NaNO3.供试的杀菌剂中以福美双对X-03的抑制效果最好.[结论]研究可为紫果西番莲炭疽病的防治提供理论依据. 【目的】对紫果西番莲叶片炭疽病病原X-03进行分离鉴定,并对其生物学特性和杀菌剂对X-03的抑制作用进行研究。【方法】采用形态观察与分子生物学相结合的方法。【结果】X-03为刺盘孢属（Colletotrichum sp.）真菌,它的最适生长温度为15~20℃,最适生长p H为p H 8~9,当温度高于50℃时X-03菌株的孢子不能萌发。X-03生长最适培养基为PDA,最适碳源和氮源分别为葡萄糖和Na NO3。供试的杀菌剂中以福美双对X-03的抑制效果最好。【结论】研究可为紫果西番莲炭疽病的防治提供理论依据。 |
| Abstract_FL | [Objective] The pathogen (X-03) of plant anthracnosis was identified from Passiflora edulis,and it's biological characteristics and inhibition effect of four fungicides on X-03 were investigated.[Method] Morphologic observation combined with molecular biological techniques were employed in the paper.[Result] The results showed that X-03 belonged to genus Colletotrichum,and the optimal growth condition for this fungus was 15-20 ℃ and pH 8-9,while the spores could not germinate when the temperature over 50 ℃.The most suitable medium was PDA for X-03,and the optimal C and N sources were glucose and NaNO3,respectively.The inhibitory effect of Thiram on X03 was more obvious than other tested germicides.[Conclusion] This study could provide a theoretical basis for the prevention and control of anthracnose of Passiflora edulis. |
| Author | 谢美华 杨金奎 李雪玲 杨海艳 陈莉莉 吴帆 |
| AuthorAffiliation | 楚雄师范学院化学与生命科学系,云南楚雄675000 省部共建云南生物资源保护与利用国家重点实验室,云南昆明650091 云南省高校应用生物学重点实验室,云南楚雄675000 |
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| Author_FL | YANG Jin-kui XIE Mei-hua CHEN Li-li WU Fan YANG Hai-yan LI Xue-ling |
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| DOI | 10.16213/j.cnki.scjas.2017.6.021 |
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| DocumentTitleAlternate | Study on Identification and Biological Characteristics of Anthracnose Pathogen of Passiflora edulis |
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| Keywords | 炭疽病 Anthracnosis 生物学性状 病原 紫果西番莲 Passiflora edulis Biological characteristics Pathogen |
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| Notes | 51-1213/S Passiflora edulis ; Anthracnosis ; Pathogen ; Biological characteristics [ Objective ] The pathogen （ X-03 ） of plant a.nthracnosis was identified from Passiflora edulis, and it＇s biological characteristics andinhibition effect of four fungicides on X-03 were investigated. [ Method ] Morphologic observation combined with molecular biological tech-niques were employed in the paper. [ Result ] The results showed that X-03 belonged to genus Colletotrichum, and the optimal growth condi-tion for this fungus was 15 -20 ℃ and pH 8 -9, while the spores could not germinate when the temperature over 50 ℃. The most suitablemedium was PDA for X-03, and the optimal C and N sources were glucose and NaNO3 , respectively. The inhibitory effect of Thiram on X-03 was more obvious than other tested germicides. [ Conclusion ] This study could provide a theoretical basis for the prevention and control ofanthraenose of Pass（flora edulis. XIE Mei-hua1＇2＇ , YANG Jiu-kui2 , LI Xne-ling, YANG Hai-yan , CHEN Li-li , WU Fan （ 1. |
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| PublicationTitle | 西南农业学报 |
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| Publisher | 楚雄师范学院化学与生命科学系,云南楚雄675000 云南省高校应用生物学重点实验室,云南楚雄675000%楚雄师范学院化学与生命科学系,云南楚雄,675000 省部共建云南生物资源保护与利用国家重点实验室,云南昆明650091 云南省高校应用生物学重点实验室,云南楚雄675000%省部共建云南生物资源保护与利用国家重点实验室,云南昆明,650091%楚雄师范学院化学与生命科学系,云南楚雄675000 |
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| Snippet | 【目的】对紫果西番莲叶片炭疽病病原X-03进行分离鉴定,并对其生物学特性和杀菌剂对X-03的抑制作用进行研究。【方法】采用形态观察与分子生物学相结合的方法。【结果】X-03为刺盘孢... S667.9; [目的]对紫果西番莲叶片炭疽病病原X-03进行分离鉴定,并对其生物学特性和杀菌剂对X-03的抑制作用进行研究.[方法]采用形态观察与分子生物学相结合的方法.[结果]X-03... |
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| Title | 紫果西番莲炭疽病病原菌鉴定及生物学特性研究 |
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