紫果西番莲炭疽病病原菌鉴定及生物学特性研究

【目的】对紫果西番莲叶片炭疽病病原X-03进行分离鉴定,并对其生物学特性和杀菌剂对X-03的抑制作用进行研究。【方法】采用形态观察与分子生物学相结合的方法。【结果】X-03为刺盘孢属（Colletotrichum sp.）真菌,它的最适生长温度为15~20℃,最适生长p H为p H 8~9,当温度高于50℃时X-03菌株的孢子不能萌发。X-03生长最适培养基为PDA,最适碳源和氮源分别为葡萄糖和Na NO3。供试的杀菌剂中以福美双对X-03的抑制效果最好。【结论】研究可为紫果西番莲炭疽病的防治提供理论依据。...

Full description

Saved in:
Bibliographic Details
Published in西南农业学报 Vol. 30; no. 6; pp. 1358 - 1363
Main Author 谢美华 杨金奎 李雪玲 杨海艳 陈莉莉 吴帆
Format Journal Article
LanguageChinese
Published 楚雄师范学院化学与生命科学系,云南楚雄675000 2017
云南省高校应用生物学重点实验室,云南楚雄675000%楚雄师范学院化学与生命科学系,云南楚雄,675000
省部共建云南生物资源保护与利用国家重点实验室,云南昆明650091
云南省高校应用生物学重点实验室,云南楚雄675000%省部共建云南生物资源保护与利用国家重点实验室,云南昆明,650091%楚雄师范学院化学与生命科学系,云南楚雄675000
Subjects
炭疽病
生物学性状
病原
紫果西番莲
炭疽病
Anthracnosis
生物学性状
病原
紫果西番莲
Passiflora edulis
Biological characteristics
Pathogen
Online AccessGet full text
ISSN1001-4829
DOI10.16213/j.cnki.scjas.2017.6.021

Cover

More Information
Summary:【目的】对紫果西番莲叶片炭疽病病原X-03进行分离鉴定,并对其生物学特性和杀菌剂对X-03的抑制作用进行研究。【方法】采用形态观察与分子生物学相结合的方法。【结果】X-03为刺盘孢属（Colletotrichum sp.）真菌,它的最适生长温度为15~20℃,最适生长p H为p H 8~9,当温度高于50℃时X-03菌株的孢子不能萌发。X-03生长最适培养基为PDA,最适碳源和氮源分别为葡萄糖和Na NO3。供试的杀菌剂中以福美双对X-03的抑制效果最好。【结论】研究可为紫果西番莲炭疽病的防治提供理论依据。
Bibliography:51-1213/S
Passiflora edulis ; Anthracnosis ; Pathogen ; Biological characteristics
[ Objective ] The pathogen （ X-03 ） of plant a.nthracnosis was identified from Passiflora edulis, and it＇s biological characteristics andinhibition effect of four fungicides on X-03 were investigated. [ Method ] Morphologic observation combined with molecular biological tech-niques were employed in the paper. [ Result ] The results showed that X-03 belonged to genus Colletotrichum, and the optimal growth condi-tion for this fungus was 15 -20 ℃ and pH 8 -9, while the spores could not germinate when the temperature over 50 ℃. The most suitablemedium was PDA for X-03, and the optimal C and N sources were glucose and NaNO3 , respectively. The inhibitory effect of Thiram on X-03 was more obvious than other tested germicides. [ Conclusion ] This study could provide a theoretical basis for the prevention and control ofanthraenose of Pass（flora edulis.
XIE Mei-hua1＇2＇ , YANG Jiu-kui2 , LI Xne-ling, YANG Hai-yan , CHEN Li-li , WU Fan （ 1.
ISSN:1001-4829
DOI:10.16213/j.cnki.scjas.2017.6.021