基于SSR标记的广东省甘蔗黑穗病菌遗传多样性研究

利用SSR标记分析了30个来自广东省甘蔗主产区的甘蔗黑穗病菌菌株的遗传多样性。用19对带型较稳定且条带清晰的引物从供试菌株群体中检测到了39个多态性位点，平均每个引物得到2．05条多态性带。UPGMA聚类分析表明，在约0．74的相似系数水平，SSR标记把30个菌株划分为5个类群，类群1有22个菌株，包含了绝大部分来自粤西和粤北的菌株及全部的广州市郊菌株；在约0.8的相似系数水平，类群1可以进一步分为2个亚群体，亚群体1包含了90％的粤北菌株．亚群体2则包含了50％的粤西菌株。研究结果表明：该菌的遗传多样性与菌株的地理来源呈显著相关，而与品种间的相关性不大。...

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Published in广东农业科学 Vol. 39; no. 24; pp. 154 - 157
Main Author 陈健文 冯淑杰 钟玫 刘睿 沈万宽 陈仲华 杨湛端 徐苑娴 邓海华
Format Journal Article
LanguageChinese
Published 广州甘蔗糖业研究所/广东省甘蔗改良与生物炼制重点实验室,广东广州,510316%华南农业大学园艺学院,广东广州,510642 2012
Subjects
SSR标记
甘蔗黑粉菌
遗传多样性
遗传多样性
甘蔗黑粉菌
SSR标记
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ISSN1004-874X

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Summary:利用SSR标记分析了30个来自广东省甘蔗主产区的甘蔗黑穗病菌菌株的遗传多样性。用19对带型较稳定且条带清晰的引物从供试菌株群体中检测到了39个多态性位点，平均每个引物得到2．05条多态性带。UPGMA聚类分析表明，在约0．74的相似系数水平，SSR标记把30个菌株划分为5个类群，类群1有22个菌株，包含了绝大部分来自粤西和粤北的菌株及全部的广州市郊菌株；在约0.8的相似系数水平，类群1可以进一步分为2个亚群体，亚群体1包含了90％的粤北菌株．亚群体2则包含了50％的粤西菌株。研究结果表明：该菌的遗传多样性与菌株的地理来源呈显著相关，而与品种间的相关性不大。
Bibliography:44-1267/S
CHEN Jian-wen, FENG Shu-jie, ZHONG Mei, LIU Rui, SHEN Wan-kuan, CHEN Zhong-hua, YANG Zhan-duan, XU Yuan-xian, DENG Hai-hua （1.Guangzhou Sugarcane Industry Research Institute/Guangdong Key Lob of Sugarcane Improvement ＆ Biorefinery, Guangzhou 510316 China; 2.College of Horticultural Science, South China Agricultural University, Guangzhou 510642, China）
The genetie diversity of 30 sugarcane smut strains from the main sugarcane growing area of Guangdong province was studied by simple sequence repeats （SSR） markers. 16 pairs of SSR primers, showing stable amplified band pattern and clear bands, were selected to analysis these strains. A total of 39 polymorphic loci were obtained and 2.05 polymorphic loci per SSR primer were detected. Based on SSR marker data at the similarity coefficient level of about 0.74, these 30 strains were divided into 5 groups by UPGMA clustering analysis. The first group consisted of 22 strains and could be further divided into 2 subgroups at the similarity coefficient level of a
ISSN:1004-874X