脑源性神经营养因子增强间充质干细胞抑制滤泡辅助性T细胞的作用
目的探讨脑源性神经营养因子（BDNF）增强间充质干细胞（MSC）抑制滤泡辅助性T细胞（Tfh细胞）的作用及机制。方法ELISA法检测MSC培养上清中吲哚胺2，3-二加氧酶（IDO）、IL．10、TGF—B和IL-2l的含量；采集健康志愿者的外周血标本，采用人淋巴细胞分离液分离外周血中的淋巴细胞；采用Transwell小室进行MSC和淋巴细胞共培养，流式细胞术检测CD4＋CXCR5＋Tfh细胞及其亚群的比例。结果@BDNF组（BDNF刺激的MSC）培养上清IL-10、TGF-β、IDO浓度均高于对照组（加入等体积磷酸盐缓冲液）[IL-10：（42．1±64．4）ng／ml对（19．3±2．1）n...
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| Published in | 中华血液学杂志 Vol. 39; no. 1; pp. 37 - 40 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
陆军军医大学附属西南医院血液科,重庆,400038
2018
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0253-2727 |
| DOI | 10.3760/cma.j.issn.0253-2727.2018.01.008 |
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| Abstract | 目的探讨脑源性神经营养因子（BDNF）增强间充质干细胞（MSC）抑制滤泡辅助性T细胞（Tfh细胞）的作用及机制。方法ELISA法检测MSC培养上清中吲哚胺2，3-二加氧酶（IDO）、IL．10、TGF—B和IL-2l的含量；采集健康志愿者的外周血标本，采用人淋巴细胞分离液分离外周血中的淋巴细胞；采用Transwell小室进行MSC和淋巴细胞共培养，流式细胞术检测CD4＋CXCR5＋Tfh细胞及其亚群的比例。结果@BDNF组（BDNF刺激的MSC）培养上清IL-10、TGF-β、IDO浓度均高于对照组（加入等体积磷酸盐缓冲液）[IL-10：（42．1±64．4）ng／ml对（19．3±2．1）ng／ml，t=4．761，P=0．009；TGF—β：（13．9±1．7）ng／ml对（5．3±0．6）ng／ml，t=5．129，P=0．008；IDO：（441．3±56．9）ng／ml对（226．7±37．6）ng／ml，t=3．130，P=O．035]；@BDNF组（淋巴细胞与BDNF刺激的MSC共培养）与MSC组（淋巴细胞与MSC共培养）比较：CD4＋CXCR5＋Tfh细胞比例降低[（3．37＋0．21）％对（6．51＋0．27）％，t=9．353，P〈0．001]，CD4＋CXCR5＋CXCR3＋CCR6-Tfhl细胞比例升高[（41．14±2．04）％对（26．72±2．57）％，t=4．383，P=0．012]，CD4＋CXCR5＋CXCR3CCR6-Tfh2细胞和CD4＋CXCR5＋CXCR3-CCR6＋Tfhl7细胞比例降低[Tfh2：（30．16：L5．38）％对（43．26＋4．11）％，t：4．426，P=0．012；Tfhl7：（15．61±1．52）％对（22．32±0．72）％，t=4．202，P：0．014]，CD4＋CXCR5＋Foxp3＋Tfr细胞比例升高[（4．95±0．22）％对（2．32±0．16）％，t=10．241，P〈0．001]，淋巴细胞培养上清中IL-21浓度降低[（0．28±0．03）ng／ml对（0．85±0．08）ng／ml，t=6．675，P=0．003]。结论BDNF能够增强MSC抑制Tfh细胞的作用，机制是抑制淋巴细胞中Tfh细胞比例升高及其向Tfh2和Tfhl7亚群的分化。 |
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| AbstractList | 目的探讨脑源性神经营养因子（BDNF）增强间充质干细胞（MSC）抑制滤泡辅助性T细胞（Tfh细胞）的作用及机制。方法ELISA法检测MSC培养上清中吲哚胺2，3-二加氧酶（IDO）、IL．10、TGF—B和IL-2l的含量；采集健康志愿者的外周血标本，采用人淋巴细胞分离液分离外周血中的淋巴细胞；采用Transwell小室进行MSC和淋巴细胞共培养，流式细胞术检测CD4＋CXCR5＋Tfh细胞及其亚群的比例。结果@BDNF组（BDNF刺激的MSC）培养上清IL-10、TGF-β、IDO浓度均高于对照组（加入等体积磷酸盐缓冲液）[IL-10：（42．1±64．4）ng／ml对（19．3±2．1）ng／ml，t=4．761，P=0．009；TGF—β：（13．9±1．7）ng／ml对（5．3±0．6）ng／ml，t=5．129，P=0．008；IDO：（441．3±56．9）ng／ml对（226．7±37．6）ng／ml，t=3．130，P=O．035]；@BDNF组（淋巴细胞与BDNF刺激的MSC共培养）与MSC组（淋巴细胞与MSC共培养）比较：CD4＋CXCR5＋Tfh细胞比例降低[（3．37＋0．21）％对（6．51＋0．27）％，t=9．353，P〈0．001]，CD4＋CXCR5＋CXCR3＋CCR6-Tfhl细胞比例升高[（41．14±2．04）％对（26．72±2．57）％，t=4．383，P=0．012]，CD4＋CXCR5＋CXCR3CCR6-Tfh2细胞和CD4＋CXCR5＋CXCR3-CCR6＋Tfhl7细胞比例降低[Tfh2：（30．16：L5．38）％对（43．26＋4．11）％，t：4．426，P=0．012；Tfhl7：（15．61±1．52）％对（22．32±0．72）％，t=4．202，P：0．014]，CD4＋CXCR5＋Foxp3＋Tfr细胞比例升高[（4．95±0．22）％对（2．32±0．16）％，t=10．241，P〈0．001]，淋巴细胞培养上清中IL-21浓度降低[（0．28±0．03）ng／ml对（0．85±0．08）ng／ml，t=6．675，P=0．003]。结论BDNF能够增强MSC抑制Tfh细胞的作用，机制是抑制淋巴细胞中Tfh细胞比例升高及其向Tfh2和Tfhl7亚群的分化。 目的 探讨脑源性神经营养因子(BDNF)增强间充质干细胞(MSC)抑制滤泡辅助性T细胞(Tfh细胞)的作用及机制.方法 ELISA法检测MSC培养上清中吲哚胺2,3-二加氧酶(IDO)、IL-10、TGF-β和IL-21的含量;采集健康志愿者的外周血标本,采用人淋巴细胞分离液分离外周血中的淋巴细胞;采用Transwell小室进行MSC和淋巴细胞共培养,流式细胞术检测CD4+CXCR5+Tfh细胞及其亚群的比例.结果 ①BDNF组(BDNF刺激的MSC)培养上清IL-10、TGF-β、IDO浓度均高于对照组(加入等体积磷酸盐缓冲液)[IL-10:(42.1 ±4.4) ng/ml对(19.3±2.1)ng/ml,t=4.761,P=0.009;TGF-β:(13.9±1.7)ng/ml对(5.3±0.6)ng/ml,t=5.129,P=0.008;IDO:(441.3±56.9)ng/ml对(226.7±37.6)ng/ml,t=3.130,P=0.035];②BDNF组(淋巴细胞与BDNF刺激的MSC共培养)与MSC组(淋巴细胞与MSC共培养)比较:CD4+CXCR5+Tfh细胞比例降低[(3.37±0.21)％对(6.51±0.27)％,t=9.353,P＜0.001],CD4+CXCR5+CXCR3+CCR6-Tfh1细胞比例升高[(41.14±2.04)％对(26.72±2.57)％,t=4.383,P=0.012],CD4+CXCR5+CXCR3-CCR6-Tfh2细胞和CD4+ CXCR5+ CXCR3-CCR6+ Tfh 17细胞比例降低[Tfh2:(30.16±5.38)％对(43.26±4.11)％,t=4.426,P=0.012;Tfh17:(15.61±1.52)％对(22.32±0.72)％,t=4.202,P=0.014],CD4+CXCR5+Foxp3+Tfr细胞比例升高[(4.95±0.22)％对(2.32±0.16)％,t=10.241,P＜0.001],淋巴细胞培养上清中IL-21浓度降低[(0.28±0.03)ng/ml对(0.85±0.08)ng/ml,t=6.675,P=0.003].结论 BDNF能够增强MSC抑制Tfh细胞的作用,机制是抑制淋巴细胞中Tfh细胞比例升高及其向Tfh2和Tfh17亚群的分化. |
| Abstract_FL | Objective To investigate the effect of brain derived neurotrophic factor (BDNF) on mesenchymal stem cells (MSC) inhibiting follicular helper T cells (Tfh cells).Methods The contents of indoleamine 2,3-dioxygenase (IDO),IL-10,TGF-β and IL-21 in MSC culture supernatant were detected by ELISA;The peripheral blood of healthy volunteers were collected,and lymphocyte in peripheral blood was separated by human lymphocyte separation solution;Co-cultures of MSC and lymphocyte were performed by Transwell chamber,and the proportion of CD4+CXCR5+Tfh cells and their subtypes were detected by flow cytometry.Results ①The concentrations of IL-10,TGF-β,and IDO in the supernatant of BDNF group (BDNF-stimulated MSC) were higher than those of the control ones (adding PBS with the same volume) [IL-10:(42.1±4.4) ng/ml vs (19.3±2.1) ng/ml,t=4.761,P=0.009;TGF-β:(13.9± 1.7) ng/ml vs (5.3 ± 0.6) ng/ml,t =5.129,P =0.008;IDO:(441.3 ± 56.9) ng/ml vs (226.7 ± 37.6) ng/ml,t =3.130,P =0.035];②The comparisons between BDNF (co-culture of lymphocyte and BDNF-stimulated MSC) and MSC groups (co-culture of lymphocyte and MSC) were detailed as of follows:the proportion of CD4 +CXCR5+Tfh cells were lower [(3.37±0.21)％ vs (6.51±0.27)％,t=9.353,P＜ 0.001],the proportion ofCD4+CXCR5+CXCR3+CCR6-Tfh cells were higher [(41.14±2.04)％ vs (26.72±2.57)％,t =4.383,P=0.012],CD4+CXCR5+CXCR3-CCR6-Tfh2 cells and CD4+CXCR5+CXCR3-CCR6+ Tfhl7 cells were lower [Tfh2:(30.16±5.38)％ vs (43.26±4.11)％,t=4.426,P=0.012;Tfh17:(15.61±1.52)％ vs (22.32±0.72)％,t =4.202,P =0.014],the proportion of CD4+CXCR5+Foxp3 + Tfr cells were higher [(4.95±0.22)％ vs (2.32±0.16)％,t =10.241,P ＜ 0.001],the concentration of IL-21 in the lymphocyte supernatant was lower [(0.28 ±0.03)ng/ml vs (0.85±0.08) ng/ml,t =6.675,P =0.003].Conclusion BDNF could enhance the inhibitory effect of MSC on Tfh cells through inhibiting the increasing of Tfh cells and the differentiations of Tfh2 and Tfh17 cells. |
| Author | 杨赛男;蒲欣;向莎丽;陈洁平;裴莉 |
| AuthorAffiliation | 陆军军医大学附属西南医院血液科,重庆400038 |
| AuthorAffiliation_xml | – name: 陆军军医大学附属西南医院血液科,重庆,400038 |
| Author_FL | Xiang Shali Pei Li Chen Jieping Pu Xin Yang Sainan |
| Author_FL_xml | – sequence: 1 fullname: Yang Sainan – sequence: 2 fullname: Pu Xin – sequence: 3 fullname: Xiang Shali – sequence: 4 fullname: Chen Jieping – sequence: 5 fullname: Pei Li |
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| Keywords | Mesenchymal stem cell 脑源性神经营养因子 T-lymphocyte subset T淋巴细胞亚群 Brain derived neurotrophic factor 间充质干细胞 |
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| Notes | Objective To investigate the effect of brain derived neurotrophic factor （BDNF） on mesenchymal stem cells （MSC） inhibiting follicular helper T cells （Tfh cells）. Methods The contents of indoleamine 2,3-dioxygenase （IDO）, IL-10, TGF-β and IL-21 in MSC culture supematant were detected by ELISA; The peripheral blood of healthy volunteers were collected, and lymphocyte in peripheral blood was separated by human lymphocyte separation solution; Co-cultures of MSC and lymphocyte were performed by Transwell chamber, and the proportion of CD4＋CXCR5 ＋ Tfh cells and their subtypes were detected by flow cytometry. Results （1）The concentrations of IL- 10, TGF-β, and IDO in the supernatant of BDNF group （BDNF-stimulated MSC） were higher than those of the control ones （adding PBS with the same volume） [IL-10： （42.1±4.4） ng/ml vs （19.3±2.1） ng/ml, t=4.761, P= 0.009; TGF-β： （13.9±1.7） ng/ml vs （5.3±0.6） ng/ml, t= 5.129, P= 0.008; IDO： （441.3±56.9） ng/ml vs （226.7±37.6） ng/ml, t= 3.130, P= 0.035]; （2）The comparisons between BD |
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| Snippet | 目的探讨脑源性神经营养因子（BDNF）增强间充质干细胞（MSC）抑制滤泡辅助性T细胞（Tfh细胞）的作用及机制。方法ELISA法检测MSC培养上清中吲哚胺2，3-二加氧... 目的 探讨脑源性神经营养因子(BDNF)增强间充质干细胞(MSC)抑制滤泡辅助性T细胞(Tfh细胞)的作用及机制.方法 ELISA法检测MSC培养上清中吲哚胺2,3-二加氧酶(IDO)、IL-10、TGF-β... |
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| SubjectTerms | T淋巴细胞亚群 脑源性神经营养因子 间充质干细胞 |
| Title | 脑源性神经营养因子增强间充质干细胞抑制滤泡辅助性T细胞的作用 |
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