广西眼镜蛇遗传多样性RAPD分析

对广西眼镜蛇的血液基因组进行RAPD扩增，从30条随机引物中筛选出18条可扩增出清晰多态条带的引物。结果表明，18条随机引物共产生了257个位点，其中多态性位点152个，多态率为59．1％；个体间的平均遗传相似系数为0．8186：个体间平均遗传距离为0．1814，群体Shannon信息指数为O．3170；Nei’s基因多样性指数为0．2112；等位基因观察数为1．3563；有效等俯基因数为1．5914。表明广西眼镜蛇具有较强的遗传多样性。...

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Bibliographic Details
Published in广东农业科学 Vol. 38; no. 19; pp. 128 - 129
Main Author 杨秀荣 邓继贤 蒋和生
Format Journal Article
LanguageChinese
Published 广西大学动物科学技术学院,广西南宁,530005%广西科学院/中国东盟(广西)生物技术合作研发中心,广西南宁,530002%广西大学动物科学技术学院,广西南宁530005 2011
广西科学院/中国东盟(广西)生物技术合作研发中心,广西南宁530002
Subjects
RAPD分析
广西眼镜蛇
遗传多样性
遗传多样性
RAPD分析
广西眼镜蛇
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ISSN1004-874X
DOI10.3969/j.issn.1004-874X.2011.19.047

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Summary:对广西眼镜蛇的血液基因组进行RAPD扩增，从30条随机引物中筛选出18条可扩增出清晰多态条带的引物。结果表明，18条随机引物共产生了257个位点，其中多态性位点152个，多态率为59．1％；个体间的平均遗传相似系数为0．8186：个体间平均遗传距离为0．1814，群体Shannon信息指数为O．3170；Nei’s基因多样性指数为0．2112；等位基因观察数为1．3563；有效等俯基因数为1．5914。表明广西眼镜蛇具有较强的遗传多样性。
Bibliography:44-1267/S
YANG Xiu-rong, DENG Ji-xian, JIANG He-sheng （1.College of Animal Science and Technology, Guangxi University, Nanning 530005, China; 2.Guangxi Aeademy of Seiences/China-A SEA N（ Guangxi ） Bioteehnology Cooperative Center, Nanning 530002, China）
PCR amplification was conducted for genome DNA pool of Guangxi Naja naja atra using 30 random primers. 18 random primers were selected to amplify genome DNA of Guangxi Naja naja atra. The results showed that 257 loci were detected, 152 of which （59.14%） were polymorphie. The genetic similarity index and average genetic distance were 0.8186 and 0.1814, respectively.The Shannon index and Nei＇s gene diversity were 0.3170 and 0.2112, respectively. The alleles observed number and effective numbers of alleles were 1.3563 and 1.5914, respectively. The research results showed that there was relatively higher genetic diversity in Guangxi Naja naja atra.
Guangxi Naja naja atra; genetic diversity; RAPD analysis
ISSN:1004-874X
DOI:10.3969/j.issn.1004-874X.2011.19.047