Expression of insulin-like growth factor binding protein-3 in HELLP syndrome

• Published in: BMC pregnancy and childbirth Vol. 23; no. 1; pp. 1 - 17
• Main Authors: Wei, Li, Liping, Zhou, Suya, Kang
• Format: Journal Article
• Language: English
• Published: London BioMed Central 10.11.2023; BMC
• Subjects: Apoptosis; Cytokines; Disease; Endothelial cell; Endothelium; Gynecology; HELLP syndrome; Hospitals; Hypoxia; Inflammatory response; Insulin; Insulin-like growth factor binding protein-3 (IGFBP-3); Insulin-like growth factors; Ischemia; Laboratories; Liver; Maternal and Child Health; Medical research; Medicine; Medicine & Public Health; Mortality; Pathogenesis; Placenta; Preeclampsia; Pregnancy; Proteins; Reproductive Medicine; Sample size; Vascular endothelial growth factor; Womens health; United States > US; China; Inflammatory response; Endothelial cell; Insulin-like growth factor binding protein-3 (IGFBP-3); HELLP syndrome
• ISSN: 1471-2393; 1471-2393
• DOI: 10.1186/s12884-023-06074-7

Objective To investigate the expression of insulin-like growth factor binding protein-3(IGFBP-3) in HELLP syndrome and its possible role in the pathogenesis of this disease. Methods 1) 87 subjects were enrolled, including 29 patients with HELLP syndrome, 29 patients with pre-eclampsia (PE), and 29 healthy gravidae as control. The levels of IGFBP-3, IGF-1, TGF-β1, and VEGF in maternal and umbilical blood of them were detected using ELISA. Correlation analysis was used to observe the correlation between IGFBP-3 and IGF-1/TGF-β1/VEGF in maternal and umbilical blood, as well as that between maternal serum IGFBP-3 and clinical diagnostic indicators of HELLP syndrome. 2) Human hepatic sinusoid endothelial cells (HLSEC) and human umbilical vein endothelial cells (HUVEC) were cultured with different concentrations of IGFBP-3. After 72 h of culture, cell apoptosis and the normal living cells rate were detected and compared. Results 1) In both maternal and umbilical blood of HELLP group, levels of IGFBP-3 and TGF-β1 were higher than control and PE group, IGF-1was lower than control group, VEGF was lower than control and PE group. IGFBP-3 in maternal blood was correlated with IGF-1/TGF-β1/ VEGF, while IGFBP-3 in umbilical blood was linked to IGF-1/TGF-β1. In maternal blood, there was a negative correlation between PLT and IGFBP-3, and a positive correlation between ALT/AST/LDH and IGFBP-3. 2) After cultured with IGFBP-3, the total apoptosis rate of either HLSEC or HUVEC was considerably elevated, while the normal living rate was decreased. Conclusion The expression of IGFBP-3 is elevated in HELLP syndrome, which may subsequently promote cell apoptosis by affecting the expression and function of IGF-1, VEGF, and TGFβ1 in the IGF/PI3K/Akt, TGF-β1/Smad3, and VEGF/eNOS/NO pathways. IGFBP-3 aggravates inflammatory reactions of the vascular endothelium and liver under hypoxia, affects the normal function of cells, and plays a role in the pathogenesis of diseases.