西格列汀及胰高血糖素样肽1对脐静脉内皮细胞铁代谢相关基因的调控作用
目的 观察西格列汀及胰高血糖素样肽1（GLP-1）对正常糖浓度（正糖）及高糖条件下人脐静脉内皮细胞（HUVECs）铁代谢相关共济蛋白（FXN）及顺乌头酸梅1（ACO1）蛋白表达的影响.方法 用西格列汀（1μmol/L）和（或）GLP-1（100 nmol/L）处理HUVECs,正糖（5.5 mmol/L）及高糖（30.0 mmol/L）状态下分别设相应对照组、GLP-1处理组、西格列汀处理组以及GLP-1＋西格列汀联合处理组.检测药物对正常状态下HUVECs细胞增殖的影响,用实时定量逆转录PCR和Western blotting法分别于24 h及48 h后检测HUVECs内FXN和ACO1的m...
Saved in:
| Published in | 中华糖尿病杂志 Vol. 9; no. 5; pp. 292 - 297 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
100730,北京医院国家老年医学中心卫生部老年医学重点实验室%100730,北京医院国家老年医学中心 内分泌科
2017
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 1674-5809 |
| DOI | 10.3760/cma.j.issn.1674-5809.2017.05.005 |
Cover
| Abstract | 目的 观察西格列汀及胰高血糖素样肽1（GLP-1）对正常糖浓度（正糖）及高糖条件下人脐静脉内皮细胞（HUVECs）铁代谢相关共济蛋白（FXN）及顺乌头酸梅1（ACO1）蛋白表达的影响.方法 用西格列汀（1μmol/L）和（或）GLP-1（100 nmol/L）处理HUVECs,正糖（5.5 mmol/L）及高糖（30.0 mmol/L）状态下分别设相应对照组、GLP-1处理组、西格列汀处理组以及GLP-1＋西格列汀联合处理组.检测药物对正常状态下HUVECs细胞增殖的影响,用实时定量逆转录PCR和Western blotting法分别于24 h及48 h后检测HUVECs内FXN和ACO1的mRNA及蛋白表达水平.多组间比较采用单因素方差分析,两组间比较使用Bonferroni检验（满足方差齐性检验时）或Dunnett T3检验（不满足方差齐性检验时）.结果 （1）正糖下经西格列汀和（或）GLP-1处理24 h后,与对照组（表达水平设为1）相比,各组的HUVECs细胞增殖水平均无明显变化（分别为0.99±0.04、1.07±0.08、1.06±0.05,F=4.059,均P〉0.05）;而各加药组的FXN mRNA表达均呈下降趋势,其中,西格列汀＋GLP-1组FXN的mRNA表达水平降低为0.68±0.18（t=3.59,P〈0.05）;西格列汀单独或联合GLP-1组ACO1的mRNA表达水平也明显降低（分别为0.49±0.13、0.59±0.15,F=10.802,均P〈0.05）;各加药组的FXN及ACO1的蛋白水平均低于对照组,但差异无统计学意义（均P〉0.05）.（2）高糖下经西格列汀和（或）GLP-1处理48 h后,与对照组（表达水平设为1）相比,西格列汀＋GLP-1组FXN mRNA表达升高（1.75±0.26,t=-5.71,P〈0.05）;但各组的ACO1 mRNA表达变化均不明显且差异无统计学意义（均P〉0.05）.结论 西格列汀和GLP-1可在不影响正常状态内皮细胞增殖的条件下,通过调节正常或高糖状态下HUVECs中FXN及ACO1的表达水平影响细胞内的铁代谢. |
|---|---|
| AbstractList | 目的 观察西格列汀及胰高血糖素样肽1(GLP-1)对正常糖浓度(正糖)及高糖条件下人脐静脉内皮细胞(HUVECs)铁代谢相关共济蛋白(FXN)及顺乌头酸梅1(ACO1)蛋白表达的影响.方法 用西格列汀(1μmol/L)和(或)GLP-1(100 nmol/L)处理HUVECs,正糖(5.5 mmol/L)及高糖(30.0 mmol/L)状态下分别设相应对照组、GLP-1处理组、西格列汀处理组以及GLP-1+西格列汀联合处理组.检测药物对正常状态下HUVECs细胞增殖的影响,用实时定量逆转录PCR和Western blotting法分别于24 h及48 h后检测HUVECs内FXN和ACO1的mRNA及蛋白表达水平.多组间比较采用单因素方差分析,两组间比较使用Bonferroni检验(满足方差齐性检验时)或Dunnett T3检验(不满足方差齐性检验时).结果 (1)正糖下经西格列汀和(或)GLP-1处理24 h后,与对照组(表达水平设为1)相比,各组的HUVECs细胞增殖水平均无明显变化(分别为0.99±0.04、1.07±0.08、1.06±0.05,F=4.059,均P>0.05);而各加药组的FXN mRNA表达均呈下降趋势,其中,西格列汀+GLP-1组FXN的mRNA表达水平降低为0.68±0.18(t=3.59,P<0.05);西格列汀单独或联合GLP-1组ACO1的mRNA表达水平也明显降低(分别为0.49±0.13、0.59±0.15,F=10.802,均P<0.05);各加药组的FXN及ACO1的蛋白水平均低于对照组,但差异无统计学意义(均P>0.05).(2)高糖下经西格列汀和(或)GLP-1处理48 h后,与对照组(表达水平设为1)相比,西格列汀+GLP-1组FXN mRNA表达升高(1.75±0.26,t=-5.71,P<0.05);但各组的ACO1 mRNA表达变化均不明显且差异无统计学意义(均P>0.05).结论 西格列汀和GLP-1可在不影响正常状态内皮细胞增殖的条件下,通过调节正常或高糖状态下HUVECs中FXN及ACO1的表达水平影响细胞内的铁代谢. 目的 观察西格列汀及胰高血糖素样肽1（GLP-1）对正常糖浓度（正糖）及高糖条件下人脐静脉内皮细胞（HUVECs）铁代谢相关共济蛋白（FXN）及顺乌头酸梅1（ACO1）蛋白表达的影响.方法 用西格列汀（1μmol/L）和（或）GLP-1（100 nmol/L）处理HUVECs,正糖（5.5 mmol/L）及高糖（30.0 mmol/L）状态下分别设相应对照组、GLP-1处理组、西格列汀处理组以及GLP-1＋西格列汀联合处理组.检测药物对正常状态下HUVECs细胞增殖的影响,用实时定量逆转录PCR和Western blotting法分别于24 h及48 h后检测HUVECs内FXN和ACO1的mRNA及蛋白表达水平.多组间比较采用单因素方差分析,两组间比较使用Bonferroni检验（满足方差齐性检验时）或Dunnett T3检验（不满足方差齐性检验时）.结果 （1）正糖下经西格列汀和（或）GLP-1处理24 h后,与对照组（表达水平设为1）相比,各组的HUVECs细胞增殖水平均无明显变化（分别为0.99±0.04、1.07±0.08、1.06±0.05,F=4.059,均P〉0.05）;而各加药组的FXN mRNA表达均呈下降趋势,其中,西格列汀＋GLP-1组FXN的mRNA表达水平降低为0.68±0.18（t=3.59,P〈0.05）;西格列汀单独或联合GLP-1组ACO1的mRNA表达水平也明显降低（分别为0.49±0.13、0.59±0.15,F=10.802,均P〈0.05）;各加药组的FXN及ACO1的蛋白水平均低于对照组,但差异无统计学意义（均P〉0.05）.（2）高糖下经西格列汀和（或）GLP-1处理48 h后,与对照组（表达水平设为1）相比,西格列汀＋GLP-1组FXN mRNA表达升高（1.75±0.26,t=-5.71,P〈0.05）;但各组的ACO1 mRNA表达变化均不明显且差异无统计学意义（均P〉0.05）.结论 西格列汀和GLP-1可在不影响正常状态内皮细胞增殖的条件下,通过调节正常或高糖状态下HUVECs中FXN及ACO1的表达水平影响细胞内的铁代谢. |
| Abstract_FL | Objective To investigate the effects of sitagliptin and glucagon like peptide-1 (GLP-1) on the iron metabolism in human umbilical vein endothelial cells (HUVECs) under normal and high glucose conditions. Methods HUVECs were treated with sitagliptin (1μmol/L) and/or GLP-1 (100 nmol/L) under normal (5.5 mmol/L) and high glucose (30.0 mmol/L) conditions, and the corresponding control groups were also included. Cell viability was evaluated. After treatment for 24 h in normal glucose and 48 h in high glucose, mRNA and protein expressions of frataxin (FXN) and aconitase1 (ACO1) were measured by real-time PCR and Western blotting, respectively. Multiple-group comparisons were performed using one-way variance analysis.The two groups were compared using the Bonferroni test (when the variance homogeneity test was performed) or the Dunnett T3 test (when the variance homogeneity test was not satisfied). Results (1) Comparing with the control group (assuming its expression level was 1) under normal glucose condition, cell viability had no significance among those groups treated with GLP-1, sitagliptin and the combinations of sitagliptin and GLP-1 (0.99±0.04, 1.07±0.08, 1.06±0.05,F=4.059, all P>0.05);mRNA expressions of FXN were significantly decreased by combinations of sitagliptin and GLP-1 administration (0.68 ± 0.18, t=3.59, P<0.05) and mRNA expressions of ACO1 were significantly decreased by sitagliptin with or without GLP-1 treatment (0.59 ± 0.15, 0.49 ± 0.13, F=10.802, all P<0.05);While protein expressions of FXN and ACO1 were decreased by sitagliptin and/or GLP-1 with no significance (all P>0.05). (2) Comparing with the control group (assuming its expression level was 1) under high glucose condition, mRNA expressions of FXN were significantly increased by combinations of sitagliptin and GLP-1 administration (1.75 ± 0.26, t=-5.71, P<0.05), while mRNA expressions of ACO1 showed no much significance after treatment with sitagliptin and/or GLP-1. Conclusions Without affecting cell proliferation in normal state of endothelial conditions,sitagliptin and GLP-1 may affect the intracellular iron metabolism by regulating the expression of FXN and ACO1 in HUVECs in normal or hyperglycemic conditions. |
| Author | 李莉 金军华 邹丽辉 张恩毅 张俊华 黄薇 潘琦 肖飞 |
| AuthorAffiliation | 北京医院国家老年医学中心卫生部老年医学重点实验室,100730 北京医院国家老年医学中心内分泌科,100730 |
| AuthorAffiliation_xml | – name: 100730,北京医院国家老年医学中心卫生部老年医学重点实验室%100730,北京医院国家老年医学中心 内分泌科 |
| Author_FL | Zhang Junhua Pan Qi Zhang Enyi Zou Lihui Jin Junhua Huang Wei Xiao Fei Li Li |
| Author_FL_xml | – sequence: 1 fullname: Li Li – sequence: 2 fullname: Jin Junhua – sequence: 3 fullname: Zou Lihui – sequence: 4 fullname: Zhang Enyi – sequence: 5 fullname: Zhang Junhua – sequence: 6 fullname: Huang Wei – sequence: 7 fullname: Pan Qi – sequence: 8 fullname: Xiao Fei |
| Author_xml | – sequence: 1 fullname: 李莉 金军华 邹丽辉 张恩毅 张俊华 黄薇 潘琦 肖飞 |
| BookMark | eNo9kElLw0AcxedQwVr7JTyIl8b_JJlMcpTiBgUvvZdJTbpgU20U0VO1C-pBBcWldYmKKAhaqeBSi1-maabfwpSKpwePH-893ggKWHnLQGgCgyBRBSaTOSZkhYxtWwJWqBwhKmiCCJgKQAQAEkDBf38YhW07owPGGmgAOIgYv__pOt_uzmn3tege7PFSo_d0xm-KXvPEe3O6zjvfbmP35ZOXD3uX57y861YrXu3Za1V56ap3tNVp3fHGrVf_cCtN9_rLrTtercwbpe7-Q6d94R0_jqIhky3ZRvhPQyg-Mx2PzkViC7Pz0alYJElUEmESpSqm_igdqKJQEyQwDFWX1UWZaDKWiWwAlgEzXaRYw6rZhwwdm1QXmWhIITQ-iF1nlsmsVCKbXytYfmFiM71q6f1DgPh3-ODYAEym81ZqJeOjy4VMjhU2EgoVJUXSCJF-AR11iCQ |
| ContentType | Journal Article |
| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| Copyright_xml | – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| DBID | 2RA 92L CQIGP W91 ~WA 2B. 4A8 92I 93N PSX TCJ |
| DOI | 10.3760/cma.j.issn.1674-5809.2017.05.005 |
| DatabaseName | 中文科技期刊数据库 中文科技期刊数据库-CALIS站点 中文科技期刊数据库-7.0平台 中文科技期刊数据库-医药卫生 中文科技期刊数据库- 镜像站点 Wanfang Data Journals - Hong Kong WANFANG Data Centre Wanfang Data Journals 万方数据期刊 - 香港版 China Online Journals (COJ) China Online Journals (COJ) |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| DocumentTitleAlternate | Effects of sitagliptin and glucagon like peptide-1 on the regulations of genes involved in iron metabolism in human umbilical vein endothelial cells |
| DocumentTitle_FL | Effects of sitagliptin and glucagon like peptide-1 on the regulations of genes involved in iron metabolism in human umbilical vein endothelial cells |
| EndPage | 297 |
| ExternalDocumentID | zhtnb201705005 672363955 |
| GrantInformation_xml | – fundername: 国家自然科学基金; 中华医学会临床医学科研专项资金-默沙东糖尿病项目(13020150400) National Natural Science Foundation of China; Chinese Medical Association-MSD Diabetes Research Foundation Joint Clinical Medicine Research Special Program funderid: (81571384,81400036); (81571384, 81400036); (13020150400) |
| GroupedDBID | 2RA 92L ALMA_UNASSIGNED_HOLDINGS CDYEO CQIGP W91 ~WA 2B. 4A8 92I 93N PSX TCJ |
| ID | FETCH-LOGICAL-c585-a377817900b07667f030ee8b48d45941454e01401ab271918f667feb1f7b2a2e3 |
| ISSN | 1674-5809 |
| IngestDate | Thu May 29 04:06:42 EDT 2025 Wed Feb 14 09:59:53 EST 2024 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 5 |
| Keywords | Human umbilical vein endothelial cells 顺乌头酸梅1 Aconitase1 西格列汀 胰高血糖素样肽1 Frataxin Sitagliptin 人脐静脉内皮细胞 Glucagon like peptide-1 共济蛋白 |
| Language | Chinese |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c585-a377817900b07667f030ee8b48d45941454e01401ab271918f667feb1f7b2a2e3 |
| Notes | Sitagliptin;Frataxin;Human umbilical vein endothelial cells Objective To investigate the effects of sitagliptin and glucagon like peptide-1 （GLP-1） on the iron metabolism in human umbilical vein endothelial cells （HUVECs） under normal and high glucose conditions. Methods HUVECs were treated with sitagliptin （1μmol/L） and/or GLP-1 （100 nmol/L） under normal （5.5 mmol/L） and high glucose （30.0 mmol/L） conditions, and the corresponding control groups were also included. Cell viability was evaluated. After treatment for 24 h in normal glucose and 48 h in high glucose, mRNA and protein expressions of frataxin （FXN） and aconitase1 （ACO1） were measured by real-time PCR and Western blotting, respectively. Multiple-group comparisons were performed using one-way variance analysis.The two groups were compared using the Bonferroni test （when the variance homogeneity test was performed） or the Dunnett T3 test （when the variance homogeneity test was not satisfied）. Results （1） Comparing with the control group （assuming its e |
| PageCount | 6 |
| ParticipantIDs | wanfang_journals_zhtnb201705005 chongqing_primary_672363955 |
| PublicationCentury | 2000 |
| PublicationDate | 2017 |
| PublicationDateYYYYMMDD | 2017-01-01 |
| PublicationDate_xml | – year: 2017 text: 2017 |
| PublicationDecade | 2010 |
| PublicationTitle | 中华糖尿病杂志 |
| PublicationTitleAlternate | CHINESE JOURNAL OF DIABETES MELLITUS |
| PublicationTitle_FL | Chinese Journal of Diabetes Mellitus |
| PublicationYear | 2017 |
| Publisher | 100730,北京医院国家老年医学中心卫生部老年医学重点实验室%100730,北京医院国家老年医学中心 内分泌科 |
| Publisher_xml | – name: 100730,北京医院国家老年医学中心卫生部老年医学重点实验室%100730,北京医院国家老年医学中心 内分泌科 |
| SSID | ssib011909001 ssib048413644 ssib007286532 ssib017477303 ssib003003870 ssib051368295 |
| Score | 2.0890203 |
| Snippet | 目的 观察西格列汀及胰高血糖素样肽1（GLP-1）对正常糖浓度（正糖）及高糖条件下人脐静脉内皮细胞（HUVECs）铁代谢相关共济蛋白（FXN）及顺乌头酸梅1（ACO1）蛋白表达的影... 目的 观察西格列汀及胰高血糖素样肽1(GLP-1)对正常糖浓度(正糖)及高糖条件下人脐静脉内皮细胞(HUVECs)铁代谢相关共济蛋白(FXN)及顺乌头酸梅1(ACO1)蛋白表达的影响.方法 用... |
| SourceID | wanfang chongqing |
| SourceType | Aggregation Database Publisher |
| StartPage | 292 |
| SubjectTerms | 人脐静脉内皮细胞 共济蛋白 胰高血糖素样肽1 西格列汀 顺乌头酸梅1 |
| Title | 西格列汀及胰高血糖素样肽1对脐静脉内皮细胞铁代谢相关基因的调控作用 |
| URI | http://lib.cqvip.com/qk/71184X/201705/672363955.html https://d.wanfangdata.com.cn/periodical/zhtnb201705005 |
| Volume | 9 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| journalDatabaseRights | – providerCode: PRVEBS databaseName: EBSCOhost Academic Search Ultimate issn: 1674-5809 databaseCode: ABDBF dateStart: 20150701 customDbUrl: https://search.ebscohost.com/login.aspx?authtype=ip,shib&custid=s3936755&profile=ehost&defaultdb=asn isFulltext: true dateEnd: 99991231 titleUrlDefault: https://search.ebscohost.com/direct.asp?db=asn omitProxy: true ssIdentifier: ssib011909001 providerName: EBSCOhost |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1ba9RAFA69gPgiioq1XvrggFC2ZpJMZuZx0s1SBH2q0LeS7GZbBLdeti99qvaC-qCC4qX1siqiUNBKBS-1-Ge6u-m_8JxJNhtFvL2E5Myc23eSnDNhZmIYJ8KqCCMamgWzwmCAwkRYgGFQtSCDslm2q7QcSVycfOasO3bOOT3BJnp613Kzlmbr4Uh57pfrSv4nqkCDuOIq2X-IbCYUCHAO8YUjRBiOfxVj4guiGPFKxHeJMok3SnxGhCCSI8WjOI8BKSUiFHYWNvGAIonyiBSaPenDiWcT6eoTB0WlArnmsohXpChIlYgnNckhUguSRSI7FCG1MpcIhoKkIsrXEj0kJuqlr7lsqGCJ72CTsrEJzFKW5vKIJ7QchjbBiQSlSp942jItGdQlXCATbBU-UVwLBHtGdR9wQ-RLb90KDhe18CKydN1mKApx5Igd2u-ia-C5r_GVWbrutPjaIR-cHkaPBDDS1H2ws6MiabM0alo92Id2p3wMQ5agDXgoqWGHYLF8m4MGCPWjTMRUQwDWCz6sw13UMeUYGeUOp5FD7ySCLP38F55kKWuajlzuFJgwZT5fydxjyfK5J_mpYFrGWAkwP2dInAMFt3X5QjByXusYyXTgPMdkG1uTdauDbM6myy0bylnGeo1-i0NZ12f0K6_olXLpAudCdF__HFc9d5ctU6g9ZWeWA17DSBbyS5YeHAEFldutNhlcCUv_NymzcZdxMnXi1J9cwM1SpmdqU5egBNQr8mrVoDaVKx7H9xp70lHfkEoe4X1Gz9z0fiOIX31rNb42rz9ovZ9v3r4ZL6zvrD2Mn8-3N-63PzRajY_xtS3afPc5Xryz8-RRvHijubzUXnnb3lyOF57u3L26vfkyXn_RXv3UXNpoPvvSXG20Vxbj9YXWrdfbW4_b994cMMZL_vjoWCH940mhDMP2QmBzLnDLPDM0AWFeBUijSISOqDhMOtRhTqS_iAShxamkooqdoNqq8tAKrMg-aPTVZmrRIWMocCKL0goNy7ianFLBaaUCwzlpRkxEVXvAGMzQmbyYbGwzmYV4wDie4jWZvu6uTM5N12uhpbffAnQP_5Z_0NiNPZNPlUeMvvrl2egoFO_18Fh603wHlyO8OQ |
| linkProvider | EBSCOhost |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E8%A5%BF%E6%A0%BC%E5%88%97%E6%B1%80%E5%8F%8A%E8%83%B0%E9%AB%98%E8%A1%80%E7%B3%96%E7%B4%A0%E6%A0%B7%E8%82%BD1%E5%AF%B9%E8%84%90%E9%9D%99%E8%84%89%E5%86%85%E7%9A%AE%E7%BB%86%E8%83%9E%E9%93%81%E4%BB%A3%E8%B0%A2%E7%9B%B8%E5%85%B3%E5%9F%BA%E5%9B%A0%E7%9A%84%E8%B0%83%E6%8E%A7%E4%BD%9C%E7%94%A8&rft.jtitle=%E4%B8%AD%E5%8D%8E%E7%B3%96%E5%B0%BF%E7%97%85%E6%9D%82%E5%BF%97&rft.au=%E6%9D%8E%E8%8E%89+%E9%87%91%E5%86%9B%E5%8D%8E+%E9%82%B9%E4%B8%BD%E8%BE%89+%E5%BC%A0%E6%81%A9%E6%AF%85+%E5%BC%A0%E4%BF%8A%E5%8D%8E+%E9%BB%84%E8%96%87+%E6%BD%98%E7%90%A6+%E8%82%96%E9%A3%9E&rft.date=2017&rft.issn=1674-5809&rft.volume=9&rft.issue=5&rft.spage=292&rft.epage=297&rft_id=info:doi/10.3760%2Fcma.j.issn.1674-5809.2017.05.005&rft.externalDocID=672363955 |
| thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fimage.cqvip.com%2Fvip1000%2Fqk%2F71184X%2F71184X.jpg http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Fzhtnb%2Fzhtnb.jpg |