电化学免疫传感器快速检测农产品中的毒死蜱

研究了一种无标记的电化学免疫传感器，用于农产品中的毒死蜱农药残留的快速检测。将毒死蜱人工抗原作为生物识别元件固定在金电极的表面，采用间接竞争法原理，样品中的被测组分与电极上的固定化包被抗原竞争性结合溶液中的抗体。抗体抗原结合反应通过电化学阻抗谱和石英晶体微天平进行表征。将该免疫传感器用于检测青菜、苹果等农产品中的毒死蜱农药残留。结果表明，此免疫传感器灵敏度好、准确度高；对毒死蜱农药的检测限为0.01μg/mL，回收率大于85%，检测时间小于1 h，变异系数小于5%，传感器经过再生处理后能重复使用，经济性较好。该研究可为实现快速检测农产品中农药残留传感器的商品化提供参考。...

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Bibliographic Details
Published in农业工程学报 Vol. 30; no. 12; pp. 278 - 283
Main Author 蒋雪松 王维琴 卢利群 郑丹丹 陈卉卉 许林云
Format Journal Article
LanguageChinese
Published 南京林业大学机械电子工程学院，南京 210037 2014
宁波海通食品科技有限公司，宁波 315300%宁波海通食品科技有限公司,宁波,315300%南京林业大学机械电子工程学院,南京,210037
Subjects
免疫传感器
农产品
农药
微天平
检测
毒死蜱
电化学阻抗谱
电化学阻抗谱
chlorphrifos
detection
microbalance
检测
农产品
毒死蜱
微天平
immunosensors
agriculture products
impedance spectroscopy
免疫传感器
pesticides
农药
pesticide residues
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ISSN1002-6819
DOI10.3969/j.issn.1002-6819.2014.12.035

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Summary:研究了一种无标记的电化学免疫传感器，用于农产品中的毒死蜱农药残留的快速检测。将毒死蜱人工抗原作为生物识别元件固定在金电极的表面，采用间接竞争法原理，样品中的被测组分与电极上的固定化包被抗原竞争性结合溶液中的抗体。抗体抗原结合反应通过电化学阻抗谱和石英晶体微天平进行表征。将该免疫传感器用于检测青菜、苹果等农产品中的毒死蜱农药残留。结果表明，此免疫传感器灵敏度好、准确度高；对毒死蜱农药的检测限为0.01μg/mL，回收率大于85%，检测时间小于1 h，变异系数小于5%，传感器经过再生处理后能重复使用，经济性较好。该研究可为实现快速检测农产品中农药残留传感器的商品化提供参考。
Bibliography:11-2047/S
immunosensors;pesticides;agriculture products;chlorphrifos;detection;impedance spectroscopy;pesticide residues;microbalance
The objective of this study was to develop a label free electrochemical immunosensor for detection of pesticide chlorpyrifos （CPF） in agricultural products. The surface modification of sensors consisted of three successive steps：1） surface cleaning;2） increasing the surface coverage of hydroxyl groups;3） silanization. The CPF hapten conjugate used as recognizing elements were immobilized on the surface of an electrode. Cyclic votammetry was investigated before and after hapten coating, which that confirmed CPF hapten could be successfully coated on the electrodes. The spiked CPF solution was diluted into various folds and mixed with an CPF antibody solution. The detection method was based on an indirect competitive reaction of a limited anti-CPF polyclonal antibody in a sample solution with immobilized hapten, and with a pesticide analyte in the sample solution. The antibody-anti
ISSN:1002-6819
DOI:10.3969/j.issn.1002-6819.2014.12.035