Ydj1p锌指结构突变体对底物结合影响的分子动力学模拟分析

Ydj1p是酵母细胞质中一种主要的I型Hsp40分子伴侣,Ydj1p锌指结构在传递底物给Hsp70时发挥重要的作用,锌指结构域的两个锌离子结合位点区域（ZBDⅠ和ZBDⅡ）与半胱氨酸形成配位键对底物传递中维持结构稳定非常重要。本研究通过分子动力学手段对Ydj1p与各锌指结构突变体进行了模拟,分析ZBDⅠ突变体关键残基C143S、C201S,ZBDⅡ突变体关键残基C162S、C185S的突变影响Hsp40与Hsp70的底物传递。分析结果表明,当锌指部位的氨基酸发生突变,不仅能影响Ydj1p的结构稳定性,也能影响底物的传递,并且锌指结构Ⅰ突变体和锌指结构Ⅱ突变体之间也具有明显差异。通过结合能量的分...

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Bibliographic Details
Published in生物信息学 Vol. 15; no. 2; pp. 90 - 96
Main Author 刘冠宏 孙玉娜 张桥石 薛友林 宋有涛
Format Journal Article
LanguageChinese
Published 辽宁大学环境学院,沈阳,110036%辽宁大学生命学院,沈阳,110036%辽宁大学轻型产业学院,沈阳,110036%辽宁大学环境学院,沈阳110036 2017
辽宁大学生命学院,沈阳110036
Subjects
Ydj1p
分子动力学模拟
底物结合
突变体
锌指结构
Zinc binding domain
底物结合
Molecular dynamic
锌指结构
突变体
Ydj1p
分子动力学模拟
Mutants
Substrate binding
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ISSN1672-5565
DOI10.3969/j.issn.1672-5565.20160715001

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Summary:Ydj1p是酵母细胞质中一种主要的I型Hsp40分子伴侣,Ydj1p锌指结构在传递底物给Hsp70时发挥重要的作用,锌指结构域的两个锌离子结合位点区域（ZBDⅠ和ZBDⅡ）与半胱氨酸形成配位键对底物传递中维持结构稳定非常重要。本研究通过分子动力学手段对Ydj1p与各锌指结构突变体进行了模拟,分析ZBDⅠ突变体关键残基C143S、C201S,ZBDⅡ突变体关键残基C162S、C185S的突变影响Hsp40与Hsp70的底物传递。分析结果表明,当锌指部位的氨基酸发生突变,不仅能影响Ydj1p的结构稳定性,也能影响底物的传递,并且锌指结构Ⅰ突变体和锌指结构Ⅱ突变体之间也具有明显差异。通过结合能量的分析以及构象变化比对,揭示了Ydj1p以及各锌指结构突变体底物结合能力的强弱,这与生化实验研究了Ydj1p锌指结构与Hsp70合作,帮助多肽传递的功能是至关重要的结果较为相近。
Bibliography:Ydj1p ; Zinc binding domain ; Mutants ; Substrate binding ; Molecular dynamic
Ydj1p is a major kind of I type Hsp40 molecular chaperone in yeast cytoplasm. Zinc finger structure plays a significant role in transmitting substrates to Hsp70. The two zinc binding sites region （ZBD I and ZBD II） in the zinc finger domain can form a coordination bond with cysteine, which is important for maintaining structural stability during the substrate transfer process. In this paper, Ydj l p and the various zinc structural mutants are studied by using the method of molecular dynamic simulation. How the mutations that take place in ZBD I mutant key residues C143S, C201S and ZBD II mutant key residues C162s, C185S affect the substrate transfer between Hsp40 and HspT0 are also analyzed. The results show that mutations in the amino acids in zinc finger sites not only affect the structural stability of Ydjlp, but also affect the substrate transport. Moreover, there are also significant differences between zinc finger type I mutant
ISSN:1672-5565
DOI:10.3969/j.issn.1672-5565.20160715001