Analysis of Circadian Clock Gene Expression in Human Skin Explants
Many aspects of skin biochemistry and physiology are known to vary over the course of the 24-hour day. Traditional approaches to study circadian rhythms in the skin have employed rodents or human subjects, which limit the experimental variables that can be studied. Although explants derived from dis...
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Published in | JID innovations Vol. 4; no. 6; p. 100308 |
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Main Authors | , |
Format | Journal Article |
Language | English |
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01.11.2024
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ISSN | 2667-0267 2667-0267 |
DOI | 10.1016/j.xjidi.2024.100308 |
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Abstract | Many aspects of skin biochemistry and physiology are known to vary over the course of the 24-hour day. Traditional approaches to study circadian rhythms in the skin have employed rodents or human subjects, which limit the experimental variables that can be studied. Although explants derived from discarded surgical skin are a commonly used model in the skin biology field, circadian rhythms have yet to be examined ex vivo. In this study, using human panniculectomy skin, we used RT-qPCR to monitor the epidermal expression of 4 core circadian clock genes over the course of 1 day ex vivo. Although significant interindividual variability in overall gene expression profiles was observed, robust circadian oscillations were observed in many of the genes and individual explants. Comparison of our gene expression data with microarray data from 2 previous human-subject studies involving primarily young adult White males revealed both similarities and differences, including greater distribution in the time of day of peak expression in the skin explants. This increased variability appears to be due in part to the increased age and altered sex distribution of the donated skin. Nonetheless, our results indicate that skin explants offer an additional experimental system for studying circadian skin biology. |
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AbstractList | Many aspects of skin biochemistry and physiology are known to vary over the course of the 24-hour day. Traditional approaches to study circadian rhythms in the skin have employed rodents or human subjects, which limit the experimental variables that can be studied. Although explants derived from discarded surgical skin are a commonly used model in the skin biology field, circadian rhythms have yet to be examined ex vivo. In this study, using human panniculectomy skin, we used RT-qPCR to monitor the epidermal expression of 4 core circadian clock genes over the course of 1 day ex vivo. Although significant interindividual variability in overall gene expression profiles was observed, robust circadian oscillations were observed in many of the genes and individual explants. Comparison of our gene expression data with microarray data from 2 previous human-subject studies involving primarily young adult White males revealed both similarities and differences, including greater distribution in the time of day of peak expression in the skin explants. This increased variability appears to be due in part to the increased age and altered sex distribution of the donated skin. Nonetheless, our results indicate that skin explants offer an additional experimental system for studying circadian skin biology. Many aspects of skin biochemistry and physiology are known to vary over the course of the 24-hour day. Traditional approaches to study circadian rhythms in the skin have employed rodents or human subjects, which limit the experimental variables that can be studied. Although explants derived from discarded surgical skin are a commonly used model in the skin biology field, circadian rhythms have yet to be examined ex vivo. In this study, using human panniculectomy skin, we used RT-qPCR to monitor the epidermal expression of 4 core circadian clock genes over the course of 1 day ex vivo. Although significant interindividual variability in overall gene expression profiles was observed, robust circadian oscillations were observed in many of the genes and individual explants. Comparison of our gene expression data with microarray data from 2 previous human-subject studies involving primarily young adult White males revealed both similarities and differences, including greater distribution in the time of day of peak expression in the skin explants. This increased variability appears to be due in part to the increased age and altered sex distribution of the donated skin. Nonetheless, our results indicate that skin explants offer an additional experimental system for studying circadian skin biology.Many aspects of skin biochemistry and physiology are known to vary over the course of the 24-hour day. Traditional approaches to study circadian rhythms in the skin have employed rodents or human subjects, which limit the experimental variables that can be studied. Although explants derived from discarded surgical skin are a commonly used model in the skin biology field, circadian rhythms have yet to be examined ex vivo. In this study, using human panniculectomy skin, we used RT-qPCR to monitor the epidermal expression of 4 core circadian clock genes over the course of 1 day ex vivo. Although significant interindividual variability in overall gene expression profiles was observed, robust circadian oscillations were observed in many of the genes and individual explants. Comparison of our gene expression data with microarray data from 2 previous human-subject studies involving primarily young adult White males revealed both similarities and differences, including greater distribution in the time of day of peak expression in the skin explants. This increased variability appears to be due in part to the increased age and altered sex distribution of the donated skin. Nonetheless, our results indicate that skin explants offer an additional experimental system for studying circadian skin biology. |
ArticleNumber | 100308 |
Author | Cvammen, William Kemp, Michael G. |
Author_xml | – sequence: 1 givenname: William orcidid: 0000-0002-6417-905X surname: Cvammen fullname: Cvammen, William organization: Department of Pharmacology & Toxicology, Boonshoft School of Medicine, Wright State University, Dayton, Ohio, USA – sequence: 2 givenname: Michael G. orcidid: 0000-0001-8203-0745 surname: Kemp fullname: Kemp, Michael G. email: mike.kemp@wright.edu organization: Department of Pharmacology & Toxicology, Boonshoft School of Medicine, Wright State University, Dayton, Ohio, USA |
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Cites_doi | 10.1177/0748730405277232 10.1016/j.clnu.2021.01.015 10.1111/jpi.12956 10.1159/000533261 10.1038/nrg.2016.150 10.1371/journal.pgen.1000442 10.1016/S0092-8674(00)81199-X 10.1016/j.drudis.2021.03.015 10.1126/science.289.5488.2344 10.1093/bioinformatics/btw405 10.1073/pnas.1118641109 10.1016/j.jid.2024.05.010 10.1093/nar/gkv007 10.1007/s13105-015-0447-3 10.1093/advances/nmac093 10.1093/bioinformatics/btq431 10.1093/bioinformatics/btg405 10.1038/nsmb925 10.1073/pnas.1809442115 10.18632/oncotarget.15074 10.3390/ijms22116112 10.1146/annurev-neuro-060909-153128 10.1002/1873-3468.14192 10.1016/j.cmet.2016.02.007 10.1146/annurev-pharmtox-010617-052645 10.1177/0261192920914193 10.3390/ijms21020674 10.1177/0748730414563537 |
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Keywords | Circadian clock UVR Gene expression Skin explant |
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References | (bib14) 2023 Akashi, Takumi (bib1) 2005; 12 Speksnijder, Bisschop, Siegelaar, Stenvers, Kalsbeek (bib24) 2024; 76 Camello-Almaraz, Martin-Cano, Santos, Espin, Antonio Madrid, Pozo (bib5) 2020; 21 Chen, Yoo, Takahashi (bib7) 2018; 58 Eberlin, Silva, Facchini, Silva, Pinheiro, Eberlin (bib12) 2020; 48 Ritchie, Phipson, Wu, Hu, Law, Shi (bib23) 2015; 43 Cvammen, Dean Rider, Kemp (bib9) 2024 Lee, Bova, Schofield, Bryant, Dieckmann, Slattery (bib18) 2016; 23 Lubov, Cvammen, Kemp (bib19) 2021; 22 Carvalho, Irizarry (bib6) 2010; 26 Mohawk, Green, Takahashi (bib20) 2012; 35 Balsalobre, Brown, Marcacci, Tronche, Kellendonk, Reichardt (bib2) 2000; 289 Guillaumond, Dardente, Giguère, Cermakian (bib15) 2005; 20 Cousin, Misery, de Vries, Lebonvallet (bib8) 2023; 239 Takahashi (bib26) 2017; 18 van der Merwe, Münch, Kruger (bib28) 2022; 13 Spörl, Korge, Jürchott, Wunderskirchner, Schellenberg, Heins (bib25) 2012; 109 Gautier, Cope, Bolstad, Irizarry (bib13) 2004; 20 Balsalobre, Damiola, Schibler (bib3) 1998; 93 Ribeiro, Cavadas, Silva (bib22) 2021; 26 Wu, Ruben, Schmidt, Francey, Smith, Anafi (bib30) 2018; 115 Duan, Greenberg, Karri, Andersen (bib11) 2021; 595 Benna, Helfrich-Förster, Rajendran, Monticelli, Pilati, Nitti (bib4) 2017; 8 Plikus, Van Spyk, Pham, Geyfman, Kumar, Takahashi (bib21) 2015; 30 Del Olmo, Spörl, Korge, Jürchott, Felten, Grudziecki (bib10) 2022; 4 Lago-Sampedro, Ho-Plagaro, Garcia-Serrano, Santiago-Fernandez, Rodríguez-Díaz, Lopez-Gómez (bib17) 2021; 40 Hughes, DiTacchio, Hayes, Vollmers, Pulivarthy, Baggs (bib16) 2009; 5 Valladares, Obregón, Chaput (bib27) 2015; 71 Wu, Anafi, Hughes, Kornacker, Hogenesch (bib29) 2016; 32 Lago-Sampedro (10.1016/j.xjidi.2024.100308_bib17) 2021; 40 van der Merwe (10.1016/j.xjidi.2024.100308_bib28) 2022; 13 Del Olmo (10.1016/j.xjidi.2024.100308_bib10) 2022; 4 Carvalho (10.1016/j.xjidi.2024.100308_bib6) 2010; 26 Plikus (10.1016/j.xjidi.2024.100308_bib21) 2015; 30 Hughes (10.1016/j.xjidi.2024.100308_bib16) 2009; 5 Balsalobre (10.1016/j.xjidi.2024.100308_bib3) 1998; 93 Lee (10.1016/j.xjidi.2024.100308_bib18) 2016; 23 Speksnijder (10.1016/j.xjidi.2024.100308_bib24) 2024; 76 Takahashi (10.1016/j.xjidi.2024.100308_bib26) 2017; 18 Ritchie (10.1016/j.xjidi.2024.100308_bib23) 2015; 43 Cvammen (10.1016/j.xjidi.2024.100308_bib9) 2024 Balsalobre (10.1016/j.xjidi.2024.100308_bib2) 2000; 289 Eberlin (10.1016/j.xjidi.2024.100308_bib12) 2020; 48 Benna (10.1016/j.xjidi.2024.100308_bib4) 2017; 8 Mohawk (10.1016/j.xjidi.2024.100308_bib20) 2012; 35 Guillaumond (10.1016/j.xjidi.2024.100308_bib15) 2005; 20 Chen (10.1016/j.xjidi.2024.100308_bib7) 2018; 58 Cousin (10.1016/j.xjidi.2024.100308_bib8) 2023; 239 Camello-Almaraz (10.1016/j.xjidi.2024.100308_bib5) 2020; 21 Duan (10.1016/j.xjidi.2024.100308_bib11) 2021; 595 Valladares (10.1016/j.xjidi.2024.100308_bib27) 2015; 71 Wu (10.1016/j.xjidi.2024.100308_bib30) 2018; 115 Lubov (10.1016/j.xjidi.2024.100308_bib19) 2021; 22 Ribeiro (10.1016/j.xjidi.2024.100308_bib22) 2021; 26 Akashi (10.1016/j.xjidi.2024.100308_bib1) 2005; 12 Gautier (10.1016/j.xjidi.2024.100308_bib13) 2004; 20 Wu (10.1016/j.xjidi.2024.100308_bib29) 2016; 32 Spörl (10.1016/j.xjidi.2024.100308_bib25) 2012; 109 |
References_xml | – volume: 58 start-page: 231 year: 2018 end-page: 252 ident: bib7 article-title: Development and therapeutic potential of small-molecule modulators of circadian systems publication-title: Annu Rev Pharmacol Toxicol – year: 2024 ident: bib9 article-title: Analysis of circadian clock gene expression in epidermal human skin indicates that body location impacts rhythm amplitude [e-pub ahead of print] publication-title: J Invest Dermatol – volume: 8 start-page: 23978 year: 2017 end-page: 23995 ident: bib4 article-title: Genetic variation of clock genes and cancer risk: a field synopsis and meta-analysis publication-title: Oncotarget – volume: 4 year: 2022 ident: bib10 article-title: Inter-layer and inter-subject variability of diurnal gene expression in human skin publication-title: NAR Genom Bioinform – volume: 595 start-page: 2413 year: 2021 end-page: 2436 ident: bib11 article-title: The circadian clock and diseases of the skin publication-title: FEBS Lett – volume: 20 start-page: 307 year: 2004 end-page: 315 ident: bib13 article-title: affy--analysis of Affymetrix GeneChip data at the probe level publication-title: Bioinformatics – volume: 239 start-page: 849 year: 2023 end-page: 859 ident: bib8 article-title: Emergence of new concepts in skin physiopathology through the use of in vitro human skin explants models publication-title: Dermatology – volume: 20 start-page: 391 year: 2005 end-page: 403 ident: bib15 article-title: Differential control of Bmal1 circadian transcription by REV-ERB and ROR nuclear receptors publication-title: J Biol Rhythms – volume: 21 start-page: 1 year: 2020 end-page: 13 ident: bib5 article-title: Age-induced differential changes in the central and colonic human circadian oscillators publication-title: Int J Mol Sci – volume: 115 start-page: 12313 year: 2018 end-page: 12318 ident: bib30 article-title: Population-level rhythms in human skin with implications for circadian medicine publication-title: Proc Natl Acad Sci USA – volume: 23 start-page: 602 year: 2016 end-page: 609 ident: bib18 article-title: Brown adipose tissue exhibits a glucose-responsive thermogenic biorhythm in humans publication-title: Cell Metab – volume: 76 year: 2024 ident: bib24 article-title: Circadian desynchrony and glucose metabolism publication-title: J Pineal Res – volume: 93 start-page: 929 year: 1998 end-page: 937 ident: bib3 article-title: A serum shock induces circadian gene expression in mammalian tissue culture cells publication-title: Cell – volume: 12 start-page: 441 year: 2005 end-page: 448 ident: bib1 article-title: The orphan nuclear receptor RORalpha regulates circadian transcription of the mammalian core-clock Bmal1 publication-title: Nat Struct Mol Biol – volume: 5 year: 2009 ident: bib16 article-title: Harmonics of circadian gene transcription in mammals publication-title: PLoS Genet – volume: 32 start-page: 3351 year: 2016 end-page: 3353 ident: bib29 article-title: MetaCycle: an integrated R package to evaluate periodicity in large scale data publication-title: Bioinformatics – volume: 48 start-page: 10 year: 2020 end-page: 22 ident: bib12 article-title: The ex vivo skin model as an alternative tool for the efficacy and safety evaluation of topical products publication-title: Altern Lab Anim – volume: 30 start-page: 163 year: 2015 end-page: 182 ident: bib21 article-title: The circadian clock in skin: implications for adult stem cells, tissue regeneration, cancer, aging, and immunity publication-title: J Biol Rhythms – volume: 26 start-page: 1620 year: 2021 end-page: 1641 ident: bib22 article-title: Small-molecule modulators of the circadian clock: pharmacological potentials in circadian-related diseases publication-title: Drug Discov Today – volume: 40 start-page: 4324 year: 2021 end-page: 4333 ident: bib17 article-title: Oleic acid restores the rhythmicity of the disrupted circadian rhythm found in gastrointestinal explants from patients with morbid obesity publication-title: Clin Nutr – volume: 26 start-page: 2363 year: 2010 end-page: 2367 ident: bib6 article-title: A framework for oligonucleotide microarray preprocessing publication-title: Bioinformatics – volume: 43 start-page: e47 year: 2015 ident: bib23 article-title: limma powers differential expression analyses for RNA-sequencing and microarray studies publication-title: Nucleic Acids Res – volume: 109 start-page: 10903 year: 2012 end-page: 10908 ident: bib25 article-title: Krüppel-like factor 9 is a circadian transcription factor in human epidermis that controls proliferation of keratinocytes publication-title: Proc Natl Acad Sci USA – year: 2023 ident: bib14 article-title: GraphPad Prism – volume: 13 start-page: 2357 year: 2022 end-page: 2405 ident: bib28 article-title: Chronotype differences in body composition, dietary intake and eating behavior outcomes: a scoping systematic review publication-title: Adv Nutr – volume: 71 start-page: 855 year: 2015 end-page: 860 ident: bib27 article-title: Association between genetic variants of the clock gene and obesity and sleep duration publication-title: J Physiol Biochem – volume: 18 start-page: 164 year: 2017 end-page: 179 ident: bib26 article-title: Transcriptional architecture of the mammalian circadian clock publication-title: Nat Rev Genet – volume: 35 start-page: 445 year: 2012 end-page: 462 ident: bib20 article-title: Central and peripheral circadian clocks in mammals publication-title: Annu Rev Neurosci – volume: 289 start-page: 2344 year: 2000 end-page: 2347 ident: bib2 article-title: Resetting of circadian time in peripheral tissues by glucocorticoid signaling publication-title: Science – volume: 22 start-page: 1 year: 2021 end-page: 18 ident: bib19 article-title: The impact of the circadian clock on skin physiology and cancer development publication-title: Int J Mol Sci – volume: 20 start-page: 391 year: 2005 ident: 10.1016/j.xjidi.2024.100308_bib15 article-title: Differential control of Bmal1 circadian transcription by REV-ERB and ROR nuclear receptors publication-title: J Biol Rhythms doi: 10.1177/0748730405277232 – volume: 40 start-page: 4324 year: 2021 ident: 10.1016/j.xjidi.2024.100308_bib17 article-title: Oleic acid restores the rhythmicity of the disrupted circadian rhythm found in gastrointestinal explants from patients with morbid obesity publication-title: Clin Nutr doi: 10.1016/j.clnu.2021.01.015 – volume: 76 year: 2024 ident: 10.1016/j.xjidi.2024.100308_bib24 article-title: Circadian desynchrony and glucose metabolism publication-title: J Pineal Res doi: 10.1111/jpi.12956 – volume: 239 start-page: 849 year: 2023 ident: 10.1016/j.xjidi.2024.100308_bib8 article-title: Emergence of new concepts in skin physiopathology through the use of in vitro human skin explants models publication-title: Dermatology doi: 10.1159/000533261 – volume: 18 start-page: 164 year: 2017 ident: 10.1016/j.xjidi.2024.100308_bib26 article-title: Transcriptional architecture of the mammalian circadian clock publication-title: Nat Rev Genet doi: 10.1038/nrg.2016.150 – volume: 5 year: 2009 ident: 10.1016/j.xjidi.2024.100308_bib16 article-title: Harmonics of circadian gene transcription in mammals publication-title: PLoS Genet doi: 10.1371/journal.pgen.1000442 – volume: 93 start-page: 929 year: 1998 ident: 10.1016/j.xjidi.2024.100308_bib3 article-title: A serum shock induces circadian gene expression in mammalian tissue culture cells publication-title: Cell doi: 10.1016/S0092-8674(00)81199-X – volume: 26 start-page: 1620 year: 2021 ident: 10.1016/j.xjidi.2024.100308_bib22 article-title: Small-molecule modulators of the circadian clock: pharmacological potentials in circadian-related diseases publication-title: Drug Discov Today doi: 10.1016/j.drudis.2021.03.015 – volume: 289 start-page: 2344 year: 2000 ident: 10.1016/j.xjidi.2024.100308_bib2 article-title: Resetting of circadian time in peripheral tissues by glucocorticoid signaling publication-title: Science doi: 10.1126/science.289.5488.2344 – volume: 4 year: 2022 ident: 10.1016/j.xjidi.2024.100308_bib10 article-title: Inter-layer and inter-subject variability of diurnal gene expression in human skin publication-title: NAR Genom Bioinform – volume: 32 start-page: 3351 year: 2016 ident: 10.1016/j.xjidi.2024.100308_bib29 article-title: MetaCycle: an integrated R package to evaluate periodicity in large scale data publication-title: Bioinformatics doi: 10.1093/bioinformatics/btw405 – volume: 109 start-page: 10903 year: 2012 ident: 10.1016/j.xjidi.2024.100308_bib25 article-title: Krüppel-like factor 9 is a circadian transcription factor in human epidermis that controls proliferation of keratinocytes publication-title: Proc Natl Acad Sci USA doi: 10.1073/pnas.1118641109 – year: 2024 ident: 10.1016/j.xjidi.2024.100308_bib9 article-title: Analysis of circadian clock gene expression in epidermal human skin indicates that body location impacts rhythm amplitude [e-pub ahead of print] publication-title: J Invest Dermatol doi: 10.1016/j.jid.2024.05.010 – volume: 43 start-page: e47 year: 2015 ident: 10.1016/j.xjidi.2024.100308_bib23 article-title: limma powers differential expression analyses for RNA-sequencing and microarray studies publication-title: Nucleic Acids Res doi: 10.1093/nar/gkv007 – volume: 71 start-page: 855 year: 2015 ident: 10.1016/j.xjidi.2024.100308_bib27 article-title: Association between genetic variants of the clock gene and obesity and sleep duration publication-title: J Physiol Biochem doi: 10.1007/s13105-015-0447-3 – volume: 13 start-page: 2357 year: 2022 ident: 10.1016/j.xjidi.2024.100308_bib28 article-title: Chronotype differences in body composition, dietary intake and eating behavior outcomes: a scoping systematic review publication-title: Adv Nutr doi: 10.1093/advances/nmac093 – volume: 26 start-page: 2363 year: 2010 ident: 10.1016/j.xjidi.2024.100308_bib6 article-title: A framework for oligonucleotide microarray preprocessing publication-title: Bioinformatics doi: 10.1093/bioinformatics/btq431 – volume: 20 start-page: 307 year: 2004 ident: 10.1016/j.xjidi.2024.100308_bib13 article-title: affy--analysis of Affymetrix GeneChip data at the probe level publication-title: Bioinformatics doi: 10.1093/bioinformatics/btg405 – volume: 12 start-page: 441 year: 2005 ident: 10.1016/j.xjidi.2024.100308_bib1 article-title: The orphan nuclear receptor RORalpha regulates circadian transcription of the mammalian core-clock Bmal1 publication-title: Nat Struct Mol Biol doi: 10.1038/nsmb925 – volume: 115 start-page: 12313 year: 2018 ident: 10.1016/j.xjidi.2024.100308_bib30 article-title: Population-level rhythms in human skin with implications for circadian medicine publication-title: Proc Natl Acad Sci USA doi: 10.1073/pnas.1809442115 – volume: 8 start-page: 23978 year: 2017 ident: 10.1016/j.xjidi.2024.100308_bib4 article-title: Genetic variation of clock genes and cancer risk: a field synopsis and meta-analysis publication-title: Oncotarget doi: 10.18632/oncotarget.15074 – volume: 22 start-page: 1 year: 2021 ident: 10.1016/j.xjidi.2024.100308_bib19 article-title: The impact of the circadian clock on skin physiology and cancer development publication-title: Int J Mol Sci doi: 10.3390/ijms22116112 – volume: 35 start-page: 445 year: 2012 ident: 10.1016/j.xjidi.2024.100308_bib20 article-title: Central and peripheral circadian clocks in mammals publication-title: Annu Rev Neurosci doi: 10.1146/annurev-neuro-060909-153128 – volume: 595 start-page: 2413 year: 2021 ident: 10.1016/j.xjidi.2024.100308_bib11 article-title: The circadian clock and diseases of the skin publication-title: FEBS Lett doi: 10.1002/1873-3468.14192 – volume: 23 start-page: 602 year: 2016 ident: 10.1016/j.xjidi.2024.100308_bib18 article-title: Brown adipose tissue exhibits a glucose-responsive thermogenic biorhythm in humans publication-title: Cell Metab doi: 10.1016/j.cmet.2016.02.007 – volume: 58 start-page: 231 year: 2018 ident: 10.1016/j.xjidi.2024.100308_bib7 article-title: Development and therapeutic potential of small-molecule modulators of circadian systems publication-title: Annu Rev Pharmacol Toxicol doi: 10.1146/annurev-pharmtox-010617-052645 – volume: 48 start-page: 10 year: 2020 ident: 10.1016/j.xjidi.2024.100308_bib12 article-title: The ex vivo skin model as an alternative tool for the efficacy and safety evaluation of topical products publication-title: Altern Lab Anim doi: 10.1177/0261192920914193 – volume: 21 start-page: 1 year: 2020 ident: 10.1016/j.xjidi.2024.100308_bib5 article-title: Age-induced differential changes in the central and colonic human circadian oscillators publication-title: Int J Mol Sci doi: 10.3390/ijms21020674 – volume: 30 start-page: 163 year: 2015 ident: 10.1016/j.xjidi.2024.100308_bib21 article-title: The circadian clock in skin: implications for adult stem cells, tissue regeneration, cancer, aging, and immunity publication-title: J Biol Rhythms doi: 10.1177/0748730414563537 |
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SubjectTerms | Circadian clock Dermatology Gene expression Original Skin explant UVR |
Title | Analysis of Circadian Clock Gene Expression in Human Skin Explants |
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