紫心马铃薯品种黑美人组织培养技术

[目的]研究紫心马铃薯品种黑美人的组织培养技术,为其种薯工厂化生产提供技术支持.[方法]以紫色马铃薯品种黑美人的顶芽及不同部位腋芽为外植体,探讨0.1％ HgCl2对外植体灭菌、6-BA对初代培养、6-BA和PP333组合对增殖培养、NAA对生根、糖对结薯等过程的影响.[结果]在4.0～9.0min范围内,以0.1％升汞溶液对顶芽、第2段腋芽、第3段腋芽及以下芽分别处理5、6、8 min的灭菌效果最好.在MS培养基中添加0～3.0 mg/L 6-BA,顶芽、第2段腋芽、第3段腋芽及以下芽的适宜初代培养基分别为MS＋1.0 mg/L 6-BA、MS＋1.5 mg/L 6-BA、MS＋2.0 mg...

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Bibliographic Details
Published in南方农业学报 Vol. 45; no. 4; pp. 527 - 531
Main Author 周珊 农艳丰 段维兴 杨美纯
Format Journal Article
LanguageChinese
Published 广西农业科学院甘蔗研究所/中国农业科学院甘蔗研究中心/广西甘蔗遗传改良重点实验室,南宁530007 2014
广西大学农学院,南宁530005%广西大学农学院,南宁,530005%广西农业科学院甘蔗研究所/中国农业科学院甘蔗研究中心/广西甘蔗遗传改良重点实验室,南宁,530007
Subjects
紫心马铃薯
组织培养
试管薯诱导
黑美人
micro-tuber induction
组织培养
紫心马铃薯
tissue culture
试管薯诱导
purple-heart potato
Black Beauty
黑美人
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ISSN2095-1191
DOI10.3969/j:issn.2095-1191.2014.4.527

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Summary:[目的]研究紫心马铃薯品种黑美人的组织培养技术,为其种薯工厂化生产提供技术支持.[方法]以紫色马铃薯品种黑美人的顶芽及不同部位腋芽为外植体,探讨0.1％ HgCl2对外植体灭菌、6-BA对初代培养、6-BA和PP333组合对增殖培养、NAA对生根、糖对结薯等过程的影响.[结果]在4.0～9.0min范围内,以0.1％升汞溶液对顶芽、第2段腋芽、第3段腋芽及以下芽分别处理5、6、8 min的灭菌效果最好.在MS培养基中添加0～3.0 mg/L 6-BA,顶芽、第2段腋芽、第3段腋芽及以下芽的适宜初代培养基分别为MS＋1.0 mg/L 6-BA、MS＋1.5 mg/L 6-BA、MS＋2.0 mg/L6-BA.在MS培养基中添加0～3.0mg/L 6-BA和0～0.4 mg/L PP333,较适宜的增殖培养基为MS＋3.0 mg/L 6-BA＋0.2 mg/L PP333,最高增殖倍数为7.0.在0～0.6mg/L NAA内,较适宜的生根培养基为MS＋0.4 mg/L NAA,最高生根率达100.0％.MS培养基中添加30.0～80.0 mg/L糖,较适宜的结薯培养基为MS＋60.0 g/L糖,最高结薯率达93.3％.[结论]成功构建了紫色马铃薯黑美人的再生繁殖体系,获得无菌苗及试管薯,该繁殖技术可用于其种薯工厂化生产.
Bibliography:ZHOU Shan NONG Yan-feng, DUAN Wei-xing, YANG Mei-chun （1Sugarcane Research Institute,Guangxi Academy of Agricuhural Sciences/Sugarcane Research Center, Chinese Academy of Agricultural Sciences/Guangxi Key Laboratory of Sugarcane Biotechnology and Genetic Improvement, Ministry of Agricultural, Nanning 530007, China; 2Agricultural College, Guangxi University, Nanning 530005, China）
45-1381/S
purple-heart potato ; Black Beauty ; tissue culture ; micro-tuber induction
[Objective]By using different types of explants, tissue culture of purple-heart potato variety Black Beauty was studied in order to provide technical supports for its factory production in Guangxi. [ Method ]The termi- nal buds, the different section axillary buds of potato variety Black Beauty were used as different explants, and MS was used as base medium. The effects of 0.1% HgCl2（Mercuric chloride ） sterilizing time on explants were investigated, as well as 6-BA on primary culture, different concentrations of 6-BA combined with PP333 on proliferati
ISSN:2095-1191
DOI:10.3969/j:issn.2095-1191.2014.4.527