Search for Microorganisms in Men with Urologic Chronic Pelvic Pain Syndrome: A Culture-Independent Analysis in the MAPP Research Network
Purpose We used next-generation, state-of-the-art, culture independent methodology to survey urine microbiota of males with urologic chronic pelvic pain syndrome and control participants enrolled in the MAPP Network to investigate a possible microbial etiology. Materials and Methods Male patients wi...
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| Published in | The Journal of urology Vol. 194; no. 1; pp. 127 - 135 |
|---|---|
| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Elsevier Inc
01.07.2015
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0022-5347 1527-3792 1527-3792 |
| DOI | 10.1016/j.juro.2015.01.037 |
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| Abstract | Purpose We used next-generation, state-of-the-art, culture independent methodology to survey urine microbiota of males with urologic chronic pelvic pain syndrome and control participants enrolled in the MAPP Network to investigate a possible microbial etiology. Materials and Methods Male patients with urologic chronic pelvic pain syndrome and matched controls were asked to provide initial, midstream and post-prostatic massage urine specimens. Specimens were analyzed with Ibis T-5000 Universal Biosensor technology to provide comprehensive identification of bacterial and select fungal species. Differences between urologic chronic pelvic pain syndrome and control study participants for the presence of species or species variation in a higher taxonomic grouping (genus) were evaluated using permutational multivariate analysis of variance and logistic regression. Results Initial and midstream urine specimens were obtained from 110 (post-prostatic massage urine in 67) participants with urologic chronic pelvic pain syndrome and 115 (post-prostatic massage urine in 62) controls. Overall 78, 73 and 54 species (42, 39 and 27 genera) were detected in initial, midstream and post-prostatic massage urine specimens, respectively. Mean (SD) initial, midstream and post-prostatic massage urine species count per person was 1.62 (1.28), 1.38 (1.36) and 1.33 (1.24) for cases, and 1.75 (1.32), 1.23 (1.15) and 1.56 (0.97) for controls, respectively. Overall species and genus composition differed significantly between participants with urologic chronic pelvic pain syndrome and controls in initial stream urine (p=0.002 species level, p=0.004 genus level), with Burkholderia cenocepacia overrepresented in urologic chronic pelvic pain syndrome. No significant differences were observed at any level in midstream or post-prostatic massage urine samples. Conclusions Assessment of baseline culture-independent microbiological data from male subjects enrolled in the MAPP Network has identified overrepresentation of B. cenocepacia in urologic chronic pelvic pain syndrome. Future studies are planned to further evaluate microbiota associations with variable and changing urologic chronic pelvic pain syndrome symptom patterns. |
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| AbstractList | Purpose We used next-generation, state-of-the-art, culture independent methodology to survey urine microbiota of males with urologic chronic pelvic pain syndrome and control participants enrolled in the MAPP Network to investigate a possible microbial etiology. Materials and Methods Male patients with urologic chronic pelvic pain syndrome and matched controls were asked to provide initial, midstream and post-prostatic massage urine specimens. Specimens were analyzed with Ibis T-5000 Universal Biosensor technology to provide comprehensive identification of bacterial and select fungal species. Differences between urologic chronic pelvic pain syndrome and control study participants for the presence of species or species variation in a higher taxonomic grouping (genus) were evaluated using permutational multivariate analysis of variance and logistic regression. Results Initial and midstream urine specimens were obtained from 110 (post-prostatic massage urine in 67) participants with urologic chronic pelvic pain syndrome and 115 (post-prostatic massage urine in 62) controls. Overall 78, 73 and 54 species (42, 39 and 27 genera) were detected in initial, midstream and post-prostatic massage urine specimens, respectively. Mean (SD) initial, midstream and post-prostatic massage urine species count per person was 1.62 (1.28), 1.38 (1.36) and 1.33 (1.24) for cases, and 1.75 (1.32), 1.23 (1.15) and 1.56 (0.97) for controls, respectively. Overall species and genus composition differed significantly between participants with urologic chronic pelvic pain syndrome and controls in initial stream urine (p=0.002 species level, p=0.004 genus level), with Burkholderia cenocepacia overrepresented in urologic chronic pelvic pain syndrome. No significant differences were observed at any level in midstream or post-prostatic massage urine samples. Conclusions Assessment of baseline culture-independent microbiological data from male subjects enrolled in the MAPP Network has identified overrepresentation of B. cenocepacia in urologic chronic pelvic pain syndrome. Future studies are planned to further evaluate microbiota associations with variable and changing urologic chronic pelvic pain syndrome symptom patterns. We used next-generation, state-of-the-art, culture independent methodology to survey urine microbiota of males with urologic chronic pelvic pain syndrome and control participants enrolled in the MAPP Network to investigate a possible microbial etiology. Male patients with urologic chronic pelvic pain syndrome and matched controls were asked to provide initial, midstream and post-prostatic massage urine specimens. Specimens were analyzed with Ibis T-5000 Universal Biosensor technology to provide comprehensive identification of bacterial and select fungal species. Differences between urologic chronic pelvic pain syndrome and control study participants for the presence of species or species variation in a higher taxonomic grouping (genus) were evaluated using permutational multivariate analysis of variance and logistic regression. Initial and midstream urine specimens were obtained from 110 (post-prostatic massage urine in 67) participants with urologic chronic pelvic pain syndrome and 115 (post-prostatic massage urine in 62) controls. Overall 78, 73 and 54 species (42, 39 and 27 genera) were detected in initial, midstream and post-prostatic massage urine specimens, respectively. Mean (SD) initial, midstream and post-prostatic massage urine species count per person was 1.62 (1.28), 1.38 (1.36) and 1.33 (1.24) for cases, and 1.75 (1.32), 1.23 (1.15) and 1.56 (0.97) for controls, respectively. Overall species and genus composition differed significantly between participants with urologic chronic pelvic pain syndrome and controls in initial stream urine (p=0.002 species level, p=0.004 genus level), with Burkholderia cenocepacia overrepresented in urologic chronic pelvic pain syndrome. No significant differences were observed at any level in midstream or post-prostatic massage urine samples. Assessment of baseline culture-independent microbiological data from male subjects enrolled in the MAPP Network has identified overrepresentation of B. cenocepacia in urologic chronic pelvic pain syndrome. Future studies are planned to further evaluate microbiota associations with variable and changing urologic chronic pelvic pain syndrome symptom patterns. We used next-generation, state-of-the-art, culture independent methodology to survey urine microbiota of males with urologic chronic pelvic pain syndrome and control participants enrolled in the MAPP Network to investigate a possible microbial etiology. Male patients with urologic chronic pelvic pain syndrome and matched controls were asked to provide initial, midstream and post-prostatic massage urine specimens. Specimens were analyzed with Ibis T-5000 Universal Biosensor technology to provide comprehensive identification of bacterial and select fungal species. Differences between urologic chronic pelvic pain syndrome and control study participants for the presence of species or species variation in a higher taxonomic grouping (genus) were evaluated using permutational multivariate analysis of variance and logistic regression. Initial and midstream urine specimens were obtained from 110 (post-prostatic massage urine in 67) participants with urologic chronic pelvic pain syndrome and 115 (post-prostatic massage urine in 62) controls. Overall 78, 73 and 54 species (42, 39 and 27 genera) were detected in initial, midstream and post-prostatic massage urine specimens, respectively. Mean (SD) initial, midstream and post-prostatic massage urine species count per person was 1.62 (1.28), 1.38 (1.36) and 1.33 (1.24) for cases, and 1.75 (1.32), 1.23 (1.15) and 1.56 (0.97) for controls, respectively. Overall species and genus composition differed significantly between participants with urologic chronic pelvic pain syndrome and controls in initial stream urine (p=0.002 species level, p=0.004 genus level), with Burkholderia cenocepacia overrepresented in urologic chronic pelvic pain syndrome. No significant differences were observed at any level in midstream or post-prostatic massage urine samples. Assessment of baseline culture-independent microbiological data from male subjects enrolled in the MAPP Network has identified overrepresentation of B. cenocepacia in urologic chronic pelvic pain syndrome. Future studies are planned to further evaluate microbiota associations with variable and changing urologic chronic pelvic pain syndrome symptom patterns. We used next-generation, state-of-the-art, culture independent methodology to survey urine microbiota of males with urologic chronic pelvic pain syndrome and control participants enrolled in the MAPP Network to investigate a possible microbial etiology.PURPOSEWe used next-generation, state-of-the-art, culture independent methodology to survey urine microbiota of males with urologic chronic pelvic pain syndrome and control participants enrolled in the MAPP Network to investigate a possible microbial etiology.Male patients with urologic chronic pelvic pain syndrome and matched controls were asked to provide initial, midstream and post-prostatic massage urine specimens. Specimens were analyzed with Ibis T-5000 Universal Biosensor technology to provide comprehensive identification of bacterial and select fungal species. Differences between urologic chronic pelvic pain syndrome and control study participants for the presence of species or species variation in a higher taxonomic grouping (genus) were evaluated using permutational multivariate analysis of variance and logistic regression.MATERIALS AND METHODSMale patients with urologic chronic pelvic pain syndrome and matched controls were asked to provide initial, midstream and post-prostatic massage urine specimens. Specimens were analyzed with Ibis T-5000 Universal Biosensor technology to provide comprehensive identification of bacterial and select fungal species. Differences between urologic chronic pelvic pain syndrome and control study participants for the presence of species or species variation in a higher taxonomic grouping (genus) were evaluated using permutational multivariate analysis of variance and logistic regression.Initial and midstream urine specimens were obtained from 110 (post-prostatic massage urine in 67) participants with urologic chronic pelvic pain syndrome and 115 (post-prostatic massage urine in 62) controls. Overall 78, 73 and 54 species (42, 39 and 27 genera) were detected in initial, midstream and post-prostatic massage urine specimens, respectively. Mean (SD) initial, midstream and post-prostatic massage urine species count per person was 1.62 (1.28), 1.38 (1.36) and 1.33 (1.24) for cases, and 1.75 (1.32), 1.23 (1.15) and 1.56 (0.97) for controls, respectively. Overall species and genus composition differed significantly between participants with urologic chronic pelvic pain syndrome and controls in initial stream urine (p=0.002 species level, p=0.004 genus level), with Burkholderia cenocepacia overrepresented in urologic chronic pelvic pain syndrome. No significant differences were observed at any level in midstream or post-prostatic massage urine samples.RESULTSInitial and midstream urine specimens were obtained from 110 (post-prostatic massage urine in 67) participants with urologic chronic pelvic pain syndrome and 115 (post-prostatic massage urine in 62) controls. Overall 78, 73 and 54 species (42, 39 and 27 genera) were detected in initial, midstream and post-prostatic massage urine specimens, respectively. Mean (SD) initial, midstream and post-prostatic massage urine species count per person was 1.62 (1.28), 1.38 (1.36) and 1.33 (1.24) for cases, and 1.75 (1.32), 1.23 (1.15) and 1.56 (0.97) for controls, respectively. Overall species and genus composition differed significantly between participants with urologic chronic pelvic pain syndrome and controls in initial stream urine (p=0.002 species level, p=0.004 genus level), with Burkholderia cenocepacia overrepresented in urologic chronic pelvic pain syndrome. No significant differences were observed at any level in midstream or post-prostatic massage urine samples.Assessment of baseline culture-independent microbiological data from male subjects enrolled in the MAPP Network has identified overrepresentation of B. cenocepacia in urologic chronic pelvic pain syndrome. Future studies are planned to further evaluate microbiota associations with variable and changing urologic chronic pelvic pain syndrome symptom patterns.CONCLUSIONSAssessment of baseline culture-independent microbiological data from male subjects enrolled in the MAPP Network has identified overrepresentation of B. cenocepacia in urologic chronic pelvic pain syndrome. Future studies are planned to further evaluate microbiota associations with variable and changing urologic chronic pelvic pain syndrome symptom patterns. |
| Author | Ehrlich, Garth D Lucia, M. Scott Chen, Jun van Bokhoven, Adrie Landis, J. Richard Stephens, Alisa Mullins, Chris Melton-Kreft, Rachael Nickel, J. Curtis |
| AuthorAffiliation | 1 Department of Urology, Queen’s University, Kingston, Ontario, Canada 7 Departments of Microbiology & Immunology and Otolaryngology-Head and Neck Surgery, Drexel University College of Medicine, Philadelphia, PA 4 National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 2 Department of Biostatistics and Epidemiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 5 Department of Pathology, University of Colorado Anschutz Medical Campus, Aurora, CO 3 Division of Biomedical Statistics and Informatics, Mayo Clinc, Rochester, MN 6 Allegheny Health Network, Center of Excellence in Biofilm Research, Pittsburgh, PA 8 See Appendix 1 |
| AuthorAffiliation_xml | – name: 1 Department of Urology, Queen’s University, Kingston, Ontario, Canada – name: 2 Department of Biostatistics and Epidemiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA – name: 3 Division of Biomedical Statistics and Informatics, Mayo Clinc, Rochester, MN – name: 5 Department of Pathology, University of Colorado Anschutz Medical Campus, Aurora, CO – name: 7 Departments of Microbiology & Immunology and Otolaryngology-Head and Neck Surgery, Drexel University College of Medicine, Philadelphia, PA – name: 8 See Appendix 1 – name: 4 National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD – name: 6 Allegheny Health Network, Center of Excellence in Biofilm Research, Pittsburgh, PA |
| Author_xml | – sequence: 1 fullname: Nickel, J. Curtis – sequence: 2 fullname: Stephens, Alisa – sequence: 3 fullname: Landis, J. Richard – sequence: 4 fullname: Chen, Jun – sequence: 5 fullname: Mullins, Chris – sequence: 6 fullname: van Bokhoven, Adrie – sequence: 7 fullname: Lucia, M. Scott – sequence: 8 fullname: Melton-Kreft, Rachael – sequence: 9 fullname: Ehrlich, Garth D |
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| Cites_doi | 10.1128/JCM.34.12.3120-3128.1996 10.1128/JCM.36.6.1646-1652.1998 10.1016/S0022-5347(05)67987-6 10.1126/science.284.5418.1318 10.1186/1471-2490-14-58 10.1097/BOT.0000000000000043 10.1016/S0090-4295(98)00553-6 10.1038/nrmicro1918 10.1128/IAI.00910-10 10.1186/1471-2490-14-57 10.5489/cuaj.857 10.1038/ncpuro0713 10.1096/fj.12-220806 10.1097/01.ju.0000082252.49374.e9 10.1002/pros.10218 10.2106/JBJS.L.00210 10.1016/S0022-5347(17)37247-6 10.1128/IAI.00768-12 10.1128/JCM.37.6.1863-1870.1999 10.1002/pros.2990230204 10.1111/j.2517-6161.1995.tb02031.x 10.1371/journal.pone.0060987 |
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| References | Tanner, Shoskes, Shahed (bib7) 1999; 37 Shoskes, Shahed (bib8) 2000; 6 Ecker, Sampath, Massire (bib17) 2008; 6 Costerton, Stewart, Greenberg (bib2) 1999; 284 Ehrlich, Greenberg (bib6) 1994 Benjamini, Hochberg (bib18) 1995; 57 Hochreiter, Duncan, Schaeffer (bib12) 2000; 163 Xie, Huang, Yang (bib14) 2006; 86 Organ, Grantmyre, Hutchinson (bib24) 2010; 4 Landis, Williams, Lucia (bib15) 2014; 14 Rudick, Berry, Johnson (bib26) 2011; 79 Nickel, Costerton (bib4) 1992; 147 Keay, Zhang, Baldwin (bib11) 1999; 53 Nickel (bib1) 1999 Schwager, Agnoli, Köthe (bib22) 2013; 81 Quick, Wong, Mukherjee (bib27) 2013; 8 Leskinen, Rantakokko-Jalava, Manninen (bib13) 2003; 55 Wolcott, Ehrlich (bib3) 2008; 11 Nickel, Costerton (bib5) 1993; 23 Krieger, Riley, Roberts (bib9) 1996; 34 Jacovides, Kreft, Adeli (bib20) 2012; 94 Aagaard, Petrosino, Keitel (bib28) 2013; 27 Arzola, Hawley, Oakman (bib23) 2007; 4 Nickel, Alexander, Schaeffer (bib25) 2003; 170 Anderson (bib19) 2001; 26 Riley, Berger, Miner (bib10) 1998; 36 Clemens, Mullins, Kusek (bib16) 2014; 14 Palmer, Altman, Alman (bib21) 2014; 28 Riley (10.1016/j.juro.2015.01.037_bib10) 1998; 36 Quick (10.1016/j.juro.2015.01.037_bib27) 2013; 8 Tanner (10.1016/j.juro.2015.01.037_bib7) 1999; 37 Jacovides (10.1016/j.juro.2015.01.037_bib20) 2012; 94 Krieger (10.1016/j.juro.2015.01.037_bib9) 1996; 34 Nickel (10.1016/j.juro.2015.01.037_bib5) 1993; 23 Aagaard (10.1016/j.juro.2015.01.037_bib28) 2013; 27 Nickel (10.1016/j.juro.2015.01.037_bib25) 2003; 170 Benjamini (10.1016/j.juro.2015.01.037_bib18) 1995; 57 Costerton (10.1016/j.juro.2015.01.037_bib2) 1999; 284 Xie (10.1016/j.juro.2015.01.037_bib14) 2006; 86 Wolcott (10.1016/j.juro.2015.01.037_bib3) 2008; 11 Hochreiter (10.1016/j.juro.2015.01.037_bib12) 2000; 163 Landis (10.1016/j.juro.2015.01.037_bib15) 2014; 14 Nickel (10.1016/j.juro.2015.01.037_bib1) 1999 Keay (10.1016/j.juro.2015.01.037_bib11) 1999; 53 Ehrlich (10.1016/j.juro.2015.01.037_bib6) 1994 Shoskes (10.1016/j.juro.2015.01.037_bib8) 2000; 6 Clemens (10.1016/j.juro.2015.01.037_bib16) 2014; 14 Anderson (10.1016/j.juro.2015.01.037_bib19) 2001; 26 Schwager (10.1016/j.juro.2015.01.037_bib22) 2013; 81 Rudick (10.1016/j.juro.2015.01.037_bib26) 2011; 79 Nickel (10.1016/j.juro.2015.01.037_bib4) 1992; 147 Arzola (10.1016/j.juro.2015.01.037_bib23) 2007; 4 Leskinen (10.1016/j.juro.2015.01.037_bib13) 2003; 55 Ecker (10.1016/j.juro.2015.01.037_bib17) 2008; 6 Palmer (10.1016/j.juro.2015.01.037_bib21) 2014; 28 Organ (10.1016/j.juro.2015.01.037_bib24) 2010; 4 25892141 - J Urol. 2015 Jul;194(1):14-5. doi: 10.1016/j.juro.2015.04.070. |
| References_xml | – start-page: 3 year: 1999 ident: bib1 article-title: Prostatitis: an historical perspective publication-title: Textbook of Prostatitis – volume: 94 start-page: 2247 year: 2012 ident: bib20 article-title: Successful identification of pathogens by polymerase chain reaction (PCR)-based electron spray ionization time-of-flight mass spectrometry (ESI-TOF-MS) in culture-negative periprosthetic joint infection publication-title: J Bone Joint Surg Am – volume: 86 start-page: 976 year: 2006 ident: bib14 article-title: Detection of 16s ribosomal RNA gene of bacteria in prostate tissues of adults publication-title: Zhonghua Yi Xue Za Zhi – volume: 55 start-page: 105 year: 2003 ident: bib13 article-title: Negative bacterial polymerase chain reaction (PCR) findings in prostate tissue from patients with symptoms of chronic pelvic pain syndrome (CPPS) and localized prostate cancer publication-title: Prostate – volume: 79 start-page: 628 year: 2011 ident: bib26 article-title: Uropathogenic Escherichia coli induces chronic pelvic pain publication-title: Infect Immun – volume: 23 start-page: 107 year: 1993 ident: bib5 article-title: Bacterial localization in antibiotic-refractory chronic bacterial prostatitis publication-title: Prostate – volume: 147 start-page: 398 year: 1992 ident: bib4 article-title: Coagulase-negative staphylococcus in chronic prostatitis publication-title: J Urol – volume: 163 start-page: 127 year: 2000 ident: bib12 article-title: Evaluation of the bacterial flora of the prostate using a rRNA gene based polymerase chain reaction publication-title: J Urol – volume: 26 start-page: 32 year: 2001 ident: bib19 article-title: A new method for non-parametric multivariate analysis of variance publication-title: Austral Ecol – volume: 34 start-page: 3120 year: 1996 ident: bib9 article-title: Prokaryotic DNA sequences in patients with chronic idiopathic prostatitis publication-title: J Clin Microbiol – volume: 14 start-page: 57 year: 2014 ident: bib16 article-title: The MAPP Research Network: a novel study of urologic chronic pelvic pain syndromes publication-title: BMC Urol – volume: 57 start-page: 289 year: 1995 ident: bib18 article-title: Controlling the false discovery rate: a practical and powerful approach to multiple testing publication-title: J R Stat Soc B – year: 1994 ident: bib6 article-title: PCR-Based Diagnostics in Infectious Disease – volume: 27 start-page: 1012 year: 2013 ident: bib28 article-title: The human microbiome project strategy for comprehensive sampling of the human microbiome and why it matters publication-title: J FASEB – volume: 36 start-page: 1646 year: 1998 ident: bib10 article-title: Diverse and related 16S rRNA-encoding DNA sequences in prostate tissues of men with chronic prostatitis publication-title: J Clin Microbiol – volume: 6 start-page: 240 year: 2000 ident: bib8 article-title: Detection of bacterial signal by 16S rRNA polymerase chain reaction in expressed prostatic secretions predicts response to antibiotic therapy in men with chronic pelvic pain syndrome publication-title: Tech Urol – volume: 81 start-page: 143 year: 2013 ident: bib22 article-title: Identification of Burkholderia cenocepacia strain H111 virulence factors using nonmammalian infection hosts publication-title: Infect Immun – volume: 6 start-page: 553 year: 2008 ident: bib17 article-title: Ibis T5000: a universal biosensor approach for microbiology publication-title: Nat Rev Microbiol – volume: 284 start-page: 1318 year: 1999 ident: bib2 article-title: Bacterial biofilms: a common cause of persistent infections publication-title: Science – volume: 4 start-page: 111 year: 2007 ident: bib23 article-title: A case of prostatitis due to Burkholderia pseudomallei publication-title: Nat Clin Pract Urol – volume: 11 start-page: 299 year: 2008 ident: bib3 article-title: Biofilms and chronic infections publication-title: JAMA – volume: 28 start-page: 384 year: 2014 ident: bib21 article-title: Can we trust intraoperative culture results in nonunions? publication-title: J Orthop Trauma – volume: 37 start-page: 1863 year: 1999 ident: bib7 article-title: Prevalence of corynebacterial 16S rRNA sequences in patients with bacterial and “nonbacterial” prostatitis publication-title: J Clin Microbiol – volume: 4 start-page: E58 year: 2010 ident: bib24 article-title: Burkholderia cepacia infection of the prostate caused by inoculation of contaminated ultrasound gel during transrectal biopsy of the prostate publication-title: Can Urol Assoc J – volume: 53 start-page: 487 year: 1999 ident: bib11 article-title: Polymerase chain reaction amplification of bacterial 16s rRNA genes in prostate biopsies from men without chronic prostatitis publication-title: Urology – volume: 14 start-page: 58 year: 2014 ident: bib15 article-title: The MAPP Research Network: design, patient characterization and operations publication-title: BMC Urol – volume: 170 start-page: 818 year: 2003 ident: bib25 article-title: Leukocytes and bacteria in men with chronic prostatitis/chronic pelvic pain syndrome compared to asymptomatic controls publication-title: J Urol – volume: 8 start-page: e60987 year: 2013 ident: bib27 article-title: Th1-Th17 cells contribute to the development of uropathogenic Escherichia coli-induced chronic pelvic pain publication-title: PLoS One – volume: 34 start-page: 3120 year: 1996 ident: 10.1016/j.juro.2015.01.037_bib9 article-title: Prokaryotic DNA sequences in patients with chronic idiopathic prostatitis publication-title: J Clin Microbiol doi: 10.1128/JCM.34.12.3120-3128.1996 – volume: 36 start-page: 1646 year: 1998 ident: 10.1016/j.juro.2015.01.037_bib10 article-title: Diverse and related 16S rRNA-encoding DNA sequences in prostate tissues of men with chronic prostatitis publication-title: J Clin Microbiol doi: 10.1128/JCM.36.6.1646-1652.1998 – volume: 163 start-page: 127 year: 2000 ident: 10.1016/j.juro.2015.01.037_bib12 article-title: Evaluation of the bacterial flora of the prostate using a rRNA gene based polymerase chain reaction publication-title: J Urol doi: 10.1016/S0022-5347(05)67987-6 – volume: 284 start-page: 1318 year: 1999 ident: 10.1016/j.juro.2015.01.037_bib2 article-title: Bacterial biofilms: a common cause of persistent infections publication-title: Science doi: 10.1126/science.284.5418.1318 – volume: 14 start-page: 58 year: 2014 ident: 10.1016/j.juro.2015.01.037_bib15 article-title: The MAPP Research Network: design, patient characterization and operations publication-title: BMC Urol doi: 10.1186/1471-2490-14-58 – volume: 28 start-page: 384 year: 2014 ident: 10.1016/j.juro.2015.01.037_bib21 article-title: Can we trust intraoperative culture results in nonunions? publication-title: J Orthop Trauma doi: 10.1097/BOT.0000000000000043 – volume: 53 start-page: 487 year: 1999 ident: 10.1016/j.juro.2015.01.037_bib11 article-title: Polymerase chain reaction amplification of bacterial 16s rRNA genes in prostate biopsies from men without chronic prostatitis publication-title: Urology doi: 10.1016/S0090-4295(98)00553-6 – year: 1994 ident: 10.1016/j.juro.2015.01.037_bib6 – volume: 86 start-page: 976 year: 2006 ident: 10.1016/j.juro.2015.01.037_bib14 article-title: Detection of 16s ribosomal RNA gene of bacteria in prostate tissues of adults publication-title: Zhonghua Yi Xue Za Zhi – volume: 6 start-page: 553 year: 2008 ident: 10.1016/j.juro.2015.01.037_bib17 article-title: Ibis T5000: a universal biosensor approach for microbiology publication-title: Nat Rev Microbiol doi: 10.1038/nrmicro1918 – volume: 79 start-page: 628 year: 2011 ident: 10.1016/j.juro.2015.01.037_bib26 article-title: Uropathogenic Escherichia coli induces chronic pelvic pain publication-title: Infect Immun doi: 10.1128/IAI.00910-10 – volume: 6 start-page: 240 year: 2000 ident: 10.1016/j.juro.2015.01.037_bib8 article-title: Detection of bacterial signal by 16S rRNA polymerase chain reaction in expressed prostatic secretions predicts response to antibiotic therapy in men with chronic pelvic pain syndrome publication-title: Tech Urol – volume: 14 start-page: 57 year: 2014 ident: 10.1016/j.juro.2015.01.037_bib16 article-title: The MAPP Research Network: a novel study of urologic chronic pelvic pain syndromes publication-title: BMC Urol doi: 10.1186/1471-2490-14-57 – volume: 4 start-page: E58 year: 2010 ident: 10.1016/j.juro.2015.01.037_bib24 article-title: Burkholderia cepacia infection of the prostate caused by inoculation of contaminated ultrasound gel during transrectal biopsy of the prostate publication-title: Can Urol Assoc J doi: 10.5489/cuaj.857 – volume: 11 start-page: 299 year: 2008 ident: 10.1016/j.juro.2015.01.037_bib3 article-title: Biofilms and chronic infections publication-title: JAMA – volume: 4 start-page: 111 year: 2007 ident: 10.1016/j.juro.2015.01.037_bib23 article-title: A case of prostatitis due to Burkholderia pseudomallei publication-title: Nat Clin Pract Urol doi: 10.1038/ncpuro0713 – start-page: 3 year: 1999 ident: 10.1016/j.juro.2015.01.037_bib1 article-title: Prostatitis: an historical perspective – volume: 27 start-page: 1012 year: 2013 ident: 10.1016/j.juro.2015.01.037_bib28 article-title: The human microbiome project strategy for comprehensive sampling of the human microbiome and why it matters publication-title: J FASEB doi: 10.1096/fj.12-220806 – volume: 170 start-page: 818 year: 2003 ident: 10.1016/j.juro.2015.01.037_bib25 article-title: Leukocytes and bacteria in men with chronic prostatitis/chronic pelvic pain syndrome compared to asymptomatic controls publication-title: J Urol doi: 10.1097/01.ju.0000082252.49374.e9 – volume: 55 start-page: 105 year: 2003 ident: 10.1016/j.juro.2015.01.037_bib13 article-title: Negative bacterial polymerase chain reaction (PCR) findings in prostate tissue from patients with symptoms of chronic pelvic pain syndrome (CPPS) and localized prostate cancer publication-title: Prostate doi: 10.1002/pros.10218 – volume: 94 start-page: 2247 year: 2012 ident: 10.1016/j.juro.2015.01.037_bib20 article-title: Successful identification of pathogens by polymerase chain reaction (PCR)-based electron spray ionization time-of-flight mass spectrometry (ESI-TOF-MS) in culture-negative periprosthetic joint infection publication-title: J Bone Joint Surg Am doi: 10.2106/JBJS.L.00210 – volume: 147 start-page: 398 year: 1992 ident: 10.1016/j.juro.2015.01.037_bib4 article-title: Coagulase-negative staphylococcus in chronic prostatitis publication-title: J Urol doi: 10.1016/S0022-5347(17)37247-6 – volume: 26 start-page: 32 year: 2001 ident: 10.1016/j.juro.2015.01.037_bib19 article-title: A new method for non-parametric multivariate analysis of variance publication-title: Austral Ecol – volume: 81 start-page: 143 year: 2013 ident: 10.1016/j.juro.2015.01.037_bib22 article-title: Identification of Burkholderia cenocepacia strain H111 virulence factors using nonmammalian infection hosts publication-title: Infect Immun doi: 10.1128/IAI.00768-12 – volume: 37 start-page: 1863 year: 1999 ident: 10.1016/j.juro.2015.01.037_bib7 article-title: Prevalence of corynebacterial 16S rRNA sequences in patients with bacterial and “nonbacterial” prostatitis publication-title: J Clin Microbiol doi: 10.1128/JCM.37.6.1863-1870.1999 – volume: 23 start-page: 107 year: 1993 ident: 10.1016/j.juro.2015.01.037_bib5 article-title: Bacterial localization in antibiotic-refractory chronic bacterial prostatitis publication-title: Prostate doi: 10.1002/pros.2990230204 – volume: 57 start-page: 289 year: 1995 ident: 10.1016/j.juro.2015.01.037_bib18 article-title: Controlling the false discovery rate: a practical and powerful approach to multiple testing publication-title: J R Stat Soc B doi: 10.1111/j.2517-6161.1995.tb02031.x – volume: 8 start-page: e60987 year: 2013 ident: 10.1016/j.juro.2015.01.037_bib27 article-title: Th1-Th17 cells contribute to the development of uropathogenic Escherichia coli-induced chronic pelvic pain publication-title: PLoS One doi: 10.1371/journal.pone.0060987 – reference: 25892141 - J Urol. 2015 Jul;194(1):14-5. doi: 10.1016/j.juro.2015.04.070. |
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| Snippet | Purpose We used next-generation, state-of-the-art, culture independent methodology to survey urine microbiota of males with urologic chronic pelvic pain... We used next-generation, state-of-the-art, culture independent methodology to survey urine microbiota of males with urologic chronic pelvic pain syndrome and... |
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| SubjectTerms | Bacteria - isolation & purification chronic pain Humans infection Male microbiota pelvic pain prostatitis Prostatitis - microbiology Prostatitis - urine Urinalysis Urology |
| Title | Search for Microorganisms in Men with Urologic Chronic Pelvic Pain Syndrome: A Culture-Independent Analysis in the MAPP Research Network |
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