甘蔗种质资源抗宿根矮化病特性鉴定

【目的】筛选抗甘蔗宿根矮化病（RSD）种质资源材料，为选育抗RSD甘蔗品种提供优良抗原。【方法】采用浸蔗种的方法对6个系列100份甘蔗品种进行侵染，用建立的RSD—PcR检测方法进行检测，计算各甘蔗品种RSD发病率，并对甘蔗种质资源进行抗性评价。【结果】100份甘蔗品种材料中，GT02／351、CP89／2134、ROC26、粤94／28、福农89／209、崖96／45等品种RSD发病率为0，表现为A级抗性；GT99／181、GT97／228、GT90／95等品种RSD发病率等于10％，表现为B级抗性：GT02／208、GT96／156、ROC22等品种表现为C级抗性；GT21、GT02／23...

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Bibliographic Details
Published in南方农业学报 Vol. 43; no. 12; pp. 1963 - 1967
Main Author 张妍 范业赓 莫璋红 吴凯朝 李鸣 秦新民 梁俊
Format Journal Article
LanguageChinese
Published 广西师范大学生命科学学院,广西桂林541600%中国农业科学院甘蔗研究中心/广西农业科学院甘蔗研究所/农业部广西甘蔗生物技术与遗传改良重点实验室/广西甘蔗遗传改良重点实验室,南宁,530007%广西师范大学生命科学学院,广西桂林,541600%广西作物遗传改良生物技术重点开放实验室,南宁530007 2012
中国农业科学院甘蔗研究中心/广西农业科学院甘蔗研究所/农业部广西甘蔗生物技术与遗传改良重点实验室/广西甘蔗遗传改良重点实验室,南宁530007
广西作物遗传改良生物技术重点开放实验室,南宁530007
Subjects
宿根矮化病
抗性评价
甘蔗
种质资源
宿根矮化病
抗性评价
甘蔗
种质资源
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ISSN2095-1191
DOI10.3969/j:issn.2095-1191.2012.12.1963

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Summary:【目的】筛选抗甘蔗宿根矮化病（RSD）种质资源材料，为选育抗RSD甘蔗品种提供优良抗原。【方法】采用浸蔗种的方法对6个系列100份甘蔗品种进行侵染，用建立的RSD—PcR检测方法进行检测，计算各甘蔗品种RSD发病率，并对甘蔗种质资源进行抗性评价。【结果】100份甘蔗品种材料中，GT02／351、CP89／2134、ROC26、粤94／28、福农89／209、崖96／45等品种RSD发病率为0，表现为A级抗性；GT99／181、GT97／228、GT90／95等品种RSD发病率等于10％，表现为B级抗性：GT02／208、GT96／156、ROC22等品种表现为C级抗性；GT21、GT02／237、CP88／1762等品种表现为D级抗性。6个不同系列中，cP系NRSD平均发病率最低，为12．00％，抗性最强；粤糖系列RSD平均发病率最高，为28．57％，抗性较弱。【结论】GT02／351、CP89／2134、ROC26、粤94／28、福农89／209、崖96／45等甘蔗品种具有良好的抗性，具有推广应用价值；CP系列的抗性较强，可作为抗RSD的育种材料。
Bibliography:45-1381/S
[Objective ]This research screened out sugarcane ratoon stunting disease （RSD） germplasm resources in or- der to breed better sugarcane varieties with excellent RSD resistance trait. [Method]By infusing 100 sugarcane varieties from 6 series using the sugarcane seedling-soaking method, the varieties were tested with the established PSD-PCR de- tection method, the RSD incidence rate in the sugarcane varieties were calculated, and the sugarcane germplasm re- sources underwent resistance evaluation. [ Result ] Within the 100 tested sugarcane varieties, the RSD incidence rates of GT02/351, CP89/2134, ROC26, Yue 94/28, Funong 89/209, and Ya 96/45 were 0% and were rated with grade A resis- tance; the RSD incidence rates of GT99/181, GT97/228, and GT90/95 were less than 10% and were rated with grade B resistance. GT02/208, GT96/156, and ROC22 were all rated with grade C resistance, while GT21, GT02/237, and CP88/ 1762 were all rated with grade D resistance. Within the 6 different series, the RSD incidence ra
ISSN:2095-1191
DOI:10.3969/j:issn.2095-1191.2012.12.1963