高灵敏度化学发光磁酶免疫法检测人绒毛膜促性腺激素
将磁性微粒与抗体的偶联，通过优化偶联条件提高了偶联效率，制备了高灵敏度的磁微粒生物探针；采用3-（2-螺旋金刚烷）4-甲氧基-4-（3-邻氧酰苯基）-1，2-二氧杂环丁烷（AMPPD）-碱性磷酸酶（ALP）化学发光体系，建立了化学发光磁酶免疫检测方法；对检测方法进行了优化和改进，提高了系统的灵敏度和检测速度，并对HCG样品进行相关检测。结果表明，HCG浓度在0．15—150 IU／L范围内，光强随HCG浓度增大而增加，两者之间线性关系良好，相关系数r为0．960；检出限可达0．15IU／L；相对标准偏差（RSD）小于5％；检测总时间少于1h。本方法可以应用于其它免疫分子的检测，在临床免疫检测方...
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| Published in | Fēnxī huàxué Vol. 37; no. 7; pp. 985 - 988 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
中国科学院研究生院,北京,100080
2009
中国科学院电子学研究所传感技术联合国家重点实验室,北京,100190 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 0253-3820 |
| DOI | 10.3321/j.issn:0253-3820.2009.07.009 |
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| Abstract | 将磁性微粒与抗体的偶联，通过优化偶联条件提高了偶联效率，制备了高灵敏度的磁微粒生物探针；采用3-（2-螺旋金刚烷）4-甲氧基-4-（3-邻氧酰苯基）-1，2-二氧杂环丁烷（AMPPD）-碱性磷酸酶（ALP）化学发光体系，建立了化学发光磁酶免疫检测方法；对检测方法进行了优化和改进，提高了系统的灵敏度和检测速度，并对HCG样品进行相关检测。结果表明，HCG浓度在0．15—150 IU／L范围内，光强随HCG浓度增大而增加，两者之间线性关系良好，相关系数r为0．960；检出限可达0．15IU／L；相对标准偏差（RSD）小于5％；检测总时间少于1h。本方法可以应用于其它免疫分子的检测，在临床免疫检测方面具有广阔的应用前景。 |
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| AbstractList | 将磁性微粒与抗体的偶联，通过优化偶联条件提高了偶联效率，制备了高灵敏度的磁微粒生物探针；采用3-（2-螺旋金刚烷）4-甲氧基-4-（3-邻氧酰苯基）-1，2-二氧杂环丁烷（AMPPD）-碱性磷酸酶（ALP）化学发光体系，建立了化学发光磁酶免疫检测方法；对检测方法进行了优化和改进，提高了系统的灵敏度和检测速度，并对HCG样品进行相关检测。结果表明，HCG浓度在0．15—150 IU／L范围内，光强随HCG浓度增大而增加，两者之间线性关系良好，相关系数r为0．960；检出限可达0．15IU／L；相对标准偏差（RSD）小于5％；检测总时间少于1h。本方法可以应用于其它免疫分子的检测，在临床免疫检测方面具有广阔的应用前景。 O6; 将磁性微粒与抗体的偶联,通过优化偶联条件提高了偶联效率,制备了高灵敏度的磁微粒生物探针;采用3-(2-螺旋金刚烷)-4-甲氧基-4-(3-邻氧酰苯基)-1,2-二氧杂环丁烷(AMPPD)--碱性磷酸酶(ALP)化学发光体系,建立了化学发光磁酶免疫检测方法;对检测方法进行了优化和改进,提高了系统的灵敏度和检测速度,并对HCG样品进行相关检测.结果表明,HCG浓度在0.15～150 IU/L范围内,光强随HCG浓度增大而增加,两者之间线性关系良好,相关系数r为0.960;检出限可达0.15 IU/L; 相对标准偏差(RSD)小于5%; 检测总时间少于1 h.本方法可以应用于其它免疫分子的检测,在临床免疫检测方面具有广阔的应用前景. |
| Abstract_FL | A highly sensitive magnetic enzymE-linked chemiluminescent immunoassay method was developed for the detection of human chorionic gonadotropin(HCG). The monoclonal antibody was covalently coupled on the surface of carboxylated magnetic beads to generate magnetic-biotargeting; Alkaline phosphatase(ALP) was utilized as a labeled reagent of another monoclonal antibody, whereas 3-(2-spimadamantane) 4-methoxy-4-(3-phosphoryloxy)phenyl-1,2-dioxetane(AMPPD) was utilized as the chemiluminescent substrate. Based on this concept, a highly sensitive chemiluminescent immunoassay method was established to test HCG. Then, several modifications were made to optimize the method, and the detection sensitivity and procedure were improved accordingly. The detection of the assay could be fulfilled within 60 min and the test result of HCG concentration was linear over the range of 0.15 150 IU/L with good relativity(r=0.960). The relative standard deviation(RSD) were below 5% and the sensitivity of this method was 0.15 IU/L. The proposed method with wide linear range, simple operation and fast detection showed good prospect in practical application on-site. |
| Author | 刘军涛 刘儒平 王蜜霞 刘春秀 罗金平 蔡新霞 |
| AuthorAffiliation | 中国科学院电子学研究所传感技术联合国家重点实验室,北京100190 中国科学院研究生院,北京100080 |
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| Author_FL | WANG Mi-Xia LIU Chun-Xiu LUO Jin-Ping LIU Jun-Tao CAI Xin-Xia LIU Ru-Ping |
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| Keywords | Chemiluminescence magnetic immunoassay human chorionic gonadotropin 磁性微球免疫分析 化学发光 人绒毛膜促性腺激素 |
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| Snippet | 将磁性微粒与抗体的偶联，通过优化偶联条件提高了偶联效率，制备了高灵敏度的磁微粒生物探针；采用3-（2-螺旋金刚烷）4-甲氧基-4-（3-邻氧酰苯基）-1，2-二氧杂环丁... O6; 将磁性微粒与抗体的偶联,通过优化偶联条件提高了偶联效率,制备了高灵敏度的磁微粒生物探针;采用3-(2-螺旋金刚烷)-4-甲氧基-4-(3-邻氧酰苯基)-1,2-二氧杂环丁... |
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| Title | 高灵敏度化学发光磁酶免疫法检测人绒毛膜促性腺激素 |
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