木质素增量取食对台湾乳白蚁消化系统的影响
木质素代谢是白蚁消化木质纤维素的关键环节之一。本文利用木质素增量喂食台湾乳白蚁Coptotermes formosanus Shiraki,通过测定其取食量、体内酚氧化酶含量、纤维素酶活性的变化及后肠微生物群落16s rRNA基因Illumina Hi Seq高通量测序比较分析来研究木质素取食对低等白蚁消化系统的影响。结果表明：木质素取食组内源性（前肠/唾液腺）酚氧化酶含量的显著大于大于对照组,内源及外源性纤维素酶活性均小于对照组,尤其BG酶活性显著小于对照组;1%木质素对乳白蚁取食有一定的抑制作用,其后肠细菌多样性及丰度较对照组有所下降,但无显著的组间群落结构差异;Metastat、LDA（...
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| Published in | 环境昆虫学报 Vol. 39; no. 3; pp. 632 - 639 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
广东省生物资源应用研究所,广东省动物保护与资源利用重点实验室,广东省野生动物保护与利用公共实验室,广州510260
2017
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1674-0858 |
| DOI | 10.3969/j.issn.1674-0858.2017.03.18 |
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| Abstract | 木质素代谢是白蚁消化木质纤维素的关键环节之一。本文利用木质素增量喂食台湾乳白蚁Coptotermes formosanus Shiraki,通过测定其取食量、体内酚氧化酶含量、纤维素酶活性的变化及后肠微生物群落16s rRNA基因Illumina Hi Seq高通量测序比较分析来研究木质素取食对低等白蚁消化系统的影响。结果表明：木质素取食组内源性（前肠/唾液腺）酚氧化酶含量的显著大于大于对照组,内源及外源性纤维素酶活性均小于对照组,尤其BG酶活性显著小于对照组;1%木质素对乳白蚁取食有一定的抑制作用,其后肠细菌多样性及丰度较对照组有所下降,但无显著的组间群落结构差异;Metastat、LDA（LDA Effect Size）及组间T-检验属水平群落差异分析显示,乳白蚁后肠核心菌群（相对丰度〉1%）Dysgonomonas属、弧菌属在木质素取食组中相对丰度显著小于对照组,Candidatus Azobacteriodes属相对丰度则显著大于对照组,它们在木质素增量取食引起的细菌群落变化中起关键作用。 |
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| AbstractList | Q965%S433; 木质素代谢是白蚁消化木质纤维素的关键环节之一.本文利用木质素增量喂食台湾乳白蚁Coptotermes formosanus Shiraki,通过测定其取食量、体内酚氧化酶含量、纤维素酶活性的变化及后肠微生物群落16s rRNA基因Illumina HiSeq高通量测序比较分析来研究木质素取食对低等白蚁消化系统的影响.结果表明:木质素取食组内源性(前肠/唾液腺)酚氧化酶含量的显著大于大于对照组,内源及外源性纤维素酶活性均小于对照组,尤其BG酶活性显著小于对照组;1％木质素对乳白蚁取食有一定的抑制作用,其后肠细菌多样性及丰度较对照组有所下降,但无显著的组间群落结构差异;Metastat、LDA (LDA Effect Size)及组间T-检验属水平群落差异分析显示,乳白蚁后肠核心菌群(相对丰度＞1％)Dysgonomonas属、弧菌属在木质素取食组中相对丰度显著小于对照组,Candidatus Azobacteriodes属相对丰度则显著大于对照组,它们在木质素增量取食引起的细菌群落变化中起关键作用. 木质素代谢是白蚁消化木质纤维素的关键环节之一。本文利用木质素增量喂食台湾乳白蚁Coptotermes formosanus Shiraki,通过测定其取食量、体内酚氧化酶含量、纤维素酶活性的变化及后肠微生物群落16s rRNA基因Illumina Hi Seq高通量测序比较分析来研究木质素取食对低等白蚁消化系统的影响。结果表明：木质素取食组内源性（前肠/唾液腺）酚氧化酶含量的显著大于大于对照组,内源及外源性纤维素酶活性均小于对照组,尤其BG酶活性显著小于对照组;1%木质素对乳白蚁取食有一定的抑制作用,其后肠细菌多样性及丰度较对照组有所下降,但无显著的组间群落结构差异;Metastat、LDA（LDA Effect Size）及组间T-检验属水平群落差异分析显示,乳白蚁后肠核心菌群（相对丰度〉1%）Dysgonomonas属、弧菌属在木质素取食组中相对丰度显著小于对照组,Candidatus Azobacteriodes属相对丰度则显著大于对照组,它们在木质素增量取食引起的细菌群落变化中起关键作用。 |
| Abstract_FL | The lignin metabolism is one of the key steps in lignocelluloses digestion of termites.The effect of incremental lignin feeding on the digestive system of lower termite Coptotermes formosanus Shiraki was investigated by comparative assays of the feeding amount,the phenoloxidase concentration,the cellulase activity and the 16s rRNA gene Illumina HiSeq sequencing of symbiotic bacteria community.The results showed that the concentration of the endogenous (foregut/salivary gland) phenoxidase was significantly higher in lignin feeding group;the activities of both the endogenous and exogenous (hindgut) cellulases were lower in lignin feeding group,especially the β-glucosidase activity was significantly lower in lignin feeding group.The 1％ (W/V) lignin had feeding inhibition on C.formosanus,and the estimated richness and diversity of bacteria community was comparatively lower in lignin feeding group,but no significant difference was showed in bacteria community structure between groups.The bacteria community difference between groups was statistically analyzed by Metastat、LDA Effect Size and T-test methods.And the result of dominant bacteria population (relative abundance ＞ 1％) in genus level of C.formosanus hindgut suggested that the relative abundances of genus Dysgonomona and Vibro were significantly higher in lignin feeding group;the relative abundance of genus Candidatus Azobacteriodes was significantly lower in lignin feeding group.These three bacteria genera played key role in symbiotic bacteria community variation induced by incremental lignin feeding. |
| Author | 曾文慧 钟俊鸿 刘炳荣 李秋剑 |
| AuthorAffiliation | 广东省生物资源应用研究所,广东省动物保护与资源利用重点实验室,广东省野生动物保护与利用公共实验室,广州510260 |
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| Author_FL | ZHONG Jun-Hong LI Qiu-Jian LIU Bing-Rong ZENG Wen-Hui |
| Author_FL_xml | – sequence: 1 fullname: ZENG Wen-Hui – sequence: 2 fullname: ZHONG Jun-Hong – sequence: 3 fullname: LIU Bing-Rong – sequence: 4 fullname: LI Qiu-Jian |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DocumentTitleAlternate | The effect of incremental lignin feeding on digestive system of Coptotermes formosanus Shiraki |
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| Keywords | phenoloxidase Coptotermes formosanus Shiraki 肠道微生物群落 16S rRNA基因高通量测序 cellulase intestinal symbiont 纤维素酶 台湾乳白蚁 酚氧化酶 16S rRNA gene Illumina HiSeq |
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| Notes | The lignin metabolism is one of the key steps in lignocelluloses digestion of termites. The effect of incremental lignin feeding on the digestive system of lower termite Coptotermes formosanus Shiraki was investigated by comparative assays of the feeding amount,the phenoloxidase concentration,the cellulase activity and the 16 s rRNA gene Illumina Hi Seq sequencing of symbiotic bacteria community. The results showed that the concentration of the endogenous（ foregut/salivary gland） phenoxidase was significantly higher in lignin feeding group; the activities of both the endogenous and exogenous（ hindgut） cellulases were lower in lignin feeding group,especially the β-glucosidase activity was significantly lower in lignin feeding group. The 1%（ W/V） lignin had feeding inhibition on C. formosanus,and the estimated richness and diversity of bacteria community was comparatively lower in lignin feeding group,but no significant difference was showed in bacteria community structure between groups. The bacteria community |
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| PublicationTitle | 环境昆虫学报 |
| PublicationTitleAlternate | Natural Enemies of Insects |
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| PublicationYear | 2017 |
| Publisher | 广东省生物资源应用研究所,广东省动物保护与资源利用重点实验室,广东省野生动物保护与利用公共实验室,广州510260 |
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| Snippet | 木质素代谢是白蚁消化木质纤维素的关键环节之一。本文利用木质素增量喂食台湾乳白蚁Coptotermes formosanus Shiraki,通过测定其取食量、体内酚氧化酶含量、纤维素酶活性的变... Q965%S433; 木质素代谢是白蚁消化木质纤维素的关键环节之一.本文利用木质素增量喂食台湾乳白蚁Coptotermes formosanus Shiraki,通过测定其取食量、体内酚氧化酶含量、纤维素... |
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| SubjectTerms | 16S rRNA基因高通量测序 台湾乳白蚁 纤维素酶 肠道微生物群落 酚氧化酶 |
| Title | 木质素增量取食对台湾乳白蚁消化系统的影响 |
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