电喷雾质谱结合化学计量学方法快速筛选玛咖促睾丸间质细胞增殖活性成分

应用电喷雾串联质谱法（ESI-MS/MS）对云南和秘鲁产玛咖样品中低极性化学成分进行检测,获得各样品的一级质谱指纹谱图和各离子峰的二级质谱数据,并测定各样品促进大鼠睾丸间质细胞增殖活性,采用化学计量学方法主成分分析（PCA）、偏最小二乘法（PLS）和灰色关联度分析（GRA）对所获得的一级质谱数据进行处理后,可有效区分玛咖的不同产地,且筛选出其中具有促大鼠睾丸间质细胞增殖活性的可能成分,进而通过二级质谱数据分析得到可能活性成分的结构。实验结果表明,玛咖低极性成分具有很好的促大鼠睾丸间质细胞增殖活性,其中活性较强的主要为N-benzylhexadecanamide和N-benzy-（9Z,12Z,...

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Bibliographic Details
Published in分析化学 Vol. 44; no. 11; pp. 1735 - 1741
Main Author 孙佳明 田淋淋 何忠梅 孟令文 孙慧 阚俊明 张辉
Format Journal Article
LanguageChinese
Published 长春中医药大学中医药与生物工程研究开发中心,长春,130117%吉林农业大学中药材学院,长春,130116%长春中医药大学研究生院,长春,130117 2016
Subjects
不同产地
促睾丸间质细胞增殖
化学计量学方法
玛咖
电喷雾串联质谱
Lepidium meyenii
Chemometrics method
玛咖
电喷雾串联质谱
促睾丸间质细胞增殖
Promoting leydig cell proliferation
不同产地
Electrospray ionization tandem mass spectrometry
Different geographical origin
化学计量学方法
Online AccessGet full text
ISSN0253-3820
DOI10.11895/j.issn.0253-3820.160232

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Summary:应用电喷雾串联质谱法（ESI-MS/MS）对云南和秘鲁产玛咖样品中低极性化学成分进行检测,获得各样品的一级质谱指纹谱图和各离子峰的二级质谱数据,并测定各样品促进大鼠睾丸间质细胞增殖活性,采用化学计量学方法主成分分析（PCA）、偏最小二乘法（PLS）和灰色关联度分析（GRA）对所获得的一级质谱数据进行处理后,可有效区分玛咖的不同产地,且筛选出其中具有促大鼠睾丸间质细胞增殖活性的可能成分,进而通过二级质谱数据分析得到可能活性成分的结构。实验结果表明,玛咖低极性成分具有很好的促大鼠睾丸间质细胞增殖活性,其中活性较强的主要为N-benzylhexadecanamide和N-benzy-（9Z,12Z,15Z）-octadecatrienamide。本方法为简单、快速分析中药中促睾丸间质细胞增殖活性成分筛选方法提供了借鉴。
Bibliography:The low-polarity constituents from Lepidium meyenii in Yunnan and Peru were detected by electrospray ionization tandem mass spectrometry（ESI-MS / MS）. The effects of 10 samples of Lepidium meyenii on proliferation of rat leydig cells were measured. After the process of MS data using principal component analysis（PCA） methods,the samples from Yunnan and Peru were distinguished effectively.Subsequently,the grey relational degree analysis（GRA） and partial least squares analysis（PLS） were employed to reveal the correlation between common peaks of ESI fingerprint of Lepidium meyenii from different geographical origins and proliferation activity of rat leydig cells. The results suggested that most low-polarity constituents from Lepidium meyenii had good activities on proliferation of rat leydig cells, in which N-benzylhexadecanamide and N-benzy-（9Z,12 Z,15Z）-octadecatrienamide exhibited the highest activities. In addition,the proposed procedure could also be used as a simple,quick reference method to screen the acti
ISSN:0253-3820
DOI:10.11895/j.issn.0253-3820.160232