High hydrostatic pressure pre-treatment of whey proteins enhances whey protein hydrolysate inhibition of oxidative stress and IL-8 secretion in intestinal epithelial cells

• Published in: Food & nutrition research Vol. 56; no. 1; pp. 17549 - 10
• Main Authors: Piccolomini, AndréF., Iskandar, MichèleM, Lands, LarryC, Kubow, Stan
• Format: Journal Article
• Language: English
• Published: Sweden Taylor & Francis 01.01.2012; Co-Action Publishing; Swedish Nutrition Foundation
• Subjects: anti-inflammatory; anti-inflammatory activity; anti-oxidant; antioksidanter; caco-2 cells; cell viability; dose response; high pressure treatment; hydrogen peroxide; hydrolysates; inflammation; interleukin-8; intestinal mucosa; myse; Original; oxidative stress; oxidative toxicity; pressurized whey protein isolate hydrolysates; protein hydrolysates; protein structure; proteinpulver; reactive oxygen species; secretion; whey protein isolate; anti-inflammatory; anti-oxidant; caco-2 cells; pressurized whey protein isolate hydrolysates; reactive oxygen species
• ISSN: 1654-6628; 1654-661X; 1654-661X
• DOI: 10.3402/fnr.v56i0.17549

Background : High hyperbaric pressure treatment of whey protein isolate (WPI) causes changes in the protein structure that enhances the anti-oxidant and anti-inflammatory effects of WPI. Objective : The aim of this study was to compare the anti-oxidant and anti-inflammatory effects of pressurized whey protein isolate (pWPI) vs. native WPI (nWPI) hydrolysates in Caco-2 cells exposed to hydrogen peroxide (H 2 O 2 ). Design : Cells were cultured with different concentrations of pWPI or nWPI hydrolysates either 1 h before or 1 h after H 2 O 2 . Cell viability, IL-8 secretion, intracellular reactive oxygen species (ROS), and the medium anti-oxidant capacity (FRAP assay) were measured. Results : Prior to and after H 2 O 2 exposure, pWPI and nWPI hydrolysates inhibited IL-8 secretion and ROS generation, and increased FRAP activity in a dose-dependent manner. The maximal inhibition of H 2 O 2 -induced IL-8 secretion was greater with 2000 µg mL −1 of pWPI (50%) vs. nWPI (30%) hydrolysates. At the latter concentration, inhibition of H 2 O 2 -induced ROS formation reached 76% for pWPI, which was greater than for nWPI hydrolysates (32.5%). Conclusion : These results suggest that WPI hydrolysates can alleviate inflammation and oxidative stress in intestinal cells exposed to oxidative injury, which is further enhanced by hyperbaric pressure pre-treatment of WPI.