Directed evolution of the CpcA biosynthetic pathway and optimization of conditions for CpcA production and its properties - Dong, Dalu; Pan, Hangtao; Yu, Ping (Applied microbiology and biotechnology, 2014)
Keywords: Amino acids; Analysis; Antioxidants; Bacterial proteins; beef extracts; Biomedical and Life Sciences; Biosynthesis; Biosynthetic Pathways; Biotechnologically Relevant Enzymes and Proteins; Biotechnology; carbon; Carbon - metabolism; Chemical engineering; Cloning; Culture Media - chemistry; Directed Molecular Evolution; E coli; Enzymes; Escherichia coli; evolution; Free radicals; Free Radicals - metabolism; Gene amplification; genes; Genetic aspects; Glycerol; Hydrogen-Ion Concentration; Industrial production; inhibitory concentration 50; Life Sciences; Metabolic Engineering; Microbial Genetics and Genomics; Microbiology; molecular weight; mutants; Mutation, Missense; Nickel; nitrogen; Nitrogen sources; Optimization; oxygen; Phycocyanin - biosynthesis; Phycocyanin - chemistry; Phycocyanin - genetics; Phycocyanin - isolation & purification; Point Mutation; polyacrylamide gel electrophoresis; polymerase chain reaction; Polypeptides; Properties; Protein Stability; response surface methodology; Sequence Alignment; sodium dodecyl sulfate; Spectrum Analysis; Spirulina - genetics; Spirulina - metabolism; Spirulina maxima; Studies; Sulfates; Temperature; Wavelengths; Yeast; yeast extract; China; Antioxidative activity; Directed evolution; Stability; CpcA; Error-prone PCR; Response surface methodology; Pathway engineering; Spectrum