固相萃取-高效液相色谱荧光检测法测定人血浆3-硝基酪氨酸含量

建立了人血浆中痕量3-硝基酪氨酸的高效液相色谱-荧光检测法。血浆经乙腈沉淀蛋白后，上清液用氮气吹干，残渣复溶后过C18固相萃取小柱净化浓缩，洗脱液再用氮气吹干，残渣进行荧光衍生化反应。采用高效液相色谱分离，荧光检测器检测，外标法定量。3-硝基酪氨酸在0．50～50．0 nmol／L范围内线性关系良好，相关系数为0．9999；样品加标绝对回收率为89．3％～91．9％，相对回收率为99．2％-107．9％；日内相对标准偏差为1．46％～4．79％，日间相对标准偏差为2．75％～9．20％；在血浆中检出限为0．25nmol／L；测定正常人血浆中3-硝基酪氨酸平均浓度为1．18nmol／L（n=21...

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Published inFēnxī huàxué Vol. 37; no. 2; pp. 242 - 246
Main Author 郭帅 邵丽华 荣琳 王希峰 郗德凤
Format Journal Article
LanguageChinese
Published 山东大学公共卫生学院,济南,250012 2009
Subjects
3-硝基酪氨酸
4-氟-7-硝基-2,1,3-苯并氧杂恶二唑
人血浆
固相萃取
荧光检测
高效液相色谱
人血浆
固相萃取
高效液相色谱
3-硝基酪氨酸
荧光检测
4-氟-7-硝基-2,1,3-苯并氧杂恶二唑
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ISSN0253-3820
DOI10.3321/j.issn:0253-3820.2009.02.015

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Abstract 建立了人血浆中痕量3-硝基酪氨酸的高效液相色谱-荧光检测法。血浆经乙腈沉淀蛋白后，上清液用氮气吹干，残渣复溶后过C18固相萃取小柱净化浓缩，洗脱液再用氮气吹干，残渣进行荧光衍生化反应。采用高效液相色谱分离，荧光检测器检测，外标法定量。3-硝基酪氨酸在0．50～50．0 nmol／L范围内线性关系良好，相关系数为0．9999；样品加标绝对回收率为89．3％～91．9％，相对回收率为99．2％-107．9％；日内相对标准偏差为1．46％～4．79％，日间相对标准偏差为2．75％～9．20％；在血浆中检出限为0．25nmol／L；测定正常人血浆中3-硝基酪氨酸平均浓度为1．18nmol／L（n=21），Ⅱ型糖尿病病人血浆中3-NT平均浓度为3．63nmol／L（n=23）。本方法灵敏度高，重现性好，适用于临床研究中大样本量的测定。
AbstractList 建立了人血浆中痕量3-硝基酪氨酸的高效液相色谱-荧光检测法。血浆经乙腈沉淀蛋白后，上清液用氮气吹干，残渣复溶后过C18固相萃取小柱净化浓缩，洗脱液再用氮气吹干，残渣进行荧光衍生化反应。采用高效液相色谱分离，荧光检测器检测，外标法定量。3-硝基酪氨酸在0．50～50．0 nmol／L范围内线性关系良好，相关系数为0．9999；样品加标绝对回收率为89．3％～91．9％，相对回收率为99．2％-107．9％；日内相对标准偏差为1．46％～4．79％，日间相对标准偏差为2．75％～9．20％；在血浆中检出限为0．25nmol／L；测定正常人血浆中3-硝基酪氨酸平均浓度为1．18nmol／L（n=21），Ⅱ型糖尿病病人血浆中3-NT平均浓度为3．63nmol／L（n=23）。本方法灵敏度高，重现性好，适用于临床研究中大样本量的测定。
O6; 建立了人血浆中痕量3-硝基酪氨酸的高效液相色谱-荧光检测法. 血浆经乙腈沉淀蛋白后,上清液用氮气吹干,残渣复溶后过C18固相萃取小柱净化浓缩,洗脱液再用氮气吹干,残渣进行荧光衍生化反应.采用高效液相色谱分离,荧光检测器检测,外标法定量.3-硝基酪氨酸在0.50～50.0 nmol/L范围内线性关系良好,相关系数为0.9999;样品加标绝对回收率为89.3%～91.9%,相对回收率为99.2%～107.9%;日内相对标准偏差为1.46%～4.79%,日间相对标准偏差为2.75%～9.20%;在血浆中检出限为0.25 nmol/L;测定正常人血浆中3-硝基酪氨酸平均浓度为118 nmol/L(n=21),Ⅱ型糖尿病病人血浆中3-NT平均浓度为3.63 nmol/L(n=23).本方法灵敏度高,重现性好,适用于临床研究中大样本量的测定.
Author 郭帅 邵丽华 荣琳 王希峰 郗德凤
AuthorAffiliation 山东大学公共卫生学院,济南250012
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GUO Shuai
RONG Lin
WANG Xi-Feng
SHAO Li-Hua
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Issue 2
Keywords 人血浆
固相萃取
高效液相色谱
3-硝基酪氨酸
荧光检测
4-氟-7-硝基-2,1,3-苯并氧杂恶二唑
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Notes 3-Nitrotyrosine, 4-fluoro-7-nitrobenzofurazan, human plasma, fluorescence detection, solid phaseextraction, high performance liquid chromatography
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PublicationTitleAlternate Chinese Journal of Analytical Chemistry
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PublicationYear 2009
Publisher 山东大学公共卫生学院,济南,250012
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Snippet 建立了人血浆中痕量3-硝基酪氨酸的高效液相色谱-荧光检测法。血浆经乙腈沉淀蛋白后，上清液用氮气吹干，残渣复溶后过C18固相萃取小柱净化浓缩，洗脱液再用氮气吹干，残渣进...
O6; 建立了人血浆中痕量3-硝基酪氨酸的高效液相色谱-荧光检测法. 血浆经乙腈沉淀蛋白后,上清液用氮气吹干,残渣复溶后过C18固相萃取小柱净化浓缩,洗脱液再用氮气吹干,残渣...
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StartPage 242
SubjectTerms 3-硝基酪氨酸
4-氟-7-硝基-2,1,3-苯并氧杂恶二唑
人血浆
固相萃取
荧光检测
高效液相色谱
Title 固相萃取-高效液相色谱荧光检测法测定人血浆3-硝基酪氨酸含量
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