miR-320-3p调控脂肪细胞分化的研究

目的：探讨miR-320-3p对脂肪细胞分化的影响。方法分离并培养小鼠骨髓间充质干细胞（MSCs），并对其进行脂肪细胞诱导分化3 d，用qRT-PCR检测miR-320-3p在成脂分化过程中的表达水平。在骨髓基质细胞系ST2中转染miR-320-3p，对其进行成脂方向诱导分化，用油红O染色和qRT-PCR检测miR-320-3p对ST2成脂分化的影响。结果 MSCs向脂肪细胞分化过程中miR-320-3p表达增加（P〈0.01）；与转染阴性对照NC mimics相比，ST2细胞转染miR-320-3p mimics后油红O染色显示脂肪细胞明显增多，脂肪细胞特异性转录因子过氧化物酶体增殖物激活受...

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Bibliographic Details
Published in天津医药 Vol. 43; no. 12; pp. 1353 - 1355
Main Author 王昌兰 高志红 常爱玲 王宝利
Format Journal Article
LanguageChinese
Published 天津医科大学总医院内分泌科%天津医科大学总医院内分泌科%天津医科大学代谢病医院内分泌研究所,卫生部激素与发育重点实验室 邮编,300070 2015
天津医科大学代谢病医院内分泌研究所，卫生部激素与发育重点实验室 邮编300070
Subjects
miR-320-3p
微RNAs
成脂分化
细胞分化
脂细胞
间质干细胞
成脂分化
microRNAs
cell differentiation
adipogenesis
细胞分化
mesenchymal stem cells
miR-320-3p
adipocytes
脂细胞
间质干细胞
微RNAs
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ISSN0253-9896
DOI10.11958/j.issn.0253-9896.2015.12.003

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Summary:目的：探讨miR-320-3p对脂肪细胞分化的影响。方法分离并培养小鼠骨髓间充质干细胞（MSCs），并对其进行脂肪细胞诱导分化3 d，用qRT-PCR检测miR-320-3p在成脂分化过程中的表达水平。在骨髓基质细胞系ST2中转染miR-320-3p，对其进行成脂方向诱导分化，用油红O染色和qRT-PCR检测miR-320-3p对ST2成脂分化的影响。结果 MSCs向脂肪细胞分化过程中miR-320-3p表达增加（P〈0.01）；与转染阴性对照NC mimics相比，ST2细胞转染miR-320-3p mimics后油红O染色显示脂肪细胞明显增多，脂肪细胞特异性转录因子过氧化物酶体增殖物激活受体γ（PPARγ）、CCAAT增强子结合蛋白α（C/EBPα）和脂肪细胞脂肪酸结合蛋白4（FABP4）基因表达显著升高，差异有统计学意义（P〈0.05）。结论 miR-320-3p可促进ST2向脂肪细胞分化。
Bibliography:adipocytes;mesenchymal stem cells;cell differentiation;adipogenesis;microRNAs;miR-320-3p
Objective To study the role of miR-320-3p in adipocyte differentiation. Methods Marrow mesenchymal stem cells were isolated from mice and cultured then induced with adipogenic agents for 3 days. The transcription level of miR-320-3p was examined by qRT-PCR. Stromal ST2 cells were transfected with miR-320-3p, followed by adipogenic treatment. Oil-red O staining and qRT-PCR were employed to assess the differentiation of adipocytes induced by miR-320-3p. Results The expression level of miR-320-3p increased in MSCs after adipogenic treatment （P 〈 0.01）. Addition of miR-320-3p in ST2 cells promoted the formation of oil-red O positive adipocytes and up-regulated the expression levels of adipogenic transcription factors such as peroxisome proliferator-activated receptor γ （PPARγ）, CCAAT/enhancer binding protein-α（C/EBPα） and the marker gene adipocyte fatty acid binding protein 4 （FABP4）,compared to cells that transfected with miR
ISSN:0253-9896
DOI:10.11958/j.issn.0253-9896.2015.12.003