Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha
Marchantia polymorpha is one of the model species of basal land plants. Although CRISPR/Cas9-based genome editing has already been demonstrated for this plant, the efficiency was too low to apply to functional analysis. In this study, we show the establishment of CRISPR/Cas9 genome editing vectors w...
Saved in:
| Published in | PloS one Vol. 13; no. 10; p. e0205117 |
|---|---|
| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Public Library of Science
31.10.2018
Public Library of Science (PLoS) |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1932-6203 1932-6203 |
| DOI | 10.1371/journal.pone.0205117 |
Cover
| Abstract | Marchantia polymorpha is one of the model species of basal land plants. Although CRISPR/Cas9-based genome editing has already been demonstrated for this plant, the efficiency was too low to apply to functional analysis. In this study, we show the establishment of CRISPR/Cas9 genome editing vectors with high efficiency for both construction and genome editing. Codon optimization of Cas9 to Arabidopsis achieved over 70% genome editing efficiency at two loci tested. Systematic assessment revealed that guide sequences of 17 nt or shorter dramatically decreased this efficiency. We also demonstrated that a combinatorial use of this system and a floxed complementation construct enabled conditional analysis of a nearly essential gene. This study reports that simple, rapid, and efficient genome editing is feasible with the series of developed vectors. |
|---|---|
| AbstractList | Marchantia polymorpha is one of the model species of basal land plants. Although CRISPR/Cas9-based genome editing has already been demonstrated for this plant, the efficiency was too low to apply to functional analysis. In this study, we show the establishment of CRISPR/Cas9 genome editing vectors with high efficiency for both construction and genome editing. Codon optimization of Cas9 to Arabidopsis achieved over 70% genome editing efficiency at two loci tested. Systematic assessment revealed that guide sequences of 17 nt or shorter dramatically decreased this efficiency. We also demonstrated that a combinatorial use of this system and a floxed complementation construct enabled conditional analysis of a nearly essential gene. This study reports that simple, rapid, and efficient genome editing is feasible with the series of developed vectors. Marchantia polymorpha is one of the model species of basal land plants. Although CRISPR/Cas9-based genome editing has already been demonstrated for this plant, the efficiency was too low to apply to functional analysis. In this study, we show the establishment of CRISPR/Cas9 genome editing vectors with high efficiency for both construction and genome editing. Codon optimization of Cas9 to Arabidopsis achieved over 70% genome editing efficiency at two loci tested. Systematic assessment revealed that guide sequences of 17 nt or shorter dramatically decreased this efficiency. We also demonstrated that a combinatorial use of this system and a floxed complementation construct enabled conditional analysis of a nearly essential gene. This study reports that simple, rapid, and efficient genome editing is feasible with the series of developed vectors.Marchantia polymorpha is one of the model species of basal land plants. Although CRISPR/Cas9-based genome editing has already been demonstrated for this plant, the efficiency was too low to apply to functional analysis. In this study, we show the establishment of CRISPR/Cas9 genome editing vectors with high efficiency for both construction and genome editing. Codon optimization of Cas9 to Arabidopsis achieved over 70% genome editing efficiency at two loci tested. Systematic assessment revealed that guide sequences of 17 nt or shorter dramatically decreased this efficiency. We also demonstrated that a combinatorial use of this system and a floxed complementation construct enabled conditional analysis of a nearly essential gene. This study reports that simple, rapid, and efficient genome editing is feasible with the series of developed vectors. Marchantia polymorpha is one of the model species of basal land plants. Although CRISPR/Cas9-based genome editing has already been demonstrated for this plant, the efficiency was too low to apply to functional analysis. In this study, we show the establishment of CRISPR/Cas9 genome editing vectors with high efficiency for both construction and genome editing. Codon optimization of Cas9 to Arabidopsis achieved over 70% genome editing efficiency at two loci tested. Systematic assessment revealed that guide sequences of 17 nt or shorter dramatically decreased this efficiency. We also demonstrated that a combinatorial use of this system and a floxed complementation construct enabled conditional analysis of a nearly essential gene. This study reports that simple, rapid, and efficient genome editing is feasible with the series of developed vectors. |
| Author | Matsuda, Yoriko Sugano, Shigeo S. Shirakawa, Makoto Osakabe, Keishi Ishida, Sakiko Shimada, Tomoo Hara-Nishimura, Ikuko Nishihama, Ryuichi Takagi, Junpei Kohchi, Takayuki |
| AuthorAffiliation | 1 R-GIRO, Ritsumeikan University, Kusatsu, Shiga, Japan University of Tsukuba, JAPAN 3 Graduate School of Biostudies, Kyoto University, Kyoto, Japan 2 JST, PRESTO, Kawaguchi, Saitama, Japan 4 Graduate School of Science, Kyoto University, Kyoto, Japan 5 Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima, Tokushima, Japan |
| AuthorAffiliation_xml | – name: 1 R-GIRO, Ritsumeikan University, Kusatsu, Shiga, Japan – name: 2 JST, PRESTO, Kawaguchi, Saitama, Japan – name: 5 Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima, Tokushima, Japan – name: University of Tsukuba, JAPAN – name: 3 Graduate School of Biostudies, Kyoto University, Kyoto, Japan – name: 4 Graduate School of Science, Kyoto University, Kyoto, Japan |
| Author_xml | – sequence: 1 givenname: Shigeo S. surname: Sugano fullname: Sugano, Shigeo S. – sequence: 2 givenname: Ryuichi orcidid: 0000-0002-7032-732X surname: Nishihama fullname: Nishihama, Ryuichi – sequence: 3 givenname: Makoto surname: Shirakawa fullname: Shirakawa, Makoto – sequence: 4 givenname: Junpei surname: Takagi fullname: Takagi, Junpei – sequence: 5 givenname: Yoriko surname: Matsuda fullname: Matsuda, Yoriko – sequence: 6 givenname: Sakiko surname: Ishida fullname: Ishida, Sakiko – sequence: 7 givenname: Tomoo surname: Shimada fullname: Shimada, Tomoo – sequence: 8 givenname: Ikuko surname: Hara-Nishimura fullname: Hara-Nishimura, Ikuko – sequence: 9 givenname: Keishi surname: Osakabe fullname: Osakabe, Keishi – sequence: 10 givenname: Takayuki orcidid: 0000-0002-9712-4872 surname: Kohchi fullname: Kohchi, Takayuki |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/30379827$$D View this record in MEDLINE/PubMed |
| BookMark | eNp9Uk1vEzEUtFARbQP_AIElLlw29cfam-WAhKICkYpABc7WW9ubOHLsxd6A8u9xmrRqK8TJ1vPMeN57c45OQgwWoZeUTClv6MU6blMAPx1KeUoYEZQ2T9AZbTmrJCP85N79FJ3nvCZE8JmUz9ApJ7xpZ6w5Q38u-95pZ8OI59eL79-uL-aQ26qDbA1e2hA3FlvjRheWGILBbswYhsE7DaOLAY8R6xj2gFjM7Bl2dLpAwe-yy9gF_AWSXkEYHeAh-t0mpmEFz9HTHny2L47nBP38ePlj_rm6-vppMf9wVWnRkLHqTdtr2_GWAGdMGyJ535C6aWjNrJUzo5mVjHLTCC24Jo0V0jDeCbBANTF8gl4fdAcfszrOLCtGWSOFEJIXxOKAMBHWakhuA2mnIjh1U4hpqSCVnrxVbc8kY4ZBDbJuKQDvWkug7mopJCGkaL0__rbtNtboMtYE_oHow5fgVmoZf6uypJbKWRF4exRI8dfW5lFtXNbWewg2bg--W8FE2ewEvXkE_Xd3r-47urNym4ACqA8AnWLOyfZ3EErUPmi3smofNHUMWqG9e0TTbryJROnL-f-T_wK8Bd19 |
| CitedBy_id | crossref_primary_10_1016_j_cub_2020_07_016 crossref_primary_10_1111_nph_16132 crossref_primary_10_1016_j_cub_2019_07_084 crossref_primary_10_1038_s41467_024_55728_3 crossref_primary_10_1371_journal_pone_0269984 crossref_primary_10_1038_s41477_020_00784_y crossref_primary_10_1016_j_cub_2023_09_020 crossref_primary_10_1038_s42003_023_05746_6 crossref_primary_10_3389_fpls_2019_00935 crossref_primary_10_5511_plantbiotechnology_22_0704a crossref_primary_10_1186_s13059_021_02476_y crossref_primary_10_1038_s41467_020_19978_1 crossref_primary_10_1016_j_cub_2020_05_081 crossref_primary_10_1016_j_cub_2023_08_073 crossref_primary_10_7717_peerj_10174 crossref_primary_10_1038_s41477_024_01669_0 crossref_primary_10_1111_nph_18385 crossref_primary_10_1111_tpj_16530 crossref_primary_10_1111_nph_17850 crossref_primary_10_1083_jcb_202203139 crossref_primary_10_7554_eLife_57088 crossref_primary_10_1111_pce_13869 crossref_primary_10_1016_j_cub_2023_02_022 crossref_primary_10_7554_eLife_57090 crossref_primary_10_1093_pcp_pcz232 crossref_primary_10_1016_j_celrep_2024_114696 crossref_primary_10_1016_j_cub_2024_01_008 crossref_primary_10_1093_pcp_pcab126 crossref_primary_10_1093_plcell_koac219 crossref_primary_10_1242_dev_202928 crossref_primary_10_1093_pcp_pcae002 crossref_primary_10_1093_jxb_erae256 crossref_primary_10_1111_nph_19981 crossref_primary_10_1007_s13205_018_1563_x crossref_primary_10_1093_pcp_pcad159 crossref_primary_10_1073_pnas_1912741116 crossref_primary_10_1007_s13237_024_00498_y crossref_primary_10_1002_advs_202206433 crossref_primary_10_1093_pcp_pcae080 crossref_primary_10_3389_fpls_2024_1477894 crossref_primary_10_1016_j_devcel_2024_12_027 crossref_primary_10_1016_j_cub_2023_10_054 crossref_primary_10_1038_s44318_024_00181_7 crossref_primary_10_3389_fmicb_2020_609376 crossref_primary_10_1111_nph_16997 crossref_primary_10_1038_s41588_024_02071_4 crossref_primary_10_3389_fpls_2023_1179394 crossref_primary_10_3389_fpls_2023_1275503 crossref_primary_10_1038_s41598_020_65372_8 crossref_primary_10_1111_nph_18815 crossref_primary_10_1016_j_indcrop_2020_112146 crossref_primary_10_1111_nph_20169 crossref_primary_10_1093_pcp_pcad043 crossref_primary_10_1093_pcp_pcac076 crossref_primary_10_1016_j_cub_2021_10_023 crossref_primary_10_1111_tpj_15787 crossref_primary_10_7554_eLife_58396 crossref_primary_10_3389_fpls_2022_1051017 crossref_primary_10_1371_journal_pgen_1011468 crossref_primary_10_1038_s41477_024_01703_1 crossref_primary_10_1093_plcell_koab106 crossref_primary_10_1093_aob_mcaa063 crossref_primary_10_1093_plphys_kiae613 crossref_primary_10_1016_j_cub_2023_06_015 crossref_primary_10_1093_pcp_pcac004 crossref_primary_10_1093_pcp_pcae029 crossref_primary_10_1111_nph_19687 crossref_primary_10_1016_j_bbrc_2020_11_063 crossref_primary_10_1146_annurev_arplant_082520_094256 crossref_primary_10_1016_j_cub_2023_05_050 crossref_primary_10_1186_s12936_023_04665_5 crossref_primary_10_1016_j_cub_2024_09_061 crossref_primary_10_5511_plantbiotechnology_22_0328a crossref_primary_10_3389_fcell_2022_883491 crossref_primary_10_3389_fpls_2020_00007 crossref_primary_10_1016_j_cub_2024_04_002 crossref_primary_10_1016_j_cub_2021_06_010 crossref_primary_10_2139_ssrn_3936040 crossref_primary_10_1093_pcp_pcz029 crossref_primary_10_1093_pcp_pcab042 crossref_primary_10_1093_plcell_koae206 crossref_primary_10_1016_j_cell_2024_06_039 crossref_primary_10_7554_eLife_79258 crossref_primary_10_1073_pnas_2322211121 crossref_primary_10_1111_nph_17653 crossref_primary_10_1016_j_bbabio_2020_148309 crossref_primary_10_3389_fpls_2022_915268 crossref_primary_10_1038_s41477_019_0466_0 crossref_primary_10_1038_s41467_024_55278_8 crossref_primary_10_1186_s43141_020_00036_8 crossref_primary_10_1073_pnas_2117803119 crossref_primary_10_1038_s41477_020_0662_y crossref_primary_10_1007_s10265_020_01180_5 crossref_primary_10_3389_fpls_2020_00997 crossref_primary_10_1016_j_cub_2023_06_083 crossref_primary_10_1007_s00497_021_00409_0 crossref_primary_10_1016_j_celrep_2022_110975 crossref_primary_10_15252_embj_2018100240 crossref_primary_10_5511_plantbiotechnology_21_1219a crossref_primary_10_1134_S002689332206019X crossref_primary_10_1016_j_cub_2024_01_013 crossref_primary_10_3389_fpls_2022_979066 crossref_primary_10_1093_pcp_pcab176 crossref_primary_10_1093_pcp_pcad079 crossref_primary_10_1111_nph_18854 crossref_primary_10_1016_j_cub_2024_01_014 crossref_primary_10_1111_tpj_17236 crossref_primary_10_1007_s10265_021_01345_w crossref_primary_10_3389_fpls_2022_952820 crossref_primary_10_1016_j_cub_2022_12_062 crossref_primary_10_1111_ppl_13898 crossref_primary_10_1093_pcp_pcz187 crossref_primary_10_7717_peerj_12419 crossref_primary_10_1016_j_celrep_2024_114463 crossref_primary_10_1093_pcp_pcad118 crossref_primary_10_1016_j_celrep_2024_114466 crossref_primary_10_1016_j_cpb_2020_100152 crossref_primary_10_1111_nph_18046 crossref_primary_10_1111_nph_15294 crossref_primary_10_1093_pcp_pcae055 crossref_primary_10_1016_j_cub_2021_08_050 crossref_primary_10_1021_acssynbio_9b00511 crossref_primary_10_1093_plcell_koac367 crossref_primary_10_1093_plcell_koad216 crossref_primary_10_1016_j_cub_2023_07_068 crossref_primary_10_1038_s41477_021_00888_z crossref_primary_10_3389_fpls_2021_657548 crossref_primary_10_3389_fpls_2023_1108027 crossref_primary_10_5511_plantbiotechnology_21_1217b crossref_primary_10_1016_j_cub_2019_10_004 crossref_primary_10_1038_s41477_022_01325_5 crossref_primary_10_1007_s00425_023_04247_4 crossref_primary_10_5511_plantbiotechnology_22_0810a crossref_primary_10_1093_pcp_pcz212 |
| Cites_doi | 10.1105/tpc.110.077164 10.1093/bioinformatics/btt764 10.1111/tpj.12554 10.1105/tpc.106.048298 10.1093/pcp/pcw173 10.1007/s10265-014-0695-2 10.1534/g3.116.035204 10.1038/nprot.2015.140 10.1104/pp.114.245100 10.1093/pcp/pcx095 10.1086/329782 10.1093/pcp/pcu014 10.1186/s13007-017-0167-5 10.1371/journal.pone.0138876 10.1093/pcp/pcu154 10.1371/journal.pgen.1005084 10.1263/jbb.104.34 10.1126/science.1258096 10.4161/cc.27508 10.1016/j.cell.2016.08.031 10.1016/j.pbi.2016.11.011 10.1073/pnas.1400822111 10.1093/pcp/pcv182 10.1016/j.tplants.2014.10.001 10.1007/s11103-015-0342-x 10.1093/pcp/pcv102 10.1271/bbb.120700 10.1007/s11103-014-0266-x 10.1089/hum.2015.29002.fzh 10.1093/pcp/pcv097 10.1038/srep01532 10.1126/science.1225829 10.1038/mtna.2014.12 10.1038/nbt.2969 10.1073/pnas.1710597114 10.1016/0014-4827(68)90403-5 10.1186/s12864-016-3331-9 10.1093/pcp/pcu170 10.1038/nbt.2808 10.1371/journal.pgen.1005207 10.1093/pcp/pcn085 10.1002/0471143030.cb1912s44 10.1016/S1360-1385(02)02302-6 10.1093/pcp/pcw020 10.1016/j.cell.2017.09.030 10.1126/science.1231143 10.1105/tpc.113.117051 10.1038/nplants.2017.107 10.1105/tpc.17.00659 |
| ContentType | Journal Article |
| Copyright | 2018 Sugano et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2018 Sugano et al 2018 Sugano et al |
| Copyright_xml | – notice: 2018 Sugano et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: 2018 Sugano et al 2018 Sugano et al |
| DBID | AAYXX CITATION NPM 3V. 7QG 7QL 7QO 7RV 7SN 7SS 7T5 7TG 7TM 7U9 7X2 7X7 7XB 88E 8AO 8C1 8FD 8FE 8FG 8FH 8FI 8FJ 8FK ABJCF ABUWG AEUYN AFKRA ARAPS ATCPS AZQEC BBNVY BENPR BGLVJ BHPHI C1K CCPQU D1I DWQXO FR3 FYUFA GHDGH GNUQQ H94 HCIFZ K9. KB. KB0 KL. L6V LK8 M0K M0S M1P M7N M7P M7S NAPCQ P5Z P62 P64 PATMY PDBOC PHGZM PHGZT PIMPY PJZUB PKEHL PPXIY PQEST PQGLB PQQKQ PQUKI PRINS PTHSS PYCSY RC3 7X8 5PM DOA |
| DOI | 10.1371/journal.pone.0205117 |
| DatabaseName | CrossRef PubMed ProQuest Central (Corporate) Animal Behavior Abstracts Bacteriology Abstracts (Microbiology B) Biotechnology Research Abstracts Nursing & Allied Health Database Ecology Abstracts Entomology Abstracts (Full archive) Immunology Abstracts Meteorological & Geoastrophysical Abstracts Nucleic Acids Abstracts Virology and AIDS Abstracts Agricultural Science Collection ProQuest Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) ProQuest Pharma Collection Public Health Database Technology Research Database ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Collection Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Materials Science & Engineering ProQuest Central (Alumni) ProQuest One Sustainability ProQuest Central UK/Ireland Advanced Technologies & Aerospace Collection Agricultural & Environmental Science Collection ProQuest Central Essentials - QC Biological Science Collection ProQuest Central Technology Collection (via ProQuest SciTech Premium Collection) Natural Science Collection Environmental Sciences and Pollution Management ProQuest One Community College ProQuest Materials Science Collection ProQuest Central Engineering Research Database Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student AIDS and Cancer Research Abstracts SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) Materials Science Database Nursing & Allied Health Database (Alumni Edition) Meteorological & Geoastrophysical Abstracts - Academic ProQuest Engineering Collection Biological Sciences Agricultural Science Database ProQuest Health & Medical Collection PML(ProQuest Medical Library) Algology Mycology and Protozoology Abstracts (Microbiology C) ProQuest Biological Science Engineering Database Nursing & Allied Health Premium Advanced Technologies & Aerospace Database ProQuest Advanced Technologies & Aerospace Collection Biotechnology and BioEngineering Abstracts Environmental Science Database Materials Science Collection ProQuest Central Premium ProQuest One Academic (New) ProQuest Publicly Available Content Database ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China Engineering collection Environmental Science Collection Genetics Abstracts MEDLINE - Academic PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals |
| DatabaseTitle | CrossRef PubMed Agricultural Science Database Publicly Available Content Database ProQuest Central Student ProQuest Advanced Technologies & Aerospace Collection ProQuest Central Essentials Nucleic Acids Abstracts SciTech Premium Collection ProQuest Central China Environmental Sciences and Pollution Management ProQuest One Applied & Life Sciences ProQuest One Sustainability Health Research Premium Collection Meteorological & Geoastrophysical Abstracts Natural Science Collection Health & Medical Research Collection Biological Science Collection ProQuest Central (New) ProQuest Medical Library (Alumni) Engineering Collection Advanced Technologies & Aerospace Collection Engineering Database Virology and AIDS Abstracts ProQuest Biological Science Collection ProQuest One Academic Eastern Edition Agricultural Science Collection ProQuest Hospital Collection ProQuest Technology Collection Health Research Premium Collection (Alumni) Biological Science Database Ecology Abstracts ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts Environmental Science Collection Entomology Abstracts Nursing & Allied Health Premium ProQuest Health & Medical Complete ProQuest One Academic UKI Edition Environmental Science Database ProQuest Nursing & Allied Health Source (Alumni) Engineering Research Database ProQuest One Academic Meteorological & Geoastrophysical Abstracts - Academic ProQuest One Academic (New) Technology Collection Technology Research Database ProQuest One Academic Middle East (New) Materials Science Collection ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest One Health & Nursing ProQuest Natural Science Collection ProQuest Pharma Collection ProQuest Central ProQuest Health & Medical Research Collection Genetics Abstracts ProQuest Engineering Collection Biotechnology Research Abstracts Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) Agricultural & Environmental Science Collection AIDS and Cancer Research Abstracts Materials Science Database ProQuest Materials Science Collection ProQuest Public Health ProQuest Nursing & Allied Health Source ProQuest SciTech Collection Advanced Technologies & Aerospace Database ProQuest Medical Library Animal Behavior Abstracts Materials Science & Engineering Collection Immunology Abstracts ProQuest Central (Alumni) MEDLINE - Academic |
| DatabaseTitleList | PubMed MEDLINE - Academic Agricultural Science Database |
| Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: 8FG name: ProQuest Technology Collection url: https://search.proquest.com/technologycollection1 sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Sciences (General) |
| DocumentTitleAlternate | Efficient genome editing and its application to conditional genetic analysis in M. polymorpha |
| EISSN | 1932-6203 |
| ExternalDocumentID | 2127655563 oai_doaj_org_article_9f2622d2a4a6491aa3b9e0a4b4656000 PMC6209168 30379827 10_1371_journal_pone_0205117 |
| Genre | Research Support, Non-U.S. Gov't Journal Article |
| GeographicLocations | Japan |
| GeographicLocations_xml | – name: Japan |
| GrantInformation_xml | – fundername: ; grantid: JP22000014 – fundername: ; – fundername: ; grantid: JP17H07424 – fundername: ; grantid: JP20726313 – fundername: ; grantid: 11106 – fundername: ; grantid: 15J07657 – fundername: ; grantid: KP25113009 – fundername: ; grantid: 24005453 |
| GroupedDBID | --- 123 29O 2WC 53G 5VS 7RV 7X2 7X7 7XC 88E 8AO 8C1 8CJ 8FE 8FG 8FH 8FI 8FJ A8Z AAFWJ AAUCC AAWOE AAYXX ABDBF ABIVO ABJCF ABUWG ACGFO ACIHN ACIWK ACPRK ACUHS ADBBV ADRAZ AEAQA AENEX AEUYN AFKRA AFPKN AFRAH AHMBA ALIPV ALMA_UNASSIGNED_HOLDINGS AOIJS APEBS ARAPS ATCPS BAWUL BBNVY BCNDV BENPR BGLVJ BHPHI BKEYQ BPHCQ BVXVI BWKFM CCPQU CITATION CS3 D1I D1J D1K DIK DU5 E3Z EAP EAS EBD EMOBN ESX EX3 F5P FPL FYUFA GROUPED_DOAJ GX1 HCIFZ HH5 HMCUK HYE IAO IEA IGS IHR IHW INH INR IOV IPY ISE ISR ITC K6- KB. KQ8 L6V LK5 LK8 M0K M1P M48 M7P M7R M7S M~E NAPCQ O5R O5S OK1 OVT P2P P62 PATMY PDBOC PHGZM PHGZT PIMPY PQQKQ PROAC PSQYO PTHSS PV9 PYCSY RNS RPM RZL SV3 TR2 UKHRP WOQ WOW ~02 ~KM BBORY IPNFZ NPM RIG 3V. 7QG 7QL 7QO 7SN 7SS 7T5 7TG 7TM 7U9 7XB 8FD 8FK AZQEC C1K DWQXO FR3 GNUQQ H94 K9. KL. M7N P64 PJZUB PKEHL PPXIY PQEST PQGLB PQUKI PRINS RC3 7X8 ESTFP PUEGO 5PM - 02 AAPBV ABPTK ADACO BBAFP KM |
| ID | FETCH-LOGICAL-c570t-fd9fceb390a322cd063f70477142ee68dc2e6213d75c53c07e56d23b5aea1c0d3 |
| IEDL.DBID | M48 |
| ISSN | 1932-6203 |
| IngestDate | Thu Nov 25 14:37:28 EST 2021 Wed Aug 27 01:28:25 EDT 2025 Thu Aug 21 18:33:43 EDT 2025 Sat Sep 27 23:44:19 EDT 2025 Fri Jul 25 10:26:25 EDT 2025 Thu Apr 03 07:03:10 EDT 2025 Tue Jul 01 02:09:03 EDT 2025 Thu Apr 24 23:04:28 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 10 |
| Language | English |
| License | This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Creative Commons Attribution License |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c570t-fd9fceb390a322cd063f70477142ee68dc2e6213d75c53c07e56d23b5aea1c0d3 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Competing Interests: This study was partly funded by a donation from Otsuka Pharmaceuticals to Tokushima University. There are no patents, products in development or marketed products to declare. This does not alter our adherence to all the PLOS ONE policies on sharing data and materials, as detailed online in the guide for authors. |
| ORCID | 0000-0002-7032-732X 0000-0002-9712-4872 |
| OpenAccessLink | https://www.proquest.com/docview/2127655563?pq-origsite=%requestingapplication%&accountid=15518 |
| PMID | 30379827 |
| PQID | 2127655563 |
| PQPubID | 1436336 |
| ParticipantIDs | plos_journals_2127655563 doaj_primary_oai_doaj_org_article_9f2622d2a4a6491aa3b9e0a4b4656000 pubmedcentral_primary_oai_pubmedcentral_nih_gov_6209168 proquest_miscellaneous_2127952519 proquest_journals_2127655563 pubmed_primary_30379827 crossref_primary_10_1371_journal_pone_0205117 crossref_citationtrail_10_1371_journal_pone_0205117 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2018-10-31 |
| PublicationDateYYYYMMDD | 2018-10-31 |
| PublicationDate_xml | – month: 10 year: 2018 text: 2018-10-31 day: 31 |
| PublicationDecade | 2010 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States – name: San Francisco – name: San Francisco, CA USA |
| PublicationTitle | PloS one |
| PublicationTitleAlternate | PLoS One |
| PublicationYear | 2018 |
| Publisher | Public Library of Science Public Library of Science (PLoS) |
| Publisher_xml | – name: Public Library of Science – name: Public Library of Science (PLoS) |
| References | A Komatsu (ref33) 2014; 166 M Endo (ref42) 2015; 56 K Kosetsu (ref46) 2010; 22 L Cong (ref48) 2013; 339 H Kato (ref37) 2017; 58 Y Wang (ref13) 2014; 32 T Sakuma (ref50) 2016; 11 M Tsuzuki (ref52) 2016; 57 M Jinek (ref3) 2012; 337 J Ma (ref41) 2014; 13 SS Sugano (ref25) 2014; 55 E Flores-Sandoval (ref36) 2015; 11 A Ferenczi (ref11) 2017; 114 K Ishizaki (ref22) 2013; 3 F Zhang (ref2) 2015; 26 A Kubota (ref20) 2013; 77 M Group (ref43) 2002; 7 Y Fu (ref40) 2014; 32 M Mikami (ref26) 2015; 88 A Greiner (ref10) 2017; 29 F Fauser (ref12) 2014; 79 T Aida (ref49) 2016; 17 T Nomura (ref8) 2016; 57 JA Doudna (ref1) 2014; 346 H Puchta (ref5) 2016; 36 K Ishizaki (ref18) 2016; 57 B Hall (ref28) 2009 J Xu (ref44) 2015; 20 Y Osakabe (ref7) 2015; 56 M Lopez-Obando (ref9) 2016 T Nakagawa (ref31) 2007; 104 H Ma (ref38) 2014; 3 R Nishihama (ref23) 2016; 57 S Tsuboyama-Tanaka (ref21) 2015; 128 K Yin (ref6) 2017; 3 F Althoff (ref34) 2013 K Ishizaki (ref30) 2015; 10 OL Gamborg (ref29) 1968; 50 K Ishizaki (ref19) 2008; 49 RA Johnson (ref27) 2015; 87 H Shen (ref47) 2017; 7 JL Bowman (ref16) 2017; 171 CR Barnes (ref17) 1908; 46 A Xiao (ref32) 2014; 30 PC Lin (ref51) 2016; 57 H Kato (ref35) 2015; 11 K Ishizaki (ref39) 2013; 25 S Kopischke (ref24) 2017; 13 Z Feng (ref14) 2014; 111 T Gaj (ref4) 2016; 8 H Wang (ref45) 2007; 19 C Chang (ref15) 2016; 167 |
| References_xml | – volume: 22 start-page: 3778 issue: 11 year: 2010 ident: ref46 article-title: The MAP kinase MPK4 is required for cytokinesis in Arabidopsis thaliana publication-title: Plant Cell doi: 10.1105/tpc.110.077164 – volume: 30 start-page: 1180 issue: 8 year: 2014 ident: ref32 article-title: CasOT: a genome-wide Cas9/gRNA off-target searching tool publication-title: Bioinformatics doi: 10.1093/bioinformatics/btt764 – volume: 79 start-page: 348 issue: 2 year: 2014 ident: ref12 article-title: Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana publication-title: Plant J doi: 10.1111/tpj.12554 – volume: 19 start-page: 63 issue: 1 year: 2007 ident: ref45 article-title: Stomatal development and patterning are regulated by environmentally responsive mitogen-activated protein kinases in Arabidopsis publication-title: Plant Cell doi: 10.1105/tpc.106.048298 – volume: 57 start-page: 2600 issue: 12 year: 2016 ident: ref8 article-title: Efficient and heritable targeted mutagenesis in mosses using the CRISPR/Cas9 system publication-title: Plant Cell Physiol doi: 10.1093/pcp/pcw173 – volume: 128 start-page: 337 issue: 2 year: 2015 ident: ref21 article-title: AgarTrap-mediated genetic transformation using intact gemmae/gemmalings of the liverwort Marchantia polymorpha L publication-title: J Plant Res doi: 10.1007/s10265-014-0695-2 – volume: 7 start-page: 193 issue: 1 year: 2017 ident: ref47 article-title: CRISPR/Cas9-induced double-strand break repair in Arabidopsis nonhomologous end-joining mutants publication-title: G3 (Bethesda) doi: 10.1534/g3.116.035204 – volume: 11 start-page: 118 issue: 1 year: 2016 ident: ref50 article-title: MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems publication-title: Nat Protoc doi: 10.1038/nprot.2015.140 – volume: 166 start-page: 411 issue: 1 year: 2014 ident: ref33 article-title: Phototropin encoded by a single-copy gene mediates chloroplast photorelocation movements in the liverwort Marchantia polymorpha publication-title: Plant physiology doi: 10.1104/pp.114.245100 – volume: 58 start-page: 1642 issue: 10 year: 2017 ident: ref37 article-title: The roles of the sole activator-type auxin response factor in pattern formation of Marchantia polymorpha publication-title: Plant Cell Physiol doi: 10.1093/pcp/pcx095 – volume: 46 start-page: 401 year: 1908 ident: ref17 article-title: Bryological papers II. The origin of the cupule of Marchantia—contributions from the hull botanical laboratory 120 publication-title: Bot Gaz doi: 10.1086/329782 – volume: 55 start-page: 475 issue: 3 year: 2014 ident: ref25 article-title: CRISPR/Cas9-mediated targeted mutagenesis in the liverwort Marchantia polymorpha L publication-title: Plant Cell Physiol doi: 10.1093/pcp/pcu014 – volume: 13 start-page: 20 year: 2017 ident: ref24 article-title: TALEN-mediated genome-editing approaches in the liverwort Marchantia polymorpha yield high efficiencies for targeted mutagenesis publication-title: Plant Methods doi: 10.1186/s13007-017-0167-5 – volume: 10 start-page: e0138876 issue: 9 year: 2015 ident: ref30 article-title: Development of Gateway binary vector series with four different selection markers for the liverwort Marchantia polymorpha publication-title: PLoS One doi: 10.1371/journal.pone.0138876 – volume: 56 start-page: 41 issue: 1 year: 2015 ident: ref42 article-title: Multigene knockout utilizing off-target mutations of the CRISPR/Cas9 system in rice publication-title: Plant Cell Physiol doi: 10.1093/pcp/pcu154 – volume: 11 start-page: e1005084 issue: 5 year: 2015 ident: ref35 article-title: Auxin-mediated transcriptional system with a minimal set of components is critical for morphogenesis through the life cycle in Marchantia polymorpha publication-title: PLoS Genet doi: 10.1371/journal.pgen.1005084 – volume: 104 start-page: 34 issue: 1 year: 2007 ident: ref31 article-title: Development of series of gateway binary vectors, pGWBs, for realizing efficient construction of fusion genes for plant transformation publication-title: J Biosci Bioeng doi: 10.1263/jbb.104.34 – volume: 346 start-page: 1258096 issue: 6213 year: 2014 ident: ref1 article-title: Genome editing. The new frontier of genome engineering with CRISPR-Cas9 publication-title: Science doi: 10.1126/science.1258096 – volume: 13 start-page: 337 issue: 3 year: 2014 ident: ref41 article-title: RNA polymerase is a powerful torsional motor publication-title: Cell Cycle doi: 10.4161/cc.27508 – volume: 167 start-page: 325 issue: 2 year: 2016 ident: ref15 article-title: Field guide to plant model systems publication-title: Cell doi: 10.1016/j.cell.2016.08.031 – volume: 36 start-page: 1 year: 2016 ident: ref5 article-title: Applying CRISPR/Cas for genome engineering in plants: the best is yet to come publication-title: Curr Opin Plant Biol doi: 10.1016/j.pbi.2016.11.011 – volume: 111 start-page: 4632 issue: 12 year: 2014 ident: ref14 article-title: Multigeneration analysis reveals the inheritance, specificity, and patterns of CRISPR/Cas-induced gene modifications in Arabidopsis publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.1400822111 – volume: 57 start-page: 359 issue: 2 year: 2016 ident: ref52 article-title: Profiling and characterization of small RNAs in the liverwort, Marchantia polymorpha, belonging to the first diverged land plants publication-title: Plant Cell Physiol doi: 10.1093/pcp/pcv182 – volume: 20 start-page: 56 issue: 1 year: 2015 ident: ref44 article-title: Mitogen-activated protein kinase cascades in signaling plant growth and development publication-title: Trends Plant Sci doi: 10.1016/j.tplants.2014.10.001 – volume: 88 start-page: 561 issue: 6 year: 2015 ident: ref26 article-title: Comparison of CRISPR/Cas9 expression constructs for efficient targeted mutagenesis in rice publication-title: Plant Mol Biol doi: 10.1007/s11103-015-0342-x – volume: 57 start-page: 271 issue: 2 year: 2016 ident: ref23 article-title: Conditional gene expression/deletion systems for Marchantia polymorpha using its own heat-shock promoter and Cre/loxP-mediated site-specific recombination publication-title: Plant Cell Physiol doi: 10.1093/pcp/pcv102 – volume: 77 start-page: 167 issue: 1 year: 2013 ident: ref20 article-title: Efficient Agrobacterium-mediated transformation of the liverwort Marchantia polymorpha using regenerating thalli publication-title: Biosci Biotechnol Biochem doi: 10.1271/bbb.120700 – volume: 87 start-page: 143 issue: 1–2 year: 2015 ident: ref27 article-title: Comparative assessments of CRISPR-Cas nucleases’ cleavage efficiency in planta publication-title: Plant Mol Biol doi: 10.1007/s11103-014-0266-x – volume: 26 start-page: 409 issue: 7 year: 2015 ident: ref2 article-title: CRISPR-Cas9: Prospects and challenges publication-title: Hum Gene Ther doi: 10.1089/hum.2015.29002.fzh – volume: 57 start-page: 262 issue: 2 year: 2016 ident: ref18 article-title: Molecular genetic tools and techniques for Marchantia polymorpha research publication-title: Plant Cell Physiol doi: 10.1093/pcp/pcv097 – volume: 3 start-page: 1532 year: 2013 ident: ref22 article-title: Homologous recombination-mediated gene targeting in the liverwort Marchantia polymorpha L publication-title: Sci Rep doi: 10.1038/srep01532 – volume: 337 start-page: 816 issue: 6096 year: 2012 ident: ref3 article-title: A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity publication-title: Science doi: 10.1126/science.1225829 – volume: 3 start-page: e161 year: 2014 ident: ref38 article-title: Pol III promoters to express small RNAs: delineation of transcription initiation publication-title: Mol Ther Nucleic Acids doi: 10.1038/mtna.2014.12 – volume: 32 start-page: 947 issue: 9 year: 2014 ident: ref13 article-title: Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew publication-title: Nat Biotechnol doi: 10.1038/nbt.2969 – volume: 114 start-page: 13567 issue: 51 year: 2017 ident: ref11 article-title: Efficient targeted DNA editing and replacement in Chlamydomonas reinhardtii using Cpf1 ribonucleoproteins and single-stranded DNA publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.1710597114 – volume: 50 start-page: 151 issue: 1 year: 1968 ident: ref29 article-title: Nutrient requirements of suspension cultures of soybean root cells publication-title: Exp Cell Res doi: 10.1016/0014-4827(68)90403-5 – volume: 17 start-page: 979 issue: 1 year: 2016 ident: ref49 article-title: Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ publication-title: BMC Genomics doi: 10.1186/s12864-016-3331-9 – volume: 56 start-page: 389 issue: 3 year: 2015 ident: ref7 article-title: Genome editing with engineered nucleases in plants publication-title: Plant Cell Physiol doi: 10.1093/pcp/pcu170 – volume: 8 issue: 12 year: 2016 ident: ref4 article-title: Genome-editing technologies: principles and applications publication-title: Cold Spring Harb Perspect Biol – year: 2016 ident: ref9 article-title: Simple and efficient targeting of multiple genes through CRISPR-Cas9 in Physcomitrella patens publication-title: G3 (Bethesda) – volume: 32 start-page: 279 issue: 3 year: 2014 ident: ref40 article-title: Improving CRISPR-Cas nuclease specificity using truncated guide RNAs publication-title: Nat Biotechnol doi: 10.1038/nbt.2808 – volume: 11 start-page: e1005207 issue: 5 year: 2015 ident: ref36 article-title: A simple auxin transcriptional response system regulates multiple morphogenetic processes in the liverwort Marchantia polymorpha publication-title: PLoS Genet doi: 10.1371/journal.pgen.1005207 – year: 2013 ident: ref34 article-title: Comparison of the MpEF1α and CaMV35 promoters for application in Marchantia polymorpha overexpression studies publication-title: Transgenic Res – volume: 49 start-page: 1084 issue: 7 year: 2008 ident: ref19 article-title: Agrobacterium-mediated transformation of the haploid liverwort Marchantia polymorpha L., an emerging model for plant biology publication-title: Plant Cell Physiol doi: 10.1093/pcp/pcn085 – year: 2009 ident: ref28 article-title: Overview: generation of gene knockout mice publication-title: Curr Protoc Cell Biol doi: 10.1002/0471143030.cb1912s44 – volume: 7 start-page: 301 issue: 7 year: 2002 ident: ref43 article-title: Mitogen-activated protein kinase cascades in plants: a new nomenclature publication-title: Trends Plant Sci doi: 10.1016/S1360-1385(02)02302-6 – volume: 57 start-page: 339 issue: 2 year: 2016 ident: ref51 article-title: Identification of miRNAs and their targets in the liverwort Marchantia polymorpha by integrating RNA-seq and degradome analyses publication-title: Plant Cell Physiol doi: 10.1093/pcp/pcw020 – volume: 171 start-page: 287 issue: 2 year: 2017 ident: ref16 article-title: Insights into land plant evolution garnered from the Marchantia polymorpha genome publication-title: Cell doi: 10.1016/j.cell.2017.09.030 – volume: 339 start-page: 819 issue: 6121 year: 2013 ident: ref48 article-title: Multiplex genome engineering using CRISPR/Cas systems publication-title: Science doi: 10.1126/science.1231143 – volume: 25 start-page: 4075 issue: 10 year: 2013 ident: ref39 article-title: Essential role of the E3 ubiquitin ligase nopperabo1 in schizogenous intercellular space formation in the liverwort Marchantia polymorpha publication-title: Plant Cell doi: 10.1105/tpc.113.117051 – volume: 3 start-page: 17107 year: 2017 ident: ref6 article-title: Progress and prospects in plant genome editing publication-title: Nat Plants doi: 10.1038/nplants.2017.107 – volume: 29 start-page: 2498 issue: 10 year: 2017 ident: ref10 article-title: Targeting of photoreceptor genes in Chlamydomonas reinhardtii via zinc-finger nucleases and CRISPR/Cas9 publication-title: Plant Cell doi: 10.1105/tpc.17.00659 |
| SSID | ssj0053866 |
| Score | 2.6149533 |
| Snippet | Marchantia polymorpha is one of the model species of basal land plants. Although CRISPR/Cas9-based genome editing has already been demonstrated for this plant,... Marchantia polymorpha is one of the model species of basal land plants. Although CRISPR/Cas9-based genome editing has already been demonstrated for this plant,... |
| SourceID | plos doaj pubmedcentral proquest pubmed crossref |
| SourceType | Open Website Open Access Repository Aggregation Database Index Database Enrichment Source |
| StartPage | e0205117 |
| SubjectTerms | Biology and life sciences Cell cycle Combinatorial analysis Complementation CRISPR Editing Efficiency Engineering and Technology Feasibility studies Flowers & plants Functional analysis Gene expression Genetic analysis Genetics Genome editing Genomes Genomics Kinases Marchantia polymorpha Mutation Optimization Proteins Research and Analysis Methods RNA polymerase Sequences Trends |
| SummonAdditionalLinks | – databaseName: DOAJ Directory of Open Access Journals dbid: DOA link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9QwELZQT1wQ5dWFgozEAQ7pJrZjx0dYtSpIRahQqbdoHNtipW2yIqkQ_56ZOLvaRZV64RqPE3senpmM_Zmxd6JwLkRdZs4IlalodWalhixGa6CylZNjBf_iqz6_Ul-uy-udq75oT1iCB06Mm9sotBBegAKtbAEgnQ05KEdAX2jPtPrmNt8kU2kNRivWejooJ00xn-Rysu7acIIBEkYZZs8RjXj9hG-66vq7Ys1_t0zu-KCzx-zRFDzyj2nQh-xBaJ-ww8k8e_5-wpD-8JT9Ph2hIfA1fHH5-fu3y_kCepuRz_KccFlvAke3RXueObSeL4ee79Sy-dBxTJT9Mv0ppB502BFJE4QJX7b8YoRZanGF4Otu9eemQ4nBM3Z1dvpjcZ5NdyxkTWnyIYvexiaQRABNu_EYsUSTK2MKJULQlW9E0KKQ3pRNKZvchFJ7IV0JAYom9_I5O2iRq0eM5wWEYKMBpytlpQcNERMkFfMKGm3cjMkNw-tmAiCnezBW9VhVM5iIJDbWJKZ6EtOMZdte6wTAcQ_9J5Lllpbgs8cHqFT1pFT1fUo1Y0ekCZsP9DXh4OuSwNRm7HijHXc3v902o6FS9QXa0N0mGlvSQeEZe5GUaTtIjCOMrQQO3uyp2d4s9lva5c8RDFyj9he6evk_pv2KPcR4sEqu-ZgdDL9uw2uMuQb3ZjSvv7PcLKE priority: 102 providerName: Directory of Open Access Journals – databaseName: ProQuest Central dbid: BENPR link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwhV3fb9MwELZG98ILYvxa2EBG4gEesiZOYscPCLGq00BaNRUm7S26xDZU6pJu6YT477lLnLKiCV7jS-L47nyfc_Z3jL0VcVlaJ7OwVCINU6dlqBMJoXNaQa7zMuky-GczeXqRfrnMLnfYbDgLQ9sqhzmxm6hNU9E_8jExkcuM6Kw-rq5DqhpF2dWhhAb40grmQ0cx9oDtonwWjdju8XR2Ph_mZvRuKf0BukTFY6-vo1VT2yMETog-1FaA6nj8ifd02bT3YdC_t1LeiU0nj9kjDyr5p94K9tiOrZ-wPe-2LX_nuaXfP2U_px1lBD6GT-afv57PxxNodUixzHDia72yHMMZ7YXmUBu-WLf8To6brxuOC2iz6P8g0h10CBJFe2oTvqj5WUe_hKMEfNUsf101qEl4xi5Opt8mp6GvvRBWmYrWoTPaVZY0BejylUEk41SUKhWnwlqZm0pYKeLEqKzKkipSNpNGJGUGFuIqMslzNqpxVPcZj2KwVjsFpcxTnRiQ4HDhlLooh0qqMmDJMOBF5YnJqT7GsuiybQoXKP0wFqSmwqspYOHmrlVPzPEf-WPS5UaWaLW7C83N98J7aaGdkEIYASnIVMcASaltBGlJrHIYPAK2T5YwvKAt_lhlwA4H67i_-c2mGR2YsjJQ2-a2l9EZHSAO2IvemDadRHyhdC6w82rLzLa-YrulXvzoSMKlQCQo85f_7tYBe4gIMO-D8SEbrW9u7StEWevytXed35VhK4I priority: 102 providerName: ProQuest |
| Title | Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/30379827 https://www.proquest.com/docview/2127655563 https://www.proquest.com/docview/2127952519 https://pubmed.ncbi.nlm.nih.gov/PMC6209168 https://doaj.org/article/9f2622d2a4a6491aa3b9e0a4b4656000 http://dx.doi.org/10.1371/journal.pone.0205117 |
| Volume | 13 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV3Nb9MwFLf2ceGCGF8LG5WROMAhJXEcOz4gxKqWgbRpKlTqLXJiGyp1Sdd0gv33vOek1YqKEJccajtN30ff7-XZv0fIaxYXhXUiDQvJeMidEqFKhA6dU1JnKisSX8G_uBTnE_5lmk73yLpnayfAZmdqh_2kJst5_9fN3Qdw-Pe-a4OM14v6i7qyfYA_gCHkPjn0FSPczMc3dQXwbl-9RNQSChYl3WG6v91lK1h5Tn_kQJ3XzS48-ue2yntxavSIPOwAJv3YWsQR2bPVY3LUuXBD33Q802-fkJ9DTx8Bt6GD8eevV-N3A92oEOOaocjdem0phDbcF011Zehs1dB79W66qikk02bWvk3EFXggEqa2NCd0VtELT8VUwb8IXdTzu-satKqfkslo-G1wHnZ9GMIyldEqdEa50qLWNLh_aQDVOBlxKWPOrBWZKZkVLE6MTMs0KSNpU2FYUqTa6riMTPKMHFQg1WNCo1hbq5zUhci4SowW2kESxV2U6VLIIiDJWuB52ZGUY6-Mee4rbxKSlVaMOaop79QUkHCzatGSdPxj_hnqcjMXKbb9B_Xye955bK4cE4wZprkWXMVaJ4WykeYFMsxBIAnIMVrC-guaHLnyRYqEawE5XVvH7uFXm2FwZqzQ6MrWt-0cleJh4oA8b41p85CANaTKGDy83DKzrV-xPVLNfnjCcLB0yAKyF_8pphPyAOBh1kbqU3KwWt7alwDBVkWP7MuphGs2iPE6-tQjh2fDy6txz7_U6Hmv-w2fbzfx |
| linkProvider | Scholars Portal |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV3fb9MwELZG9wAviPFrhQFGAgkesiZOYscPE2KlU8vWahqbtLfMiW2o1CVl6TTtn-Nv4y5xyoomeNpr7aSO73z32ef7jpB3LMgyY3nsZYJFXmQl92TIlWetFCqRSRbWEfzxhA9Poq-n8eka-dXmwuC1ytYm1oZalzmekfeQiZzHSGf1af7Tw6pRGF1tS2goV1pB79QUYy6xY99cX8EWrtoZfQF5v2dsb3DcH3quyoCXx8JfeFZLmxsckwLlzjX4bCv8SIggYsbwROfMcBaEWsR5HOa-MDHXLMxiZVSQ-zqE994j6xEeoHTI-u5gcnjU-gKwJpy7hL1QBD2nH9vzsjDbANQA7YgVh1jXDUCe1VlZ3YZ5_766ecMX7j0iDx2IpZ8brdsga6Z4TDacmajoB8dl_fEJuRrUFBXwGto_Gn07POr1VSU99J2aIj_suaHgPvHuNVWFptNFRW_E1OmipLBh19PmxBKfwKRL6NpQqdBpQcc13RNIRdF5Obs-L0Fz1FNycidSeEY6BczqJqF-oIyRVqiMJ5EMteLKwkYtsn6ici6yLgnbCU9zR4SO9ThmaR3dE7AhaqYxRTGlTkxd4i2fmjdEIP_pv4uyXPZFGu_6h_Lie-qsQiot44xppiLFIxkoFWbS-CrKkMUOnFWXbKImtH9QpX9WQZdstdpxe_PbZTMYDIwCqcKUl00fGWPCcpc8b5RpOUjAM0ImDAYvVtRs5StWW4rpj5qUnDNAnjx58e9hvSH3h8fjg_RgNNl_SR4A-kwaILBFOouLS_MKEN4ie-2WESVnd71yfwMpkGjN |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV3db9MwELfGJiFeEONr3QYYCSR4yJo4iR0_TIh1rVbGqqowaW_BiW2o1CVl6TTtX-Sv4i5xyoomeNprbCeO73wfPt_vCHnDgiwzlsdeJljkRVZyT4ZcedZKoRKZZGEdwT8Z8aPT6NNZfLZGfrW5MHitspWJtaDWZY5n5F1EIucxwll1rbsWMT4cfJj_9LCCFEZa23IaypVZ0Ps13JhL8jg211fgzlX7w0Og_VvGBv2vvSPPVRzw8lj4C89qaXOD81PA6LkG_W2FHwkRRMwYnuicGc6CUIs4j8PcFybmmoVZrIwKcl-H8N57ZEOA1gdHcOOgPxpPWr0AkoVzl7wXiqDreGVvXhZmD4w2sHzEinKsawgg5uqsrG6zf_--xnlDLw4ekYfOoKUfGw7cJGumeEw2ncio6DuHa_3-Cbnq13AV8Bramwy_jCfdnqqkh3pUU8SKPTcUVCnew6aq0HS6qOiN-DpdlBScdz1tTi9xBCZgQtcGVoVOC3pSQz8BVRSdl7Pr8xK4SD0lp3dChWdkvYBV3SLUD5Qx0gqV8SSSoVZcWXDaIusnKuci65CwXfA0d6DoWJtjltaRPgHOUbOMKZIpdWTqEG85at6Agvyn_wHSctkXIb3rB-XF99RJiFRaxhnTTEWKRzJQKsyk8VWUIaIdKK4O2UJOaD9QpX92RIfsttxxe_PrZTMID4wIqcKUl00fGWPycoc8b5hpOUmwbYRMGExerLDZyl-sthTTHzVAOWdghfJk-9_TekXuww5OPw9HxzvkARiiSWMT7JL1xcWleQHG3iJ76XYRJd_ueuP-BvTRbRE |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Efficient+CRISPR%2FCas9-based+genome+editing+and+its+application+to+conditional+genetic+analysis+in+Marchantia+polymorpha&rft.jtitle=PloS+one&rft.au=Sugano%2C+Shigeo+S.&rft.au=Nishihama%2C+Ryuichi&rft.au=Shirakawa%2C+Makoto&rft.au=Takagi%2C+Junpei&rft.date=2018-10-31&rft.issn=1932-6203&rft.eissn=1932-6203&rft.volume=13&rft.issue=10&rft.spage=e0205117&rft_id=info:doi/10.1371%2Fjournal.pone.0205117&rft.externalDBID=n%2Fa&rft.externalDocID=10_1371_journal_pone_0205117 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1932-6203&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1932-6203&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1932-6203&client=summon |