卵形鲳鲹Tf、TNFα和C-Lys的组织分布及对美人鱼发光杆菌感染的响应
研究了卵形鲳鲹（Trachinotus ovatus）中免疫相关因子转铁蛋白（Tf）、肿瘤坏死因子α（TNFα）、C-型溶菌酶（C-Lys）的组织表达分布,并分析了这3种基因在人工感染美人鱼发光杆菌杀鱼亚种（Photobacterium damselae subsp.piscicida）后在卵形鲳鲹肾脏中的表达变化,探讨其对美人鱼发光杆菌感染的反应。结果显示,感染后第48小时卵形鲳鲹累计死亡率最高（76.4%）;卵形鲳鲹肾脏中细菌数量从第3～第96小时一直处于下降趋势,从1.07×10^6CFU·g^-1下降到1.29×10^3CFU·g^-1。健康卵形鲳鲹中Tf和TNFα在肝脏中表达量最高,...
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| Published in | 南方水产科学 Vol. 13; no. 2; pp. 77 - 84 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
上海海洋大学水产与生命学院,上海201306%中国水产科学研究院南海水产研究所,农业部水产品加工重点实验室,广东 广州 510300
2017
中国水产科学研究院南海水产研究所,农业部水产品加工重点实验室,广东 广州 510300 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 2095-0780 |
| DOI | 10.3969/j.issn.2095-0780.2017.02.010 |
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| Abstract | 研究了卵形鲳鲹（Trachinotus ovatus）中免疫相关因子转铁蛋白（Tf）、肿瘤坏死因子α（TNFα）、C-型溶菌酶（C-Lys）的组织表达分布,并分析了这3种基因在人工感染美人鱼发光杆菌杀鱼亚种（Photobacterium damselae subsp.piscicida）后在卵形鲳鲹肾脏中的表达变化,探讨其对美人鱼发光杆菌感染的反应。结果显示,感染后第48小时卵形鲳鲹累计死亡率最高（76.4%）;卵形鲳鲹肾脏中细菌数量从第3～第96小时一直处于下降趋势,从1.07×10^6CFU·g^-1下降到1.29×10^3CFU·g^-1。健康卵形鲳鲹中Tf和TNFα在肝脏中表达量最高,CLys在头肾中表达量最高。美人鱼发光杆菌感染后卵形鲳鲹肾脏中Tf表达量在第3～第12小时实验组高于对照组,其中第6小时的表达量最高,是对照组的17.99倍;TNFα在感染后的第3、第6、第24小时实验组的表达量高于对照组,第24小时组相对于对照组的表达量最高,是对照组的6.05倍。C-Lys在感染后感染组表达量均低于对照组。以上结果表明,美人鱼发光杆菌感染能促进卵形鲳鲹肾脏组织中Tf、TNFα的表达,而抑制C-Lys的表达。 |
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| AbstractList | 研究了卵形鲳鲹（Trachinotus ovatus）中免疫相关因子转铁蛋白（Tf）、肿瘤坏死因子α（TNFα）、C-型溶菌酶（C-Lys）的组织表达分布,并分析了这3种基因在人工感染美人鱼发光杆菌杀鱼亚种（Photobacterium damselae subsp.piscicida）后在卵形鲳鲹肾脏中的表达变化,探讨其对美人鱼发光杆菌感染的反应。结果显示,感染后第48小时卵形鲳鲹累计死亡率最高（76.4%）;卵形鲳鲹肾脏中细菌数量从第3～第96小时一直处于下降趋势,从1.07×10^6CFU·g^-1下降到1.29×10^3CFU·g^-1。健康卵形鲳鲹中Tf和TNFα在肝脏中表达量最高,CLys在头肾中表达量最高。美人鱼发光杆菌感染后卵形鲳鲹肾脏中Tf表达量在第3～第12小时实验组高于对照组,其中第6小时的表达量最高,是对照组的17.99倍;TNFα在感染后的第3、第6、第24小时实验组的表达量高于对照组,第24小时组相对于对照组的表达量最高,是对照组的6.05倍。C-Lys在感染后感染组表达量均低于对照组。以上结果表明,美人鱼发光杆菌感染能促进卵形鲳鲹肾脏组织中Tf、TNFα的表达,而抑制C-Lys的表达。 Q786; 研究了卵形鲳鲹(Trachinotus ovatus)中免疫相关因子转铁蛋白(Tf)、肿瘤坏死因子α(TNFα)、C-型溶菌酶(C-Lys)的组织表达分布,并分析了这3种基因在人工感染美人鱼发光杆菌杀鱼亚种(Photobacterium damselae subsp.piscicida )后在卵形鲳鲹肾脏中的表达变化,探讨其对美人鱼发光杆菌感染的反应.结果显示,感染后第48小时卵形鲳鲹累计死亡率最高(76.4%);卵形鲳鲹肾脏中细菌数量从第3~第96小时一直处于下降趋势,从1.07×10.6 CFU·g-1下降到1.29×10.3 CFU·g-1.健康卵形鲳鲹中Tf和TNFα在肝脏中表达量最高,C-Lys在头肾中表达量最高.美人鱼发光杆菌感染后卵形鲳鲹肾脏中Tf表达量在第3~第12小时实验组高于对照组,其中第6 小时的表达量最高,是对照组的17.99倍;TNFα在感染后的第3、第6、第24小时实验组的表达量高于对照组,第24小时组相对于对照组的表达量最高,是对照组的6.05倍.C-Lys在感染后感染组表达量均低于对照组.以上结果表明,美人鱼发光杆菌感染能促进卵形鲳鲹肾脏组织中Tf、TNFα的表达,而抑制C-Lys的表达. |
| Abstract_FL | The study investigates the tissue expression of transferrin (Tf),tumor necrosis factor alpha (TNFα) and C-type lysozyme (C-Lys) in healthy Trachinotus ovatus after it was infected with Photobacterium damselae subsp.piscicida in kidney.The results show that after infection,the accumulative mortalities of T.ovatus reached the maximum in 48 h (76.4%);and the colony of P.damselae in T.ovatus' kidney continued to decline in 3~96 h from 1.07×10.6 CFU·g-1to 1.29×10.3 CFU·g-1.The Tf and TNFα expressions were the highest in the liver,and the C-Lys expression was the highest in the head kidney in healthy T.ovatus.After TOS1 infected,the Tf expression in 3~12 h in test group was higher than that of control and was the highest in 6 h (17.99 times as high as that of control).The TNFα expression of the test group was higher than that of the control after infection of 3 h,6 h and 24 h.The TNFα expression was the highest in 24 h (6.05 times higher than that of control).The C-Lys expression in infection group was lower than that in control in 3~96 h.The study indicates that the Tf and TNFα expressions can be promoted while C-Lys will be suppressed after TOS1 infection in T.ovatus' kidney. |
| Author | 宋沙沙 马红玲 冯娟 苏友禄 程长洪 郭志勋 |
| AuthorAffiliation | 中国水产科学研究院南海水产研究所,农业部水产品加工重点实验室,广东广州510300 上海海洋大学水产与生命学院,上海201306 |
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| Author_FL | SU Youlu MA Hongling FENG Juan SONG Shasha CHENG Changhong GUO Zhixun |
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| DOI | 10.3969/j.issn.2095-0780.2017.02.010 |
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| DocumentTitleAlternate | Tissue distribution and response to infection of Photobacterium damselae subsp, piscicida of Tf, TNFa and C-Lys in Trachinotus ovatus |
| DocumentTitle_FL | Tissue distribution and response to infection of Photobacterium damselae subsp.piscicida of Tf,TNFα and C-Lys in Trachinotus ovatus |
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| Keywords | 肿瘤坏死因子α Trachinotus ovatus tumor necrosis factor alpha C-type lysozyme C-型溶菌酶 转铁蛋白 transferrin 基因表达 卵形鲳鲹 gene expression |
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| Notes | SONG Shasha1,2 , MA Hongling1 , FENG Juan1, SU Youlu1, CHENG Changhong1 , GUO Zhixun1（1. Key Lab. of Aquatic Product Processing, Ministry of Agriculture; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; 2. College of Fisheries and Life, Shanghai Ocean University, Shanghai 201306, China） Trachinotus ovatus; transferrin; tumor necrosis factor alpha; C-type lysozyme; gene expression The study investigates the tissue expression of transferrin （Tf）, tumor necrosis factor alpha （TNFα） and C-type lysozyme （C-Lys） in healthy Trachinotus ovatus after it was infected with Photobacterium damselae subsp. piscicida in kidney. The results show that after infection, the accumulative mortalities of T.ovatus reached the maximumin 48 h （76.4%）; and the colony of P.damselae in T.ovatus′ kidney continued to decline in 3~96 h from 1.07×10^6 CFU·g^-1 to 1.29 ×10^3 CFU·g^-1. The Tf and TNFα expressions were the highest in the liver, and the C-Lys expression was the highest in |
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| PublicationTitle | 南方水产科学 |
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| Publisher | 上海海洋大学水产与生命学院,上海201306%中国水产科学研究院南海水产研究所,农业部水产品加工重点实验室,广东 广州 510300 中国水产科学研究院南海水产研究所,农业部水产品加工重点实验室,广东 广州 510300 |
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| Snippet | 研究了卵形鲳鲹（Trachinotus ovatus）中免疫相关因子转铁蛋白（Tf）、肿瘤坏死因子α（TNFα）、C-型溶菌酶（C-Lys）的组织表达分布,并分析了这3种基因在人工感染美人鱼发光... Q786; 研究了卵形鲳鲹(Trachinotus ovatus)中免疫相关因子转铁蛋白(Tf)、肿瘤坏死因子α(TNFα)、C-型溶菌酶(C-Lys)的组织表达分布,并分析了这3种基因在人工感染美人鱼发光杆... |
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| SubjectTerms | C-型溶菌酶 卵形鲳鲹 基因表达 肿瘤坏死因子α 转铁蛋白 |
| Title | 卵形鲳鲹Tf、TNFα和C-Lys的组织分布及对美人鱼发光杆菌感染的响应 |
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